CN106834100A - A kind of animal embryo splits ware - Google Patents

A kind of animal embryo splits ware Download PDF

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Publication number
CN106834100A
CN106834100A CN201710240677.6A CN201710240677A CN106834100A CN 106834100 A CN106834100 A CN 106834100A CN 201710240677 A CN201710240677 A CN 201710240677A CN 106834100 A CN106834100 A CN 106834100A
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CN
China
Prior art keywords
embryo
segmentation
dividing grooves
indenture
fixed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710240677.6A
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Chinese (zh)
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CN106834100B (en
Inventor
曹鸿国
陈洁梅
陈宏权
王桂军
曾新国
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mingguang Modern Agricultural Science And Technology Cooperative Extension Service Center
Anhui Agricultural University AHAU
Original Assignee
Mingguang Modern Agricultural Science And Technology Cooperative Extension Service Center
Anhui Agricultural University AHAU
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Application filed by Mingguang Modern Agricultural Science And Technology Cooperative Extension Service Center, Anhui Agricultural University AHAU filed Critical Mingguang Modern Agricultural Science And Technology Cooperative Extension Service Center
Priority to CN201710240677.6A priority Critical patent/CN106834100B/en
Publication of CN106834100A publication Critical patent/CN106834100A/en
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Publication of CN106834100B publication Critical patent/CN106834100B/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish

Abstract

The invention discloses a kind of animal embryo segmentation ware,Including ware body and upper lid,Upper lid is connected on ware body,Ware body is provided with several dividing grooves,The centre position of dividing grooves is provided with segmentation indenture,The bottom cross section for splitting indenture is triangle,The drift angle of triangle is located on the center line of dividing grooves,Segmentation indenture two ends contact with dividing grooves upper surface,Form the opening of triangle,Segmentation indenture is provided with multiple fixed convex epireliefs in dividing grooves,Several fixing tooths are evenly equipped with fixed convex epirelief,Fixing tooth is inclined to fixed convex epirelief one end,The present invention,It is designed with fixed convex epirelief,Embryo is fixed convenient positioning by the fixing tooth on fixed convex epirelief,Convex epirelief side is fixed due to fixed teeth directional to incline,So in segmentation,Fixing tooth can just tangle the oolemma of embryo periphery,Embryo is again not easily shifted,Complete embryo's cutting.

Description

A kind of animal embryo splits ware
Technical field
Technical field the invention belongs to split ware, specially a kind of animal embryo segmentation ware.
Background technology
Because an embryonated egg is when embryo is developed into, to experience a fertilized egg cell and be split into two, two divisions Such process for four, four are split into eight ..., and embryo's composition cell of animal early stage has totipotency, it is theoretical Each composition cell of upper body early embryo is respectively provided with the ability for developing into a newborn individual, therefore using modern embryo engineering Technology is split animal body early embryo, and splitting each section embryo for being formed can develop to a new living individual, The reproductive efficiency of outstanding animal can be thus greatly enhanced, meanwhile, heritability can simultaneously be obtained by this technology Consistent outstanding animal population.But current animal embryo segmentation lacks special, effective, easily operation device, animal embryo Segmentation is more to be carried out using micromanipulation systems, and micromanipulation system has expensive, and maintenance cost is high, while needing skilled Operating technology personnel carry out the work.Therefore, a kind of simple, convenient, inexpensive special operation device having for animal embryo Effect segmentation is particularly significant.
The content of the invention
It is an object of the invention to provide a kind of animal embryo segmentation ware, the different animal embryo of diameter is adapted to, Operating personnel are singlehanded can to complete embryo's cutting, and during embryo separating, fixing tooth can be fixedly secured the saturating of embryo periphery Oolemma, embryo is evenly and rapidly cut using embryo separating knife, and whole operation is convenient, fast, and operating personnel are required with technology water Mean pole is low, and segmentation ware is cheap, and a ware is multiplex, easy to use.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is:
A kind of animal embryo splits ware, including ware body and upper lid, and upper lid is connected on ware body, and ware body is provided with several segmentations Groove, the centre position of dividing grooves is provided with segmentation indenture, and the bottom cross section for splitting indenture is triangle, the drift angle of triangle On the center line of dividing grooves, segmentation indenture two ends contact with dividing grooves upper surface, form the opening of triangle, are dividing Cut and segmentation indenture is provided with multiple fixed convex epireliefs on groove, several fixing tooths, fixing tooth are evenly equipped with fixed convex epirelief Inclined to fixed convex epirelief one end, the volume of fixing tooth is tapered into from fixed convex epirelief one end to the other end.
Further, fixing tooth material is plastics, integrated with fixed convex epirelief to make.
The beneficial effects of the invention are as follows:
Fixed convex epirelief is designed with, embryo is fixed convenient positioning by the fixing tooth on fixed convex epirelief, because fixed teeth directional is consolidated Determine the inclination of convex epirelief side, so in segmentation, fixing tooth can just tangle the oolemma of embryo periphery, and embryo is again not easily shifted, Complete embryo's cutting.
Brief description of the drawings
The present invention is further detailed explanation with reference to the accompanying drawings and detailed description.
Fig. 1 is schematic diagram of the present invention.
Fig. 2 is ware body schematic diagram of the present invention.
Fig. 3 is ware body sectional view of the present invention.
Fig. 4 is dividing grooves schematic diagram of the present invention.
Fig. 5 is dividing grooves sectional view of the present invention.
Specific embodiment
Embodiments of the invention are described in detail below in conjunction with accompanying drawing, but the present invention can be defined by the claims Multitude of different ways with covering is implemented.
As shown in drawings, a kind of animal embryo segmentation ware, including ware body 1 and upper lid 2, upper lid 2 are connected on ware body 1, ware Body 2 is provided with several dividing grooves 10, and the centre position of dividing grooves 10 is provided with segmentation indenture 11, splits the bottom transverse of indenture 11 Section is triangle, and the drift angle of triangle is located on the center line of dividing grooves 10, is so cut along center line, can be with Embryo is divided into two parts, the segmentation two ends of indenture 11 contact with the upper surface of dividing grooves 10, form the opening 12 of triangle, behaviour Author can be operated along opening 12, very convenient, and segmentation indenture 11 is provided with multiple in dividing grooves 10 Fixed convex epirelief 13, is evenly equipped with several fixing tooths 14 on fixed convex epirelief 13, fixing tooth 14 is inclined to fixed one end of convex epirelief 13, Gu The material of fixed tooth 14 is plastics, and plastic material is that hardness is small, integrated with fixed convex epirelief 13 to make, the size design of fixing tooth 14 It is sufficiently small, so can not only catch on embryo but also be unlikely to damage embryo, the volume of fixing tooth 14 is from fixed one end of convex epirelief 13 to another One end tapers into, and when animal embryo is split, lower knife end is the minimum one end of the volume of fixing tooth 14 to such operating personnel, by Gradually increase in the volume of fixing tooth 14, fixing tooth 14 is improved to animal embryo fixed effect, the effect of stationary positioned is obvious.
In design, ware body 1 is suitable for reading to can be designed as two kinds of specifications of 6cm × 6cm or 10cm × 10cm, meanwhile, split recessed Groove 10 is designed as the hemisphere of 100~200 μ m diameters, can so place single embryo, and entire row is arranged with parallel many on ware body 1 Individual dividing grooves 10, realize simultaneously splitting multiple embryos.
Fixed convex epirelief is designed with, embryo is fixed convenient positioning by the fixing tooth on fixed convex epirelief, because fixed teeth directional is consolidated Determine the inclination of convex epirelief side, so in segmentation, fixing tooth can just tangle the oolemma of embryo periphery, and embryo is again not easily shifted, Fixed teeth material pair, completes embryo's cutting.
Invention described above implementation method, is not intended to limit the scope of the present invention., any in the present invention Spirit and principle within modification, equivalent and the improvement made etc., should be included in claim protection model of the invention Within enclosing.

Claims (2)

1. a kind of animal embryo splits ware, it is characterised in that including ware body and upper lid, upper lid is connected on ware body, is set on ware body The centre position for having several dividing grooves, dividing grooves is provided with segmentation indenture, and the bottom cross section for splitting indenture is triangle, three Angular drift angle is located on the center line of dividing grooves, and segmentation indenture two ends contact with dividing grooves upper surface, form triangle Opening, segmentation indenture is provided with multiple fixed convex epireliefs in dividing grooves, being evenly equipped with several on fixed convex epirelief consolidates Fixed tooth, fixing tooth is inclined to fixed convex epirelief one end, and the volume of fixing tooth is tapered into from fixed convex epirelief one end to the other end.
2. a kind of animal embryo splits ware according to claim 1, it is characterised in that fixing tooth material is plastics, with fixation Convex epirelief integration makes.
CN201710240677.6A 2017-04-13 2017-04-13 Animal embryo segmentation dish Active CN106834100B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710240677.6A CN106834100B (en) 2017-04-13 2017-04-13 Animal embryo segmentation dish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710240677.6A CN106834100B (en) 2017-04-13 2017-04-13 Animal embryo segmentation dish

Publications (2)

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CN106834100A true CN106834100A (en) 2017-06-13
CN106834100B CN106834100B (en) 2023-09-29

Family

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5154188A (en) * 1991-05-09 1992-10-13 The Wolf Foundation Method and device for dividing embryos
JPH10244170A (en) * 1997-03-04 1998-09-14 Nikon Corp Substrate for dividing fine matter and its production
US6448069B1 (en) * 2000-06-09 2002-09-10 Genx International Corp. Embryo-culturing apparatus and method
JP2012029686A (en) * 2010-06-30 2012-02-16 Dainippon Printing Co Ltd Method for producing embryo by in vitro culture, and method, device, and system for selecting embryo
CN203033887U (en) * 2013-01-11 2013-07-03 中南大学 Cutting tool of special capillary glass tube for gamete and embryo operation
CN203683554U (en) * 2014-02-24 2014-07-02 中国农业科学院特产研究所 Oocyte and embryo culture device
CN204981870U (en) * 2015-09-16 2016-01-20 上海科华检验医学产品有限公司 Pick up ovum ware
CN105349485A (en) * 2015-12-15 2016-02-24 广西大学 Improved method for cutting water buffalo blastula by bare hands and cutting fluid
CN206692645U (en) * 2017-04-13 2017-12-01 明光现代农业科技合作推广服务中心 A kind of animal embryo splits ware

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5154188A (en) * 1991-05-09 1992-10-13 The Wolf Foundation Method and device for dividing embryos
JPH10244170A (en) * 1997-03-04 1998-09-14 Nikon Corp Substrate for dividing fine matter and its production
US6448069B1 (en) * 2000-06-09 2002-09-10 Genx International Corp. Embryo-culturing apparatus and method
JP2012029686A (en) * 2010-06-30 2012-02-16 Dainippon Printing Co Ltd Method for producing embryo by in vitro culture, and method, device, and system for selecting embryo
CN203033887U (en) * 2013-01-11 2013-07-03 中南大学 Cutting tool of special capillary glass tube for gamete and embryo operation
CN203683554U (en) * 2014-02-24 2014-07-02 中国农业科学院特产研究所 Oocyte and embryo culture device
CN204981870U (en) * 2015-09-16 2016-01-20 上海科华检验医学产品有限公司 Pick up ovum ware
CN105349485A (en) * 2015-12-15 2016-02-24 广西大学 Improved method for cutting water buffalo blastula by bare hands and cutting fluid
CN206692645U (en) * 2017-04-13 2017-12-01 明光现代农业科技合作推广服务中心 A kind of animal embryo splits ware

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
马保华 等: "生产条件下安哥拉山羊胚胎徒手分割研究", vol. 18, no. 1, pages 1 *

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