CN217025975U - Disposable NK cell culture bottle convenient to transfer - Google Patents
Disposable NK cell culture bottle convenient to transfer Download PDFInfo
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- CN217025975U CN217025975U CN202123292975.0U CN202123292975U CN217025975U CN 217025975 U CN217025975 U CN 217025975U CN 202123292975 U CN202123292975 U CN 202123292975U CN 217025975 U CN217025975 U CN 217025975U
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- culture bottle
- bottle body
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- culture
- tube
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Abstract
The utility model discloses a disposable NK cell culture bottle convenient to transfer, which comprises a culture bottle body, wherein a culture dish is arranged at the bottom in the culture bottle body, a threaded cover is in threaded connection with the top of the culture bottle body, an extraction tube is arranged on the threaded cover, a telescopic cylinder is arranged at the top of the extraction tube, the inner wall of the telescopic cylinder is connected with a limiting disc through a telescopic spring, the bottom of the limiting disc is connected with a telescopic piston through a connecting column, and a detachable discharge tube is in threaded connection with one side of the extraction tube. According to the utility model, the culture dish is arranged at the bottom of the culture bottle for culturing cells, during extraction, the culture dish is moved to be in contact with the extraction tube arranged on the threaded cover, the air pressure sealing state of the culture bottle body is changed through the balance tube, the cells in the culture dish are continuously extracted through the telescopic piston, and are discharged through the detachable discharge tube, so that the cells in the culture bottle body can be taken out at one time, and the cells are not polluted.
Description
Technical Field
The utility model relates to the technical field of culture bottles, in particular to a disposable NK cell culture bottle convenient to transfer.
Background
In vitro culture of cells and tissues has become an essential element in life research and practice. The cell types in culture are numerous, from viruses to bacteria and fungi, from human cells to animal and plant cells. Some cells and tissues can grow in suspension, but a significant proportion of mammalian cells require surface adherence. Therefore, in addition to good transparency and non-toxic and sterile, culture flasks, plates and dishes used to provide an environment for in vitro cell culture need to be surface modified to allow for attachment, division and growth.
Present blake bottle utilizes the straw to extract when drawing, and the volume of drawing at every turn receives the restriction, can't shift disposable blake bottle's cell whole simultaneously to in the straw draws the in-process, need the bottle lid of opening many times, opening of bottle lid can make the pollutant very easily enter into the blake bottle in, cause the pollution to the cell, the experiment of sample after the influence shifts, for this now proposes a disposable NK cell blake bottle convenient to shift.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the problems in the prior art and provides a disposable NK cell culture bottle convenient to transfer.
In order to achieve the purpose, the utility model adopts the following technical scheme:
the utility model provides a disposable NK cell culture bottle convenient to shift, includes the blake bottle body, the bottom is provided with the culture dish in the blake bottle body, blake bottle body top threaded connection has the screw cap, be provided with the extraction tube on the screw cap, the extraction tube top is provided with telescopic cylinder, telescopic cylinder inner wall is connected with spacing dish through expanding spring, spacing dish upper portion is provided with the control, spacing portion of trying to get to the heart of a matter is connected with telescopic piston through the spliced pole, extraction tube one side threaded connection has the detachable discharge pipe.
Preferably, its bottom of culture dish is connected with the support column through the connection pad, the support column runs through culture bottle body downwardly extending to fixedly connected with saucer, the saucer is connected with culture bottle body through the supporting spring of cover on the support column lateral wall.
Preferably, the control piece includes the control post with spacing dish fixed connection, the telescopic cylinder is run through to the control post top and outwards extends to fixedly connected with control ball.
Preferably, a balance pipe for balancing air pressure is arranged on the threaded cover, and a sealing plug is arranged on the balance pipe.
Preferably, the side wall of the extraction pipe is provided with a threaded connector, and the detachable discharge pipe is in threaded connection with the threaded connector.
Preferably, an inclined clinging ring is arranged at the top of the culture dish and connected with the inner wall of the culture bottle body.
Compared with the prior art, the utility model has the beneficial effects that:
the utilization sets up the culture dish bottom the blake bottle and cultivates the cell, when drawing, removes the blake bottle and contacts with the extraction tube that sets up on the thread cover, changes the atmospheric pressure seal state of blake bottle body through the balance pipe to utilize telescopic piston to realize constantly drawing to the cell that is located the blake bottle, realize discharging through can dismantling the discharge tube, so relapse, can realize once only taking out the cell in the blake bottle body, and can not cause the pollution to the cell.
Drawings
FIG. 1 is a schematic diagram of the main structure of a disposable NK cell culture bottle convenient for transfer according to the present invention;
FIG. 2 is a schematic view of the top view of a disposable NK cell culture bottle convenient for transfer according to the present invention;
FIG. 3 is a schematic cross-sectional view illustrating a disposable NK cell culture bottle for convenient transfer according to the present invention.
In the figure: the culture flask comprises a culture flask body 1, a culture dish 2, a threaded cover 3, an extraction tube 4, a telescopic cylinder 5, a limiting disc 6, a telescopic piston 7, a detachable discharge tube 8, a support column 9, a cushion disc 10, a control column 11, a control ball 12, a balance tube 13 and an oblique clinging ring 14.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
Referring to fig. 1-3, a disposable NK cell culture bottle convenient for transferring comprises a culture bottle body 1, a culture dish 2 is arranged at the bottom in the culture bottle body 1, an oblique clinging ring 14 is arranged at the top of the culture dish 2, the oblique clinging ring 14 is connected with the inner wall of the culture bottle body 1, and the cells are prevented from generating pollution to the culture bottle body 1 in the culture process; its bottom of culture dish 2 is connected with support column 9 through the connection pad, and support column 9 runs through 1 downwardly extending of culture bottle body to fixedly connected with saucer 10, saucer 10 is connected with culture bottle body 1 through the supporting spring that the cover was established on support column 9 lateral wall, wherein can make the culture dish 2 that saucer 10 carries out the connection through support column 9 shift up through pressing culture bottle body 1, thereby be convenient for the absorption of extraction tube 4 to the cell.
1 top threaded connection of culture bottle body has screw cap 3, is provided with the balanced pipe 13 of balanced atmospheric pressure on the screw cap 3, is provided with the sealing plug on the balanced pipe 13, utilizes to set up balanced pipe 13 for when stopping up balanced pipe 13, press telescopic piston 7, can't make in the liquid of extraction tube 4 enters into culture bottle body 1, thereby utilize atmospheric pressure to realize outwards discharging the cell.
Be provided with extraction tube 4 on the screw cap 3, 4 tops of extraction tube are provided with telescopic tube 5, 5 inner walls of telescopic tube have spacing dish 6 through expanding spring connection, wherein telescopic spring applys for spacing dish 6 decurrent pulling force, make telescopic piston 7 when the atress not, be in extraction tube 4 bottom department, ensure that culture bottle body 1 is in encapsulated situation, 6 upper portions of spacing dish are provided with the control, the control includes the control post 11 with 6 fixed connection of spacing dish, the outside extension of telescopic tube 5 is run through at 11 tops of control post, and fixedly connected with control ball 12, be convenient for carry out manual pulling.
Spacing 6 bottoms of dish are connected with telescopic piston 7 through the spliced pole, and 4 one sides threaded connection of extraction tube have can dismantle discharge pipe 8, and the threaded connection mouth has been seted up to 4 lateral walls of extraction tube, can dismantle discharge pipe 8 and threaded connection mouth threaded connection, and the realization can be dismantled, can change as required.
Specifically, when the culture dish is used, cells are cultured in the culture dish 2, the culture dish 2 is positioned in the culture bottle body 1 to form a culture space, when the cells are taken out during culture, the culture bottle body 1 is pressed downwards, so that the distance between the pad disc 10 and the culture bottle body 1 is reduced, the pad disc 10 positioned at the bottom of the culture bottle body 1 is moved upwards, the culture dish 2 is contacted with the extraction tube 4, the sealing plug on the balance tube 13 on one side is pulled out, the limit disc 6 drives the telescopic piston 7 to move upwards through the extraction control column 11, cells in the culture dish 2 are sucked, and the sucked cells are positioned in the extraction tube 4;
close balanced pipe 13 through the sealing plug for the culture bottle body 1 of bottom is in encapsulated situation, makes the cell that is in the extraction tube 4 through promoting telescopic piston 7 this moment and realizes discharging through can dismantling discharge pipe 8, thereby realizes the extraction to the cell, so relapse, can realize once only taking out the cell in the culture bottle body 1, and can not cause the pollution to the cell.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent alternatives or modifications according to the technical solution of the present invention and the inventive concept thereof should be covered by the scope of the present invention.
Claims (6)
1. The utility model provides a disposable NK cell culture bottle convenient to shift, includes culture bottle body (1), its characterized in that, the bottom is provided with culture dish (2) in culture bottle body (1), culture bottle body (1) top threaded connection has screw cap (3), be provided with extraction tube (4) on screw cap (3), extraction tube (4) top is provided with telescopic cylinder (5), telescopic cylinder (5) inner wall is connected with spacing dish (6) through expanding spring, spacing dish (6) upper portion is provided with the control, spacing dish (6) bottom is connected with telescopic piston (7) through the spliced pole, extraction tube (4) one side threaded connection has detachable discharge pipe (8).
2. The disposable NK cell culture bottle convenient for transfer according to claim 1, wherein the bottom of the culture dish (2) is connected with a support column (9) through a connection plate, the support column (9) extends downwards through the culture bottle body (1) and is fixedly connected with a pad disc (10), and the pad disc (10) is connected with the culture bottle body (1) through a support spring sleeved on the outer side wall of the support column (9).
3. The disposable NK cell culture bottle convenient for transfer according to claim 1, wherein the control member comprises a control column (11) fixedly connected with a limiting disc (6), the top of the control column (11) extends outwards through the telescopic cylinder (5) and is fixedly connected with a control ball (12).
4. The disposable NK cell culture bottle for facilitating transfer according to claim 1, wherein a balance tube (13) for balancing air pressure is provided on the screw cap (3), and a sealing plug is provided on the balance tube (13).
5. The disposable NK cell culture bottle convenient for transferring according to claim 1, wherein the side wall of the extraction tube (4) is provided with a threaded connector, and the detachable discharge tube (8) is in threaded connection with the threaded connector.
6. The disposable NK cell culture bottle convenient for transfer according to claim 1, wherein the top of the culture dish (2) is provided with an inclined clinging ring (14), and the inclined clinging ring (14) is connected with the inner wall of the culture bottle body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123292975.0U CN217025975U (en) | 2021-12-25 | 2021-12-25 | Disposable NK cell culture bottle convenient to transfer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123292975.0U CN217025975U (en) | 2021-12-25 | 2021-12-25 | Disposable NK cell culture bottle convenient to transfer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217025975U true CN217025975U (en) | 2022-07-22 |
Family
ID=82442923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123292975.0U Active CN217025975U (en) | 2021-12-25 | 2021-12-25 | Disposable NK cell culture bottle convenient to transfer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217025975U (en) |
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2021
- 2021-12-25 CN CN202123292975.0U patent/CN217025975U/en active Active
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