CN204058483U - For animal cell culture can the culture tank of quick change liquid soon - Google Patents
For animal cell culture can the culture tank of quick change liquid soon Download PDFInfo
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- CN204058483U CN204058483U CN201420527637.1U CN201420527637U CN204058483U CN 204058483 U CN204058483 U CN 204058483U CN 201420527637 U CN201420527637 U CN 201420527637U CN 204058483 U CN204058483 U CN 204058483U
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Abstract
The utility model relate to a kind of for animal cell culture can the culture tank of quick change liquid soon, comprise inner hollow, the tank body of top end opening, be provided with a cover plate tank body opening part is dismountable, a screen plate that can move up and down along inner tank wall is provided with in tank body, movablely on described cover plate be inserted with the pumping pipe and vapor pipe that bottom is connected with screen plate respectively, the bottom import of described pumping pipe is positioned at the top of screen plate, valve is provided with in the exit, top of pumping pipe, the bottom import of described vapor pipe is positioned at the below of screen plate, valve is provided with in the exit, top of vapor pipe, described cover plate is provided with and the sterile air inlet pipe be connected in tank body and liquid-inlet pipe, described sterile air inlet pipe and liquid-inlet pipe are equipped with valve, sealing member is equipped with at pumping pipe with between vapor pipe and cover plate.This culture tank has higher changes liquid efficiency, and simple to operate, has good practicality.
Description
Technical field
The utility model relates to a kind of animal cell culture tank, particularly relate to a kind of for animal cell culture can the culture tank of quick change liquid soon.
Background technology
In vaccine product triturating, for reducing trouble necessary in the middle part of purification work, reduce the residual quantity of bovine serum albumin in the finished product, generally terminate in animal cell culture, before connecing virus, the substratum of serum need be changed into the substratum of serum-free, the liquid changing device of existing animal cell culture generally adopts the precipitator method, namely treat that the cell precipitation in nutrient solution goes down, released by the substratum containing serum above cell, and then add the substratum of serum-free, it is oversize that this method changes the liquid time, and it is not thorough to change liquid, has larger deficiency.For overcoming the drawback of aforesaid method, Chinese utility model patent CN203307345U discloses a kind of liquid changing device of animal cell culture, comprise culture tank, at the top of culture tank or side, nutrient solution liquid-inlet pipe is set, in the bottom of culture tank, bottom valve is set, be provided with stirring rake in the inside of culture tank, the bottom of described culture tank is also provided with changes liquid valve.The described liquid valve that changes comprises valve seat, valve gap, filter core, valve body, valve rod, knob, adapter sleeve and handwheel.This liquid changing device utilizes the liquid valve that changes be arranged at bottom culture tank to carry out post-filter drainage, can save time compared to the precipitator method, and it is also comparatively thorough to change liquid, but this device is because changing liquid valve filtration sections limitation of size, in use to change liquid efficiency still not high enough, and its structure is also comparatively complicated, manufacturing cost is higher.
Utility model content
For solving the deficiencies in the prior art, the utility model provide a kind of for animal cell culture can the culture tank of quick change liquid soon, it has and higher changes liquid efficiency, and simple to operate, has good practicality.
For achieving the above object, of the present utility modelly the culture tank of quick change liquid soon can comprise inner hollow for animal cell culture, the tank body of top end opening, be provided with a cover plate tank body opening part is dismountable, a screen plate that can move up and down along inner tank wall is provided with in tank body, movablely on described cover plate be inserted with the pumping pipe and vapor pipe that bottom is connected with screen plate respectively, the bottom import of described pumping pipe is positioned at the top of screen plate, valve is provided with in the exit, top of pumping pipe, the bottom import of described vapor pipe is positioned at the below of screen plate, valve is provided with in the exit, top of vapor pipe, described cover plate is provided with and the sterile air inlet pipe be connected in tank body and liquid-inlet pipe, described sterile air inlet pipe and liquid-inlet pipe are equipped with valve, sealing member is equipped with at pumping pipe with between vapor pipe and cover plate.
Adopt above-mentioned technical scheme, when needs carry out changing liquid, can export at pumping pipe and connect liquid absorbing pump, and make sterile air inlet pipe connect upper sterile air pipeline, then the valve in pumping pipe and sterile air inlet pipe is opened respectively, promotion pumping pipe and vapor pipe make screen plate move down along inner tank wall, thus the substratum in tank body in nutrient solution can be made to arrive through filter plates the top of screen plate, and finally extract out through pumping pipe, zooblast in nutrient solution is then retained in tank body because screen plate stops, owing to considerably increasing filtration area, thus the raising greatly of changing liquid efficiency can be realized.After in tank body, substratum exhausts, new substratum can be added in tank body through liquid-inlet pipe, and in drawing liquid process or after drawing liquid, if have zooblast or other solid blockade screen plate mesh, then can close pumping pipe valve, and make screen plate to move on nutrient solution liquid level, sterile air is entered in tank body, then screen plate mesh can be washed open by the valve opened on vapor pipe via sterile air.
As the restriction to aforesaid way, described cover plate is the shell structure of inner hollow, and described sterile air inlet pipe is communicated in the inside of cover plate, is provided with the production well be connected in tank body in the bottom of cover plate, and described sealing member is located at the top of cover plate.Cover plate is made to be hollow structure, the positive seal that can realize cover plate and pumping pipe and vapor pipe junction via the sterile air that pours in cover plate like this, and cover plate be hollow structure also can via its top and the bottom to the support of pumping pipe and vapor pipe but pumping pipe and vapor pipe arrange more stable.
As the restriction to aforesaid way, be provided with the projection movement of pumping pipe and vapor pipe being carried out to guide support in the bottom of described cover plate.Arrange when projection can make pumping pipe and vapor pipe move up and down more stable, thus can ensure all to maintain rational gap between all edges of screen plate and inner tank wall.
As the restriction to aforesaid way, be connected with drawing handle on the top of pumping pipe and vapor pipe.Arrange drawing handle can make via pumping pipe and vapor pipe convenient to moving up and down of screen plate.
In sum, adopt the technical solution of the utility model, that can improve nutrient solution changes liquid efficiency, and simple to operate, has good practicality.
Accompanying drawing explanation
Do further to describe in detail to the utility model below in conjunction with the drawings and the specific embodiments:
Fig. 1 is the structural representation of the utility model embodiment;
In figure: 1, tank body; 2, cover plate; 3, pumping pipe; 4, fluid inlet; 5, vapor pipe; 6, screen plate; 7, sterile air inlet pipe; 8, production well; 9, liquid-inlet pipe; 10, sealing member; 11, drawing handle; 12, projection.
Embodiment
The present embodiment relate to a kind of for animal cell culture can the culture tank of quick change liquid soon, as shown in fig. 1, it comprises the tank body 1 of inner hollow, top end opening, be provided with a cover plate 2 tank body 1 opening part is dismountable, cover plate 2 is a hollow structure inside, can adopt screw thread compact siro spinning technology between itself and tank body 1.In tank body 1, be provided with a screen plate 6 that can move up and down along tank body 1 inwall, screen plate 6 be provided with the mesh that aperture is 2.2 microns or 4 microns, can be used for the filtration to different animals cell culture fluid.Movablely on cover plate 2 be inserted with the pumping pipe 3 and vapor pipe 5 that bottom is connected with screen plate 6 respectively, the bottom import of pumping pipe 3, also namely the fluid inlet 4 of pumping pipe 3 is positioned at the top of screen plate 6, and is provided with valve in the exit, top of pumping pipe 3.The bottom import of vapor pipe 5 is positioned at the below of screen plate 6, is also provided with valve in the exit, top of vapor pipe 5.
Cover plate 2 is also provided with and the sterile air inlet pipe 7 be connected in tank body 1 and liquid-inlet pipe 9, in the present embodiment, sterile air inlet pipe 7 is directly connected with the empty internal of cover plate 2, then be provided with in the bottom of cover plate 2 and make cover plate 2 inside and the production well 8 be connected in tank body 1, thus make the sterile air entered via sterile air inlet pipe 7 can enter in tank body 1, and the sterile air entering cover plate 2 inside also makes cover plate 2 inside be in the higher barotropic state of a kind of relative ambient pressure, the positive seal to pumping pipe 3 and vapor pipe 5 and cover plate 2 junction can be realized.In sterile air inlet pipe 7 and liquid-inlet pipe 9, also valve is equipped with in the present embodiment, and at pumping pipe 3 be also equipped with sealing member 10 between vapor pipe 5 and cover plate 2, and sealing member 10 is for being arranged at the top of cover plate 2, its material can be rubber.
For making up and down more stable under cover plate 2 supports of pumping pipe 3 and vapor pipe 5 in the present embodiment, to ensure that the space between all edges of screen plate 6 and tank body 1 inwall all maintains in rational scope, the lower case of cover plate 2 is also provided with projection 12 in pumping pipe 3 and vapor pipe 5 place.What projection 12 axis that can be along pumping pipe 3 and vapor pipe 5 protruded from cover plate 2 lower case both sides has certain thickness bulge-structure.And for the ease of carrying out upper and lower push-and-pull via pumping pipe 3 and vapor pipe 5 pairs of screen plates 6, in the present embodiment, be also connected with a drawing handle 11 on the top of pumping pipe 3 and vapor pipe 5.
This be used for animal cell culture can soon quick change liquid cultivation filling in use, when needs carry out changing liquid, can pumping pipe 3 outlet connect on liquid absorbing pump, and make sterile air inlet pipe 7 connect upper sterile air pipeline, then the valve in pumping pipe 3 and sterile air inlet pipe 7 is opened respectively, promoting drawing handle 11 makes screen plate 6 move down along tank body 1 inwall, thus the substratum in tank body 1 in nutrient solution can be made to filter and the top of the screen plate 6 that arrives through filter plate 6, and enter in pumping pipe 3 through fluid inlet 4 and be finally drawn out of.Zooblast in nutrient solution is then retained in tank body 1 because of the stop of screen plate 6, owing to considerably increasing filtration area, thus can realize the raising greatly of changing liquid efficiency.
After in tank body 1, substratum exhausts, new substratum can be added in tank body 1 through liquid-inlet pipe 9, and in drawing liquid process or after drawing liquid, if have zooblast or other solid blockade screen plate 6 mesh, then can close pumping pipe 3 valve, and make screen plate 6 moves on nutrient solution liquid level, sterile air is entered in tank body 1, then screen plate 6 mesh can be washed open by the valve opened on vapor pipe 5 via sterile air.
Claims (4)
1. one kind for animal cell culture can the culture tank of quick change liquid soon, comprise inner hollow, the tank body of top end opening, it is characterized in that: be provided with a cover plate tank body opening part is dismountable, a screen plate that can move up and down along inner tank wall is provided with in tank body, movablely on described cover plate be inserted with the pumping pipe and vapor pipe that bottom is connected with screen plate respectively, the bottom import of described pumping pipe is positioned at the top of screen plate, valve is provided with in the exit, top of pumping pipe, the bottom import of described vapor pipe is positioned at the below of screen plate, valve is provided with in the exit, top of vapor pipe, described cover plate is provided with and the sterile air inlet pipe be connected in tank body and liquid-inlet pipe, described sterile air inlet pipe and liquid-inlet pipe are equipped with valve, sealing member is equipped with at pumping pipe with between vapor pipe and cover plate.
2. according to claim 1 for animal cell culture can the culture tank of quick change liquid soon, it is characterized in that: described cover plate is the shell structure of inner hollow, described sterile air inlet pipe is communicated in the inside of cover plate, be provided with the production well be connected in tank body in the bottom of cover plate, described sealing member is located at the top of cover plate.
3. according to claim 2 for animal cell culture can the culture tank of quick change liquid soon, it is characterized in that: be provided with the projection movement of pumping pipe and vapor pipe being carried out to guide support in the bottom of described cover plate.
4. according to claim 1 for animal cell culture can the culture tank of quick change liquid soon, it is characterized in that: be connected with drawing handle on the top of pumping pipe and vapor pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420527637.1U CN204058483U (en) | 2014-09-15 | 2014-09-15 | For animal cell culture can the culture tank of quick change liquid soon |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420527637.1U CN204058483U (en) | 2014-09-15 | 2014-09-15 | For animal cell culture can the culture tank of quick change liquid soon |
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| Publication Number | Publication Date |
|---|---|
| CN204058483U true CN204058483U (en) | 2014-12-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420527637.1U Expired - Fee Related CN204058483U (en) | 2014-09-15 | 2014-09-15 | For animal cell culture can the culture tank of quick change liquid soon |
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| CN (1) | CN204058483U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104988063A (en) * | 2015-06-19 | 2015-10-21 | 湖南中医药大学 | Cell culture dish capable of preventing cell contamination and application of cell culture dish |
| CN111304086A (en) * | 2019-12-05 | 2020-06-19 | 武汉百泰康基因技术有限公司 | Cell culture negative pressure liquid change operating system |
| CN112662556A (en) * | 2021-01-07 | 2021-04-16 | 赵福新 | Automatic cell culture device capable of reducing cell pollution |
-
2014
- 2014-09-15 CN CN201420527637.1U patent/CN204058483U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104988063A (en) * | 2015-06-19 | 2015-10-21 | 湖南中医药大学 | Cell culture dish capable of preventing cell contamination and application of cell culture dish |
| CN104988063B (en) * | 2015-06-19 | 2017-11-07 | 湖南中医药大学 | A kind of Tissue Culture Dish for preventing cell contamination and its application |
| CN111304086A (en) * | 2019-12-05 | 2020-06-19 | 武汉百泰康基因技术有限公司 | Cell culture negative pressure liquid change operating system |
| CN112662556A (en) * | 2021-01-07 | 2021-04-16 | 赵福新 | Automatic cell culture device capable of reducing cell pollution |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141231 Termination date: 20160915 |
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| CF01 | Termination of patent right due to non-payment of annual fee |