CN210560492U

CN210560492U - Stem cell layered co-culture device

Info

Publication number: CN210560492U
Application number: CN201921460922.5U
Authority: CN
Inventors: 秦松辉
Original assignee: Shanghai Lianheng Biotechnology Co ltd
Current assignee: Shanghai Lianheng Biotechnology Co ltd
Priority date: 2019-09-04
Filing date: 2019-09-04
Publication date: 2020-05-19
Anticipated expiration: 2029-09-04

Abstract

The utility model discloses a device that stem cell layering coculture, including a plurality of culture dishes and a plurality of ware lid of arranging with the culture dish, its characterized in that: the culture dish storage device is characterized by also comprising a plurality of supports, wherein a culture dish storage opening is formed in each support, an elastic ring is arranged on the inner wall of each culture dish storage opening, and a plurality of storage openings are formed in each elastic ring; the inner walls of the elastic sheets are provided with annular slots, annular magnetic sheets are fixed in the slots, culture dishes are placed in the storage openings, and the culture dishes are tangent to the inner walls of the storage openings; a plurality of elastic sheets are fixed at the bottom of the culture dish, and the elastic sheets are inserted into the slots and are adsorbed and fixed with the magnetic sheets; the bottom of the elastic ring is provided with a plurality of connecting holes communicated with the slots. The utility model has simple structure, the culture dishes can be stacked up and down, the transportation is convenient, and the layered co-culture of stem cells is convenient; when the culture dish is used, the stability is strong, and the culture dish is not easy to slide off the workbench; when the culture dish is transported, collision is not easy to occur, and the culture dish is not easy to break.

Description

Stem cell layered co-culture device

Technical Field

The utility model relates to a device for layered co-culture of stem cells.

Background

Stem cells are a type of pluripotent cells that have the ability to self-replicate. Under certain conditions, it can differentiate into a variety of functional cells.

At present, stem cells are often required to be cultured in a laboratory and are generally placed in a culture dish. A petri dish is a laboratory vessel for the cultivation of microorganisms or cells, consisting of a flat disc-shaped bottom and a lid, generally made of glass or plastic. The culture dish is basically made of two types, mainly plastic and glass, and the glass can be used for plant materials, microorganism culture and adherent culture of animal cells. The plastic may be of polyethylene material, disposable and reusable, suitable for laboratory inoculation, streaking, bacterial isolation and may be used for the cultivation of plant material.

The culture dish has some defects in the use process. When placing on the desktop or when placing on the workstation, the easy landing from the workstation of unstability, the problem of breach and fracture appears easily when the collision because of using glass material in the adipose-derived stem cell culture dish.

Disclosure of Invention

The utility model aims to solve the technical problem that a stem cell layered co-culture device can solve the problems.

The utility model discloses a realize through following technical scheme: a device for layered co-culture of stem cells comprises a plurality of culture dishes, a plurality of dish covers matched with the culture dishes and a plurality of supports, wherein a culture dish storage opening is formed in each support, an elastic ring is arranged on the inner wall of each culture dish storage opening, and a plurality of storage openings are formed in each elastic ring; the inner walls of the elastic sheets are provided with annular slots, annular magnetic sheets are fixed in the slots, culture dishes are placed in the storage openings, and the culture dishes are tangent to the inner walls of the storage openings; a plurality of elastic sheets are fixed at the bottom of the culture dish, and the elastic sheets are inserted into the slots and are adsorbed and fixed with the magnetic sheets; the bottom of the elastic ring is provided with a plurality of connecting holes communicated with the slots.

Preferably, the bracket is a plate-shaped structure made of hard plastic or aluminum alloy.

As the preferred technical scheme, the culture dish and the dish cover are in cylindrical structures; a culture cavity is arranged on the culture dish; the outer side of the culture dish is provided with an external thread, the inner side of the dish cover is provided with an internal thread, and the culture dish and the dish cover are meshed through the threads; the culture dish and the dish cover are made of high-light-transmission glass; the bottom of the dish cover is bonded with a sealing rubber layer.

As the preferred technical scheme, the elastic ring is made of rubber and is fixedly bonded on the inner wall of the culture dish storing opening.

As the preferred technical scheme, the elastic sheet is made of elastic metal, and the elastic sheet, the magnetic sheet and the culture dish are bonded and fixed.

The utility model has the advantages that: the utility model has simple structure, the culture dishes can be stacked up and down, the transportation is convenient, and the layered co-culture of stem cells is convenient; when the culture dish is used, the stability is strong, and the culture dish is not easy to slide off the workbench; when the culture dish is transported, collision is not easy to occur, and the culture dish is not easy to break.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a top view of the present invention;

fig. 2 is a schematic structural view of the stacked structure of the present invention.

Detailed Description

All of the features disclosed in this specification, or all of the steps in any method or process so disclosed, may be combined in any combination, except combinations of features and/or steps that are mutually exclusive.

Any feature disclosed in this specification (including any accompanying claims, abstract and drawings), may be replaced by alternative features serving equivalent or similar purposes, unless expressly stated otherwise. That is, unless expressly stated otherwise, each feature is only an example of a generic series of equivalent or similar features.

In the description of the present invention, it is to be understood that the terms "one end", "the other end", "the outside", "upper", "inside", "horizontal", "coaxial", "central", "end", "length", "outer end", etc. indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, in the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

The use of terms herein such as "upper," "above," "lower," "below," and the like in describing relative spatial positions is for the purpose of facilitating description to describe one element or feature's relationship to another element or feature as illustrated in the figures. The spatially relative positional terms may be intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "below" or "beneath" other elements or features would then be oriented "above" the other elements or features. Thus, the exemplary term "below" can encompass both an orientation of above and below. The device may be otherwise oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly

In the present invention, unless otherwise explicitly specified or limited, the terms "set", "coupled", "connected", "penetrating", "plugging", and the like are to be understood in a broad sense, and may be, for example, fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

As shown in fig. 1 and 2, the culture dish comprises a plurality of culture dishes 1, a plurality of dish covers 2 matched with the culture dishes, and a plurality of supports 3, wherein a culture dish storage opening is formed in each support, an elastic ring 4 is arranged on the inner wall of the culture dish storage opening, and a plurality of storage openings 401 are formed in each elastic ring; the inner walls of the elastic sheets are provided with annular slots, annular magnetic sheets 5 are fixed in the slots, the magnetic sheets are strong magnets, the culture dishes 1 are placed in the storage port 401, and the culture dishes 1 are tangent to the inner walls of the storage port 401; a plurality of elastic sheets 6 are fixed at the bottom of the culture dish, and the elastic sheets 6 are inserted into the slots and are fixedly adsorbed with the magnetic sheets; the bottom of the elastic ring 4 is provided with a plurality of connecting holes 7 communicated with the slots.

Wherein, the bracket 3 is a plate-shaped structure made of hard plastic or aluminum alloy.

Wherein, the culture dish 1 and the dish cover 2 are in cylindrical structures; a culture cavity is arranged on the culture dish; the outer side of the culture dish is provided with an external thread, the inner side of the dish cover is provided with an internal thread, the culture dish and the dish cover are meshed through the threads, the dish cover and the culture dish are easy to disassemble, and the culture dish and the dish cover are made of highly transparent glass; and a sealing rubber layer 8 is bonded at the bottom of the dish cover, and the sealing performance of the sealing rubber layer is improved.

Wherein, the elastic ring 4 is made of rubber and is bonded and fixed on the inner wall of the culture dish storing opening.

The elastic sheet 6 is made of elastic metal, such as copper, and is bonded and fixed with the magnetic sheet and the culture dish.

The working principle is as follows: during the use, can stack support 3 from top to bottom in proper order, realize culture dish 1 layering coculture operation, culture dish 1 places in proper order in support 3, and elastic ring 4 on the support has played the limiting displacement to the culture dish, and the elastic ring is made for rubber, has increased the shock resistance in transit. The culture dish is not easy to collide and damage in the transportation process.

When a single support 3 is used, the support 3 can be placed on the workbench, and the elastic ring on the support is in contact with the workbench, so that the stability during placement is improved.

During the use, as shown in fig. 2, can stack for a set of two supports, it is fixed to make two supports adsorb from top to bottom through the strong magnetism on the elastic ring, is convenient for hold between the fingers the removal, has the space between two supports, is difficult for extrudeing the damage when holding between the fingers the removal in the laboratory.

The utility model has the advantages that: the device can stack culture dishes up and down, and is convenient for layered co-culture of stem cells; a plurality of culture dishes are fixed in one bracket, so that a plurality of tests can be performed simultaneously; when the culture dish is used, the stability is strong, the culture dish is not easy to slide off the workbench and is not easy to damage; the collision is difficult for the culture dish, and the culture dish is difficult for taking place the damage.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the creative work should be covered within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope defined by the claims.

Claims

1. The utility model provides a device that stem cell layering coculture, includes a plurality of culture dishes and a plurality of dish lid with culture dish collocation, its characterized in that: the culture dish storage device is characterized by also comprising a plurality of supports, wherein a culture dish storage opening is formed in each support, an elastic ring is arranged on the inner wall of each culture dish storage opening, and a plurality of storage openings are formed in each elastic ring; the inner walls of the elastic sheets are provided with annular slots, annular magnetic sheets are fixed in the slots, culture dishes are placed in the storage openings, and the culture dishes are tangent to the inner walls of the storage openings; a plurality of elastic sheets are fixed at the bottom of the culture dish, and the elastic sheets are inserted into the slots and are adsorbed and fixed with the magnetic sheets; the bottom of the elastic ring is provided with a plurality of connecting holes communicated with the slots.

2. The device for the layered co-culture of stem cells according to claim 1, wherein: the bracket is a plate-shaped structure made of hard plastic or aluminum alloy.

3. The device for the layered co-culture of stem cells according to claim 1, wherein: the culture dish and the dish cover are cylindrical structures; a culture cavity is arranged on the culture dish; the outer side of the culture dish is provided with an external thread, the inner side of the dish cover is provided with an internal thread, and the culture dish and the dish cover are meshed through the threads; the culture dish and the dish cover are made of high-light-transmission glass; the bottom of the dish cover is bonded with a sealing rubber layer.

4. The device for the layered co-culture of stem cells according to claim 1, wherein: the elastic ring is made of rubber and is fixedly bonded on the inner wall of the culture dish storage opening; the bottom of the elastic ring is provided with anti-slip textures.

5. The device for the layered co-culture of stem cells according to claim 1, wherein: the elastic sheet is made of elastic metal, and the elastic sheet, the magnetic sheet and the culture dish are bonded and fixed.