CN209797987U - Culture bottle for culturing DC-CIK cells - Google Patents
Culture bottle for culturing DC-CIK cells Download PDFInfo
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- CN209797987U CN209797987U CN201822107053.XU CN201822107053U CN209797987U CN 209797987 U CN209797987 U CN 209797987U CN 201822107053 U CN201822107053 U CN 201822107053U CN 209797987 U CN209797987 U CN 209797987U
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Abstract
The utility model discloses a cultivate DC-CIK blake bottle for cell, including bottle and ventilative hydrophobic bottle lid, the bottle includes horizontal part and rake, the rake cooperation of bottle lid and bottle, fixedly connected with horizontal baffle in the bottle, the horizontal part of bottle is bottle horizontal part I and bottle horizontal part II, the lateral wall fixedly connected with slope baffle of horizontal baffle, there is the interval between the slope baffle tilt up and its one end that is close to the bottle lid and the bottom of bottle lid, and the mixed passage of interval formation bottle horizontal part I and bottle horizontal part II intercommunication, the inner wall fixedly connected with connecting block of bottle, the interior division of connecting block has the cavity, the inner wall swing joint of cavity has the movable block, circular through-hole has been seted up to the upper surface of movable block. The utility model discloses the culture liquid that can not make on the bottle horizontal part I filling drips at bottle horizontal part II, and then has ensured that cell culture can succeed, has improved cell culture's success rate, also can improve the bottle and place for stability.
Description
Technical Field
The utility model relates to a cell culture technical field especially relates to a cultivate DC-CIK blake bottle for cell.
Background
The dendritic cells can identify and treat leukemia cells and transmit related signals of the leukemia cells to the CIK cells, so that the CIK cells are specially used for killing the leukemia cells, and the treatment of leukemia by the DC-CIK cells is a milestone for treating leukemia by using a cell biological therapy, so that the in-vitro DC-CIK cell combined culture technology is produced. At present, the scheme for culturing the DC-CIK cells is to separately culture the DC cells and the CIK cells by using a plurality of culture bottles, and finally, the cultured mature DC cells and the CIK cells are subjected to mixed culture, the culture bottles need to be replaced for multiple times in the middle, the process is complicated, and the cell pollution is easily caused.
Through retrieval, chinese patent No. CN207193299U discloses a culture flask for culturing DC-CIK cells, which comprises a flask body and a gas-permeable and hydrophobic flask cap, wherein the flask body comprises a horizontal portion and an inclined portion, the horizontal portion and the inclined portion are integrally formed, the flask cap is matched with the inclined portion of the flask body, a horizontal partition plate is arranged in the flask body, the horizontal portion of the flask body is divided into a flask horizontal portion I and a flask horizontal portion II, the horizontal partition plate is fixedly connected with an inclined partition plate, the inclined partition plate is inclined upwards, a gap is formed between one end of the inclined partition plate, which is close to the flask cap, and the bottom of the flask cap, and the gap forms a mixing channel for. The culture flask for culturing DC-CIK cells in the above patent has the following disadvantages: this blake bottle makes the culture solution drip at bottle horizontal part II easily when bottle horizontal part I adds the culture solution, and then influences bottle horizontal part II's cell culture, leads to the experimental failure of cell culture, reduces cell culture's success rate.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving among the prior art blake bottle and when bottle horizontal part I adds the culture solution, make the culture solution drip at bottle horizontal part II easily, and then influence bottle horizontal part II's cell culture, lead to the experimental failure of cell culture, reduce the problem of cell culture success rate, and the blake bottle for the cultivation DC-CIK cell that provides.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
A culture bottle for culturing DC-CIK cells comprises a bottle body and a breathable and hydrophobic bottle cap, wherein the bottle body comprises a horizontal part and an inclined part, the bottle cap is matched with the inclined part of the bottle body, a horizontal clapboard is fixedly connected in the bottle body, the horizontal part of the bottle body comprises a bottle body horizontal part I and a bottle body horizontal part II, the side wall of the horizontal clapboard is fixedly connected with an inclined clapboard, the inclined clapboard inclines upwards, a gap is formed between one end, close to the bottle cap, of the inclined clapboard and the bottom of the bottle cap, a mixing channel communicated with the bottle body horizontal part I and the bottle body horizontal part II is formed at the gap, the inner wall of the bottle body is fixedly connected with a connecting block, a cavity is formed in the connecting block, a moving block is movably connected to the inner wall of the cavity, a circular through hole is formed in the upper surface of the, the two ends of the first spring are fixedly connected with the inner wall of the cavity and the surface of the moving block respectively, the two ends of the flow guide pipe penetrate through the side wall of the cavity and extend outwards, an opening is formed in the side wall of the bottle body, and the inner wall of the opening is movably connected with a limiting mechanism.
Preferably, the limiting mechanism comprises a sliding block movably connected with the inner wall of the opening, a through hole is formed in the lower surface of the sliding block, a fixing rod is fixedly connected to the hole wall of the through hole, the bottom end of the fixing rod penetrates through the upper surface of the connecting block and is movably connected with the surface of the moving block, the top end of the fixing rod penetrates through the top of the opening and the side wall of the bottle body, a second spring is movably connected to the rod wall of the fixing rod, and two ends of the second spring are fixedly connected with the inner wall of the opening and the surface of the sliding block respectively.
preferably, one end of the bottle body, which is far away from the bottle cap, is fixedly connected with a convex lens.
Preferably, the side wall of the sliding block is fixedly connected with a rubber pad.
Preferably, a plurality of air holes are formed in the surface of the bottle cap, and a waterproof and breathable film is fixedly connected in the bottle cap.
Preferably, the lower surface of the bottle body is fixedly connected with a plurality of rubber gaskets.
Compared with the prior art, the utility model provides a cultivate DC-CIK blake bottle for cell possesses following beneficial effect:
1. The culture bottle for culturing DC-CIK cells is provided with a guide pipe, a first spring, a connecting block and a moving block, when the culture solution is added to the horizontal part II of the bottle body, the guide pipe is not lifted, so that the culture solution of the horizontal part II of the bottle body is prevented from adhering to the guide pipe, then the culture solution is dripped on the horizontal part I of the bottle body, when the culture solution is added on the horizontal part I of the bottle body, the fixed rod is pulled, the fixed rod drives the slide block to move and compress the second spring, the fixed rod is separated from the range of the movable block, then the movable block drives the flow guide pipe to move upwards along the cavity in the connecting block under the elastic force action of the first spring, so that the flow guide pipe reaches the bottle opening, and the culture solution on the horizontal part I of the bottle body is conveniently added, the structure can not make the culture liquid injected on the bottle body horizontal part I drop on the bottle body horizontal part II, thereby ensuring that the cell culture can be successful and improving the success rate of the cell culture.
2. This cultivate DC-CIK blake bottle for cell through being provided with convex lens and rubber gasket, through being provided with the enlarged ability of convex lens, can make things convenient for the cultivater to watch the state of the in-vivo cell culture of bottle, through the frictional force of rubber gasket increase bottle and contact surface, improves the bottle and places for stability.
the part that does not relate to in the device all is the same with prior art or can adopt prior art to realize, the utility model discloses the culture liquid that does not make filling on the bottle horizontal part I drips on bottle horizontal part II, and then has ensured that cell culture can succeed, has improved cell culture's success rate, also can improve the bottle and place for stability.
Drawings
FIG. 1 is a schematic structural diagram of a culture flask for culturing DC-CIK cells according to the present invention;
FIG. 2 is a schematic structural diagram of part A of a culture flask for culturing DC-CIK cells according to the present invention.
In the figure: 1 bottle, 2 bottle lids, 3 connecting blocks, 4 cavities, 5 moving blocks, 6 honeycomb ducts, 7 first springs, 8 openings, 9 sliders, 10 fixing rods, 11 second springs, 12 convex lenses, 13 air vents, 14 waterproof breathable films and 15 rubber gaskets.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Referring to fig. 1-2, a culture bottle for culturing DC-CIK cells, comprising a bottle body 1 and a gas-permeable and hydrophobic bottle cap 2, wherein the bottle body 1 comprises a horizontal portion and an inclined portion, the bottle cap 2 is matched with the inclined portion of the bottle body 1, a horizontal partition is fixedly connected in the bottle body 1, the horizontal portion of the bottle body 1 is composed of a bottle body horizontal portion I and a bottle body horizontal portion II, a side wall of the horizontal partition is fixedly connected with an inclined partition, the inclined partition is inclined upwards, a gap is formed between one end of the inclined partition, which is close to the bottle cap, and the bottom of the bottle cap 2, and the gap forms a mixing channel for communicating the bottle body horizontal portion I with the bottle body horizontal portion II, a connecting block 3 is fixedly connected to an inner wall of the bottle body 1, a cavity 4 is formed in the connecting block 3, a moving block 5 is movably connected to the inner wall of, the both ends of first spring 7 are connected with the fixed surface of the inner wall of cavity 4 and movable block 5 respectively, and the both ends of honeycomb duct 6 all pass the lateral wall of cavity 4 and outwards extend, and opening 8 has been seted up to 1 lateral wall of bottle, and opening 8's inner wall swing joint has stop gear.
Stop gear includes slider 9 with 8 inner wall swing joint of opening, the through-hole has been seted up to the lower surface of slider 9, and the pore wall fixedly connected with dead lever 10 of through-hole, the upper surface of connecting block 3 and the surperficial swing joint of movable block 5 are passed to the bottom of dead lever 10, the top of opening 8 and the lateral wall of bottle 1 are passed on the top of dead lever 10, the pole wall swing joint of dead lever 10 has second spring 11, the both ends of second spring 11 respectively with the inner wall of opening 8 and the fixed surface of slider 9 is connected, honeycomb duct 6's removal can be controlled to this structure.
one end fixedly connected with convex lens 12 that bottle lid 2 was kept away from to bottle 1, through being provided with the enlarged ability of convex lens 12, can make things convenient for culturist to watch the state of cell culture in the bottle 1.
The lateral wall fixedly connected with rubber pad of slider 9, the rubber pad can increase slider 9's leakproofness.
A plurality of air holes 13 are formed in the surface of the bottle cap 2, a waterproof breathable film 14 is fixedly connected in the bottle cap 2, and the air holes 13 and the waterproof breathable film 14 can ensure air circulation in the bottle body 1.
The lower fixed surface of bottle 1 is connected with a plurality of rubber gasket 15, through the frictional force of rubber gasket 15 increase bottle 1 and contact surface, improves bottle 1 and places for stability.
In the utility model, when the culture solution is added to the horizontal part II of the bottle body, the guide pipe 6 is not lifted at this time, the culture solution of the horizontal part II of the bottle body 1 is prevented from attaching to the guide pipe 6 and further dripping on the horizontal part I of the bottle body, when the culture solution is added to the horizontal part I of the bottle body, the fixed rod 10 is pulled, the fixed rod 10 drives the slider 9 to move and compress the second spring 11, the fixed rod 10 is separated from the range of the movable block 5, then the movable block 5 drives the guide pipe 6 to move upwards along the cavity 4 in the connecting block 3 under the elastic action of the first spring 7, and further the guide pipe 6 reaches the bottle mouth, thereby facilitating the culture solution filling on the horizontal part I of the bottle body, the structure can not lead the culture solution filled on the horizontal part I of the bottle body to drip on the horizontal part II of the bottle body, thereby ensuring the success of cell culture, improving the, can conveniently cultivate the state that the person watched cell culture in the bottle 1, through the frictional force of 15 increase bottles of rubber gasket 1 and contact surface, improve bottle 1 and place for stability.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (6)
1. A culture bottle for culturing DC-CIK cells comprises a bottle body (1) and a breathable and hydrophobic bottle cap (2), wherein the bottle body (1) comprises a horizontal part and an inclined part, the bottle cap (2) is matched with the inclined part of the bottle body (1), a horizontal clapboard is fixedly connected in the bottle body (1), the horizontal part of the bottle body (1) comprises a bottle body horizontal part I and a bottle body horizontal part II, the side wall of the horizontal clapboard is fixedly connected with an inclined clapboard, the inclined clapboard inclines upwards, a gap is formed between one end, close to the bottle cap, of the inclined clapboard and the bottom of the bottle cap (2), and the gap forms a mixing channel communicated with the bottle body horizontal part I and the bottle body horizontal part II, and is characterized in that the inner wall of the bottle body (1) is fixedly connected with a connecting block (3), a cavity (4) is formed in the connecting block (3), and a moving block (5, circular through-hole has been seted up to the upper surface of movable block (5), and the pore wall fixedly connected with honeycomb duct (6) of circular through-hole, first spring (7) have been cup jointed in the outer wall activity of honeycomb duct (6), the both ends of first spring (7) are connected with the fixed surface of the inner wall of cavity (4) and movable block (5) respectively, the both ends of honeycomb duct (6) all pass the lateral wall of cavity (4) and outwards extend, opening (8) have been seted up to bottle (1) lateral wall, the inner wall swing joint of opening (8) has stop gear.
2. The culture bottle for culturing DC-CIK cells as claimed in claim 1, wherein the limiting mechanism comprises a sliding block (9) movably connected with the inner wall of the opening (8), the lower surface of the sliding block (9) is provided with a through hole, the wall of the through hole is fixedly connected with a fixing rod (10), the bottom end of the fixing rod (10) passes through the upper surface of the connecting block (3) and is movably connected with the surface of the moving block (5), the top end of the fixing rod (10) passes through the top of the opening (8) and the side wall of the bottle body (1), the wall of the fixing rod (10) is movably connected with a second spring (11), and the two ends of the second spring (11) are respectively fixedly connected with the inner wall of the opening (8) and the surface of the sliding block (9).
3. The culture flask for culturing DC-CIK cells, according to claim 1, wherein the end of the flask body (1) far away from the flask cover (2) is fixedly connected with a convex lens (12).
4. The culture flask for culturing DC-CIK cells, according to claim 2, wherein the side wall of the slide block (9) is fixedly connected with a rubber pad.
5. The culture bottle for culturing DC-CIK cells as claimed in claim 1, wherein the surface of the bottle cap (2) is provided with a plurality of air holes (13), and a waterproof and breathable film (14) is fixedly connected in the bottle cap (2).
6. The culture flask for culturing DC-CIK cells, according to claim 1, wherein a plurality of rubber gaskets (15) are fixedly connected to the lower surface of the flask body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822107053.XU CN209797987U (en) | 2018-12-16 | 2018-12-16 | Culture bottle for culturing DC-CIK cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822107053.XU CN209797987U (en) | 2018-12-16 | 2018-12-16 | Culture bottle for culturing DC-CIK cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209797987U true CN209797987U (en) | 2019-12-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822107053.XU Active CN209797987U (en) | 2018-12-16 | 2018-12-16 | Culture bottle for culturing DC-CIK cells |
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| Country | Link |
|---|---|
| CN (1) | CN209797987U (en) |
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2018
- 2018-12-16 CN CN201822107053.XU patent/CN209797987U/en active Active
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