CN111304086A - Cell culture negative pressure liquid change operating system - Google Patents
Cell culture negative pressure liquid change operating system Download PDFInfo
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- CN111304086A CN111304086A CN201911234458.2A CN201911234458A CN111304086A CN 111304086 A CN111304086 A CN 111304086A CN 201911234458 A CN201911234458 A CN 201911234458A CN 111304086 A CN111304086 A CN 111304086A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 64
- 239000007788 liquid Substances 0.000 title claims abstract description 33
- 230000008859 change Effects 0.000 title claims abstract description 15
- 235000015097 nutrients Nutrition 0.000 claims abstract description 39
- 238000007789 sealing Methods 0.000 claims abstract description 34
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 21
- 239000002699 waste material Substances 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000005086 pumping Methods 0.000 claims description 4
- 229910001069 Ti alloy Inorganic materials 0.000 claims description 3
- 238000000605 extraction Methods 0.000 abstract description 2
- 230000000630 rising effect Effects 0.000 abstract description 2
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- 230000005486 microgravity Effects 0.000 description 5
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- 238000005516 engineering process Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000012606 in vitro cell culture Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
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- 238000006467 substitution reaction Methods 0.000 description 1
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The invention discloses a cell culture negative pressure liquid change operation system which comprises a fixed bottom plate, wherein the top of the fixed bottom plate is fixedly connected with a nutrient solution storage cavity, a cell culture cavity, a peristaltic pump and a waste liquid collecting box respectively from left to right, and the top of the fixed bottom plate, which is positioned on the back of the cell culture cavity, is fixedly connected with a controller. This cell culture negative pressure trades liquid operating system, when adding the cell, take off it through the sealed lid of rotating, add nutrient solution and cell to the cell culture intracavity, then screw sealed lid, make the tight ring card of rising in the seal ring advance the tight inslot that rises, and sealed lid extrudees the tight gasbag that rises downwards and makes it expand to both sides, the tight gasbag that rises inflation is sealed up the gap between with sealed lid and the charge door, the operation of sealing up that carries on that can be quick, prevent air admission, and can be quick through the cell filter sieve with cell and nutrient solution separation, the extraction of the cell of being convenient for, it is more convenient to use.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a cell culture negative-pressure liquid changing operation system.
Background
The in vitro cell culture technology has become the basis of the biological research related to the cells, along with the development of space life science research, people combine the cell and tissue culture technology with the microgravity environment to generate a new field of tissue engineering research, namely microgravity tissue engineering, the research of cell culture and tissue construction under the condition of simulating space microgravity shows that the microgravity environment is favorable for the three-dimensional growth of the cells to form a tissue-like structure with functions, the cultured three-dimensional tissue is more close to normal body tissue from both the morphology and the gene expression, the effect of the microgravity on the cells can possibly provide a new way for the future tissue engineering and regenerative medicine research, the in vitro cell culture is a modern biological test technology which provides proper environment and nutrition supply for the cell growth, and the cells are very sensitive, the growth environment is very demanding, and the environment required by the cells must generally be kept constant, including: the laboratory routine operation of cell culture at present comprises that a tester regularly replaces a culture dish with fresh nutrient solution for cells in a sterile operating platform, and then the culture dish is placed in a constant-temperature incubator for culture.
When current cell culture equipment carries out cell culture, need change the nutrient solution of inside, but current cell culture equipment all needs artifical manual taking out the cell earlier when changing the nutrient solution, then pours into new nutrient solution into after the waste liquid is emptyd, uses very trouble to when sealing up the cell, the operation is complicated, need seal through a large amount of complicated structures.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a cell culture negative pressure liquid changing operation system, which solves the problems that when cells are sealed, the operation is complex, the cells can be sealed through a large number of complex structures, and the cells are taken out manually when the nutrient solution is changed by the conventional cell culture equipment, and then the waste liquid is poured and injected into new nutrient solution, so that the use is very troublesome.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a cell culture negative pressure liquid change operation system comprises a fixed base plate, wherein the top of the fixed base plate is fixedly connected with a nutrient solution storage cavity, a cell culture cavity, a peristaltic pump and a waste liquid collecting box respectively from left to right, the top of the fixed base plate is fixedly connected with a controller on the back of the cell culture cavity, the right side of the nutrient solution storage cavity is communicated with a first rubber pipeline, the right end of the first rubber pipeline is communicated with the left side of the cell culture cavity, the right side of the cell culture cavity is communicated with a second rubber pipeline, the right end of the second rubber pipeline is communicated with a water pumping port of the peristaltic pump, a water outlet of the peristaltic pump is communicated with a third rubber pipeline, the right end of the third rubber pipeline is communicated with the left side of the waste liquid collecting box, the surface of the first rubber pipeline is fixedly connected with an electromagnetic valve, and the surface of the second rubber pipeline is fixedly connected with, the cell culture device is characterized in that a cell filter screen is fixedly connected to the inner cavity of the filter cylinder, the top of the cell culture cavity is communicated with a feed inlet, the surface of the feed inlet is in threaded connection with a sealing cover, a sealing washer is fixedly connected to the bottom of the sealing cover, and a tensioning ring is fixedly connected to the bottom of the sealing washer.
Preferably, the cell filter screen is a titanium alloy filter screen, and the pore size of the filter screen is 4.3 μm.
Preferably, the surface of the nutrient solution storage cavity is fixedly connected with a first temperature controller and a first heater from top to bottom respectively, and the surface of the cell culture cavity is fixedly connected with a second temperature controller and a second heater respectively.
Preferably, the bottom of the tensioning ring penetrates through the top of the feeding port and extends to the inner cavity of the feeding port, and the inner cavity of the feeding port is provided with a tensioning groove matched with the surface of the tensioning ring.
Preferably, the surface of the feeding opening is sleeved with a tensioning airbag, and the surface of the tensioning airbag is abutted to the bottom of the sealing cover.
Preferably, the inner cavity of the cell culture chamber is slidably connected with a fixing frame, the inner cavity of the fixing frame is fixedly connected with a cell filter screen, the top of the fixing frame is fixedly connected with an ejector rod, and the surface of the ejector rod is fixedly connected with a handle.
Preferably, the surface of the controller is fixedly connected with a touch display screen, the controller comprises a central control unit, a temperature adjusting unit and a timing unit, and the output end of the central control unit is respectively connected with the input ends of the electromagnetic valve, the first temperature controller, the second temperature controller and the timing unit.
Preferably, the central control unit is connected with the touch display screen in a bidirectional mode, the output end of the temperature adjusting unit is connected with the input end of the central control unit, the output end of the first temperature controller is connected with the input end of the first heater, the output end of the second temperature controller is connected with the input end of the second heater, and the output end of the timing unit is connected with the input end of the peristaltic pump.
(III) advantageous effects
The invention provides a cell culture negative pressure liquid changing operation system. Compared with the prior art, the method has the following beneficial effects:
(1) the cell culture negative pressure liquid changing operation system is characterized in that an electromagnetic valve is fixedly connected to the surface of a first rubber pipeline, a filter cylinder is fixedly connected to the surface of a second rubber pipeline, a cell filter screen is fixedly connected to the inner cavity of the filter cylinder, the top of a cell culture cavity is communicated with a feed inlet, a sealing cover is in threaded connection with the surface of the feed inlet, a sealing washer is fixedly connected to the bottom of the sealing cover, a tensioning ring is fixedly connected to the bottom of the sealing washer, the bottom of the tensioning ring penetrates through the top of the feed inlet and extends to the inner cavity of the feed inlet, a tensioning groove matched with the surface of the tensioning ring is formed in the inner cavity of the feed inlet, a tensioning air bag is sleeved on the surface of the feed inlet, the surface of the tensioning air bag is abutted against the bottom of the sealing cover, a fixing frame is slidably connected to the, the top fixedly connected with ejector pin of mount, the fixed surface of ejector pin is connected with the handle, when adding the cell, take off it from the charge door through the sealed lid of rotating, add nutrient solution and cell in to the cell culture intracavity, then screw sealed lid, make the tight circle card of rising in the seal ring advance the tight inslot that rises, and sealed lid extrudees the tight gasbag that rises downwards and makes it expand to both sides, the tight gasbag that rises expands seals the gap between sealed lid and the charge door, can be quick seal operation, prevent air admission, and can be quick separate cell and nutrient solution through the cell filter sieve, the extraction of the cell of being convenient for, it is more convenient to use.
(2) The cell culture negative pressure liquid change operation system comprises a fixed base plate, a nutrient solution storage cavity, a cell culture cavity, a peristaltic pump and a waste liquid collecting box which are fixedly connected with the top of the fixed base plate from left to right, a controller is fixedly connected with the top of the fixed base plate and the back of the cell culture cavity, a first rubber pipeline is communicated with the right side of the nutrient solution storage cavity, the right end of the first rubber pipeline is communicated with the left side of the cell culture cavity, a second rubber pipeline is communicated with the right side of the cell culture cavity, the right end of the second rubber pipeline is communicated with a water pumping port of the peristaltic pump, a water outlet of the peristaltic pump is communicated with a third rubber pipeline, the right end of the third rubber pipeline is communicated with the left side of the waste liquid collecting box, when the nutrient solution is changed, only an electromagnetic valve needs to be opened, the waste liquid can be pumped out through the peristaltic pump and filtered by a filter cylinder, and under the condition of, the nutrient solution can be quickly pumped in while the waste liquid is discharged.
(3) The cell culture negative pressure liquid change operation system is characterized in that a touch display screen is fixedly connected to the surface of a controller, the controller comprises a central control unit, a temperature adjusting unit and a timing unit, the output end of the central control unit is respectively connected with the input ends of an electromagnetic valve, a first temperature controller, a second temperature controller and the timing unit, the central control unit is in bidirectional connection with the touch display screen, the output end of the temperature adjusting unit is connected with the input end of the central control unit, the output end of the first temperature controller is connected with the input end of a first heater, the output end of the second temperature controller is connected with the input end of a second heater, the output end of the timing unit is connected with the input end of a peristaltic pump, the first temperature controller and the first heater are respectively and fixedly connected to the surface of a nutrient solution storage cavity from top to bottom, the second temperature controller and the second heater are respectively and fixedly connected to the surface of a cell culture, control through central control unit, can be so that nutrient solution storage chamber and cell culture chamber can keep a invariable temperature always for can cultivate the cell under the environment that suits more, it is better to cultivate the effect, and control is convenient.
Drawings
FIG. 1 is a perspective view of the structure of the present invention;
FIG. 2 is a schematic block diagram of the system of the present invention;
FIG. 3 is a sectional view of the structure of the cell culture chamber according to the present invention;
FIG. 4 is an enlarged view of a portion of the structure at A in the present invention 1;
fig. 5 is a cross-sectional view of a filter cartridge construction of the present invention.
In the figure, 1-a fixed bottom plate, 2-a nutrient solution storage cavity, 3-a cell culture cavity, 4-a peristaltic pump, 5-a waste liquid collecting box, 6-a controller, 7-a touch display screen, 8-a first rubber pipeline, 9-a second rubber pipeline, 10-a third rubber pipeline, 11-an electromagnetic valve, 12-a filter cylinder, 13-a cell filter screen, 14-a first temperature controller, 15-a first heater, 16-a second temperature controller, 17-a second heater, 18-a feed inlet, 19-a sealing cover, 20-a sealing gasket, 21-a tensioning ring, 22-a tensioning groove, 23-a tensioning airbag, 24-a fixed frame, 25-a cell filter screen, 26-a mandril, 61-a central control unit, 62-a temperature adjusting unit, 63-timing unit.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, an embodiment of the present invention provides a technical solution: a cell culture negative pressure liquid change operation system comprises a fixed base plate 1, the top of the fixed base plate 1 is fixedly connected with a nutrient solution storage cavity 2, a cell culture cavity 3, a peristaltic pump 4 and a waste liquid collecting box 5 from left to right respectively, the inner cavity of the cell culture cavity 3 is connected with a fixed frame 24 in a sliding manner, the inner cavity of the fixed frame 24 is fixedly connected with a cell filter screen 25, the top of the fixed frame 24 is fixedly connected with an ejector rod 26, the surface of the ejector rod 26 is fixedly connected with a handle, the surface of the nutrient solution storage cavity 2 is fixedly connected with a first temperature controller 14 and a first heater 15 from top to bottom respectively, the first temperature controller 14, the first heater 15, a second temperature controller 18 and a second heater 17 are all communicated with a power supply, the surface of the cell culture cavity 3 is fixedly connected with a second temperature controller 16 and a second heater 17 respectively, the top of the fixed base plate 1 is fixedly connected with a controller 6 on the back of the, the surface of the controller 6 is fixedly connected with a touch display screen 7, the controller 6 comprises a central control unit 61, a temperature adjusting unit 62 and a timing unit 63, the central control unit 61 is in bidirectional connection with the touch display screen 7, the output end of the temperature adjusting unit 62 is connected with the input end of the central control unit 61, the central control unit 61 is used for controlling, the nutrient solution storage cavity 2 and the cell culture cavity 3 can always keep a constant temperature, cells can be cultured in a more suitable environment, the culture effect is better, the control is convenient, the output end of a first temperature controller 14 is connected with the input end of a first heater 15, the output end of a second temperature controller 16 is connected with the input end of a second heater 17, the output end of the timing unit 63 is connected with the input end of a peristaltic pump 4, and the output end of the central control unit 61 is respectively connected with an electromagnetic valve 11, The input ends of the first temperature controller 14, the second temperature controller 16 and the timing unit 63 are connected, when the nutrient solution is replaced, only the electromagnetic valve 11 needs to be opened, the waste solution can be pumped out through the peristaltic pump 4 and filtered by the filter cartridge 12, and under the condition of negative pressure, the nutrient solution is quickly sucked from the nutrient solution storage chamber 2, the nutrient solution can be quickly pumped in while the waste solution is discharged, the right side of the nutrient solution storage chamber 2 is communicated with the first rubber pipeline 8, the right end of the first rubber pipeline 8 is communicated with the left side of the cell culture chamber 3, the right side of the cell culture chamber 3 is communicated with the second rubber pipeline 9, the right end of the second rubber pipeline 9 is communicated with a water pumping port of the peristaltic pump 4, the water outlet of the peristaltic pump 4 is communicated with the third rubber pipeline 10, the right end of the third rubber pipeline 10 is communicated with the left side of the waste solution collection tank 5, the surface of the first rubber pipeline 8 is fixedly connected with the, the surface of the second rubber pipeline 9 is fixedly connected with a filter cylinder 12, the inner cavity of the filter cylinder 12 is fixedly connected with a cell filter screen 13, the cell filter screen 13 is a titanium alloy filter screen, the aperture of the filter screen is 4.3 mu m, the top of the cell culture cavity 3 is communicated with a feed inlet 18, the surface of the feed inlet 18 is sleeved with a tensioning airbag 23, the surface of the tensioning airbag 23 is mutually abutted with the bottom of a sealing cover 19, the surface of the feed inlet 18 is in threaded connection with a sealing cover 19, the bottom of the sealing cover 19 is fixedly connected with a sealing washer 20, the bottom of the sealing washer 20 is fixedly connected with a tensioning ring 21, the bottom of the tensioning ring 21 penetrates through the top of the feed inlet 18 and extends to the inner cavity of the feed inlet 18, the inner cavity of the feed inlet 18 is provided with a tensioning groove 22 matched with the surface of the tensioning ring 21, when cells are added, the sealing cover 19 is taken down from the feed inlet 18 by rotating, then the sealing cover 19 is screwed, so that the tensioning ring 21 in the sealing washer 20 is clamped into the tensioning groove 22, the sealing cover 19 downwards extrudes the tensioning airbag 23 to enable the tensioning airbag 23 to expand towards two sides, the tensioning airbag 23 expands to seal a gap between the sealing cover 19 and the feeding port 18, sealing operation can be rapidly performed, air is prevented from entering, cells and nutrient solution can be rapidly separated through the cell filter sieve 25, the cells can be conveniently extracted, and the use is more convenient.
When the device is used, nutrient solution is added into the nutrient solution storage cavity 2 along a pipeline, the sealing cover 19 is rotated to be taken down from the feeding port 18, the nutrient solution and cells are added into the cell culture cavity 3, then the sealing cover 19 is screwed, so that the tensioning ring 21 in the sealing washer 20 is clamped into the tensioning groove 22, the sealing cover 19 downwards extrudes the tensioning airbag 23 to expand towards two sides, the temperature is set through the temperature adjusting unit 62 on the controller 6 at the moment, the first heater 15 is controlled to heat through the first temperature controller 14, the second heater 17 is controlled to heat through the second temperature controller 16, when the nutrient solution needs to be replaced, the electromagnetic valve 11 is opened, the peristaltic pump 4 is started to pump the waste solution out along the second rubber pipeline 10, when the waste solution passes through the filter cylinder 12, the cells are filtered out through the cell filter screen 13, the waste solution is pumped out and discharged into the waste solution collecting box 5, at the moment, the nutrient solution in the nutrient solution storage cavity 2 under negative pressure is sucked into the cell culture cavity 3 along the first rubber pipeline 8 to be, after the culture is finished, the mandril 26 is pulled to drive the fixing frame 24 to move upwards, cells are screened out through the cell filter screen 25, and then the cells are sucked through the dropper.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides a cell culture negative pressure trades liquid operating system, includes PMKD (1), the top of PMKD (1) from left to right respectively fixed connection have nutrient solution storage chamber (2), cell culture chamber (3), peristaltic pump (4) and waste liquid collecting box (5), the back fixedly connected with controller (6) that the top of PMKD (1) just is located cell culture chamber (3), its characterized in that: the right side of the nutrient solution storage cavity (2) is communicated with a first rubber pipeline (8), the right end of the first rubber pipeline (8) is communicated with the left side of the cell culture cavity (3), the right side of the cell culture cavity (3) is communicated with a second rubber pipeline (9), the right end of the second rubber pipeline (9) is communicated with a water pumping port of the peristaltic pump (4), a water outlet of the peristaltic pump (4) is communicated with a third rubber pipeline (10), the right end of the third rubber pipeline (10) is communicated with the left side of the waste liquid collecting box (5), the surface of the first rubber pipeline (8) is fixedly connected with an electromagnetic valve (11), the surface of the second rubber pipeline (9) is fixedly connected with a filter cylinder (12), the inner cavity of the filter cylinder (12) is fixedly connected with a cell filter screen (13), and the top of the cell culture cavity (3) is communicated with a feeding port (18), the surface of the feeding port (18) is in threaded connection with a sealing cover (19), the bottom of the sealing cover (19) is fixedly connected with a sealing washer (20), and the bottom of the sealing washer (20) is fixedly connected with a tensioning ring (21).
2. The cell culture negative pressure liquid change operation system according to claim 1, wherein: the cell filter screen (13) is a titanium alloy filter screen, and the aperture of the filter screen is 4.3 mu m.
3. The cell culture negative pressure liquid change operation system according to claim 1, wherein: the surface of the nutrient solution storage cavity (2) is fixedly connected with a first temperature control instrument (14) and a first heater (15) from top to bottom respectively, and the surface of the cell culture cavity (3) is fixedly connected with a second temperature control instrument (16) and a second heater (17) respectively.
4. The cell culture negative pressure liquid change operation system according to claim 1, wherein: the bottom of the tensioning ring (21) penetrates through the top of the feeding port (18) and extends to the inner cavity of the feeding port (18), and a tensioning groove (22) matched with the surface of the tensioning ring (21) is formed in the inner cavity of the feeding port (18).
5. The cell culture negative pressure liquid change operation system according to claim 1, wherein: the surface of the charging opening (18) is sleeved with a tension airbag (23), and the surface of the tension airbag (23) is abutted against the bottom of the sealing cover (19).
6. The cell culture negative pressure liquid change operation system according to claim 1, wherein: the inner cavity of the cell culture cavity (3) is connected with a fixing frame (24) in a sliding mode, the inner cavity of the fixing frame (24) is fixedly connected with a cell filter screen (25), the top of the fixing frame (24) is fixedly connected with an ejector rod (26), and the surface of the ejector rod (26) is fixedly connected with a handle.
7. The cell culture negative pressure liquid change operation system according to claim 3, wherein: the surface of the controller (6) is fixedly connected with a touch display screen (7), the controller (6) comprises a central control unit (61), a temperature adjusting unit (62) and a timing unit (63), and the output end of the central control unit (61) is connected with the input ends of the electromagnetic valve (11), the first temperature controller (14), the second temperature controller (16) and the timing unit (63) respectively.
8. The cell culture negative pressure liquid change operation system according to claim 7, wherein: central control unit (61) and touch display screen (7) realize the both way junction, the output and the input of central control unit (61) of temperature regulation unit (62) are connected, the output and the input of first heater (15) of first temperature control appearance (14) are connected, the output and the input of second heater (17) of second temperature control appearance (16) are connected, the output and the input of peristaltic pump (4) of timing unit (63) are connected.
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| CN201911234458.2A CN111304086A (en) | 2019-12-05 | 2019-12-05 | Cell culture negative pressure liquid change operating system |
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| CN113337399A (en) * | 2021-04-26 | 2021-09-03 | 张庆兰 | Plant cell culture device of daylighting floats |
| CN113331065A (en) * | 2021-06-07 | 2021-09-03 | 温州医科大学附属第二医院（温州医科大学附属育英儿童医院） | Animal experiment sealing isolator |
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| CN207906433U (en) * | 2018-01-07 | 2018-09-25 | 新昌县焕宏农业有限公司 | A kind of sealing ring sealing structure between Side Template |
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