CN218710558U

CN218710558U - Novel cell culture orifice plate

Info

Publication number: CN218710558U
Application number: CN202223045129.3U
Authority: CN
Inventors: 陈昊宇; 王天倚
Original assignee: Qingdao Agricultural University
Current assignee: Qingdao Agricultural University
Priority date: 2022-11-16
Filing date: 2022-11-16
Publication date: 2023-03-24
Anticipated expiration: 2032-11-16

Abstract

The utility model relates to a cell culture field especially relates to a novel cell culture orifice plate. Novel cell culture orifice plate is including cultivateing board, shutoff subassembly and sealing component, be equipped with the top cap on the cultivation board, and be equipped with the handle hole that corresponds quantity with the culture tank on the top cap, all install threaded connection's sealing plug in the handle hole. The utility model provides a novel cell culture orifice plate, when extracting the cell sap in the culture tank, the sealing plug that corresponds is unscrewed to usable knob, the culture tank that the cell drawed need to carry out this moment can expose, use the pipette to extract the cell sap in the culture tank through the handle hole, later screw the sealing plug into the handle hole once more, carry out the shutoff to the handle hole can, avoid because of when extracting the cell sap in the culture tank, need open whole top cap, lead to destroying the problem of other culture tank inner cell sap easily.

Description

Novel cell culture orifice plate

Technical Field

The utility model relates to a cell culture field especially relates to a novel cell culture orifice plate.

Background

The cell culture pore plate is a biological tool for culturing cells, is common in the biological field and has more categories, the most common is a six-pore flat cell culture plate, and cell liquid is put into a pore groove on the culture plate and sealed;

the cell culture pore plate is widely applied to the field of biology due to the advantages of convenience in use, low manufacturing cost, good transparency, no toxicity and sterility, and when the cell culture pore plate is used and multiple groups of cells are cultured simultaneously, the same culture environment of each group of cells can be ensured, so that the cell culture pore plate is an indispensable tool in the field of biology.

When the existing cell culture pore plate is used, when cells in the existing cell culture pore plate are extracted, the whole top cover needs to be opened, and at the moment, cell sap in other pore grooves is also exposed in the air, so that the cell sap in the pore grooves is easily damaged.

Therefore, there is a need to provide a new cell culture well plate to solve the above technical problems.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides a novel cell culture pore plate.

The utility model provides a novel cell culture orifice plate includes:

the culture plate is provided with a plurality of culture grooves on two sides of the top of the culture plate, and communication grooves are formed between the culture grooves on two sides of the top of the culture plate;

a plugging component, which is positioned in the culture plate;

the sealing assembly is located on the culture plate and comprises a top cover, an operation hole and a sealing plug, the top cover is arranged on the culture plate, the operation hole corresponding to the culture tank in quantity is formed in the top cover, and the sealing plug connected with threads is installed in the operation hole.

Preferably, all install fixed connection's spacing ring on the inner wall of culture tank, and the bottom in intercommunication groove all is equipped with the spacing groove.

Preferably, the plugging assembly comprises a sealing block, a clamping block and an anti-slip groove, the sealing block is connected in a sliding mode and is arranged in the communicating groove, the clamping block is fixedly connected to the bottom of the sealing block, the clamping block is connected with the limiting groove in a sliding mode, and the anti-slip groove is formed in each of two sides of the sealing block.

Preferably, the sealing assembly further comprises a knob and a buckle, the knob is fixedly connected to the top of the sealing plug, and the buckle is rotatably connected to two sides of the top cover.

Preferably, the bottom plate of fixed connection is installed to the bottom of culture plate, and the limiting plate that uses with the buckle cooperation is all installed to the both sides of bottom plate.

Preferably, the other two sides of the bottom plate are provided with finger grooves, and four corners of the bottom plate are provided with support legs which are fixedly connected.

Preferably, the sealing plug is provided with a rubber sealing gasket, and the knob is provided with anti-skidding teeth.

Compared with the prior art, the utility model provides a novel cell culture orifice plate has following beneficial effect:

1. the utility model discloses when extracting the cell sap in the culture tank, usable knob unscrews the sealing plug that corresponds, the culture tank that needs to carry out the cell extraction this moment can expose, use the pipette to draw out the cell sap in the culture tank through the handle hole, later screw in the handle hole with the sealing plug once more, carry out the shutoff to the handle hole can, avoid because of when drawing the cell sap in the culture tank, need open whole top cap, lead to destroying the problem of other culture tank cell sap easily.

2. The utility model discloses when cultivateing the cell to the culture tank in, if need mix the cell sap of two culture tanks and when cultivateing, can take sealed piece out from the intercommunication inslot, connect through the intercommunication groove between two culture tanks this moment to can make the cell sap in two culture tanks mix through the intercommunication groove, improve the device and to the complementary of cell culture work.

Drawings

FIG. 1 is a schematic structural view of a preferred embodiment of the novel cell culture well plate provided by the present invention;

FIG. 2 is a schematic diagram of the structure of the plate and the blocking assembly shown in FIG. 1;

FIG. 3 is a schematic view of the inside structure of the plate shown in FIG. 2;

FIG. 4 is a schematic structural view of the base plate shown in FIG. 1;

FIG. 5 is a schematic structural view of the plugging assembly of FIG. 2;

fig. 6 is a schematic structural view of the sealing assembly shown in fig. 1.

Reference numbers in the figures: 1. culturing a plate; 11. a culture tank; 12. a communicating groove; 13. a limiting ring; 14. a limiting groove; 2. a plugging component; 21. a sealing block; 22. a clamping block; 23. an anti-slip groove; 3. a base plate; 31. a limiting plate; 32. a finger groove; 33. a support leg; 4. a seal assembly; 41. a top cover; 42. an operation hole; 43. a sealing plug; 44. a knob; 45. and (5) buckling.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the embodiments of the present invention, it should be noted that, if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship which is usually placed when the product of the present invention is used, the description is only for convenience of description of the present invention and simplification, but the indication or suggestion that the device or element to be referred must have a specific position, be constructed and operated in a specific position, and therefore, the present invention should not be construed as being limited thereto. Furthermore, the appearances of the terms "first," "second," "third," and the like, if any, are only used to distinguish one description from another, and are not to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not require that the components be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the embodiments of the present invention, it should be further noted that unless explicitly stated or limited otherwise, the terms "disposed," "mounted," "connected," and "connected" should be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.

The following detailed description is provided to illustrate specific embodiments of the present invention.

Referring to fig. 1 to 6, a novel cell culture well plate according to an embodiment of the present invention includes: a culture plate 1, a plugging component 2 and a sealing component 4.

In the embodiment of the present invention, please refer to fig. 2 and fig. 3, a plurality of culture tanks 11 are disposed on both sides of the top of the culture plate 1, and a communication tank 12 is disposed between the culture tanks 11 on both sides of the top of the culture plate 1, a fixed connection limiting ring 13 is disposed on the inner wall of the culture tank 11, and a limiting groove 14 is disposed at the bottom of the communication tank 12.

It should be noted that: the culture plate 1 is supported by special materials, has the characteristics of no toxicity, no bacteria and the like, and when cells are cultured, cell sap is put into the culture tank 11, the surface of the cell sap needs to be noticed not to exceed the limiting ring 13, so that the phenomenon that the culture period is long due to too much cell sap is avoided, when two groups of cell sap are required to be mixed, the two culture tanks 11 can be communicated through the communicating tank 12, the cell sap in the two culture tanks 11 are mixed, and then the culture result is recorded.

In the embodiment of the present invention, please refer to fig. 2 and 5, the plugging component 2 is located the culture plate 1, the plugging component 2 includes a sealing block 21, a clamping block 22 and an anti-slip groove 23, the sealing block 21 with sliding connection is installed in the communicating groove 12, the clamping block 22 with fixed connection is installed at the bottom of the sealing block 21, the clamping block 22 is connected with the limiting groove 14 in a sliding manner, and the anti-slip groove 23 is installed at both sides of the sealing block 21.

It should be noted that: in ordinary use, the sealing block 21 is engaged in the communicating groove 12, and the engaging block 22 is engaged in the stopper groove 14, so that the two culture tanks 11 can be separated by the sealing block 21, and separate cell culture can be performed, and when cells are cultured in the culture tank 11, if it is necessary to mix and culture cell liquids in the two culture tanks 11, the sealing block 21 can be drawn out from the communicating groove 12, and at this time, the two culture tanks 11 are connected to each other through the communicating groove 12, and the cell liquids in the two culture tanks 11 can be mixed through the communicating groove 12.

In the embodiment of the present invention, please refer to fig. 3 and 4, the bottom plate 3 of the fixed connection is installed at the bottom of the cultivation plate 1, the limiting plates 31 used in cooperation with the fasteners 45 are installed on both sides of the bottom plate 3, the finger grooves 32 are installed on the other two sides of the bottom plate 3, and the supporting legs 33 of the fixed connection are installed at four corners of the bottom plate 3.

It should be noted that: the bearing capacity of bottom plate 3 multiplicable culture plate 1 bottom makes its non-deformable, can be more convenient take up bottom plate 3 and culture plate 1 through indicating groove 32, can place more stably with bottom plate 3 through landing leg 33.

In the embodiment of the utility model, please refer to fig. 1 and 6, sealing component 4 is located culture plate 1, and sealing component 4 includes top cap 41, handle hole 42 and sealing plug 43, be equipped with top cap 41 on culture plate 1, and be equipped with the handle hole 42 with culture tank 11 correspondence quantity on the top cap 41, all install threaded connection's sealing plug 43 in the handle hole 42, sealing component 4 is still including knob 44 and buckle 45, fixed connection's knob 44 is all installed at the top of sealing plug 43, and the buckle 45 of rotating the connection is all installed to the both sides of top cap 41, be equipped with rubber packing pad on the sealing plug 43, and all be equipped with anti-skidding tooth on the knob 44.

It should be noted that: under the normal use condition, the remaining sealing plug 43 cooperation of top cap 41, can seal up all culture tanks 11, provide sterile environment for the cell sap in culture tank 11, when extracting the cell sap in culture tank 11, usable knob 44 unscrews corresponding sealing plug 43, the culture tank 11 that needs to carry out the cell extraction this moment can expose, use the pipette to draw out the cell sap in culture tank 11 through handle hole 42, later screw in handle hole 42 again with sealing plug 43, it can to carry out the shutoff to handle hole 42, after top cap 41 was put on culture plate 1, rotate buckle 45, utilize buckle 45 to block limiting plate 31, can accomplish the fixed operation of top cap 41.

The utility model provides a novel cell culture orifice plate's theory of operation as follows:

firstly, ensuring that the device is not broken, then placing the device on a special clean bench, ensuring the stability of the placement of the device, then taking the top cover 41 off the culture plate 1, filling a certain amount of cell sap into the culture tank 11, limiting the surface of the cell sap to a certain degree by the limiting ring 13, preventing the excessive filling amount of the cell sap, placing the top cover 41 on the culture plate 1 after placing, rotating the buckle 45, clamping the limiting plate 31 by the buckle 45, fixing the top cover 41 on the culture plate 1, and matching the top cover 41 with the sealing plug 43 to seal the culture tank 11;

when cell sap in the culture tank 11 is extracted, the corresponding sealing plug 43 can be unscrewed by using the knob 44, the culture tank 11 which needs cell extraction can be exposed, the cell sap in the culture tank 11 can be extracted by using the pipette through the operation hole 42, and then the sealing plug 43 is screwed into the operation hole 42 again to plug the operation hole 42;

meanwhile, when the cell culture is performed in the culture tank 11, if it is necessary to mix the cell fluids of the two culture tanks 11 and perform the culture, the sealing block 21 may be drawn out from the communicating tank 12, the two culture tanks 11 may be connected to each other by the communicating tank 12, and the cell fluids in the two culture tanks 11 may be mixed by the communicating tank 12;

then, the state of the cell fluid may be periodically recorded during the cell culture.

The utility model discloses circuit and control that relate to are prior art, do not carry out too much repetition here.

The above only is the embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structures or equivalent processes of the present invention are used in the specification and the attached drawings, or directly or indirectly applied to other related technical fields, and the same principle is included in the protection scope of the present invention.

Claims

1. A novel cell culture well plate, comprising:

the culture plate (1), both sides of the top of the culture plate (1) are provided with a plurality of culture grooves (11), and communication grooves (12) are arranged between the culture grooves (11) on both sides of the top of the culture plate (1);

the plugging component (2), the plugging component (2) is positioned in the culture plate (1);

seal assembly (4), seal assembly (4) are located and cultivate board (1), and seal assembly (4) include top cap (41), handle hole (42) and sealing plug (43), be equipped with top cap (41) on cultivate board (1), and be equipped with on top cap (41) and cultivate groove (11) and correspond handle hole (42) of quantity, all install threaded connection's sealing plug (43) in handle hole (42).

2. A novel cell culture well plate according to claim 1, characterized in that the inner walls of the culture grooves (11) are all provided with a fixed connected limiting ring (13), and the bottoms of the communication grooves (12) are all provided with a limiting groove (14).

3. The novel cell culture well plate according to claim 2, wherein the plugging assembly (2) comprises sealing blocks (21), clamping blocks (22) and anti-slip grooves (23), the sealing blocks (21) are slidably connected and are mounted in the communication grooves (12), the clamping blocks (22) are fixedly connected and are mounted at the bottoms of the sealing blocks (21), the clamping blocks (22) are slidably connected with the limiting grooves (14), and the anti-slip grooves (23) are arranged on two sides of the sealing blocks (21).

4. The novel cell culture well plate according to claim 1, characterized in that the sealing assembly (4) further comprises a knob (44) and a buckle (45), the top of the sealing plug (43) is provided with the knob (44) which is fixedly connected, and the two sides of the top cover (41) are provided with the buckle (45) which is rotatably connected.

5. A novel cell culture well plate according to claim 4, characterized in that the bottom of the culture plate (1) is provided with a fixedly connected bottom plate (3), and both sides of the bottom plate (3) are provided with limiting plates (31) which are matched with the buckles (45).

6. A novel cell culture well plate according to claim 5, characterized in that the other two sides of the bottom plate (3) are provided with finger grooves (32), and the four corners of the bottom plate (3) are provided with fixedly connected legs (33).

7. The novel cell culture well plate according to claim 1, characterized in that the sealing plug (43) is provided with a rubber sealing pad, and the knob (44) is provided with anti-slip teeth.