CN106834100B - Animal embryo segmentation dish - Google Patents

Animal embryo segmentation dish Download PDF

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Publication number
CN106834100B
CN106834100B CN201710240677.6A CN201710240677A CN106834100B CN 106834100 B CN106834100 B CN 106834100B CN 201710240677 A CN201710240677 A CN 201710240677A CN 106834100 B CN106834100 B CN 106834100B
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China
Prior art keywords
dividing
embryo
recess
apart
fixed
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CN201710240677.6A
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Chinese (zh)
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CN106834100A (en
Inventor
曹鸿国
陈洁梅
陈宏权
王桂军
曾新国
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Mingguang Modern Agricultural Technology Cooperation Extension Services Center
Anhui Agricultural University AHAU
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Mingguang Modern Agricultural Technology Cooperation Extension Services Center
Anhui Agricultural University AHAU
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses an animal embryo dividing dish which comprises a dish body and an upper cover, wherein the upper cover is clamped on the dish body, a plurality of dividing grooves are formed in the dish body, dividing dents are formed in the middle of the dividing grooves, the cross sections of the bottoms of the dividing dents are triangular, the top corners of the triangles are positioned on the central lines of the dividing grooves, two ends of the dividing dents are in contact with the upper end surfaces of the dividing grooves to form triangular openings, a plurality of fixing convex marks are formed in the dividing grooves and parallel to the dividing dents, a plurality of fixing teeth are uniformly distributed on the fixing convex marks and incline towards one end of the fixing convex marks.

Description

Animal embryo segmentation dish
Technical Field
The invention belongs to the technical field of segmentation dishes, and particularly relates to an animal embryo segmentation dish.
Background
When a fertilized egg develops into an embryo, the fertilized egg undergoes a process of dividing the fertilized egg into two, dividing the fertilized egg into four and dividing the fertilized egg into eight … …, and the embryo constituent cells in the early stage of the animal have totipotency, and theoretically each constituent cell of the early embryo has the capability of developing into a new individual, so that the early embryo of the animal is divided by using the modern embryo engineering technology, and each part of embryo formed by the division can develop into a new living individual, thereby greatly improving the breeding efficiency of the excellent animal, and simultaneously, the technology can simultaneously obtain an excellent animal population with consistent genetic properties. However, at present, the animal embryo segmentation lacks a special, effective and convenient operation device, and the animal embryo segmentation is mostly carried out by adopting a micro-operation system, which has the advantages of high price and high maintenance cost, and meanwhile, a skilled operation technician is required to carry out work. Therefore, a simple, convenient and low-cost special operating device is important for the effective segmentation of animal embryos.
Disclosure of Invention
The invention aims to provide an animal embryo segmentation vessel which can adapt to animal embryos with different diameters, an operator can cut the embryos by one hand, when the embryos are segmented, the fixed teeth can firmly fix the transparent belt on the periphery of the embryos, and the embryos are evenly and quickly cut by using an embryo segmentation knife.
In order to solve the technical problems, the invention adopts the following technical scheme:
the utility model provides an animal embryo cuts apart dish, including the disk body and upper cover, upper cover joint is on the disk body, be equipped with the several on the disk body and cut apart the recess, the intermediate position of cutting apart the recess is equipped with and cuts apart the dent, the bottom cross section of cutting apart the dent is triangle-shaped, triangle-shaped's apex angle is located the central line of cutting apart the recess, cut apart the dent both ends and cut apart the recess up end contact, form triangle-shaped's opening, be provided with a plurality of fixed protruding marks on cutting apart the recess on being on a parallel with cutting apart the dent, the equipartition has a plurality of fixed tooth on the fixed protruding mark, fixed tooth all inclines to fixed protruding mark one end, the volume of fixed tooth diminishes from fixed protruding mark one end to the other end gradually.
Furthermore, the fixed teeth are made of plastic and are integrally manufactured with the fixed convex marks.
The beneficial effects of the invention are as follows:
the design has fixed convex mark, and fixed tooth on the fixed convex mark fixes the embryo and conveniently fixes a position, because fixed tooth is to fixed convex mark one side slope, when splitting like this, fixed tooth just can hang embryo outlying zona pellucida, and the embryo is difficult for removing again, accomplishes embryo cutting.
Drawings
The invention will be described in further detail with reference to the drawings and the detailed description.
FIG. 1 is a schematic diagram of the present invention.
FIG. 2 is a schematic view of the dish body of the present invention.
FIG. 3 is a cross-sectional view of the dish body of the present invention.
FIG. 4 is a schematic view of a split groove according to the present invention.
Fig. 5 is a sectional view of a split groove of the present invention.
Detailed Description
Embodiments of the invention are described in detail below with reference to the attached drawings, but the invention can be implemented in a number of different ways, which are defined and covered by the claims.
As shown in the drawing, an animal embryo dividing dish comprises a dish body 1 and an upper cover 2, wherein the upper cover 2 is clamped on the dish body 1, a plurality of dividing grooves 10 are formed in the dish body 1, dividing dents 11 are formed in the middle of the dividing grooves 10, the cross section of the bottom of each dividing dent 11 is triangular, the vertex angle of the triangle is positioned on the central line of each dividing groove 10, so that embryos can be evenly divided into two parts, two ends of each dividing dent 11 are contacted with the upper end face of each dividing groove 10 to form a triangular opening 12, an operator can operate along the opening 12, the animal embryo dividing dish is quite convenient, a plurality of fixing dents 14 are uniformly distributed on each fixing dents 13 on each dividing groove 10 and are inclined towards one end of each fixing dents 13, the fixing dents 14 are made of plastic, the plastic materials are small in hardness and are integrally manufactured with the fixing dents 13, the embryos can be hooked by the aid of the fact that the embryos are not only damaged, the volumes of the fixing dents 14 are gradually reduced from one end of each fixing dents 13 to the other, and thus when the operator is in the operation, the volume of the animal embryo is remarkably reduced, namely, the fixed tooth 14 is positioned at the end of the animal embryo fixing dents 14, and the fixing effect is remarkably improved due to the fact that the fixing tooth 14 is positioned at one end of the fixing tooth is the end of the animal embryo.
In design, the upper opening of the dish body 1 can be designed into two specifications of 6cm multiplied by 6cm or 10cm multiplied by 10cm, and meanwhile, the dividing groove 10 is designed into a hemisphere with the diameter of 100-200 mu m, so that a single embryo can be placed, and a plurality of dividing grooves 10 are arranged on the dish body 1 in parallel in a whole row, so that a plurality of embryos can be divided simultaneously.
The design has fixed convex mark, and fixed tooth on the fixed convex mark fixes the embryo and conveniently fixes a position, because fixed tooth is to fixed convex mark one side slope, when splitting like this, fixed tooth just can hang embryo outlying zona pellucida, and the embryo is difficult for removing again, and fixed tooth material is right, accomplishes embryo cutting.
The embodiments of the invention described above are not intended to limit the scope of the invention, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the invention are intended to be included within the scope of the following claims.

Claims (2)

1. The utility model provides an animal embryo cuts apart ware, a serial communication port, including the ware body and upper cover, upper cover joint is on the ware body, be equipped with the several on the ware body and cut apart the recess, the intermediate position of cutting apart the recess is equipped with and cuts apart the dent, the bottom cross section of cutting apart the dent is triangle-shaped, the apex angle of triangle-shaped is located the central line of cutting apart the recess, cut apart the dent both ends and cut apart the recess up end contact, form triangle-shaped's opening, be provided with a plurality of fixed protruding marks on cutting apart the recess on being on a parallel with cutting apart the dent, the equipartition has a plurality of fixed tooth on the fixed protruding mark, fixed tooth all inclines to fixed protruding mark one end, the volume of fixed tooth diminishes gradually from fixed protruding mark one end to the other end, the size of fixed tooth is enough little enough, the size of fixed tooth is enough can catch on the embryo again not to damage the size of embryo, cut apart the recess for 100 ~ 200 mu m diameter hemisphere.
2. The animal embryo separating dish of claim 1 wherein the stationary teeth are made of plastic and are integrally formed with the stationary protrusions.
CN201710240677.6A 2017-04-13 2017-04-13 Animal embryo segmentation dish Active CN106834100B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710240677.6A CN106834100B (en) 2017-04-13 2017-04-13 Animal embryo segmentation dish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710240677.6A CN106834100B (en) 2017-04-13 2017-04-13 Animal embryo segmentation dish

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Publication Number Publication Date
CN106834100A CN106834100A (en) 2017-06-13
CN106834100B true CN106834100B (en) 2023-09-29

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5154188A (en) * 1991-05-09 1992-10-13 The Wolf Foundation Method and device for dividing embryos
JPH10244170A (en) * 1997-03-04 1998-09-14 Nikon Corp Substrate for dividing fine matter and its production
US6448069B1 (en) * 2000-06-09 2002-09-10 Genx International Corp. Embryo-culturing apparatus and method
JP2012029686A (en) * 2010-06-30 2012-02-16 Dainippon Printing Co Ltd Method for producing embryo by in vitro culture, and method, device, and system for selecting embryo
CN203033887U (en) * 2013-01-11 2013-07-03 中南大学 Cutting tool of special capillary glass tube for gamete and embryo operation
CN203683554U (en) * 2014-02-24 2014-07-02 中国农业科学院特产研究所 Oocyte and embryo culture device
CN204981870U (en) * 2015-09-16 2016-01-20 上海科华检验医学产品有限公司 Pick up ovum ware
CN105349485A (en) * 2015-12-15 2016-02-24 广西大学 Improved method for cutting water buffalo blastula by bare hands and cutting fluid
CN206692645U (en) * 2017-04-13 2017-12-01 明光现代农业科技合作推广服务中心 A kind of animal embryo splits ware

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5154188A (en) * 1991-05-09 1992-10-13 The Wolf Foundation Method and device for dividing embryos
JPH10244170A (en) * 1997-03-04 1998-09-14 Nikon Corp Substrate for dividing fine matter and its production
US6448069B1 (en) * 2000-06-09 2002-09-10 Genx International Corp. Embryo-culturing apparatus and method
JP2012029686A (en) * 2010-06-30 2012-02-16 Dainippon Printing Co Ltd Method for producing embryo by in vitro culture, and method, device, and system for selecting embryo
CN203033887U (en) * 2013-01-11 2013-07-03 中南大学 Cutting tool of special capillary glass tube for gamete and embryo operation
CN203683554U (en) * 2014-02-24 2014-07-02 中国农业科学院特产研究所 Oocyte and embryo culture device
CN204981870U (en) * 2015-09-16 2016-01-20 上海科华检验医学产品有限公司 Pick up ovum ware
CN105349485A (en) * 2015-12-15 2016-02-24 广西大学 Improved method for cutting water buffalo blastula by bare hands and cutting fluid
CN206692645U (en) * 2017-04-13 2017-12-01 明光现代农业科技合作推广服务中心 A kind of animal embryo splits ware

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
马保华 等.生产条件下安哥拉山羊胚胎徒手分割研究.《中国兽医学报》.1998,第18卷(第1期),第1.4、3节. *

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