CN214529075U - Cell culture dish - Google Patents
Cell culture dish Download PDFInfo
- Publication number
- CN214529075U CN214529075U CN202120425710.4U CN202120425710U CN214529075U CN 214529075 U CN214529075 U CN 214529075U CN 202120425710 U CN202120425710 U CN 202120425710U CN 214529075 U CN214529075 U CN 214529075U
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- CN
- China
- Prior art keywords
- isolation
- dish
- cell culture
- dish body
- frame
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000004113 cell culture Methods 0.000 title claims abstract description 16
- 238000002955 isolation Methods 0.000 claims abstract description 86
- 238000004891 communication Methods 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 238000010276 construction Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 16
- 239000000126 substance Substances 0.000 description 5
- 210000003719 b-lymphocyte Anatomy 0.000 description 3
- 238000003501 co-culture Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture dish, which comprises a cylindrical dish body and a dish cover sleeved on the top of the dish body; the inner cavity of the dish body is provided with an isolation frame which divides the inner cavity of the dish body into a plurality of independent areas, and the isolation frame is provided with a plurality of isolation structures which can be communicated with adjacent areas; the isolation frame is provided with communication holes corresponding to the isolation structures, and the communication holes are convenient for communicating the two sides of the isolation frame; the isolation structure comprises an isolation door and an isolation filter screen; the isolation doors and the isolation filter screens are arranged in a one-to-one correspondence manner; the middle part of the outer wall of the dish body is provided with a limit ring for limiting the position of the dish cover. The utility model is provided with the isolation frame, which is convenient for dividing the inner cavity of the dish body into a plurality of independent areas; through set up a plurality of isolating construction on the isolation frame, be convenient for as required direct intercommunication adjacent region or through keeping apart the adjacent region of filter screen intercommunication.
Description
Technical Field
The utility model relates to a biotechnology experimental tools field, concretely relates to cell culture dish.
Background
The in vitro cell culture technology is a technical method widely adopted by various subject researches such as cytology, genetics, oncology, pharmacology and the like at present; cell culture is a method in which living tissues or cells are taken out of a test animal/plant body and placed in an in vitro environment simulating an in vivo living environment, so that the cells continue to grow.
The existing culture dish for culturing cells only has one inner cavity, only one culture solution can be used for culturing one cell at a time, and the common culture of different cells on the same plane is difficult to realize; the living animal/plant is composed of a plurality of cells, and the growth change and the function change of the cells observed under the condition of the co-culture of the plurality of cells are close to the normal in-vivo environment of organisms compared with the condition of single cell culture. The culture dish capable of flexibly and conveniently segmenting and fusing various cells provides better help for mixing culture solutions of different cells to form a multi-cell co-culture system and dynamically observing growth change, function change and the like of the cells under the co-culture condition.
SUMMERY OF THE UTILITY MODEL
In view of the above, the present invention provides a cell culture dish, which is provided with an isolation frame to facilitate dividing an inner cavity of a dish body into a plurality of independent areas; through set up a plurality of isolating construction on the isolation frame, be convenient for as required direct intercommunication adjacent region or through keeping apart the adjacent region of filter screen intercommunication.
The utility model discloses a following technical scheme realizes:
a cell culture dish comprises a cylindrical dish body and a dish cover sleeved at the top of the dish body; the inner cavity of the dish body is provided with an isolation frame which divides the inner cavity of the dish body into a plurality of independent areas, and the isolation frame is provided with a plurality of isolation structures which can be communicated with adjacent areas; the isolation frame is provided with communication holes corresponding to the isolation structures, and the communication holes are convenient for communicating the two sides of the isolation frame; the isolation structure comprises an isolation door and an isolation filter screen; the isolation doors and the isolation filter screens are arranged in a one-to-one correspondence manner; and a limiting ring for limiting the position of the dish cover is arranged in the middle of the outer wall of the dish body.
Further, the isolation frame and the dish body are integrally arranged; the isolation frame comprises two transverse plates and two vertical plates which are integrally arranged.
Furthermore, two sides of the vertical plate and the transverse plate corresponding to the isolation structure are respectively provided with a connecting structure which is convenient for inserting the isolation door/the isolation filter screen; the connecting structure comprises two L-shaped plates, and the upper ends of the two L-shaped plates are provided with limiting strips which are convenient for limiting the isolating door/isolating filter screen.
Furthermore, the upper ends of the two L-shaped plates are provided with jacks which are convenient for inserting the limiting strips.
Furthermore, the periphery of the isolating door is provided with a first annular groove which is convenient for sleeving the first sealing ring.
Further, the isolation filter screen comprises an isolation frame and a filter screen arranged in the isolation frame; the periphery of the isolation frame is provided with a second annular groove which is convenient for sleeving a second sealing ring.
Furthermore, a plurality of circular arc-shaped ventilation grooves are uniformly formed in the outer wall of the limiting ring.
The beneficial effects of the utility model reside in that: the separation frame is arranged, so that the inner cavity of the dish body is conveniently divided into a plurality of independent areas; through set up a plurality of isolating construction on the isolation frame, be convenient for as required direct intercommunication adjacent region or through keeping apart the adjacent region of filter screen intercommunication.
Drawings
Fig. 1 is the schematic diagram of the internal structure of the present invention:
FIG. 2 is an enlarged schematic view of the structure at A in FIG. 1;
FIG. 3 is an enlarged view of the structure at B in FIG. 1;
figure 4 is a top view of the capsule after assembly of the isolation structure.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the above description of the present invention, it should be noted that the terms "one side" and "the other side" are used for indicating the position or the positional relationship based on the position or the positional relationship shown in the drawings, or the position or the positional relationship which is usually placed when the product of the present invention is used, only for the convenience of describing the present invention and simplifying the description, but not for indicating or implying that the indicated device or element must have a specific position, be constructed and operated in a specific position, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.
Further, the term "identical" and the like do not mean that the components are absolutely required to be identical, but may have slight differences. The term "perpendicular" merely means that the positional relationship between the components is more perpendicular than "parallel", and does not mean that the structure must be perfectly perpendicular, but may be slightly inclined.
As shown in fig. 1 to 4, a cell culture dish comprises a cylindrical dish body 1 and a dish cover 2 sleeved on the top of the dish body; the dish body and the dish cover are made of transparent materials, the inner cavity of the dish body 1 is provided with an isolation frame 3 which divides the inner cavity of the dish body 1 into a plurality of independent areas, and the isolation frame 3 is provided with a plurality of isolation structures 4 which can be communicated with adjacent areas; the isolation frame is provided with communication holes corresponding to the isolation structures 4, and the communication holes are convenient for communicating the two sides of the isolation frame 3; after the isolating door is convenient to disassemble, the culture solution on the two sides is mixed; the isolation structure 4 comprises an isolation door 41 and an isolation filter screen 42; the cells A and B in two adjacent culture areas produce some substances such as hormone and growth factor by themselves during growth, and metabolize some substances to be discharged out of the cells, and the production of different cells is different; when the isolation door is opened, the liquid in the two culture areas flows mutually, and the A cell body and the B cell body do not contact (isolated by the isolation filter screen), so that substances generated in the growth process of the liquid flow into the other culture area, and the influence of the substances generated by the A cell on the B cell or the influence of the substances generated by the B cell on the A cell can be observed conveniently; the isolation doors 41 and the isolation filter screens 42 are arranged in a one-to-one correspondence manner; a limiting ring 11 for limiting the position of the dish cover 2 is arranged in the middle of the outer wall of the dish body 1; the position of the dish cover is convenient to limit, and a certain ventilation gap is formed between the inner wall of the dish cover and the isolation frame.
Further, the isolation frame 3 and the dish body 1 are integrally arranged; the isolation frame 3 comprises two transverse plates 31 and two vertical plates 32 which are integrally arranged.
Furthermore, two sides of the vertical plate 32 and the transverse plate 31 corresponding to the isolation structure 4 are provided with connecting structures for conveniently inserting the isolation door 41/the isolation filter screen 42; the connecting structure comprises two L-shaped plates 43, and a slot for inserting the isolation door 41/the isolation filter screen 42 is formed between each L-shaped plate 43 and the corresponding transverse plate 31/vertical plate 32; the upper ends of the two L-shaped plates are provided with limiting strips 44 which are convenient for limiting the isolation door 41/the isolation filter screen 42; the lower extreme in the middle part of spacing is provided with convex recess, convenient to detach spacing.
Further, the upper ends of the two L-shaped plates 43 are provided with insertion holes for inserting the stopper bars 44.
Further, a ring first-shaped groove for conveniently sleeving the first sealing ring 45 is formed on the periphery of the isolation door 41.
Further, the isolation screen 42 comprises an isolation frame 421 and a screen 422 arranged in the isolation frame 421; the periphery of the isolation frame 421 is provided with a second annular groove for conveniently sleeving the second sealing ring 46.
Further, a plurality of circular arc-shaped ventilation grooves are uniformly formed in the outer wall of the limiting ring 11; the inner area of the dish body is communicated with the outer part of the dish body through the ventilating groove and the gap between the dish cover and the dish body.
Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.
Claims (7)
1. A cell culture dish comprises a cylindrical dish body and a dish cover sleeved at the top of the dish body; the method is characterized in that: the inner cavity of the dish body is provided with an isolation frame which divides the inner cavity of the dish body into a plurality of independent areas, and the isolation frame is provided with a plurality of isolation structures which can be communicated with adjacent areas; the isolation frame is provided with communication holes corresponding to the isolation structures, and the communication holes are convenient for communicating the two sides of the isolation frame; the isolation structure comprises an isolation door and an isolation filter screen; the isolation doors and the isolation filter screens are arranged in a one-to-one correspondence manner; and a limiting ring for limiting the position of the dish cover is arranged in the middle of the outer wall of the dish body.
2. A cell culture dish according to claim 1, wherein: the isolation frame and the dish body are integrally arranged; the isolation frame comprises two transverse plates and two vertical plates which are integrally arranged.
3. A cell culture dish according to claim 2, wherein: the vertical plate and the transverse plate are provided with connecting structures corresponding to the two sides of the isolation structure, so that the isolation door/the isolation filter screen can be conveniently inserted into the connecting structures; the connecting structure comprises two L-shaped plates, and the upper ends of the two L-shaped plates are provided with limiting strips which are convenient for limiting the isolating door/isolating filter screen.
4. A cell culture dish according to claim 3, wherein: and the upper ends of the two L-shaped plates are provided with jacks which are convenient for inserting the limiting strips.
5. A cell culture dish according to claim 1, wherein: the periphery of the isolation door is provided with a first annular groove which is convenient for sleeving a first sealing ring.
6. A cell culture dish according to claim 1, wherein: the isolation filter screen comprises an isolation frame and a filter screen arranged in the isolation frame; the periphery of the isolation frame is provided with a second annular groove which is convenient for sleeving a second sealing ring.
7. A cell culture dish according to claim 1, wherein: the outer wall of the limiting ring is evenly provided with a plurality of circular arc-shaped ventilation grooves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120425710.4U CN214529075U (en) | 2021-02-26 | 2021-02-26 | Cell culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120425710.4U CN214529075U (en) | 2021-02-26 | 2021-02-26 | Cell culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214529075U true CN214529075U (en) | 2021-10-29 |
Family
ID=78258736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120425710.4U Expired - Fee Related CN214529075U (en) | 2021-02-26 | 2021-02-26 | Cell culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214529075U (en) |
-
2021
- 2021-02-26 CN CN202120425710.4U patent/CN214529075U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211029 |