CN214781898U

CN214781898U - Six-hole plate for cell culture

Info

Publication number: CN214781898U
Application number: CN202120425719.5U
Authority: CN
Inventors: 杨梅; 邹欢; 黄艳明
Original assignee: Second Affiliated Hospital Army Medical University
Current assignee: Second Affiliated Hospital Army Medical University
Priority date: 2021-02-26
Filing date: 2021-02-26
Publication date: 2021-11-19
Anticipated expiration: 2031-02-26

Abstract

The utility model discloses a six-hole plate for cell culture, which comprises a plate body and a culture cover; six culture holes are arranged on the plate body, and the culture cover is sleeved at the top of the plate body; all be provided with isolating mechanism on the lateral wall of two adjacent cultivation holes, isolating mechanism includes that two stands and detachable set up the dodge gate between two stands, forms the intercommunication notch that communicates two adjacent cultivation holes between two stands that correspond. The isolation mechanism is arranged, so that when a single culture hole is needed to be used independently for culturing cells, the isolation door of the isolation mechanism is clamped on the two corresponding upright posts; when the adjacent culture holes need to be communicated, the isolation door is taken down; the U-shaped sealing strips are arranged on the two side walls of the inverted U-shaped door body, so that two seals are formed between the adjacent culture holes, and the culture solution between the adjacent culture holes can be favorably separated.

Description

Six-hole plate for cell culture

Technical Field

The utility model relates to a biotechnology experiment instrument field, concretely relates to six orifice plates for cell culture.

Background

At present, a six-hole cell culture plate is a vessel frequently used in the biochemical related field for culturing cells, and six independent holes for culturing cells are arranged on the six-hole cell culture plate and used for culturing the cells. In the process of culturing cells, in order to observe the migration of a cell, one or more straight lines (cell scratching experiments) need to be scratched at the bottom of a culture hole full of cells by a foreign object, the migration capability of the cell is determined by observing the expansion range of the cell in the growth process to the scratched straight line area, the scratching process is easy to cause the pollution of the cell, and different scratching tools, force and methods are different in the size and the curve of the scratched straight line, so that the error of the experiment is caused, the repeatability of the experiment is influenced, and the experiment is even failed. However, the growth of cells at the junction of two or more cells in co-culture is difficult to be observed in the prior art, and a simple culture device convenient for observation is urgently needed to complete the independent culture and then mixed culture of the cells, which is beneficial to better research on the interaction between the cells.

SUMMERY OF THE UTILITY MODEL

In view of the above, the present invention provides a six-hole plate for cell culture, which is provided with an isolation mechanism, wherein when a single culture hole is needed to be used alone for culturing cells, an isolation door of the isolation mechanism is clamped on two corresponding columns; when the adjacent culture holes need to be communicated, the isolation door is taken down.

The utility model discloses a following technical scheme realizes:

a six-well plate for cell culture, comprising a plate body and a culture cover; six culture holes are formed in the plate body, and the culture cover is sleeved at the top of the plate body; two adjacent all be provided with isolation mechanism on the lateral wall of cultivateing the hole, isolation mechanism includes that two stands and detachable set up two isolation door between the stand, two correspond form between the stand and communicate two adjacent the intercommunication notch of cultivateing the hole.

Further, the isolation door comprises an inverted U-shaped door body clamped between the two upright posts and two U-shaped sealing strips arranged on two side walls of the U-shaped door body; the inverted U-shaped door body is made of elastic materials.

Further, six culture holes are distributed on the plate body in an array.

Further, the tops of the six culture wells are 5mm higher than the top of the plate body; the outer fringe at the top of the plate body is provided with the manger plate sand grip, the height of manger plate sand grip is 3 mm.

Furthermore, an isolation filter screen is arranged between the two corresponding upright columns; and the corresponding two upright columns are provided with U-shaped slots which are convenient for inserting the isolation filter screen.

Further, the isolation filter screen comprises an isolation frame and a filter screen arranged in the isolation frame.

The beneficial effects of the utility model reside in that: through setting up isolation mechanism, when needs single cultivation hole of exclusive use cultivates the cell, with isolation mechanism's isolation door joint on two stands that correspond, set up the U-shaped sealing strip on two lateral walls of the U-shaped door body of falling for it is sealed that to form twice between the adjacent cultivation hole, be favorable to cutting off the cultivation liquid between the adjacent cultivation hole, reach different cell separately cultivates, and can communicate adjacent cultivation hole again after taking off the isolation door, reach the mixed culture of cell.

Drawings

Fig. 1 is the schematic diagram of the internal structure of the present invention:

FIG. 2 is a schematic view of the structure A-A of FIG. 1;

FIG. 3 is an enlarged view of the structure at C in FIG. 2;

FIG. 4 is a schematic structural view of a plate body provided with six culture holes;

FIG. 5 is a schematic structural diagram of an isolation gate;

FIG. 6 is a schematic view of the structure B-B of FIG. 5.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the above description of the present invention, it should be noted that the terms "one side" and "the other side" are used for indicating the position or the positional relationship based on the position or the positional relationship shown in the drawings, or the position or the positional relationship which is usually placed when the product of the present invention is used, only for the convenience of describing the present invention and simplifying the description, but not for indicating or implying that the indicated device or element must have a specific position, be constructed and operated in a specific position, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.

Further, the term "identical" and the like do not mean that the components are absolutely required to be identical, but may have slight differences. The term "perpendicular" merely means that the positional relationship between the components is more perpendicular than "parallel", and does not mean that the structure must be perfectly perpendicular, but may be slightly inclined.

As shown in fig. 1 to 6, a six-well plate for cell culture comprises a plate body 1 and a culture lid 2; six culture holes 3 are formed in the plate body 1, and the culture cover 2 is sleeved on the top of the plate body 1; the side walls of two adjacent culture holes 3 are respectively provided with an isolation mechanism 4, each isolation mechanism 4 comprises two upright posts 5 and an isolation door 6 which is detachably arranged between the two upright posts 5, and a communication notch for communicating the two adjacent culture holes 3 is formed between the two corresponding upright posts 5; the outer sides of the two upright posts 5 are connected with the side walls of the two corresponding culture holes 3; by arranging the isolation mechanism, when cells need to be cultured by using a single culture hole independently, the isolation door of the isolation mechanism is clamped on the two corresponding upright posts; when the adjacent culture holes need to be communicated, the isolation door is taken down.

Further, the isolation door 6 comprises an inverted U-shaped door body 61 clamped between the two upright posts 5 and two U-shaped sealing strips 62 arranged on two side walls of the U-shaped door 61; the U-shaped sealing strips are adhered to the lower end of the side wall of the corresponding U-shaped door body and the two ends of the inner wall of the side wall; during assembly, the inverted U-shaped door body is clamped on the two corresponding stand columns, and the U-shaped sealing strips form sealing between the inverted U-shaped door body and the bottom wall of the culture hole and between the inverted U-shaped door body and the two corresponding stand columns, so that the culture solution mixing between the adjacent culture holes is avoided; the U-shaped sealing strips are arranged on the two side walls of the inverted U-shaped door body, so that two seals are formed between the adjacent culture holes, and the culture solution between the adjacent culture holes can be favorably separated; the inverted U-shaped door body is made of elastic materials, so that the isolating door can be clamped on the two corresponding stand columns, and the sealing reliability of the isolating door is improved.

Furthermore, six culture wells 3 are distributed on the plate body 1 in an array.

Further, the tops of the six culture holes 3 are 5mm higher than the top of the plate body 1; the outer edge at the top of the plate body 1 is provided with a water retaining convex strip, and the height of the water retaining convex strip is 3 mm.

Furthermore, an isolation filter screen 7 is arranged between the two corresponding upright columns 5; the A and B cells in two adjacent culture holes can produce some substances such as hormone and growth factor by themselves during growth, and also can metabolize some substances to be discharged out of the cells, and the production of different cells is different; when the isolating door is opened, the liquid in the two culture holes flows mutually, and A and B cell bodies do not contact (isolated by an isolating filter screen), so that substances generated in the growth process of the liquid and the B cell bodies flow into the other culture hole, and the influence of the substances generated by the A cell on the B cell or the influence of the substances generated by the B cell on the A cell can be observed conveniently; and the corresponding two upright columns 5 are provided with U-shaped slots which are convenient for inserting the isolation filter screens 7.

Further, the isolation screen 7 includes an isolation frame 71 and a screen 72 disposed in the isolation frame 71.

Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.

Claims

1. A six-well plate for cell culture, comprising a plate body and a culture cover; six culture holes are formed in the plate body, and the culture cover is sleeved at the top of the plate body; the method is characterized in that: two adjacent all be provided with isolation mechanism on the lateral wall of cultivateing the hole, isolation mechanism includes that two stands and detachable set up two isolation door between the stand, two correspond form between the stand and communicate two adjacent the intercommunication notch of cultivateing the hole.

2. A six-well plate for cell culture according to claim 1, wherein: the isolation door comprises an inverted U-shaped door body clamped between the two upright posts and two U-shaped sealing strips arranged on two side walls of the U-shaped door body; the inverted U-shaped door body is made of elastic materials.

3. A six-well plate for cell culture according to claim 1, wherein: six culture holes are distributed on the plate body in an array.

4. A six-well plate for cell culture according to claim 1, wherein: the tops of the six culture holes are 5mm higher than the top of the plate body; the outer fringe at the top of the plate body is provided with the manger plate sand grip, the height of manger plate sand grip is 3 mm.

5. A six-well plate for cell culture according to claim 1, wherein: an isolation filter screen is arranged between the two corresponding upright columns; and the corresponding two upright columns are provided with U-shaped slots which are convenient for inserting the isolation filter screen.

6. A six-well plate for cell culture according to claim 5, wherein: keep apart including the isolation frame of filter screen and set up the filter screen in the isolation frame.