CN212504919U - Multi-layer circular cell culture dish - Google Patents
Multi-layer circular cell culture dish Download PDFInfo
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- CN212504919U CN212504919U CN202020447941.0U CN202020447941U CN212504919U CN 212504919 U CN212504919 U CN 212504919U CN 202020447941 U CN202020447941 U CN 202020447941U CN 212504919 U CN212504919 U CN 212504919U
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Abstract
The utility model relates to a cell culture technical field, in particular to circular cell culture dish of multilayer. The multi-layer circular cell culture dish comprises a multi-layer circular cell culture dish main body 1 and a spiral cover 2; the multi-layer circular cell culture dish main body 1 comprises a top layer provided with a spiral cover opening 3, a middle layer provided with a liquid inlet channel opening 4 on each layer, a bottom layer and a side wall. The utility model adopts the appearance design of the cylinder main body, the multiple groups of culture surfaces in the main body have small volume (the diameter of 10-15 cm), which is basically equal to the stacking of a plurality of traditional single-layer culture dishes, does not occupy too much space of the incubator, and has simpler operation and stronger sterility; has no operation dead angle, is convenient for uniformly inoculating cells and completely harvesting the cells, and is suitable for small and medium-scale cell culture tests.
Description
Technical Field
The utility model relates to a cell culture technical field, in particular to circular cell culture dish of multilayer.
Background
In the development of cell products, it is often necessary to perform small and medium-scale cell culture assays, and cell culture vessels are the basic devices for such assays. The common cell culture containers mainly comprise traditional single-layer cell culture dishes/bottles (such as 100mm cell culture dishes/T75 cell culture bottles and the like), and commercial multi-layer cell culture containers (such as T175 multi-layer cell culture bottles and the like). Traditional cell culture dish only has a culture chamber, uses traditional monolayer cell culture dish to enlarge the cell culture when experimental, needs the quantity of greatly increased traditional monolayer cell culture dish, and operating personnel carries out cell inoculation or cell harvest one by one, has consumptive material/human cost increase, complex operation, problem that work efficiency is low.
At present, most of commercialized multilayer cell culture containers are designed for large-scale cell culture, and part of the containers needs to be matched with corresponding cell inoculation or harvesting equipment to achieve the optimal use effect. For small and medium scale cell culture assays, the following limitations exist: the volume and the occupied area are large, the operation in a clean bench is inconvenient, and the space of an incubator is occupied; due to the polygonal design of the cuboid, operation dead angles exist, and uneven cell inoculation or incomplete cell harvesting is easy to cause. CN 110283725A's patent as 20190927 publication discloses a novel multilayer adherent cell culture container structure and CN206157170U multilayer cell culture dish that 20170510 publication, improves on the basis of T75 or T175 cell culture bottle, adopts the main part appearance of cuboid, and the main part is inside to set up multiunit culture surface, and the main part top layer contains 2 spiral lids (contains the ventilated membrane) and 2 inlet channels.
Therefore, for small and medium-scale cell culture tests, a cell culture vessel which can improve the work efficiency and is convenient for personnel to operate is needed to solve the problems.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model provides a circular cell culture dish of multilayer. The utility model discloses a combine traditional cell culture dish small and exquisite (10-15cm diameter), the appearance design of cylinder to and the multi-layer culture characteristics of commercialization multilayer cell culture container, both can improve the work efficiency of medium and small-scale cell culture, also be convenient for personnel's operation, save space.
In order to realize the purpose of the utility model, the utility model provides a following technical scheme:
the utility model provides a multilayer circular cell culture dish, which comprises a multilayer circular cell culture dish main body 1 and a spiral cover 2; the multi-layer circular cell culture dish main body 1 comprises a top layer provided with a spiral cover opening 3, a middle layer provided with a liquid inlet channel opening 4 on each layer, a bottom layer and a side wall.
Preferably, the screw cap 2 is provided with a gas permeable membrane 5.
Preferably, the inlet openings 4 are provided at the edges of the intermediate layer.
Preferably, the inlet opening 4 is of circular design.
Preferably, the edge of the inlet channel opening 4 is provided with a baffle 6.
Preferably, the baffle plate arranged at the edge of the liquid inlet channel opening 4 is 0.2-1.0 cm high.
Preferably, the number of intermediate layers is 2 to 10.
Preferably, the number of the middle layers is 3-5.
In the embodiment provided by the utility model, the number of piles in intermediate level is 4 layers.
Preferably, the inlet openings 4 of each layer of the middle layer are vertically arranged.
Preferably, the diameter of the multi-layer circular cell culture dish main body 1 is 10-15cm, and the height between every two layers is 1.5-3 cm; the diameter of the liquid inlet channel opening 4 is 1.5-2.5 cm; the thickness of each layer of the multi-layer circular cell culture dish main body 1 is 0.15-0.25 cm, and the thickness of the side wall is 0.05-0.20 cm.
In the specific embodiment provided by the utility model, the diameter of the multi-layer circular cell culture dish main body 1 is 15cm, and the height between each layer is 2 cm; the diameter of the liquid inlet channel opening 4 is 2 cm; each layer of the multi-layer circular cell culture dish body 1 is 0.2cm thick, and the side wall thickness is 0.1 cm.
The utility model provides a circular cell culture dish of multilayer. The multi-layer circular cell culture dish comprises a multi-layer circular cell culture dish main body 1 and a spiral cover 2; the multi-layer circular cell culture dish main body 1 comprises a top layer provided with a spiral cover opening 3, a middle layer provided with a liquid inlet channel opening 4 on each layer, a bottom layer and a side wall. The utility model discloses circular cell culture dish of multilayer has following advantage:
(1) the appearance design of a cylindrical main body is adopted, a plurality of groups of culture surfaces in the main body are small in size (the diameter is 10-15 cm), the volume is basically equal to that of a plurality of traditional single-layer culture dishes, excessive space of a culture box is not occupied, the operation is simpler and more convenient, and the sterility is stronger;
(2) has no operation dead angle, is convenient for uniformly inoculating cells and completely harvesting the cells, and is suitable for small and medium-scale cell culture tests.
Drawings
FIG. 1 is a schematic view of the structure of a multi-layer circular cell culture dish according to example 1;
note: the multi-layer round cell culture dish comprises a multi-layer round cell culture dish main body 1, a spiral cover 2, a spiral cover opening 3, a liquid inlet channel opening 4, a breathable film 5 and a baffle 6.
Detailed Description
The utility model discloses a circular cell culture dish of multilayer, this text content can be referred to the technical staff in the field, and proper improvement technological parameter realizes. It is expressly noted that all such substitutions and modifications which are obvious to a person skilled in the art are deemed to be included in the present invention. While the methods and applications of the present invention have been described in terms of preferred embodiments, it will be apparent to those skilled in the art that variations and modifications, or appropriate alterations and combinations, may be made to the methods and applications described herein without departing from the spirit and scope of the invention as defined by the appended claims.
The utility model provides a circular cell culture dish of multilayer for small and medium-scale cell culture. The structure of the multi-layer round cell culture dish comprises:
1. a multi-layer circular cell culture dish main body (comprising a top layer, a middle layer and a bottom layer);
2. a multi-layer round cell culture dish screw cover (comprising a breathable film);
3. and a liquid inlet channel of the multi-layer round cell culture dish.
Wherein:
the main body is designed in a tightly connected integrated manner, a plurality of groups of multi-layer culture surfaces are arranged in the main body at equal intervals from top to bottom and can be divided into a top layer, a middle layer and a bottom layer, and the top layer is provided with a screw cover containing a breathable film;
the screw cap is arranged on the top layer of the main body, and a breathable film is arranged inside the screw cap;
the liquid inlet channel starts from the cover opening of the screw cover and leads to the bottom layer of the main body, and the liquid inlet channel is hollow but the inner walls of the liquid inlet channel are not completely connected together.
The materials used in the multi-layer circular cell culture dish of the utility model can be purchased from the market.
The present invention will be further explained with reference to the following examples:
example 1
As shown in fig. 1, the multi-layer circular cell culture dish comprises a multi-layer circular cell culture dish body 1 and a screw cap 2;
the multi-layer circular cell culture dish main body 1 comprises a top layer provided with a spiral cover opening 3, 4 middle layers each provided with a liquid inlet channel opening 4, a bottom layer and a side wall;
the screw cap 2 is provided with a breathable film 5.
The liquid inlet channel openings 4 are designed in a circular shape and are arranged at the edge of the middle layer, and the liquid inlet channel openings 4 of each layer of the middle layer are vertically arranged. The edge of the liquid inlet passage opening 4 is provided with a baffle 6, and the height of the baffle is 0.5 cm.
The diameter of the multi-layer circular cell culture dish main body 1 is 15cm, and the height between each layer is 2 cm; the diameter of the liquid inlet channel opening 4 is 2 cm; each layer of the multi-layer circular cell culture dish body 1 is 0.2cm thick, and the side wall thickness is 0.1 cm.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A multi-layer circular cell culture dish is characterized by comprising a multi-layer circular cell culture dish main body (1) and a spiral cover (2); the round cell culture dish main body (1) comprises a top layer provided with a spiral cover opening (3), and a middle layer, a bottom layer and a side wall, wherein each layer is provided with a liquid inlet channel opening (4).
2. The multi-layer circular cell culture dish according to claim 1, wherein the screw cap (2) is provided with a gas permeable membrane (5).
3. The multi-layer circular cell culture dish according to claim 1, wherein the inlet channel opening (4) is arranged at the edge of the middle layer.
4. Multilayer circular cell culture dish according to claim 1, characterized in that the inlet channel mouth (4) is of circular design.
5. The multi-layer circular cell culture dish according to claim 1, wherein the edge of the inlet channel opening (4) is provided with a baffle (6).
6. The multi-layer circular cell culture dish of claim 5, wherein the baffle is 0.2-1.0 cm high.
7. The multi-layer circular cell culture dish according to claim 1, wherein the number of the middle layers is 2 to 10.
8. The multi-layer circular cell culture dish according to claim 1, wherein the number of the middle layers is 3 to 5.
9. The multi-layer circular cell culture dish according to claim 1, wherein the inlet openings (4) of each layer of the middle layer are vertically arranged.
10. The multi-layer circular cell culture dish according to claim 1, wherein the diameter of the multi-layer circular cell culture dish body (1) is 10-15cm, and the height between each layer is 1.5-3 cm; the diameter of the liquid inlet channel opening (4) is 1.5-2.5 cm; each layer of the multi-layer circular cell culture dish main body (1) is 0.15-0.25 cm thick, and the side wall thickness is 0.05-0.20 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020447941.0U CN212504919U (en) | 2020-03-31 | 2020-03-31 | Multi-layer circular cell culture dish |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020447941.0U CN212504919U (en) | 2020-03-31 | 2020-03-31 | Multi-layer circular cell culture dish |
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| Publication Number | Publication Date |
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| CN212504919U true CN212504919U (en) | 2021-02-09 |
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| CN202020447941.0U Active CN212504919U (en) | 2020-03-31 | 2020-03-31 | Multi-layer circular cell culture dish |
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