CN201162013Y - Large-scale sifting motion cultivation plate - Google Patents
Large-scale sifting motion cultivation plate Download PDFInfo
- Publication number
- CN201162013Y CN201162013Y CNU2007201738808U CN200720173880U CN201162013Y CN 201162013 Y CN201162013 Y CN 201162013Y CN U2007201738808 U CNU2007201738808 U CN U2007201738808U CN 200720173880 U CN200720173880 U CN 200720173880U CN 201162013 Y CN201162013 Y CN 201162013Y
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- Prior art keywords
- grid
- extensive screening
- culturing plate
- chassis
- row
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- Expired - Fee Related
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a large scale screening culture plate, which belongs to the field of test equipment. The large scale screening culture plate comprises a chassis whose bottom surface of the lateral wall is a rectangle and a cover body which is buckled with the chassis, wherein the outer side of the bottom surface of the chassis is provided with a positioning coordinate grid and a positioning mark. The utility model is characterized in that the large scale screening culture plate which is designed aims at a 96-pore plate and a 384-pore plate, and each unit grid is perfectly corresponding. The large scale screening culture plate is convenient for large-scale high-flux screening work, which has simple employment and convenient cleaning, and can be used in circulating.
Description
Technical field
The utility model relates to a kind of extensive screening and culturing plate, belongs to the test equipment field.
Background technology
China utility model patent ZL200420013683.6 discloses a kind of specifically labelled Tissue Culture Dish that has, and is included in to cooperate on the disk that has cylindrical wall symmetrical loam cake is installed, and elements of a fix grid is being set on the back side of disk bottom.This Tissue Culture Dish makes people when carrying out cell cultures, can be convenient, fast, exactly individual cells or clone cell are positioned operation or record or exchange.
The disclosed this culture dish of ZL200420013683.6 is because the bottom surface is circular, and its horizontal comprehensive coordinate axis is by the center of circle, so be centrosymmetric.Though therefore be provided with grid, also can only relative positioning.In addition, for 96 orifice plates and 384 orifice plates that high flux screening often uses, this circle culture dish can't be corresponding one by one.
We are in carrying out gene clone, genetic expression and protein interaction equimolecular biology and biology screening experiment process, use present cultivation vessel and carry out these experiments, efficient is very low, the operation is loaded down with trivial details, make mistakes easily, can not screen in a large number and a large amount of experimental implementation.Therefore we just dream up and relevant 96 holes or 384 well culture plates of automated analysis at present, can satisfy bacterium, cell clone, single biomolecules and single biological respinse etc. and screen in a large number, have improved working efficiency significantly and reduce cost.
The utility model content
The technical problems to be solved in the utility model provides a kind of culture plate that extensive high flux screening is used that is fit to, especially can with the use that matches of 96 holes or 384 orifice plates.
For achieving the above object, the utility model adopts following design:
Extensive screening and culturing plate is that orthogonal chassis and the lid that fastens are with it formed by the bottom surface with sidewall, is provided with elements of a fix grid and telltale mark outside the bottom surface on chassis.
Described elements of a fix grid is to the lines of outer lug or the lines that concave.
Described elements of a fix grid is by the rectilinear(-al) that is parallel to the adjacent dual-side in bottom surface respectively, and grid is 12 row, 8 row; Or 24 row, 16 row.Cell is a square in the described grid, and the foursquare length of side is 0.5 centimetre or 1 centimetre.Suitable mutually with the cell size of 96 orifice plates or 384 orifice plates respectively.
Described telltale mark is to outer lug or the numeral that concaves and/or alphabetical.
Describedly be decided to be coordinate grid and telltale mark is a coating, adopt nonabradable coating to be printed on the outside, bottom surface on chassis.
The material of described extensive screening and culturing plate employing high temperature high voltage resistant such as glass or plastics are made, and are convenient to repeatedly use after the sterilization, can be one-body molded by mould.
Using method of the present utility model is as follows:
(1) with LB medium preparation LB large scale culturing plate;
The bacterium liquid that (2) will screen is coated on the LB large scale culturing;
(3) be upside down in 37 ℃ of cultivations;
(4) with duplicating translator, the various colony lifts that are grown in LB large scale culturing plate in the LB large scale culturing plate of another preparation, are upside down in 37 ℃ of cultivations, shift by one or many and cultivate, reach the purpose that fast high-flux filters out the purpose bacterial strain.
The utility model has the advantages that: at 96 orifice plates or the design of 384 orifice plates, each cell is ideally corresponding, is convenient to extensive high flux screening work, uses simply, and it is convenient to clean, and can be recycled.
The invention will be further described below in conjunction with the drawings and specific embodiments; it is not limitation of the invention; according to prior art well known in the art; embodiments of the present invention are not limited to this; therefore all this areas of having done according to the disclosure of invention be equal to replacement, all belong to protection scope of the present invention.
Description of drawings
Fig. 1 is the utility model overall appearance figure.
Fig. 2 is two integral part structure iron of the utility model.
Fig. 3 is the utility model bottom surface elements of a fix grid and telltale mark synoptic diagram.
Fig. 4 is the utility model elements of a fix grid and telltale mark structure iron 1.
Fig. 5 is the utility model elements of a fix grid and telltale mark structure iron 2.
Embodiment
Embodiment 1:
Consult Fig. 1-shown in Figure 4, extensive screening and culturing plate is that orthogonal chassis 1 and the lid 2 that fastens are with it formed by the bottom surface that has sidewall, is provided with elements of a fix grid 11 and telltale mark 12 outside the bottom surface on chassis 1.Telltale mark is to the numeral of outer lug and alphabetical.
Elements of a fix grid 11 is the lines to outer lug by some rectilinear(-al)s that are parallel to long limit, bottom surface and minor face respectively, and grid is 12 row, 8 row, and cell is the square of 1 centimetre of the length of side.
Embodiment 2:
Consult shown in Figure 5ly, extensive screening and culturing plate is that orthogonal chassis and the lid that fastens are with it formed by the bottom surface that has sidewall, is provided with elements of a fix grid and telltale mark outside the bottom surface on chassis.Telltale mark is concave digital and alphabetical.
Elements of a fix grid is the lines that concave by some rectilinear(-al)s that are parallel to long limit, bottom surface and minor face respectively, and grid is 24 row, 16 row, and cell is the square of 0.5 centimetre of the length of side.
Claims (8)
1. extensive screening and culturing plate is characterized in that: by the bottom surface with sidewall is that orthogonal chassis and the lid that fastens are with it formed, and is provided with elements of a fix grid and telltale mark outside the bottom surface on chassis.
2. extensive screening and culturing plate according to claim 1 is characterized in that: described elements of a fix grid is to the lines of outer lug or the lines that concave.
3. extensive screening and culturing plate according to claim 1 is characterized in that: described elements of a fix grid is by the rectilinear(-al) that is parallel to the adjacent dual-side in bottom surface respectively.
4. extensive screening and culturing plate according to claim 1 is characterized in that: described grid is 12 row, 8 row or 24 row, 16 row.
5. according to claim 3 or 4 described extensive screening and culturing plates, it is characterized in that: cell is square in the described grid, and the foursquare length of side is 0.5 centimetre or 1 centimetre.
6. extensive screening and culturing plate according to claim 1 is characterized in that: described telltale mark is to outer lug or the numeral that concaves and/or alphabetical.
7. extensive screening and culturing plate according to claim 1 is characterized in that: describedly be decided to be coordinate grid and telltale mark is a coating, adopt nonabradable coating to be printed on the outside, bottom surface on chassis.
8. extensive screening and culturing plate according to claim 1 is characterized in that: described extensive screening and culturing plate adopts the material of high temperature high voltage resistant to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007201738808U CN201162013Y (en) | 2007-10-30 | 2007-10-30 | Large-scale sifting motion cultivation plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007201738808U CN201162013Y (en) | 2007-10-30 | 2007-10-30 | Large-scale sifting motion cultivation plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201162013Y true CN201162013Y (en) | 2008-12-10 |
Family
ID=40182954
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007201738808U Expired - Fee Related CN201162013Y (en) | 2007-10-30 | 2007-10-30 | Large-scale sifting motion cultivation plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201162013Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012126328A1 (en) * | 2011-03-15 | 2012-09-27 | 新奥科技发展有限公司 | Lighting device, high throughput cultivation equipment comprising same |
| CN105838605A (en) * | 2016-06-06 | 2016-08-10 | 唐明 | Cell culture dish and cell sampling method thereof |
-
2007
- 2007-10-30 CN CNU2007201738808U patent/CN201162013Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012126328A1 (en) * | 2011-03-15 | 2012-09-27 | 新奥科技发展有限公司 | Lighting device, high throughput cultivation equipment comprising same |
| CN105838605A (en) * | 2016-06-06 | 2016-08-10 | 唐明 | Cell culture dish and cell sampling method thereof |
| CN105838605B (en) * | 2016-06-06 | 2018-05-11 | 唐明 | Tissue Culture Dish and its cell sampling method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: BEIJING BIOKIT, INC. Free format text: FORMER OWNER: CHEN YUBAO Effective date: 20101116 Free format text: FORMER OWNER: BEIJING SINOGREEN INSTITUTE FOR BIOECONOMY |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 100012 NO.6, DAYANGFANG, ANDINGMEN OUTSIDE, CHAOYANG DISTRICT, BEIJING TO: 100094 4/F, TOWER B, INCUBATION BUILDING, NO.7, FENGXIAN MIDDLE ROAD, YONGFENG INDUSTRIAL BASE, HAIDIAN DISTRICT, BEIJING |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20101116 Address after: 100094, Beijing, Yongfeng Haidian District industrial base, Feng Yin Road, No. 7 incubator building B, 4 floor Patentee after: Beijing Biokit Inc. Address before: 100012 Beijing, Chaoyang District Anwai Dayangfang No. 6 Co-patentee before: Beijing Zhongya economic Biological Research Institute Patentee before: Chen Yubao |
|
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081210 Termination date: 20121030 |