CN217536029U - Microporous plate capable of carrying out long-term administration culture on cells - Google Patents
Microporous plate capable of carrying out long-term administration culture on cells Download PDFInfo
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- CN217536029U CN217536029U CN202221480462.4U CN202221480462U CN217536029U CN 217536029 U CN217536029 U CN 217536029U CN 202221480462 U CN202221480462 U CN 202221480462U CN 217536029 U CN217536029 U CN 217536029U
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Abstract
The utility model discloses a can carry out the micropore board of culture of dosing for a long time to the cell relates to cell culture micropore board technical field, for solving current cell micropore board and changing culture solution process complex operation, and the cell loses easily, is unfavorable for the user to change the problem of culture solution. A culture hole layer is arranged in the middle of the upper surface of the flat-bottom microporous plate body; further comprising: the cell culture hole is arranged inside the culture hole layer and is of an integrated structure with the culture hole layer; the division board, it sets up the inside intermediate position department of cell culture hole, and it with cell culture hole block, the both sides of division board all are provided with the separating groove, the outer wall of division board is provided with the aperture, and the aperture size is 0.4-70um.
Description
Technical Field
The utility model relates to a cell culture micropore board technical field specifically is a micropore board that can carry out the cultivation of dosing for a long time to the cell.
Background
The micropore plate is a common consumable in biological experiments, is used for culturing cells in small quantity and analyzing, and is widely used for experiments such as drug screening. The current microwell plates are designed according to ANISILAS 4-2004 (R2012). The shape of the bottom can be divided into a flat bottom, a round bottom (U-shaped bottom), a pointed bottom (V-shaped bottom) and the like, and the area of the micropore can be divided into a whole area and a half area and the like. In culturing suspension cells, round-bottomed or sharp-bottomed culture plates are generally used. When cytological experiments are performed, sometimes the culture solution needs to be replaced, and for a large amount of cultured suspension cells, the culture solution can be replaced by centrifugation and other modes; however, in the case of suspension cells cultured in a micro-scale, for example, high-throughput compound screening is performed because the number of cells is small, and the replacement of the culture solution by centrifugation or the like is not only cumbersome, but also the cells are easily lost, so that it is difficult to replace the culture solution;
as in application No.: CN201821256106.8, the name is "a multi-functional micropore board" includes the mainboard, dustproof subassembly and sign subassembly, the sign subassembly includes the identification plate, slider and two rotation unit, rotation unit includes the label paper, the linkage unit, sliding ring and dwang, dustproof subassembly includes the slide bar, the slide, the drag hook, the arm-tie, two slip units and a plurality of dustproof units, dustproof unit includes montant and dustproof piece, this multi-functional micropore board passes through the sign subassembly and can carries out the sign to the position of cultivateing the hole on the mainboard, make things convenient for the experimenter to fix a position the cultivation hole fast, it is more convenient to make equipment use, moreover, when the micropore board restriction, make dustproof piece insert in cultivateing the hole through dustproof subassembly, carry out sealing process to the cultivation hole, prevent that the cultivation hole from receiving dust pollution, influence cleanness and cell culture, prevent dust through preventing to the cultivation hole, the practicality of this micropore board has been improved.
However, the operation of the existing cell microplate in the culture solution replacement process is complicated, and cells are easy to lose, which is not beneficial for a user to replace the culture solution; therefore, the existing demand is not satisfied, and a microplate capable of performing long-term administration culture on cells is proposed.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a can carry out the micropore board of the cultivation of dosing for a long time to the cell to solve the current cell micropore board that proposes in the above-mentioned background and change culture solution process complex operation, and the cell loses easily, is unfavorable for the user to change the problem of culture solution.
In order to achieve the above object, the utility model provides a following technical scheme: a microplate capable of performing long-term drug-delivery culture on cells, comprising: the culture hole layer is arranged in the middle of the upper surface of the flat-bottom microporous plate body;
further comprising:
the cell culture hole is arranged inside the culture hole layer and is of an integrated structure with the culture hole layer;
the division board, it sets up the inside intermediate position department of cell culture hole, and it with cell culture hole block, the both sides of division board all are provided with the separating groove, the outer wall of division board is provided with the aperture, and the aperture size is 0.4-70um.
Preferably, the outer wall of cell culture hole upper surface is provided with cultivates the hole notch, and cultivates hole notch and cell culture hole formula structure as an organic whole.
Preferably, the cell culture wells are arranged in a 12 × 8 horizontal direction.
Preferably, the separator is made of one of polycarbonate, polyester, and polyethylene terephthalate (pet) polytetrafluoroethylene.
Preferably, all be provided with the lid socket around the cultivation porose layer, and lid socket and flat base micropore board body formula structure as an organic whole.
Preferably, the inside of lid socket is provided with the lid slot, and lid slot and lid socket formula structure as an organic whole.
Compared with the prior art, the beneficial effects of the utility model are that:
the utility model discloses a set up cell culture hole and division board, can operate suspension cell's cultivation effectively, change the culture medium, perhaps add compound, the concentration of adjustment compound etc. because the existence of middle division board, can effectively prevent to trade the influence of liquid operation to the cell to can carry out the culture of dosing for a long time to the cell, even if change liquid many times repeatedly, also smaller to the influence of cell, be favorable to guaranteeing the quality of cytology experiment.
Drawings
Fig. 1 is an overall plan view of the present invention;
fig. 2 is a left side view of the present invention;
FIG. 3 is a schematic view of the structure of the cell culture well according to the present invention;
in the figure: 1. a flat-bottom microporous plate body; 2. a cover body inserting opening; 3. culturing a pore layer; 4. a cell culture well; 5. culturing Kong Caokou; 6. a partition plate; 7. a separation tank; 8. a cover body slot.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
Referring to fig. 1-3, the present invention provides an embodiment: a microplate capable of performing long-term drug-delivery culture on cells, comprising: the culture hole layer 3 is arranged in the middle of the upper surface of the flat-bottom microporous plate body 1;
further comprising:
a cell culture hole 4 which is provided inside the culture hole layer 3 and has an integral structure with the culture hole layer 3;
the division plate 6 is arranged in the middle of the cell culture hole 4 and is clamped with the cell culture hole 4, the two sides of the division plate 6 are provided with the division grooves 7, the outer wall of the division plate 6 is provided with small holes, the size of each small hole is 0.4-70um, and the small holes have the effect that cells can permeate small-molecule medicines or cytokines.
Referring to the figure, the outer wall of the upper surface of the cell culture hole 4 is provided with a culture hole notch 5, the culture hole notch 5 and the cell culture hole 4 are of an integrated structure, and the culture hole notch 5 has an effect of facilitating a user to place required cells and culture solution.
Referring to the figure, the cell culture wells 4 are arranged in a 12 × 8 horizontal direction, so that the number of the cell culture wells 4 is increased, and a user can culture a plurality of cells at one time.
Referring to the figure, the material of the partition plate 6 is one of polycarbonate, polyester and polyethylene terephthalate, and the partition plate 6 made of the material is more difficult to damage, so that the service life is prolonged.
Please refer to the figure, the periphery of the culture hole layer 3 is provided with the cover body socket 2, and the cover body socket 2 and the flat-bottom microporous plate body 1 are of an integrated structure, and the cover body socket 2 has the effect of being conveniently combined with the cover body.
Referring to the drawings, a cover slot 8 is disposed inside the cover socket 2, the cover slot 8 and the cover socket 2 are integrated, and the cover slot 8 has an effect of inserting the cover.
The working principle is as follows: the partition plate 6 is arranged in the middle position inside the cell culture hole 4, two sides of the partition plate are divided into partition grooves 7, when the culture medium for suspending cells needs to be replaced, the cells can be inoculated on one side of the cell culture hole 4, and the other side is provided with the equal volume of the culture medium; when the liquid needs to be changed, the culture medium on the cell-free side is directly sucked out, and the culture medium needing to be changed or the culture medium containing the compound is added.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (6)
1. A microporous plate capable of carrying out long-term administration culture on cells comprises a flat-bottom microporous plate body (1), wherein a culture hole layer (3) is arranged in the middle position of the upper surface of the flat-bottom microporous plate body (1);
the method is characterized in that: further comprising:
a cell culture hole (4) which is arranged inside the culture hole layer (3) and is of an integrated structure with the culture hole layer (3);
division board (6), its setting is in the inside intermediate position department of cell culture hole (4), and it with cell culture hole (4) block, the both sides of division board (6) all are provided with separating groove (7), the outer wall of division board (6) is provided with the aperture, and the aperture size is 0.4-70um.
2. The microplate for the long-term administration and culture of cells according to claim 1, wherein: the outer wall of the upper surface of the cell culture hole (4) is provided with a culture Kong Caokou (5), and the culture Kong Caokou (5) and the cell culture hole (4) are of an integrated structure.
3. The microplate for the long-term administration and culture of cells according to claim 1, wherein: the arrangement mode of the cell culture holes (4) is 12 multiplied by 8 in the transverse and vertical directions.
4. The microplate for the long-term administration and culture of cells according to claim 1, wherein: the partition plate (6) is made of one of polycarbonate, polyester, polyethylene terephthalate or polytetrafluoroethylene.
5. The microplate for the long-term administration and culture of cells according to claim 1, wherein: cultivate all being provided with lid socket (2) around porose layer (3), and lid socket (2) and flat micropore board body (1) formula structure as an organic whole.
6. The microplate for the long-term administration and culture of cells according to claim 5, wherein: the inside of lid socket (2) is provided with lid slot (8), and lid slot (8) and lid socket (2) formula structure as an organic whole.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202221480462.4U CN217536029U (en) | 2022-06-14 | 2022-06-14 | Microporous plate capable of carrying out long-term administration culture on cells |
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CN202221480462.4U CN217536029U (en) | 2022-06-14 | 2022-06-14 | Microporous plate capable of carrying out long-term administration culture on cells |
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CN217536029U true CN217536029U (en) | 2022-10-04 |
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CN202221480462.4U Active CN217536029U (en) | 2022-06-14 | 2022-06-14 | Microporous plate capable of carrying out long-term administration culture on cells |
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2022
- 2022-06-14 CN CN202221480462.4U patent/CN217536029U/en active Active
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