CN112662556A - Automatic cell culture device capable of reducing cell pollution - Google Patents
Automatic cell culture device capable of reducing cell pollution Download PDFInfo
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- CN112662556A CN112662556A CN202110020470.4A CN202110020470A CN112662556A CN 112662556 A CN112662556 A CN 112662556A CN 202110020470 A CN202110020470 A CN 202110020470A CN 112662556 A CN112662556 A CN 112662556A
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Abstract
The invention relates to the technical field of cell culture devices, and discloses an automatic cell culture device capable of reducing cell pollution, which comprises a tank body, a sealing cover component and a culture component, wherein the front surface of the tank body is movably connected with a closed glass door, through the matching use of the culture component and each structure in the sealing cover component, the residual air in the tank body can be directly discharged out of the tank body by pressing a top cover, then, clean gas suitable for cell culture and growth can be directly introduced through the outside to reduce the pollution of cells in culture, a one-way valve is arranged in a gas guide pipe, the gas can be effectively prevented from flowing back from the gas guide pipe when the gas in the tank body is extruded, through the movably connected closed glass door arranged outside the tank body, a user can conveniently open and close the tank body at any time, and can conveniently observe the cell culture condition in the tank body directly through the closed glass door after the closed glass door is closed, the device has simple integral structure and convenient repeated use.
Description
Technical Field
The invention relates to the technical field of cell culture devices, in particular to an automatic cell culture device capable of reducing cell pollution.
Background
Cell culture generally refers to a method of simulating in vitro an environment of in vivo sterility, suitable temperature, pH value and certain nutritional conditions, allowing cells to grow, reproduce and maintain the main structure and function. In the conventional cell culture device, cells to be cultured are usually placed in a sterile closed glass utensil for growth, but once the device is repeatedly used, residual gas originally cultured is retained in a bottle cavity, so that the subsequent culture effect is influenced, workers generally clean the utensil or directly replace a new utensil for re-culture, so that the time and the labor are consumed, and the cell culture cost is increased, and therefore, the cell automatic culture device capable of reducing cell pollution is provided to solve the problems.
Disclosure of Invention
In order to achieve the purposes of effectively reducing the pollution in the culture device and reusing the culture device, the invention provides the following technical scheme: the utility model provides a cell automatic culture device who reduces cell pollution, includes a jar body, closing cap subassembly and cultivates the subassembly, the front swing joint of jar body has closed glass door, the bottom inner wall fixed mounting of jar body has the air duct, the outer wall fixed mounting of air duct has the check valve, the inner wall fixed mounting of jar body has the spacing ring, the inner wall and the closing cap subassembly outer wall swing joint of jar body, the inner wall of jar body and the outer wall swing joint of cultivateing the subassembly.
As optimization, cultivate the subassembly including cultivateing the frame, the equal fixed mounting in top and the bottom inner wall of cultivation frame has ventilative frame plate, two the equal swing joint in top of ventilative frame plate has the culture dish, the top inner wall fixed mounting who cultivates the frame has the bracing piece that quantity is four, four relative one side fixed mounting of bracing piece has the supporting seat, the top fixed mounting of supporting seat has the kicking block, the inner wall fixed mounting of kicking block has first spring, the left side fixed mounting of first spring has first lug.
As an optimization, the sealing cover assembly comprises a top cover, a plurality of telescopic rods are fixedly mounted on the left side and the right side of the bottom of the top cover, pulleys are fixedly mounted on the left side of the top cover, the left inner wall of the top cover is movably connected with two first fan plates, the tops of the opposite sides of the two first fan plates are fixedly mounted with stoppers, first elastic ropes are fixedly mounted on the bottoms of the opposite sides of the two first fan plates, a support plate is fixedly mounted on the left inner wall of the top cover, a rotating rod is fixedly mounted on the right side of the support plate, a second fan plate is movably connected with the right inner wall of the rotating rod, a second elastic rope is fixedly mounted on the bottom of the second fan plate, a second spring is fixedly mounted on the right inner wall of the second fan plate, a second convex block is fixedly mounted on the right side of the second spring, and an air bag is fixedly, the top left side fixed mounting of gasbag has the gas-supply pipe, the bottom fixed mounting of gas-supply pipe has the air pressure pole.
As the optimization, cultivate the bottom of frame and the bottom inner wall swing joint of the jar body, cultivate the outer wall of frame and the inner wall swing joint of spacing ring, cultivate the bottom of frame and seted up the inlet port, the right side of air duct runs through the left side outer wall of the jar body and extends to the bottom inner wall of cultivateing the frame through this inlet port for cultivate the whole separation between the frame and the jar body, and cultivate the frame bottom and set up the inlet port, convenient follow-up can be directly from the air duct to cultivate leading-in pure gas in the frame.
As optimization, the number of the first springs and the first convex blocks is four, one side of each first spring opposite to the inner wall of the top block is fixedly connected with the inner wall of the top block, one side of each first spring opposite to the other side of each first convex block is fixedly connected with one side of each first convex block opposite to the other side of each first spring, the four first convex blocks are arranged to facilitate direct clamping with the top cover in a follow-up mode, and the top cover can be fixed after being pressed downwards.
As optimization, the bottoms of the multiple telescopic rods are respectively fixedly connected with the left side and the right side of the top of the limiting ring, and the outer walls of the two multiple telescopic rods are movably connected with supporting springs, so that the multiple telescopic rods are not subjected to external force, and can provide supporting force for the top cover through the supporting springs.
As optimization, the quantity of pulley is total four, four relative one sides of pulley respectively with the bottom left side of top cap, right side, front and back fixed connection, the top inner wall of the jar body seted up with the spout of four pulley looks adaptations for the top cap can be more steady at the in-process that moves down.
As the optimization, the slot with kicking block looks adaptation is seted up to the inner wall of top cap, the gas-supply pipe all has four with the quantity of air pressure bar, the relative one side of four gas-supply pipes respectively with the top left side of gasbag, the right side, front and back inner wall fixed connection, the inside one side bottom of the back of the body that carries on the back of the body of four gas-supply pipes runs through the top of four air pressure bars respectively and extends to the inner wall of four air pressure bars, when making the top cap push down to cultivate the frame top, the kicking block can extend to the slot of top cap inner wall in, thereby can extrude the gasbag, make the inside atmospheric pressure of gasbag can transport respectively to four air pressure bars through four gas-supply.
As the optimization, the fixed slot that quantity is four is seted up to the inner wall of top cap, four fixed slots respectively with four first lug looks adaptations, the quantity of second lug is total four, the air pressure pole comprises interior pole and outer pole, the slot with the interior pole looks adaptations of four air pressure poles is all seted up to the inner wall of four second lugs, make the top cap push down the back, the fixed slot of top cap inner wall can carry out the joint with four first lugs of kicking block inner wall, thereby it is fixed with the top cap, and can extend to in the second lug after four air pressure poles receive atmospheric pressure to extend, thereby it is fixed with the second fan board, prevent that the gas that follow-up lets in from the through-hole of top cap is discharged.
The invention has the beneficial effects that: the culture component and the sealing cover component are arranged, and the culture component and each structure in the sealing cover component are matched for use, so that cells to be cultured are placed in the tank body through the culture rack, the residual air in the tank body can be directly discharged out of the tank body by pressing down the top cover, then clean gas suitable for cell culture growth can be directly introduced from the outside to reduce the pollution of cells in culture, and a one-way valve is arranged in the gas guide pipe, can effectively prevent the gas from flowing back from the gas guide tube when the gas in the tank body is extruded, and the closed glass door is movably connected with the outside of the tank body, so that the glass door can be opened and closed conveniently by a user at any time, and the glass door can be closed conveniently, the user can directly observe the cell culture condition in the tank body through the closed glass door, and the device has a simple integral structure and is convenient to reuse.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a sectional view of a can body structure according to the present invention;
FIG. 3 is an enlarged view of the structure at A in FIG. 2 according to the present invention;
FIG. 4 is an enlarged fragmentary view of the closure assembly of the present invention;
FIG. 5 is an enlarged view of the structure at B in FIG. 4 according to the present invention.
In the figure: 1. a tank body; 2. closing the glass door; 3. an air duct; 4. a one-way valve; 5. a closure assembly; 6. a limiting ring; 60. a culture assembly; 61. a culture shelf; 62. a breathable frame plate; 63. a culture dish; 64. a support bar; 65. a supporting seat; 66. a top block; 67. a first spring; 68. a first bump; 501. a top cover; 502. a multi-section telescopic rod; 503. a pulley; 504. a first fan plate; 505. a limiting block; 506. a first elastic cord; 507. a support plate; 508. a rotating rod; 509. a second fan plate; 510. a second elastic cord; 511. a second spring; 512. a second bump; 513. an air bag; 514. a gas delivery pipe; 515. a pneumatic rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-2, an automatic cell culture device for reducing cell contamination includes a tank 1, a cover assembly 5 and a culture assembly 60, the front of the tank 1 is movably connected with a closed glass door 2, the inner wall of the bottom of the tank 1 is fixedly provided with an air duct 3, the outer wall of the air duct 3 is fixedly provided with a one-way valve 4, the inner wall of the tank 1 is fixedly provided with a limit ring 6, the inner wall of the tank 1 is movably connected with the outer wall of the cover assembly 5, and the inner wall of the tank 1 is movably connected with the outer wall of the culture assembly 60.
Referring to fig. 2-3, the cultivation assembly 60 includes a cultivation frame 61, the bottom of the cultivation frame 61 is movably connected to the inner wall of the bottom of the tank 1, the outer wall of the cultivation frame 61 is movably connected to the inner wall of the limiting ring 6, an air inlet hole is opened at the bottom of the cultivation frame 61, the right side of the air duct 3 penetrates through the outer wall of the left side of the tank 1 and extends to the inner wall of the bottom of the cultivation frame 61 through the air inlet hole, so that the whole cultivation frame 61 can be separated from the tank 1, an air inlet hole is opened at the bottom of the cultivation frame 61, so that purified gas can be directly introduced into the cultivation frame 61 from the air duct 3 in the subsequent process, air permeable frame plates 62 are fixedly installed on the top and the inner wall of the bottom of the cultivation frame 61, cultivation dishes 63 are movably connected to the tops of the two air permeable frame plates 62, four support rods, top fixed mounting of supporting seat 65 has kicking block 66, the inner wall fixed mounting of kicking block 66 has first spring 67, the left side fixed mounting of first spring 67 has first lug 68, first spring 67 all has four with the quantity of first lug 68, the relative one side of four first springs 67 and the inner wall fixed connection of kicking block 66, the one side of carrying on the back of the body of four first springs 67 respectively with the one side fixed connection that carries on the back of the body of four first lugs 68, set up the convenient follow-up direct joint with top cap 501 of four first lugs 68, can be fixed after making top cap 501 push down.
Referring to fig. 2 and fig. 4-5, the capping assembly 5 includes a top cap 501, the bottoms of two multi-section telescopic rods 502 are respectively fixedly connected to the left and right sides of the top of the limiting ring 6, the outer walls of the two multi-section telescopic rods 502 are movably connected to supporting springs, so that the multi-section telescopic rods 502 are not subjected to an external force, the multi-section telescopic rods 502 can provide a supporting force to the top cap 501 through the supporting springs, the left and right sides of the bottom of the top cap 501 are respectively and fixedly provided with the multi-section telescopic rods 502, the left side of the top cap 501 is fixedly provided with four pulleys 503, opposite sides of the four pulleys 503 are respectively and fixedly connected to the left side, the right side, the front side and the back side of the bottom of the top cap 501, the inner wall of the top of the tank body 1 is provided with sliding grooves adapted to the four pulleys 503, so that the top cap, the top of the opposite side of each of the two first fan plates 504 is fixedly provided with a limiting block 505, the bottom of the opposite side of each of the two first fan plates 504 is fixedly provided with a first elastic rope 506, the left inner wall of the top cover 501 is fixedly provided with a supporting plate 507, the right side of the supporting plate 507 is fixedly provided with a rotating rod 508, the right inner wall of the rotating rod 508 is movably connected with a second fan plate 509, the bottom of the second fan plate 509 is fixedly provided with a second elastic rope 510, the right inner wall of the second fan plate 509 is fixedly provided with a second spring 511, the right side of the second spring 511 is fixedly provided with a second lug 512, the inner wall of the top cover 501 is fixedly provided with an air bag 513, the left side of the top of the air bag 513 is fixedly provided with an air pipe 514, the bottom of the air pipe 514 is fixedly provided with an air pressure rod 515, the inner wall of the top cover 501 is provided with a slot matched with the top block 66, the air pipe 514 and, The inner walls of the right side, the front side and the back side are fixedly connected, the bottoms of the opposite sides of the four air pipes 514 respectively penetrate through the tops of the four air pressure rods 515 and extend to the inner walls of the four air pressure rods 515, when the top cover 501 is pressed down to the top of the culture shelf 61, the top block 66 can extend into the slot on the inner wall of the top cover 501, so as to extrude the air bag 513, the air pressure inside the air bag 513 can be respectively transported into the four air pressure rods 515 through the four air pipes 514, the extension of the air pressure rods 515 is controlled, the inner wall of the top cover 501 is provided with four fixing slots, the four fixing slots are respectively matched with the four first lugs 68, the number of the second lugs 512 is four, the air pressure rods 515 are composed of inner rods and outer rods, the slots matched with the inner rods of the four air pressure rods 515 are respectively arranged on the inner walls of the four second lugs 512, after the top cover 501 is pressed down, the fixing slots on, thereby fixing the top cover 501, and when the four air pressure rods 515 are expanded by the air pressure, they can be extended into the second protrusions 512, thereby fixing the second fan plate 509 and preventing the subsequently introduced air from being discharged from the through hole of the top cover 501.
In use, referring to fig. 1-5, a user can open the closed glass door 2, remove the culture rack 61 from the tank 1, pour the cell solution to be cultured into the two culture dishes 63 and place the culture rack on the air permeable shelf 62 again, move the culture rack 61 back into the tank 1, align the air inlet hole at the bottom of the culture rack 61 with the air duct 3, close the closed glass door 2 to ensure the sealing of the tank 1, and manually press the top cover 501 downwards, wherein the air in the tank 1 including the culture rack 61 flows towards the four through holes of the top cover 501 along with the downward pressing of the top cover 501, so that the internal air starts to press the four second panels 509, and the second panels 509 are pressed to apply a force on the second bump 512 corresponding to the inner wall of each second panel 509, so that the second bumps 512 shrink into the second panels 509, at this time, the second fan plate 509 loses the limiting force, so that the second fan plate 509 can rotate around the corresponding rotating rod 508, the gas in the tank body 1 is discharged from the second fan plate 509, then the two first fan plates 504 in each through hole are extruded, the first fan plates 504 can be smoothly opened, the top cover 501 continuously moves downwards, finally, the air in the tank body 1 and the culture rack 61 is discharged along with the through holes, after the gas is exhausted, the first fan plates 504 and the second fan plates 509 lose the acting force, so that the first elastic ropes 506 and the second elastic ropes 510 are pulled to reset, each second bump 512 can be clamped into the slot in the top cover 501 again, when the top cover 501 moves to the top position of the culture rack 61, the top block 66 above the culture rack 61 is inserted into the slot on the inner wall of the top cover 501 rightly, so as to extrude the air bag 513, after the air bag 513 is extruded, the internal air pressure is respectively transported to the corresponding four air pressure rods 515 through the four air pipes 514, thereby extend the interior pole of four pneumatic poles 515 and insert in every second lug 512, it is fixed with every second fan board 509, the four first lugs 68 of kicking block 66 inner wall also can insert in the draw-in groove that the top cap 501 inner wall corresponds, it is fixed between top cap 501 and the culture rack 61 for form airtight environment in the culture rack 61, finally, the user can accomplish the operation in letting in the clean gas that suitable cell culture through air duct 3 in to culture rack 61.
To sum up, a user can open the closed glass door 2, take out the culture rack 61 inside the tank 1 from the tank 1, pour the cell solution to be cultured into the two culture dishes 63 and place the cell solution on the gas permeable frame plate 62 again, then move the culture rack 61 back to the inside of the tank 1, place the gas inlet hole at the bottom of the culture rack 61 in alignment with the gas guide tube 3, close the closed glass door 2 to ensure the sealing of the tank 1, and manually press the top cover 501 downwards at this time, the air inside the tank 1 including the culture rack 61 flows towards the four through holes formed in the top cover 501 along with the downward pressing of the top cover 501, so that the internal gas starts to press the four second panels 509, and after the second panels are pressed, the acting force is applied to the second bump 512 corresponding to the inner wall of each second panel 509, so that the second bump 512 shrinks into the second panel 509, and at this time, the second panels 509 lose the restraining force, so that the gas in the tank body 1 can be discharged from the second fan plate 509 by rotating with the corresponding rotating rod 508 as an axis, then two first fan plates 504 in each through hole are extruded, the first fan plates 504 can be smoothly opened, the air in the tank body 1 and the culture rack 61 is finally discharged along with the continuous downward movement of the top cover 501, the first fan plates 504 and the second fan plates 509 lose force after the gas is discharged, so that the first fan plates and the second fan plates 509 are pulled and reset by the corresponding first elastic ropes 506 and the corresponding second elastic ropes 510, each second lug 512 can be clamped into the slot in the top cover 501 again, when the top cover 501 moves to the top position of the culture rack 61, the top block 66 above the culture rack 61 is also inserted into the slot in the inner wall of the top cover 501, so as to extrude the air bag 513, the air bag 513 is extruded, and then the internal air pressure is transported to the corresponding four rods 515 through the four air pipes 514, so that the inner rods in the four air pressure rods 515 are extended and inserted into each second lug 512, fix every second fan plate 509, the four first lugs 68 of kicking block 66 inner wall also can insert the draw-in groove that the top cap 501 inner wall corresponds in, with fixed between top cap 501 and the cultivation frame 61 for form airtight environment in the cultivation frame 61, finally, the user can accomplish the operation in letting in the clean gas that suits the cell culture through air duct 3 in to cultivation frame 61.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.
Claims (9)
1. The utility model provides a reduce cell contamination's automatic culture device of cell, includes jar body (1), closing cap subassembly (5) and cultivates subassembly (60), its characterized in that: the positive swing joint of the jar body (1) has closed glass door (2), the bottom inner wall fixed mounting of the jar body (1) has air duct (3), the outer wall fixed mounting of air duct (3) has check valve (4), the inner wall fixed mounting of the jar body (1) has spacing ring (6), the inner wall and the closing cap subassembly (5) outer wall swing joint of the jar body (1), the inner wall of the jar body (1) and the outer wall swing joint of cultivateing subassembly (60).
2. The automatic cell culture apparatus according to claim 1, wherein: cultivate subassembly (60) including cultivateing frame (61), the top and the equal fixed mounting in bottom inner wall of cultivateing frame (61) have ventilative frame plate (62), two the equal swing joint in top of ventilative frame plate (62) has culture dish (63), the top inner wall fixed mounting who cultivates frame (61) has bracing piece (64) that quantity is four, four the relative one side fixed mounting of bracing piece (64) has supporting seat (65), the top fixed mounting of supporting seat (65) has kicking block (66), the inner wall fixed mounting of kicking block (66) has first spring (67), the left side fixed mounting of first spring (67) has first lug (68).
3. The automatic cell culture apparatus according to claim 2, wherein: the sealing cover component (5) comprises a top cover (501), a plurality of sections of telescopic rods (502) are fixedly mounted on the left side and the right side of the bottom of the top cover (501), pulleys (503) are fixedly mounted on the left side of the top cover (501), a first sector plate (504) with the number of two is movably connected to the inner wall of the left side of the top cover (501), a limiting block (505) is fixedly mounted on the top of one opposite side of the first sector plate (504), a first elastic rope (506) is fixedly mounted on the bottom of one opposite side of the first sector plate (504), a supporting plate (507) is fixedly mounted on the inner wall of the left side of the top cover (501), a rotating rod (508) is fixedly mounted on the right side of the supporting plate (507), a second sector plate (509) is movably connected to the inner wall of the right side of the rotating rod (508), and a second elastic rope (510) is fixedly, the right side inner wall fixed mounting of second fan board (509) has second spring (511), the right side fixed mounting of second spring (511) has second lug (512), the inner wall fixed mounting of top cap (501) has gasbag (513), the top left side fixed mounting of gasbag (513) has air-supply pipe (514), the bottom fixed mounting of air-supply pipe (514) has air pressure lever (515).
4. The automatic cell culture apparatus according to claim 2, wherein: the bottom of the cultivation frame (61) is movably connected with the inner wall of the bottom of the tank body (1), the outer wall of the cultivation frame (61) is movably connected with the inner wall of the limiting ring (6), an air inlet hole is formed in the bottom of the cultivation frame (61), and the right side of the air guide pipe (3) penetrates through the outer wall of the left side of the tank body (1) and extends to the inner wall of the bottom of the cultivation frame (61) through the air inlet hole.
5. The automatic cell culture apparatus according to claim 2, wherein: the number of the first springs (67) and the number of the first convex blocks (68) are four, the opposite sides of the four first springs (67) are fixedly connected with the inner wall of the top block (66), and the back sides of the four first springs (67) are fixedly connected with the back sides of the four first convex blocks (68) respectively.
6. The automatic cell culture apparatus according to claim 3, wherein: the bottoms of the two multi-section telescopic rods (502) are respectively fixedly connected with the left side and the right side of the top of the limiting ring (6), and the outer walls of the two multi-section telescopic rods (502) are movably connected with supporting springs.
7. The automatic cell culture apparatus according to claim 3, wherein: the quantity of pulley (503) is four in total, four pulley (503) relative one side respectively with the bottom left side of top cap (501), right side, front and back fixed connection, the top inner wall of jar body (1) seted up with the spout of four pulley (503) looks adaptations.
8. The automatic cell culture apparatus according to claim 3, wherein: the slot with kicking block (66) looks adaptation is seted up to the inner wall of top cap (501), and the quantity of gas-supply pipe (514) and atmospheric pressure pole (515) all has four, the relative one side of four gas-supply pipes (514) respectively with the top left side of gasbag (513), right side, front and back inner wall fixed connection, the inside wall that runs through the top of four atmospheric pressure poles (515) and extend to four atmospheric pressure poles (515) respectively in the back of the body one side bottom of carrying on the back of the body of four gas-supply pipes (514).
9. The automatic cell culture apparatus according to claim 3, wherein: the inner wall of the top cover (501) is provided with four fixing grooves, the four fixing grooves are respectively matched with the four first convex blocks (68), the number of the second convex blocks (512) is four, the air pressure rods (515) are composed of inner rods and outer rods, and the inner walls of the four second convex blocks (512) are provided with slots matched with the inner rods of the four air pressure rods (515).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110020470.4A CN112662556B (en) | 2021-01-07 | 2021-01-07 | Automatic cell culture device capable of reducing cell pollution |
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| CN202110020470.4A CN112662556B (en) | 2021-01-07 | 2021-01-07 | Automatic cell culture device capable of reducing cell pollution |
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| KR100879597B1 (en) * | 2006-12-13 | 2009-01-21 | 주식회사 바이오트론 | Device for Cell Manipulation and Cultivation in a Closed Environment |
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| CN112662556B (en) | 2022-05-17 |
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