CN209778890U - Multifunctional cell culture bottle - Google Patents

Multifunctional cell culture bottle Download PDF

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Publication number
CN209778890U
CN209778890U CN201822257071.6U CN201822257071U CN209778890U CN 209778890 U CN209778890 U CN 209778890U CN 201822257071 U CN201822257071 U CN 201822257071U CN 209778890 U CN209778890 U CN 209778890U
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CN
China
Prior art keywords
bottle
bottle body
cell culture
observation part
iron sheet
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Active
Application number
CN201822257071.6U
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Chinese (zh)
Inventor
甘露
张维
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Shenzhen Light Life Engineering Technology Co Ltd
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Shenzhen Light Life Engineering Technology Co Ltd
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Priority to CN201822257071.6U priority Critical patent/CN209778890U/en
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Publication of CN209778890U publication Critical patent/CN209778890U/en
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Abstract

The utility model relates to a multifunctional cell culture bottle, which comprises a bottle cap and a bottle body which is vertically arranged, wherein the bottle cap can be detachably covered on the top of the bottle body, a transparent observation part is arranged on one side wall of the bottle body, and the observation part is vertically arranged in a rectangular shape; a moving rod is arranged in the bottle body corresponding to the observation part, and an iron sheet is wrapped in the moving rod; a permanent magnetic stripe is arranged outside the bottle body corresponding to the observation part; the permanent magnetic stripe magnet attracts an iron sheet in the moving rod, and the moving rod is driven to move in the bottle body through the iron sheet. Compared with the prior art, the utility model discloses permanent magnetism magnetic stripe magnetism is inhaled iron sheet in the carriage release lever drives through the iron sheet the carriage release lever is in remove in the bottle to make the culture solution in the bottle flow, promote the even cultivation of cell in the bottle, can effectively ensure testing result's accuracy.

Description

Multifunctional cell culture bottle
Technical Field
The utility model relates to a cell culture technical field, in particular to multifunctional cell culture bottle.
Background
Cell culture refers to the growth of cells in vitro under artificially provided conditions, and the cells grown in the culture process do not differentiate into tissues any more, which is the basis and important production means in biotechnology. The purpose of this is to harvest the cells themselves or to obtain cellular metabolites.
The cell culture is roughly divided into adherent cell culture and suspension cell culture according to the characteristics of different types of cells. During the process of adherent cell culture, pancreatin is needed for digestion, and after a period of time, the cells tightly attached to the wall of the culture flask are separated to form a suspension state.
When the cells are cultured, because the mobility of the cell culture solution is poor, and the gas in the bottle body and the like cannot be uniformly distributed in the bottle body and the horizontal section at some time, the cell concentration and the like of the cell culture solution in the horizontal section and the cell culture solution in the bottle body can be caused to be different, at this time, if an operator observes the cells in the horizontal section and then deduces the growth condition and the like of the cells in the bottle body, the detection result cannot completely and correctly reflect the condition of the cells in the bottle, the detection result cannot be accurate, the correctness of the results of scientific research and the like cannot be ensured, great disadvantages are brought to operations such as inspection and the like, meanwhile, the accuracy of cell culture and the like cannot be ensured, the correct use of the cultured cells or cell metabolites cannot be ensured, and the using effect cannot be ensured.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a multi-functional cell culture bottle, the technical problem that solve is: the mobility of the cell culture solution is poor, the detection result is inaccurate, great disadvantages are brought to operations such as detection, and meanwhile, the accuracy of cell culture and the like cannot be guaranteed.
The utility model provides an above-mentioned technical problem's technical scheme as follows: a multifunctional cell culture bottle comprises a bottle cap and a bottle body which is vertically arranged, wherein the bottle cap is detachably used for sealing the top of the bottle body, a transparent observation part is arranged on one side wall of the bottle body, and the observation part is vertically arranged in a rectangular shape; a moving rod is arranged in the bottle body corresponding to the observation part, and an iron sheet is wrapped in the moving rod; a permanent magnetic stripe is arranged outside the bottle body corresponding to the observation part; the permanent magnetic stripe magnet attracts an iron sheet in the moving rod, and the moving rod is driven to move in the bottle body through the iron sheet.
On the basis of the technical scheme, the utility model discloses can also do following improvement.
Further, the upper end of the observation part extends to the upper part of the bottle body, and the lower part of the observation part extends to the bottom of the bottle body; the observation portion is made of glass.
Further, the bottom of the bottle body is communicated with a liquid outlet pipe which is horizontally arranged, a one-way valve is arranged at the position where the liquid outlet pipe is communicated with the bottle body, and the liquid outlet pipe is controlled to be opened and closed through the one-way valve.
Further, a semi-permeable membrane is arranged at the bottle mouth at the upper end of the bottle body, and the semi-permeable membrane covers the bottle mouth of the bottle body; the bottle cap covers the semi-permeable membrane.
Furthermore, the semipermeable membrane is made of polytetrafluoroethylene or silicon rubber.
Further, a lantern ring is sleeved at the upper end bottleneck of the bottle body and is connected with the permanent magnetic stripe through a connecting rod.
The utility model has the advantages that: the permanent magnetism magnetic stripe magnetism is inhaled iron sheet in the carriage release lever drives through the iron sheet the carriage release lever is in remove in the bottle to make the culture solution in the bottle flow, promote the even cultivation of cell in the bottle, can effectively ensure the accuracy of testing result.
Drawings
FIG. 1 is a front view of a multi-functional cell culture flask according to the present invention;
Fig. 2 is a schematic diagram of a side view structure of the multifunctional cell culture bottle of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1. The bottle comprises a bottle cap, 2, a bottle body, 3, an observation part, 5, a permanent magnetic strip, 6, a moving rod, 7, a liquid outlet pipe, 8, a one-way valve, 9 and a semipermeable membrane.
Detailed Description
The principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.
As shown in fig. 1 and 2, a multifunctional cell culture bottle comprises a bottle cap 1 and a bottle body 2 which is vertically arranged, wherein the bottle cap 1 detachably covers the top of the bottle body 2, a transparent observation part 3 is arranged on one side wall of the bottle body 2, and the observation part 3 is vertically arranged in a rectangular shape; a moving rod 6 is arranged in the bottle body 2 corresponding to the observation part 3, and an iron sheet is wrapped in the moving rod 6; a permanent magnetic stripe 5 is arranged outside the bottle body 2 corresponding to the observation part 3; the permanent magnetic stripe 5 magnetically attracts an iron sheet in the movable rod 6, and the movable rod 6 is driven by the iron sheet to move in the bottle body 2. Wherein the observation part 3 and the moving rod 6 are made of glass.
In the above embodiment, the upper end of the observation part 3 extends to the upper part of the bottle body 2, and the lower part of the observation part 3 extends to the bottom of the bottle body 2; the observation portion 3 is made of glass.
In the above embodiment, the bottom of the bottle body 2 is communicated with a liquid outlet pipe 7 which is horizontally arranged, a one-way valve 8 is arranged at the communication position of the liquid outlet pipe 7 and the bottle body 2, and the liquid outlet pipe 7 is controlled to be opened and closed by the one-way valve 8.
In the above embodiment, a semi-permeable membrane 9 is arranged at the bottle mouth at the upper end of the bottle body 2, and the semi-permeable membrane 9 covers the bottle mouth of the bottle body 2; the closure 1 closes the semi-permeable membrane 9. The semipermeable membrane 9 is breathable, is beneficial to cell ventilation, meets the requirements of oxygen and carbon dioxide, is beneficial to cell growth, has quick cell proliferation and good culture effect, and meanwhile, the semipermeable membrane 9 can effectively prevent external microorganisms from entering the bottle body, so that the bottle is pollution-free, the growth and the propagation of cells are in the optimal state, the cell activity is high,
In the above embodiment, the semi-permeable membrane 9 is made of teflon or silicone rubber.
in the above embodiment, a lantern ring is sleeved at the upper end bottleneck of the bottle body 2, and the lantern ring is connected with the permanent magnetic stripe 5 through a connecting rod. The lantern ring and the connecting rod can be connected with the permanent magnetic strip 5, so that the permanent magnetic strip 5 is not easy to lose, and the convenience in operation of the permanent magnetic strip 5 can be improved.
In the embodiment, cells are cultured in the bottle body 2, in the process of culturing the cells, the permanent magnetic stripe 5 is moved, the permanent magnetic stripe 5 magnetically attracts an iron sheet in the movable rod 6, and the movable rod 6 is driven to move in the bottle body 2 through the iron sheet, so that the culture solution in the bottle body 2 flows, the cells in the bottle body 2 are promoted to be uniformly cultured, and the gas in the bottle body 2 can be uniformly and uniformly distributed in the bottle body 2 and the horizontal section; simultaneously observation portion 3 also is convenient for observe the cell cultivation situation of different horizontal segments in bottle 2, and the cell after cultivating is derived through drain pipe 7, detects, can effectively ensure the accuracy of testing result.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims (6)

1. a multi-functional cell culture bottle, includes bottle lid (1) and vertical arrangement's bottle (2), bottle lid (1) detachable closing cap the top of bottle (2), its characterized in that: a transparent observation part (3) is arranged on one side wall of the bottle body (2), and the observation part (3) is vertically arranged in a rectangular shape; a movable rod (6) is arranged in the bottle body (2) corresponding to the observation part (3), and an iron sheet is wrapped in the movable rod (6); a permanent magnetic stripe (5) is arranged outside the bottle body (2) corresponding to the observation part (3); the permanent magnetic stripe (5) magnetically attracts an iron sheet in the movable rod (6), and the movable rod (6) is driven by the iron sheet to move in the bottle body (2).
2. The multifunctional cell culture flask according to claim 1, wherein: the upper end of the observation part (3) extends to the upper part of the bottle body (2), and the lower part of the observation part (3) extends to the bottom of the bottle body (2); the observation part (3) is made of glass.
3. The multifunctional cell culture flask according to claim 1, wherein: the bottom of bottle (2) communicates drain pipe (7) that have the horizontal arrangement, drain pipe (7) with bottle (2) intercommunication department is provided with check valve (8), controls through check valve (8) drain pipe (7) are opened and are closed.
4. The multifunctional cell culture flask according to claim 1, wherein: a semi-permeable membrane (9) is arranged at the bottleneck at the upper end of the bottle body (2), and the semi-permeable membrane (9) covers the bottleneck of the bottle body (2); the bottle cap (1) covers the semi-permeable membrane (9).
5. The multifunctional cell culture flask according to claim 4, wherein: the semipermeable membrane (9) is made of polytetrafluoroethylene or silicon rubber.
6. the multifunctional cell culture flask according to claim 1, wherein: a lantern ring is sleeved at the upper end bottleneck of the bottle body (2), and the lantern ring is connected with the permanent magnetic stripe (5) through a connecting rod.
CN201822257071.6U 2018-12-30 2018-12-30 Multifunctional cell culture bottle Active CN209778890U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201822257071.6U CN209778890U (en) 2018-12-30 2018-12-30 Multifunctional cell culture bottle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201822257071.6U CN209778890U (en) 2018-12-30 2018-12-30 Multifunctional cell culture bottle

Publications (1)

Publication Number Publication Date
CN209778890U true CN209778890U (en) 2019-12-13

Family

ID=68791264

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201822257071.6U Active CN209778890U (en) 2018-12-30 2018-12-30 Multifunctional cell culture bottle

Country Status (1)

Country Link
CN (1) CN209778890U (en)

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