CN209508278U - The bionical culture apparatus of cell - Google Patents
The bionical culture apparatus of cell Download PDFInfo
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- CN209508278U CN209508278U CN201820662930.7U CN201820662930U CN209508278U CN 209508278 U CN209508278 U CN 209508278U CN 201820662930 U CN201820662930 U CN 201820662930U CN 209508278 U CN209508278 U CN 209508278U
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Abstract
The utility model provides a kind of bionical culture apparatus of cell, and the bionical culture apparatus of cell includes: injecting unit, and the both ends of the injecting unit are separately connected first pipe and second pipe；Culture chamber, the culture chamber have the first import and first outlet, and the first pipe connects the first outlet, and the second pipe connects first import；Isolation film, the isolation film is suitable for entrapped cell, but penetrates culture solution；The isolation film is arranged in the culture chamber, and the culture chamber is divided into first part, second part and Part III；The height of the roof of the second part is higher than the height of the roof of the first part and Part III；The second part has the second import and second outlet, and second import and second outlet are suitable for passing through for cell.The utility model can imitate heartbeat, realize three-dimensional bionic culturing, improve cell culture quality.
Description
Technical field
The utility model relates to cell culture, the in particular to bionical culture apparatus of cell.
Background technique
To realize cell three-dimensional large-scale culture, need to provide a suitable three-dimensional microenvironment for cell.It is main at present
Mode are as follows:
1. bioreactor, the main mixing for realizing three-dimensional carrier by way of stirring.Main deficiency is: biology is anti-
Answering device, there are shearing forces, influence cell growth.
2. perfusion culture, the injection for mainly passing through fresh medium guarantees the abundance of cytotrophy when large-scale culture,
And the removal of metabolite.Main deficiency is: although perfusion culture can guarantee the nutrition of culture solution, being unfavorable for culture solution
With mixing well for cell, bionical growing environment is provided for cell.
Utility model content
To solve the deficiency in above-mentioned prior art, the utility model provides a kind of bionical culture apparatus of cell,
The three-dimensional bionic culturing for imitating heartbeat is realized, cell culture quality is improved.
The purpose of this utility model is achieved through the following technical solutions:
A kind of bionical culture apparatus of cell, the bionical culture apparatus of cell include:
The both ends of injecting unit, the injecting unit are separately connected first pipe and second pipe；
Culture chamber, the culture chamber have the first import and first outlet, and the first pipe connects the first outlet,
The second pipe connects first import；
Isolation film, the isolation film is suitable for entrapped cell, but penetrates culture solution；The isolation film is arranged in the culture chamber
It is interior, the culture chamber is divided into first part, second part and Part III；The height of the roof of the second part is higher than
The height of the roof of the first part and Part III；The second part has the second import and a second outlet, and described the
Two imports and second outlet are suitable for passing through for cell.
According to the above-mentioned bionical culture apparatus of cell, optionally, the isolation film includes:
First isolation film and the second isolation film, first isolation film is identical with the tip height of first part, and described
Two isolation films are identical with the tip height of Part III.
According to the above-mentioned bionical culture apparatus of cell, optionally, the second part is also included
Third outlet, the third exit, which has, retains the cell but the film through culture solution.
According to the above-mentioned bionical culture apparatus of cell, it is preferable that the second outlet and third outlet setting are described the
The bottom of two parts.
According to the above-mentioned bionical culture apparatus of cell, optionally, the bionical culture apparatus of cell further comprises:
Temperature conditioning unit, the temperature conditioning unit include heater and temperature sensor, and the heater and temperature sensor are set
It sets in the culture chamber.
According to the above-mentioned bionical culture apparatus of cell, it is preferable that the heater uses ITO heating film.
According to the above-mentioned bionical culture apparatus of cell, it is preferable that the top of the second part is arranged in second import.
Compared with prior art, the utility model has the beneficial effect that
1. providing a kind of three-dimensional bionic culturing method of imitative heartbeat, the quality of cell culture is improved；
2. providing a kind of suitable culture solution displacement and cell blending manner, while guaranteeing culture solution nutrition, reduce
Shearing force when cell mixes.
Detailed description of the invention
Referring to attached drawing, the disclosure of the utility model will be easier to understand.Skilled addressee readily understands that
Be: these attached drawings are used only for illustrating the technical solution of the utility model, and are not intended to the protection to the utility model
Range is construed as limiting.In figure:
Fig. 1 is the structure diagram according to the bionical culture apparatus of cell of the utility model embodiment 1.
Specific embodiment
Fig. 1 and following description describe the optional embodiments of the utility model to instruct those skilled in the art how real
Apply and reproduce the utility model.In order to instruct technical solutions of the utility model, it has simplified or omitted some conventional aspects.Ability
Field technique personnel should be understood that the variation or replacement from these embodiments will be in the scope of the utility model.This field skill
Art personnel should be understood that following characteristics can be combined in various ways to form multiple modifications of the utility model.This reality as a result,
Following optional embodiments are not limited to novel, and are only limited by the claims and their equivalents.
Embodiment 1:
Fig. 1 schematically illustrates the structure diagram of the bionical culture apparatus of cell of the utility model embodiment 1, such as Fig. 1
Shown, the bionical culture apparatus of cell includes:
Injecting unit 8, the both ends of the injecting unit are separately connected first pipe 4 and second pipe；Injecting unit provides
The injection of imitative heartbeat, the frequency and injection volume of beating are adjustable；Injecting unit provides culture solution in first pipe, second pipe
With the driving force to circulate in culture chamber, after cell and culture solution are put into culture chamber, culture solution needs to fill entire first pipe
Road, the second pipeline and injecting unit, after instrument starting, injecting unit starts to inject according to the frequency of injection of 0.5-1Hz, Zhi Daopei
Supporting terminates, and injecting unit is closed.
Culture chamber 1, the culture chamber have the first import and first outlet, and the first pipe connection described first goes out
Mouthful, the second pipe connects first import；
Isolation film 6, the isolation film is suitable for entrapped cell or cell carrier and cell, but penetrates culture solution；The isolation
Film is arranged in the culture chamber, and the culture chamber is divided into first part 15, second part and Part III 7；Described
The height of the roof of two parts is higher than the height of the roof of the first part and Part III；The second part has second
Import 12 and second outlet 14, second import and second outlet are suitable for passing through for cell, and the second import can also be used in carrier
10 pass through, and second outlet is for being discharged cell；
Third outlet 13, the third exit, which has, retains the cell but the film through culture solution, useless for being discharged
Culture solution, but entrapped cell or carrier and cell；
Temperature conditioning unit, the temperature conditioning unit adjust the temperature in the culture chamber, so that meeting the temperature of cell culture；
Gas control unit 11, the gas control unit adjust the gas content in the culture chamber, so that meeting thin
The requirement of born of the same parents' culture.
According to the cell bionic culture method of the above-mentioned bionical culture apparatus of cell, the cell bionic culture method includes
Following steps:
(A1) fresh culture solution is injected in second part by the second import, and the position of liquid level 5 is lower than in second part
The roof of the second part；
(A2) under the action of injecting unit, culture solution is intermittently injected in the culture chamber, culture solution pass through every
Enter in second part from film, shake the culture solution in second part regularly, cell is suspended in the second part
In culture solution；
(A3) cell for terminating culture is discharged from the second outlet.
Embodiment 2:
According to the bionical culture apparatus of the cell of the utility model embodiment 1 and the application examples of method.
In the application example, as shown in Figure 1, isolation film includes the first isolation film and the second isolation film, first isolation
Film is identical with the tip height of first part, and second isolation film is identical with the tip height of Part III；Described second into
The top of the second part is arranged in mouth, and the bottom of the second part is arranged in second outlet and third outlet；Temperature conditioning unit
Including heater 2-3 and temperature sensor 9, the heater and temperature sensor are arranged in the culture chamber；The heating
Device uses ITO heating film.
According to the cell bionic culture method of the above-mentioned bionical culture apparatus of cell, the cell bionic culture method includes
Following steps:
(A1) culture solution is injected in second part by the second import, and the position of liquid level is lower than described second in second part
Partial roof；
(A2) under the action of injecting unit, culture solution is intermittently injected in the culture chamber, culture solution pass through every
Enter in second part from film, shake the culture solution in second part regularly, cell is suspended in the second part
In culture solution；The injecting unit, second pipe, is full of culture solution in first part and Part III at first pipe, and second
The height of liquid level is no more than the position on the top of isolation film in point, the liquid level and first part and Part III in second part
Roof maintains an equal level,
(A3) cell cultivated is discharged from the second outlet, and the culture solution that gives up is exported from third to be discharged.
Claims (7)
1. a kind of bionical culture apparatus of cell, it is characterised in that: the bionical culture apparatus of cell includes:
The both ends of injecting unit, the injecting unit are separately connected first pipe and second pipe；
Culture chamber, the culture chamber have the first import and first outlet, and the first pipe connects the first outlet, described
Second pipe connects first import；
Isolation film, the isolation film is suitable for entrapped cell, but penetrates culture solution；The isolation film is arranged in the culture chamber,
The culture chamber is divided into first part, second part and Part III；The height of the roof of the second part is higher than institute
State the height of the roof of first part and Part III；The second part have the second import and second outlet, described second
Import and second outlet are suitable for passing through for cell.
2. the bionical culture apparatus of cell according to claim 1, it is characterised in that: the isolation film includes:
First isolation film and the second isolation film, first isolation film is identical with the tip height of first part, described second every
It is identical with the tip height of Part III from film.
3. the bionical culture apparatus of cell according to claim 1, it is characterised in that: the second part also includes
Third outlet, the third exit, which has, retains the cell but the film through culture solution.
4. the bionical culture apparatus of cell according to claim 3, it is characterised in that: the second outlet and third outlet are set
It sets in the bottom of the second part.
5. the bionical culture apparatus of cell according to claim 1, it is characterised in that: the bionical culture apparatus of cell is into one
Step includes:
Temperature conditioning unit, the temperature conditioning unit include heater and temperature sensor, and the heater and temperature sensor setting exist
In the culture chamber.
6. the bionical culture apparatus of cell according to claim 5, it is characterised in that: the heater uses ITO heating film.
7. the bionical culture apparatus of cell according to claim 1, it is characterised in that: the second import setting described second
Partial top.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820662930.7U CN209508278U (en) | 2018-05-07 | 2018-05-07 | The bionical culture apparatus of cell |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820662930.7U CN209508278U (en) | 2018-05-07 | 2018-05-07 | The bionical culture apparatus of cell |
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| CN209508278U true CN209508278U (en) | 2019-10-18 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641952A (en) * | 2018-05-07 | 2018-10-12 | 宁波华仪宁创智能科技有限公司 | The bionical culture apparatus of cell and method |
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2018
- 2018-05-07 CN CN201820662930.7U patent/CN209508278U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641952A (en) * | 2018-05-07 | 2018-10-12 | 宁波华仪宁创智能科技有限公司 | The bionical culture apparatus of cell and method |
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Effective date of registration: 20201222 Address after: Area a, 4th floor, no.483, Xingnan Avenue, Nancun Town, Panyu District, Guangzhou City, Guangdong Province Patentee after: Guangzhou aibeitai Biotechnology Co.,Ltd. Patentee after: CHINA INNOVATION INSTRUMENT Co.,Ltd. Address before: Room 304, D Building, Kexin Building, 655 Xueshi Road, Yinzhou District, Ningbo City, Zhejiang Province, 315000 Patentee before: CHINA INNOVATION INSTRUMENT Co.,Ltd. |
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| TR01 | Transfer of patent right |
Effective date of registration: 20220325 Address after: Area a, 4th floor, no.483, Xingnan Avenue, Nancun Town, Panyu District, Guangzhou City, Guangdong Province Patentee after: Guangzhou aibeitai Biotechnology Co.,Ltd. Address before: Area a, 4th floor, no.483, Xingnan Avenue, Nancun Town, Panyu District, Guangzhou City, Guangdong Province Patentee before: Guangzhou aibeitai Biotechnology Co.,Ltd. Patentee before: Ningbo Huayi ningchuang Intelligent Technology Co., Ltd |
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| TR01 | Transfer of patent right |