CN209696867U - High-throughput hydrogel original position molding machine - Google Patents
High-throughput hydrogel original position molding machine Download PDFInfo
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- CN209696867U CN209696867U CN201821547880.4U CN201821547880U CN209696867U CN 209696867 U CN209696867 U CN 209696867U CN 201821547880 U CN201821547880 U CN 201821547880U CN 209696867 U CN209696867 U CN 209696867U
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- microlayer model
- original position
- molding machine
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Abstract
The utility model discloses a kind of high-throughput hydrogel original position molding machine, including casing ontology and crosslinking agent storage container, multi-channel high frequency concussion microlayer model generator and multichannel microlayer model conveyor module by being successively set under upper in the casing ontology；The multichannel microlayer model conveyor module includes microlayer model conveying cavity, the microwell plate for being set to microlayer model conveying cavity upper end and the multiple microlayer model transfer pipelines for being set to microlayer model conveying cavity lower end.The high-throughput hydrogel original position molding machine of the utility model generates crosslinking agent microlayer model by high frequency oscillation, and by designing there is the multichannel microlayer model conveyor module of microchannel microlayer model is evenly distributed to each hole of porous culture plate, realize that the high throughput of hydrogel is cross-linked in situ.The high-throughput hydrogel original position molding machine of the utility model is safe and reliable, efficiently convenient, can meet the needs of different field researcher's hydrogel high throughput formed in situ, meet the developing direction of biomaterial He cell biology.
Description
Technical field
The utility model relates to cell biology, organizational project and biomaterial and field of biotechnology, in particular to one
The high-throughput hydrogel original position molding machine of kind.
Background technique
It is known that three-dimensional cell cultivation technology is relative to traditional two dimension culture, there is many advantages, researchers
This conclusion is all confirmed from metabolism group, protein expression, nucleic acid level, but raw in organizational project, biomaterial and cell
The field of biotechnology such as object are often used the dimensional culture technology of cell, and biological study personnel are often due to lacking biological material
Expect that the knowledge of aspect is still studied using traditional two-dimentional cell culture model, or by means of letters such as hydrogels in the market
Single Three-dimensional cell culture product carries out, and not only expensive there is also many drawbacks.Common three-dimensional cell cultivation technology is pressed
According to whether there is or not brackets to be divided into no support rack type three-dimensional cell cultivation and support rack type three-dimensional cell cultivation, no bracket three-dimensional cell cultivation is divided into
Ultralow adhesion sheet culture, Hanging drop culture, rotary rolling culture and magnetization cultivation, and support rack type three-dimensional cell cultivation is according to bracket
The difference of type is divided into nano-screen three-dimensional cell cultivation and hydrogel three-dimensional cell cultivation.
Three-dimensional cell cultivation has extremely wide answer in stem-cell research, tumor research and drug discovery and development field
With prospect, as needed largely to test in drug screening research, the less dimensional culture of the dimensional culture of simplified manual and channel
Mode is increasingly unable to satisfy application demand, and three-dimensional cell cultivation technology is many kinds of, and wherein culture effect is best for water
The dimensional culture technology of gel form, but flux is lower complicated for operation and form of hydrogels dimensional culture is difficult to form automation.
Therefore, it is necessary to develop a kind of hydrogel original position molding machine of high throughput and method, which can be used as functional module
Integrated automation work station uses, and also can be used alone.
Utility model content
The technical problem to be solved by the utility model is in view of the deficiency of the prior art, provide a kind of high pass
Measure hydrogel original position molding machine.
In order to solve the above technical problems, the technical solution adopted in the utility model is: it is a kind of high throughput hydrogel original position at
Type device, including casing ontology and crosslinking agent storage container, multichannel by being successively set under upper in the casing ontology
High frequency oscillation microlayer model generator and multichannel microlayer model conveyor module；
The crosslinking agent storage container shakes the sealing of microlayer model generator by the multi-channel high frequency of buckle and its bottom and connects
It connects, the through-hole that crosslinking agent storage container bottom opens up is connected to the high frequency oscillation microlayer model generator upper end；
The multichannel microlayer model conveyor module includes microlayer model conveying cavity, is set on the microlayer model conveying cavity
The microwell plate at end and the multiple microlayer model transfer pipelines for being set to microlayer model conveying cavity lower end, the microwell plate upper end with
The high frequency oscillation microlayer model generator bottom connection, microlayer model transfer pipeline lower end and the porous culture plate upper end connect
It is logical.
Preferably, the casing ontology lower end is provided with for the fixed support multichannel microlayer model conveyor module
Bracket, the outer rim of the casing ontology lower end are additionally provided with porous culture plate cooperation frame.
Preferably, the multi-channel high frequency concussion microlayer model generator includes that high frequency oscillation microlayer model forms plate, opens up
The high frequency oscillation microlayer model formed plate on multiple concussion piece mounting grooves and be equipped in the concussion piece mounting groove
High frequency oscillation piece.
Preferably, offer what lower end was connected to the multichannel microlayer model conveyor module in the concussion piece mounting groove
Fluid hole.
Preferably, the microwell plate upper end is provided with connector, and the microwell plate is internally provided with microchannel, the company
Offered in connector upper end and the high frequency oscillation microlayer model formed on plate go out liquid be connected to and lower end and the microchannel into
The delivery hole of fluid apertures connection.
Preferably, the microchannel includes multiple liquid outlet microporess, and the multiple liquid outlet micropores and the microlayer model convey
Multiple microlayer model transfer pipelines of cavity lower end, which correspond, to be connected to, under the liquid outlet of the multiple microlayer model transfer pipeline and its
Multiple micropores on the porous culture plate at end correspond connection.
Preferably, gasket seal is additionally provided on the delivery hole.
Preferably, the casing ontology upper end is provided with the cover board slidably opened, and goes back on the casing ontology outer wall
It is provided with power knob, control circuit board and battery charging interface.
Preferably, rechargeable battery is additionally provided on the casing ontology.
The beneficial effects of the utility model are: the high-throughput hydrogel original position molding machine of the utility model is shaken by high frequency
It dissipates one's fortune raw crosslinking agent microlayer model, and has the multichannel microlayer model conveyor module of microchannel by microlayer model mean allocation by design
To each hole of porous culture plate, realize that the high throughput of hydrogel is cross-linked in situ.The high-throughput hydrogel original position of the utility model forms
Equipment safety is reliable, efficiently convenient, can meet the needs of different field researcher's hydrogel high throughput formed in situ, meet life
The developing direction of object materialogy and cell biology has good popularization and application foreground.
Detailed description of the invention
Fig. 1 is the external structure schematic diagram of the high-throughput hydrogel original position molding machine of the utility model；
Fig. 2 is the schematic diagram of internal structure of the high-throughput hydrogel original position molding machine of the utility model；
Fig. 3 is that the internal structure of the high-throughput hydrogel original position molding machine of the utility model is layered display diagram；
Fig. 4 is that the crosslinking agent of the utility model stores the cooperation schematic diagram of module and high frequency oscillation microlayer model generator；
Fig. 5 is the decomposition diagram of the microlayer model conveyor module of the utility model；
Fig. 6 is the structural schematic diagram of the microchannel of the utility model；
Fig. 7 is the structural schematic diagram of the bracket of the utility model；
Fig. 8 is that the porous culture plate of the utility model cooperates the structural schematic diagram of frame.
01 cover board；02 charging interface；03 power knob；04 porous culture plate；05 crosslinking agent storage container；06 chargeable electricity
Pond；07 multi-channel high frequency shakes microlayer model generator；08 control circuit board；09 multichannel microlayer model conveyor module；10 buckles；11
High frequency oscillation piece；12 gasket seals；13 microwell plates；14 microlayer models convey cavity；15 microlayer model transfer pipelines；16 microchannels；17
Bracket；18 porous culture plates cooperate frame；20 casing ontologies；21 high frequency oscillation microlayer models form plate；22 concussion piece mounting grooves；23 go out
Fluid apertures；24 connectors；25 delivery holes；26 liquid outlet microporess.
Specific embodiment
The utility model is described in further detail below with reference to embodiment, to enable those skilled in the art's reference say
Bright book text can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or combinations thereof.
As shown in figures 1-8, a kind of high-throughput hydrogel original position molding machine of the present embodiment, including casing ontology 20 and by
Crosslinking agent storage container 05, multi-channel high frequency concussion 07 and of microlayer model generator being successively set under upper in casing ontology 20
Multichannel microlayer model conveyor module 09；
Crosslinking agent storage container 05 shakes microlayer model generator 07 by the multi-channel high frequency of buckle 10 and its bottom and seals
Connection is closely connected by hasp and is realized between crosslinking agent storage container 05 and multi-channel high frequency concussion microlayer model generator 07
Sealing and anti-leakage, the through-hole that 05 bottom of crosslinking agent storage container opens up are connected to high frequency oscillation microlayer model generator upper end；
Multichannel microlayer model conveyor module 09 includes microlayer model conveying cavity 14, is set to microlayer model conveying 14 upper end of cavity
Microwell plate 13 and be set to multiple microlayer model transfer pipelines 15 of microlayer model conveying 14 lower end of cavity, 13 upper end of microwell plate and high
Frequency concussion microlayer model generator bottom connection, 15 lower end of microlayer model transfer pipeline is connected to 04 upper end of porous culture plate.
Wherein, referring to Fig. 7 and 8,20 lower end of casing ontology is provided with for fixed support multichannel microlayer model conveyor module
09 bracket 17, the outer rim of 20 lower end of casing ontology are additionally provided with porous culture plate cooperation frame 18.Bracket 17 is used to support and consolidates
Determine multichannel microlayer model conveyor module 09, porous culture plate cooperates frame 18 to be used to be cooperatively connected with the porous culture plate 04 of lower end,
So as to be easy to implement being fixedly connected and being connected between fixed multichannel microlayer model conveyor module 09 and porous culture plate 04.
High-throughput hydrogel original position molding machine by using high frequency oscillation or other modes generate crosslinking agent microlayer model into
The Quick cross-linking in situ of row hydrogel shakes microlayer model generator 07 by multi-channel high frequency and adopts in an advantageous embodiment
Crosslinking agent microlayer model is generated with the mode of high frequency oscillation.
Wherein, referring to Fig. 4, multi-channel high frequency shake microlayer model generator 07 include high frequency oscillation microlayer model formed plate 21,
It is provided with the multiple concussion piece mounting grooves 22 and be equipped with earthquake piece mounting groove 22 that high frequency oscillation microlayer model is formed on plate 21
Interior high frequency oscillation piece 11.The liquid out that lower end is connected to multichannel microlayer model conveyor module 09 is offered in concussion piece mounting groove 22
Hole 23.Crosslinking agent in storage container generates microlayer model by high frequency oscillation piece 11, and it is defeated to flow downwardly into multichannel microlayer model
Module 09 is sent, then is uniformly delivered to porous culture plate 04.
Wherein, referring to Fig. 5,13 upper end of microwell plate is provided with connector 24, and microwell plate 13 is internally provided with microchannel 16, even
The liquid that goes out that upper end and high frequency oscillation microlayer model are formed on plate 21 is offered in connector 24 to be connected to and the feed liquor of lower end and microchannel 16
The delivery hole 25 of hole connection.
Wherein, gasket seal 12 is additionally provided on delivery hole 25.Realize that connector 24 and high frequency shake by gasket seal 12
The tight fit connection that piece occurs for microlayer model is swung, the uniform conveying of microlayer model is realized by using microchannel 16, changes multichannel
The cellular structure of microlayer model conveyor module 09 can be adapted to different size porous culture plate 04.
Wherein, microchannel 16 includes multiple liquid outlet microporess 26, and multiple liquid outlet microporess 26 convey 14 lower end of cavity with microlayer model
Multiple microlayer model transfer pipelines 15 correspond connection, the liquid outlets of multiple microlayer model transfer pipelines 15 is porous with its lower end
Multiple micropores on culture plate 04 correspond connection.I.e. 26 quantity of liquid outlet micropores, 15 quantity of microlayer model transfer pipeline with it is porous
Micropore quantity on culture plate 04 corresponds to identical, and it is micro- that crosslinking agent microlayer model is averagely oriented to each of porous culture plate 04
Hole guarantees the consistency of each microporous completed state；In addition to be set by 26 quantity of liquid outlet micropores on microchannel 16
It counts, and design matched 15 quantity of microlayer model transfer pipeline to match each specification porous culture plate 04 common in the market.
Wherein, 20 upper end of casing ontology is provided with the cover board 01 slidably opened, and is additionally provided on 20 outer wall of casing ontology
Power knob 03, control circuit board 08 and battery charging interface 02 are additionally provided with rechargeable battery 06 on casing ontology 20.Each electricity
Road module can be easy to disassemble.The utility model both can directly be powered by external power supply, can also by rechargeable battery 06 into
Row power supply, is controlled by control circuit board 08.
In one embodiment, referring to Fig. 6, microchannel 16 has 16 liquid outlet microporess 26, and multichannel microlayer model conveys mould
Block 09 includes 6 groups, and porous culture plate 04 is corresponding 96 orifice plate.And with the high-throughput dimensional culture combination seaweed of stem cell
It is illustrated for the high-throughput formed in situ of sour sodium hydrogel, it is thin that sodium alginate and calcium chloride, which can carry out cross moulding,
The bracket 17 of born of the same parents' offer dimensional culture.Certain density sodium alginate is mixed according to a certain percentage with cell suspension, and is distributed
In 96 orifice plates, by the cover board 01 of sliding opening device, suitable concentration crosslinking agent calcium chloride solution is imported into crosslinking agent storage
In container 05 and cover board 01 is covered, device is integrally placed on 96 orifice plates for filling sodium alginate and cell suspension, electricity is clicked
03 starter of source button, is closed after a period of time and transfer device is the hydrogel original position molding for completing 96 channels, addition
Cell culture medium covers porous plate, and being sent into cell incubator can be realized the dimensional culture of cell.
In the description of the present invention, it should be understood that term " center ", " longitudinal direction ", " transverse direction ", " length ", " width
Degree ", " thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside", " suitable
The orientation or positional relationship of the instructions such as hour hands ", " counterclockwise ", " axial direction ", " radial direction ", " circumferential direction " is orientation based on the figure
Or positional relationship, be merely for convenience of describing the present invention and simplifying the description, rather than the device of indication or suggestion meaning or
Element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as the limit to the utility model
System.
It is not only in the description and the implementation although the embodiments of the present invention have been disclosed as above
Listed utilization, it can be applied to various fields suitable for the present invention completely, for those skilled in the art,
Other modifications may be easily implemented, therefore without departing from the general concept defined in the claims and the equivalent scope, this reality
It is not limited to specific details with novel.
Claims (9)
1. a kind of high throughput hydrogel original position molding machine, which is characterized in that including casing ontology and by setting gradually under upper
Crosslinking agent storage container, multi-channel high frequency concussion microlayer model generator and the conveying of multichannel microlayer model in the casing ontology
Module；
The crosslinking agent storage container shakes microlayer model generator by the multi-channel high frequency of buckle and its bottom and is tightly connected, institute
The through-hole that crosslinking agent storage container bottom opens up is stated to be connected to the high frequency oscillation microlayer model generator upper end；
The multichannel microlayer model conveyor module includes microlayer model conveying cavity, is set to microlayer model conveying cavity upper end
Microwell plate and the multiple microlayer model transfer pipelines for being set to microlayer model conveying cavity lower end, the microwell plate upper end with it is described
The connection of high frequency oscillation microlayer model generator bottom, microlayer model transfer pipeline lower end is connected to porous culture plate upper end.
2. high throughput hydrogel original position molding machine according to claim 1, which is characterized in that the casing ontology lower end
It is provided with for the fixed bracket for supporting the multichannel microlayer model conveyor module, the outer rim of the casing ontology lower end is also set up
There is porous culture plate to cooperate frame.
3. high throughput hydrogel original position molding machine according to claim 1, which is characterized in that the multi-channel high frequency shake
Swing microlayer model generator include high frequency oscillation microlayer model formed plate, be provided with the high frequency oscillation microlayer model formed it is multiple on plate
Concussion piece mounting groove and the high frequency oscillation piece being equipped in the concussion piece mounting groove.
4. high throughput hydrogel original position molding machine according to claim 3, which is characterized in that the concussion piece mounting groove
Inside offer the fluid hole that lower end is connected to the multichannel microlayer model conveyor module.
5. high throughput hydrogel original position molding machine according to claim 4, which is characterized in that the microwell plate upper end is set
It is equipped with connector, the microwell plate is internally provided with microchannel, and upper end is offered in the connector and the high frequency oscillation is micro-
The delivery hole for going out liquid connection and lower end is connected to the inlet opening of the microchannel on droplet formation plate.
6. high throughput hydrogel original position molding machine according to claim 5, which is characterized in that the microchannel includes more
Multiple microlayer model transfer pipelines one of a liquid outlet micropores, the multiple liquid outlet micropores and microlayer model conveying cavity lower end are a pair of
It should be connected to, multiple micropores on the liquid outlet of the multiple microlayer model transfer pipeline and the porous culture plate of its lower end correspond
Connection.
7. high throughput hydrogel original position molding machine according to claim 6, which is characterized in that also set on the delivery hole
It is equipped with gasket seal.
8. high throughput hydrogel original position molding machine according to claim 1, which is characterized in that the casing ontology upper end
It is provided with the cover board slidably opened, power knob, control circuit board and battery is additionally provided on the casing ontology outer wall and fills
Electrical interface.
9. high throughput hydrogel original position molding machine according to claim 1, which is characterized in that on the casing ontology also
It is provided with rechargeable battery.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821547880.4U CN209696867U (en) | 2018-09-21 | 2018-09-21 | High-throughput hydrogel original position molding machine |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201821547880.4U CN209696867U (en) | 2018-09-21 | 2018-09-21 | High-throughput hydrogel original position molding machine |
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| CN209696867U true CN209696867U (en) | 2019-11-29 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109173944A (en) * | 2018-09-21 | 2019-01-11 | 中国科学院苏州生物医学工程技术研究所 | High-throughput hydrogel original position molding machine |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109173944A (en) * | 2018-09-21 | 2019-01-11 | 中国科学院苏州生物医学工程技术研究所 | High-throughput hydrogel original position molding machine |
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