CN216236993U - A microbial culture dish for bioengineering experiments - Google Patents

Abstract

The utility model discloses a microbial culture dish for bioengineering experiments, which comprises a culture box with an upward opening, a culture tank arranged in the middle of the lower end face of an inner cavity of the culture box, a pressure plate arranged in the culture tank, a support assembly arranged between the pressure plate and the culture tank, a sealing cover plate sleeved with the culture box, and a handle arranged in the middle of the upper end face of the sealing cover plate. According to the microbial culture dish for bioengineering experiments, the pressing plate is arranged in the culture tank, so that the cover glass can be fixed conveniently through the pressing plate, the adherent growth environment of cells is effectively ensured, and the cell detachment is avoided.

Description

A microbial culture dish for bioengineering experiments

technical field

The utility model relates to the related technical field of petri dishes, in particular to a microorganism petri dish for bioengineering experiments.

Background technique

A petri dish is a laboratory vessel used for microbial or cell culture. It consists of a flat disc-shaped bottom and a lid. It is generally made of glass or plastic. The materials of petri dishes are basically divided into two categories, mainly plastic. And glass, glass can be used for plant material, microbial culture and animal cell adherent culture may also be used, plastic may be polyethylene material, disposable and multiple use, suitable for laboratory inoculation, The operation of streaking and separating bacteria can be used for the cultivation of plant materials;

In the petri dish in the prior art, a hole is usually made at the bottom of the petri dish, and then a cover glass or cell climbing sheet with a thickness of 170-220 μm is adhered to the bottom of the petri dish with shadowless glue or silica gel, which is suitable for the observation area. Only in the perforated area, and due to the hydrophobicity of the glass of the petri dish, when culturing cells, the cells cannot adhere well to the glass, and the growth state is not good, even when doing confocal scanning imaging. The phenomenon of cell dissociation occurs, which cannot meet the current needs of various use of petri dishes;

To this end, we propose a microbial petri dish for bioengineering experiments.

Utility model content

The purpose of the present invention is to provide a microbial culture dish for bioengineering experiments, which aims to improve the problems raised in the above background technology.

In order to achieve the above purpose, the present invention is achieved through the following technical solutions: a microbial culture dish for bioengineering experiments, comprising a culture box with an upward opening, a culture tank arranged in the middle of the lower end face of the inner cavity of the culture box, a A pressure plate in the culture tank, a support assembly arranged between the pressure plate and the culture tank, a sealing cover plate sleeved with the culture box, and a sealing cover plate disposed in the middle of the end face of the sealing cover plate handle.

As a preferred aspect of the present invention, the culture box is made of plastic material, and the inner wall of the culture tank is set as a smooth arc surface.

By adopting the above technical solution, it is further convenient to culture the cells through the culture tank.

As a preferred aspect of the present invention, a first limit baffle is arranged on the upper middle and upper part of the outer periphery of the culture box, and the first limit baffle is circumferentially arranged with the center of the culture box as the center point. The limit baffle is fixedly connected with the culture box.

By adopting the above technical solution, it is convenient to support the lower end of the sealing cover plate by the first limiting baffle plate, so as to facilitate the connection of the sealing cover plate and the culture box.

As a preferred aspect of the present invention, support feet are provided on the periphery of the lower end of the culture box, and the support feet are fixedly connected with the culture box.

By adopting the above technical solution, it is convenient to support the culture box by the support feet, and it is convenient for the staff to observe the cells in the culture box.

As a preferred aspect of the present invention, the support assembly includes a second limit baffle fixedly arranged on the outer periphery of the upper end of the culture tank and a connecting wing plate arranged on the outer circumference of the pressing plate, the connecting wing plate is fixed to the pressing plate connected, and the outer diameter of the connecting wing plate is larger than the outer diameter of the second limiting baffle.

By adopting the above technical solution, it is convenient to support the connecting wing plate by the second limit baffle plate, so as to facilitate the pressing plate to fix the glass slide in the culture tank.

Compared with the prior art, the beneficial effects of the present utility model are as follows: the utility model has the characteristics of reasonable design and simple operation, and the microorganism culture dish for bioengineering experiments of the present utility model is provided with a pressure plate in the culture tank, which is convenient for The cover glass is fixed by pressing the plate, so as to effectively ensure the adherent growth environment of cells and avoid cell detachment.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the accompanying drawings required in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention. Therefore, it should not be regarded as a limitation of the scope. For those of ordinary skill in the art, other related drawings can also be obtained from these drawings without any creative effort.

Fig. 1 is the structural representation of the overall structure of the microorganism culture dish for bioengineering experiments of the present utility model (when the sealing cover plate is half-cut);

Fig. 2 is the structural representation of the partial explosion diagram in the microorganism culture dish for bioengineering experiments of the present utility model;

3 is a schematic structural diagram of a half cross-sectional view of the overall structure of the microbial culture dish for bioengineering experiments of the present invention;

FIG. 4 is a schematic structural diagram of the enlarged view of the position A of FIG. 3 in the microorganism culture dish for bioengineering experiments of the present invention.

In the figure: 1-Cultivation box, 11-Support feet, 12-First limit baffle, 13-Cultivation tank, 131-Second limit baffle, 2-Press plate, 21-Connecting wing plate, 3-Sealing cover plate, 31-handle.

Detailed ways

In order to make the purposes, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described The embodiments of the present invention are a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention. Accordingly, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

Please refer to Figure 1, Figure 2, Figure 3 and Figure 4, the present utility model provides a technical solution: a microbial culture dish for bioengineering experiments, comprising a culture box 1 with an opening facing upward, arranged under the inner cavity of the culture box 1 The culture tank 13 at the middle position of the end face, the pressure plate 2 arranged in the culture tank 13, the support assembly arranged between the pressure plate 2 and the culture tank 13, the sealing cover plate 3 sleeved with the culture box 1, and the sealing cover plate 3. The handle 31 at the middle of the upper end face, in the present invention, because the pressure plate 2 is arranged in the culture tank 13, it is convenient to fix the cover glass by the pressure plate 2, thereby effectively ensuring the adherent growth of cells. environment to avoid cell dissociation.

Please refer to FIGS. 1 , 2 , 3 and 4 , the culture box 1 is made of plastic material, and the inner wall of the culture tank 13 is set as a smooth arc surface, so that the cells can be cultured easily through the culture tank 13 .

Please refer to FIGS. 1 , 2 , 3 and 4 , in order to facilitate the connection of the sealing cover 3 to the culture box 1 , a first limit baffle 12 is provided on the upper middle and upper part of the outer periphery of the culture box 1 . The plate 12 is circumferentially arranged with the center of the culture box 1 as the center point, and the first limit baffle 12 is fixedly connected with the culture box 1, so as to facilitate the support of the lower end of the sealing cover plate 3 through the first limit baffle 12, thereby It is convenient to connect the sealing cover 3 to the culture box 1 .

Please refer to Figure 1, Figure 2, Figure 3 and Figure 4, in order to avoid the direct contact between the culture box 1 and the contact surface, and it is inconvenient for the staff to take the culture box 1, the outer periphery of the lower end of the culture box 1 is provided with support feet 11, supporting The feet 11 are fixedly connected with the culture box 1 , thereby facilitating the support of the culture box 1 by the support feet 11 , thereby facilitating the staff to observe the cells in the culture box 1 .

Please refer to FIGS. 1 , 2 , 3 and 4 , the support assembly includes a second limiting baffle 131 fixedly arranged on the outer periphery of the upper end of the culture tank 13 and a connecting wing plate 21 arranged on the outer periphery of the pressing plate 2 . The connecting wing plate 21 and The pressure plate 2 is fixedly connected, and the outer diameter of the connecting wing plate 21 is larger than the outer diameter of the second limit baffle 131 , thereby facilitating the support of the connecting wing plate 21 by the second limit baffle 131 , thereby facilitating the pressure plate 2 on the culture tank. The glass slide in 13 is fixed, and because the outer diameter of the connecting wing plate 21 is larger than the outer diameter of the second limiting baffle 131 , it is convenient for the staff to take the pressing plate 2 .

Working principle: When using, first place the glass slide with cells in the culture tank 13, then place the pressure plate 2 in the culture tank 13, until the connecting wing plate 21 on the periphery of the pressure plate 2 and the No. The two limiting baffles 131 are in contact, and then the sealing cover 3 is sleeved with the culture box 1 until the lower end of the sealing cover 3 is in contact with the second limiting baffle 131 .

The device obtained by the above design can basically meet the requirements of the culture dish in the prior art, and the area suitable for observation is only the area where the hole is drilled. In addition, due to the hydrophobicity of the glass of the culture dish, the cells cannot adhere well to the glass. , the growth state is not good, and even in the case of confocal scanning imaging, the phenomenon of cell dissection occurs, but in order to further improve its function, the designer has further improved the device.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims (6)

1. A microbial culture dish for bioengineering experiments, characterized in that it comprises a culture box (1) with an opening facing upward, a culture tank (13) arranged in the middle of the lower end face of the inner cavity of the culture box (1), a culture tank (13) provided The pressure plate (2) in the culture tank (13), the support assembly arranged between the pressure plate (2) and the culture tank (13), and the seal sleeved with the culture box (1) A cover plate (3) and a handle (31) arranged in the middle of the upper end face of the sealing cover plate (3). 2 . The microbial culture dish for bioengineering experiments according to claim 1 , wherein the culture box ( 1 ) is made of plastic material, and the inner wall of the culture tank ( 13 ) is set as a smooth arc surface. 3 . . 3. A microbial culture dish for bioengineering experiments according to claim 2, characterized in that, a first limit baffle plate (12) is provided in the middle and upper part of the outer periphery of the culture box (1), and the first limit baffle plate (12) The limit baffle (12) is circumferentially arranged with the center of the culture box (1) as the center point, and the first limit baffle (12) is fixedly connected with the culture box (1). 4. A microbial culture dish for bioengineering experiments according to claim 3, characterized in that a support foot (11) is provided on the periphery of the lower end of the culture box (1), and the support foot (11) is connected to the culture box (1). Box (1) is fixedly connected. 5. A microbial culture dish for bioengineering experiments according to claim 4, characterized in that the support assembly comprises a second limit baffle plate (131) fixedly arranged on the outer periphery of the upper end of the culture tank (13). and a connecting wing plate (21) arranged on the outer periphery of the pressing plate (2). The microbial culture dish for bioengineering experiments according to claim 5, characterized in that the connecting wing plate (21) is fixedly connected to the pressing plate (2), and the outer diameter of the connecting wing plate (21) is greater than The outer diameter of the second limiting baffle (131).

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