CN210736790U - A novel shake bottle for adherent cell culture - Google Patents
A novel shake bottle for adherent cell culture Download PDFInfo
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- CN210736790U CN210736790U CN201920913594.3U CN201920913594U CN210736790U CN 210736790 U CN210736790 U CN 210736790U CN 201920913594 U CN201920913594 U CN 201920913594U CN 210736790 U CN210736790 U CN 210736790U
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Abstract
The utility model discloses a novel shake bottle for adherent cell culture contains ventilative lid and the body of shaking the bottle, shake the bottle body and contain integrated into one piece's bottleneck, body and bottle end, ventilative lid is established or is inserted and establish on shaking the bottleneck of bottle body, its design characteristics are: an isolating device with holes is fixedly arranged in the bottle body of the shake flask body, and a plurality of carriers for adherent cell culture are filled between the isolating device with holes and the bottom of the shake flask body. The utility model has the advantages of convenient operation, it is simple, use manpower sparingly, the pollution risk is little, high dissolved oxygen, high pass is supported, high mass transfer, has filled internal small and medium-scale market blank, has broken the monopoly of foreign relevant product, satisfies relevant customer demand.
Description
Technical Field
The utility model relates to a shake bottle belongs to cell culture technical field, and specific saying so relates to a novel shake bottle for adherent cell culture.
Background
The cell culture shake flasks on the market at present are mainly used for culture of suspension cells, but not directly used for culture of adherent cells. This is mainly because adherent growth belongs to high density culture, and usually needs to solve the problem of cell growth environment, including large adherent area and good nutrient transfer and mass transfer environment.
At present, for some industries for cell therapy, gene therapy and the like, small-scale (100mL-2L) and high-density adherent culture devices are generally required. In the current domestic market, the small and medium-scale cell high-density culture devices are few, most of the devices are mainly completed by square bottles, spinner bottles or cell factories and the like, but the devices can not provide high-density culture, have complicated operation, high pollution risk, large occupied space volume and higher comprehensive cost, and in addition, in the cell growth process, the static culture process is adopted, the nutrient transfer effect is poorer, so that the environment for the overall growth of cells is poorer; under the condition, many domestic enterprises are forced to buy high-price imported products, which invisibly increases the cost of the pharmaceutical enterprises, and further leads to the increase of the price of the biological medicines.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a novel shake bottle for adherent cell culture for solve the problem in the background art.
In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a novel shake bottle for adherent cell culture, contains ventilative lid and shake a bottle body, shake a bottle body and contain integrated into one piece's bottleneck, body and bottle end, ventilative lid is covered or is inserted and is established shake on the bottleneck of bottle body shake a bottle body internal stability of bottle body has a foraminiferous isolating device and shake and fill between the bottle end of bottle body and be used for the carrier of adherent cell culture.
Furthermore, a plurality of paddle type baffle plates are fixedly arranged on one surface of the bottle bottom of the bottle body facing the isolating device with the holes.
Furthermore, the paddle type baffles are uniformly and fixedly arranged in the middle of one surface of the bottle bottom surface of the bottle shaking body facing the isolating device with the holes in an annular shape.
Furthermore, the isolating device with holes is a circular isolating plate with holes or a polygonal isolating plate with holes.
Furthermore, a plurality of through holes are uniformly distributed on the circular partition plate with holes or the polygonal partition plate with holes, and the diameter of each through hole is smaller than the outer diameter of each carrier; the purpose of this is: the convenience is when changing cell culture liquid, only can let the culture solution pass through, and not let the carrier pass through, reduces the contaminated risk of cell.
Further, the ventilation cover is in threaded cover connection with the bottle opening of the shake flask body.
Further, the ventilation cover is inserted on the bottle mouth of the shake flask body in a threaded manner.
Further, a plurality of air holes are uniformly distributed on the air permeable cover, and each air hole has a diameter of 0.2 um.
Further, the section of the bottle mouth of the shake flask body is circular, and the shape of the bottle mouth is a straight cylinder shape or a flaring shape; the cross section of the bottle neck of the shake flask body is circular, and the shape of the bottle neck is a straight cylinder shape or a bell mouth shape; the section of the bottle body of the shake flask body is circular or polygonal, and the appearance of the bottle body is straight cylinder type or conical type; the section of the bottle bottom of the shake flask body is circular or polygonal, and the shape of the shake flask body is basin-shaped.
Compared with the prior art, the utility model discloses an advantage and beneficial effect are:
1. fills the domestic blank of small and medium-scale markets, breaks the monopoly of foreign related products and meets the requirements of related customers;
2. the related operations such as liquid changing and the like are convenient and simple, labor is saved, and the operation pollution risk is reduced, namely the risk that the cells are polluted because the cells in the shake flask need to be put into a centrifugal device for operation when the liquid needs to be changed after the cells are cultured for a certain time in the traditional shake flask is reduced; after the cells are cultured for a certain time and the liquid needs to be changed, the novel shake flask only needs to directly suck or pour out the culture solution and then add fresh culture solution, so that the operation is very simple and the pollution risk is low;
3. compare with shaking the bottle with volumetric, adopt the utility model provides a when the novel shake bottle carries out cell culture, can provide several times and tens times or even better surface area for cell adherence production, can provide the output of cell greatly.
Drawings
FIG. 1 is a first embodiment of the present invention;
FIG. 2 is a schematic illustration of the shake flask body of FIG. 1 without the gas permeable cover;
FIG. 3 is a cross-sectional view taken along line A-A of FIG. 1;
FIG. 4 is a top view of the perforated separator of FIG. 1;
FIG. 5 is a schematic view showing a state of use of the first embodiment;
fig. 6 shows a second embodiment of the present invention;
FIG. 7 is a schematic illustration of the shake flask body of FIG. 6 without the gas permeable cover;
fig. 8 is a third embodiment of the present invention;
FIG. 9 is a schematic illustration of the shake flask body of FIG. 8 without the gas permeable cover;
fig. 10 is a fourth embodiment of the present invention;
FIG. 11 is a schematic illustration of the shake flask body of FIG. 10 without the gas permeable cover;
fig. 12 shows a fifth embodiment of the present invention;
FIG. 13 is a schematic illustration of the shake flask body of FIG. 12 without the gas permeable cover;
fig. 14 shows a fifth embodiment of the present invention;
FIG. 15 is a schematic illustration of the shake flask body of FIG. 14 without the gas permeable cover;
description of reference numerals:
1. a ventilation cover; 2. a shake flask body; 2.1, opening of the bottle; 2.2, a bottleneck; 2.3, a bottle body; 2.4, bottle bottom; 3. a perforated isolation device; 4. a carrier; 5. a paddle type baffle; 6. a culture solution; 7. shaking table.
Detailed Description
In order to make the technical means, the creation features, the achievement purposes and the functions of the present invention easy to understand and understand, how to implement the present invention is further explained below with reference to the accompanying drawings and the detailed description.
Example 1:
as shown in fig. 1 and fig. 2, the novel shake flask for adherent cell culture provided in this embodiment includes a gas-permeable cover 1 and a shake flask body 2, the shake flask body 2 includes an integrally formed bottleneck 2.1, a bottleneck 2.2, a body 2.3 and a bottom 2.4, the gas-permeable cover 1 is screwed on the bottleneck 2.1 of the shake flask body 2, a porous isolation device 3 is fixedly disposed in the body 1.3 of the shake flask body 2, and a plurality of carriers 4 for adherent cell culture are filled between the porous isolation device 3 and the bottom 2.4 of the shake flask body 2.
Specifically, in this embodiment, as shown in fig. 3, four paddle type baffles 5 are further fixed on a side of the bottom 2.4 of the shake flask body 2 facing the isolating device 3 with holes, and the four paddle type baffles 5 are uniformly fixed in a ring shape in the middle of the side of the bottom 2.4 of the shake flask body 2 facing the isolating device 3 with holes.
More specifically, in the present embodiment, as shown in fig. 4, the perforated separating device 3 is a perforated circular separating plate, a plurality of through holes are uniformly distributed on the perforated circular separating plate, and the diameter of each through hole is smaller than the outer diameter of each carrier 4;
the purpose of the perforated spacer 3 is to: one is to allow only the culture solution 6 to pass through and not the carrier 4 when the cell culture solution 6 is changed, so that the carrier 4 loaded with adherent cells is trapped in the shake flask body 2, thereby reducing the risk of contamination of the cells, and the other is to facilitate the solution changing operation.
More specifically, in the present embodiment, a plurality of air holes are uniformly distributed on the air permeable cover 1, and the diameter of each air hole is 0.2 um;
the purpose of the provision of the venting cover 1 is: on one hand, external air is convenient to enter the shake flask body 2 to be mixed with the culture solution 6, and on the other hand, impurities such as external dust are prevented from entering the shake flask body 2 to pollute the cell production environment and reduce the survival rate of cells.
More specifically, in the present embodiment, the cross sections of the bottle mouth 2.1, the bottle neck 2.2, the bottle body 2.3 and the bottle bottom 2.4 of the shake flask body 2 are all circular; the bottle mouth 2.1, the bottle neck 2.2 and the bottle body 2.3 are all in a straight cylinder shape, and the bottle bottom 2.4 is in a circular basin shape.
More specifically, in this embodiment, the outer diameter of the bottle mouth 2.1 is smaller than the inner diameter of the ventilation cover 1, an external thread which is in threaded fit with the inner wall of the ventilation cover 1 is arranged on the outer wall of the bottle mouth 2.1, and an internal thread which is in threaded fit with the external thread on the outer wall of the bottle mouth 2.1 is arranged on the inner wall of the ventilation cover 1.
More specifically, in the present embodiment, the outer diameter of the ventilation cover 1 is smaller than the outer diameter of the bottle body 2.3 and larger than the outer diameters of the bottle mouth 2.1 and the bottle neck 2.2, and the inner diameters of the bottle mouth 2.1 and the bottle neck 2.2 are equal and smaller than the inner diameters of the bottle body 2.3 and the bottle bottom 2.4.
More specifically, in the present embodiment, the carrier 4 for adherent cell culture is a sheet-like carrier which is developed by the present company, and the specific structure of the sheet-like carrier is disclosed in the patent of "disposable fixed bed apparatus for cell culture" with publication number CN207294807U applied by the present company, and thus the detailed structure thereof is not described again.
When the novel shake flask for adherent cell culture provided by the embodiment is used for adherent cell culture, the operation process is specifically as follows:
(1) unscrewing the ventilation cover 1 from the shake flask body 2, then filling culture solution 6 required by adherent cell culture into the shake flask from a bottle opening 2.1 of the shake flask body 2, and screwing the ventilation cover 1 again when the filled culture solution 6 reaches a required dosage (namely, as shown in figure 5, the liquid level of the culture solution 6 is positioned above the isolating device 3 with holes);
(2) placing the shake flask filled with the culture solution 6 in the step (1) on a shaking table 7;
(3) switching on a power supply of the shaking table 7, starting the shaking table 7 to work, and driving the shaking bottle to shake through the shaking table 7; wherein, in the process that the shaking flask shakes along with the shaking table 7, the culture solution 3 and the carrier 4 in the shaking flask body 2 can also rotate along with the shaking flask; when the culture solution 3 in the shake flask body 2 meets the paddle type baffle 5 at the bottom of the flask 2.4, the flowing direction of the culture solution 3 is changed, namely the culture solution 3 runs along the paddle of the paddle type baffle 5 and the rotation direction of the shake flask, so that the mixing effect of the culture solution 3 and the carrier 4 in the shake flask body 2 is enhanced, the oxygen transferring and mass transferring effects are better, meanwhile, the air entering the shake flask body 2 through the ventilating cover 1 can be rapidly mixed with the culture solution 3 at the liquid level of the culture solution 3, and the dissolved oxygen effect in the culture solution 3 is increased.
(4) After the cells are cultured for a certain time and the liquid needs to be changed, the culture liquid 3 in the shake flask body 2 is discharged in a direct sucking or pouring mode, and then fresh culture liquid is added, so that the liquid can be changed;
(5) repeating the step (4) for multiple times according to needs to complete the culture of adherent cells, and finally separating the cultured adherent cells from the attached carrier 4 by trypsin or collagenase and culturing the adherent cells in a new culture bottle.
Example 2:
as shown in fig. 6 and 7, the present example provides a novel shake flask for adherent cell culture, which is different from example 1 in that: the appearance of the bottle body 2.3 of the shake flask body 2 is conical, the outer diameter of the end, close to the bottle neck 2.2, of the bottle body 2.3 is smaller than that of the ventilation cover 1, and the outer diameter of the end, close to the bottle bottom 2.4, of the bottle body is larger than that of the ventilation cover 1.
Example 3:
as shown in fig. 8 and fig. 9, the present example provides a novel shake flask for adherent cell culture, which is different from example 1 in that: the ventilation cover 1 is inserted on the bottle mouth 2.1 of the shake flask body 2 in a threaded insertion mode; specifically, in this embodiment, the outer diameter of the end of the vent cap 1 inserted into the bottle mouth 2.1 is smaller than the inner diameter of the bottle mouth 2.1, the outer wall of the end of the vent cap 1 inserted into the bottle mouth 2.1 is further provided with an external thread (not shown in the figure) engaged with the bottle mouth 2.1 of the shake flask body 2 in a threaded manner, and the inner wall of the bottle mouth 2.1 is provided with an internal thread (not shown in the figure) engaged with the external thread on the outer wall of the vent cap 1 in a threaded manner.
Example 4:
as shown in fig. 10 and fig. 11, the present example provides a novel shake flask for adherent cell culture, which is different from example 3 in that: the bottle mouth 2.1 is flared.
Example 5:
as shown in fig. 12 and fig. 13, the present example provides a novel shake flask for adherent cell culture, which is different from example 1 in that: the outer diameter of the air-permeable cover 1 is equal to the outer diameter of the bottle body 2.3, and the inner diameters of the bottle mouth 2.1, the bottle neck 2.2, the bottle body 2.3 and the bottle bottom 2.4 are equal.
Based on the above examples 1 to 5 and combining with practical needs, the utility model provides a novel shake flask for adherent cell culture, wherein:
the ventilation cover 1 can be arranged on the bottle mouth 2.1 of the shake flask body 2 in other covering or inserting modes, for example, one end of the ventilation cover is hinged, and the other end of the ventilation cover is buckled on the bottle mouth 2.1;
the shape of the bottle mouth 2.1 can also be designed into other regular or irregular shapes, such as a lotus leaf edge shape;
the profile of the neck 2.2 can also be designed in other regular or irregular shapes, for example, a bell-mouth shape, as shown in fig. 14 and 15, example 6;
the cross section of the bottle body 2.3 can be designed into a polygon, such as a square or a rectangle, etc., and the shape can be designed into a polygon prism or a polygon pyramid, such as a cube, a cuboid, or a pyramid, etc.;
the cross section of the bottle bottom 2.4 can also be designed into a polygon, such as a quadrangle or a pentagon, etc.;
the perforated separating device 3 can also be designed into perforated polygonal partition plates, such as perforated quadrilateral partition plates or perforated pentagonal partition plates, and the like, according to the change of the section form of the bottle body 2.3;
other commercially available carriers that can be used for adherent cell culture can also be used for the carrier 4.
Finally, the above description is only a few embodiments of the present invention, and not to limit the scope of the present invention, all the equivalent structures or equivalent processes of the similar shake flask container made by the contents of the specification and the attached drawings are used for conversion, or directly or indirectly applied to other related technical fields, and all the same principles are included in the protection scope of the present invention.
Claims (9)
1. The utility model provides a novel shake bottle for adherent cell culture, contains ventilative lid (1) and shake bottle body (2), shake bottle body (2) and contain integrated into one piece's bottleneck (2.1), bottleneck (2.2), body (2.3) and bottle end (2.4), ventilative lid (1) is covered or is inserted and is established shake on bottle body (2) the bottleneck (2.1), its characterized in that: an isolating device (3) with holes is fixedly arranged in a bottle body (1.3) of the shake flask body (2), and a plurality of carriers (4) for adherent cell culture are filled between the isolating device (3) with holes and a bottle bottom (2.4) of the shake flask body (2).
2. A novel shake flask for adherent cell culture according to claim 1, wherein: and a plurality of paddle type baffles (5) are fixedly arranged on one surface of the bottle bottom (2.4) of the bottle body (2) facing the isolating device (3) with the holes.
3. A novel shake flask for adherent cell culture according to claim 2, wherein: the paddle type baffles (5) are uniformly and fixedly arranged in the middle of one surface of the bottle bottom (2.4) of the bottle shaking body (2) facing the isolating device (3) with the holes in an annular shape.
4. A novel shake flask for adherent cell culture according to claim 2 or 3, wherein: the isolating device (3) with holes is a round partition plate with holes or a polygonal partition plate with holes.
5. A novel shake flask for adherent cell culture according to claim 4, wherein: a plurality of through holes are uniformly distributed on the circular partition plate with holes or the polygonal partition plate with holes, and the diameter of each through hole is smaller than the outer diameter of each carrier (4).
6. A novel shake flask for adherent cell culture according to claim 1, wherein: the ventilation cover (1) is in threaded cover connection with a bottle mouth (2.1) of the shake flask body (2).
7. A novel shake flask for adherent cell culture according to claim 1, wherein: the ventilation cover (1) is inserted on the bottle mouth (2.1) of the shake flask body (2) in a threaded manner.
8. A novel shake flask for adherent cell culture according to claim 1 or 6 or 7, wherein: a plurality of air holes are uniformly distributed on the air permeable cover (1), and the diameter of each air hole is 0.2 um.
9. A novel shake flask for adherent cell culture according to claim 1, wherein: the section of a bottle mouth (2.1) of the shake flask body (2) is circular, and the appearance is a straight cylinder type or a flaring type; the section of a bottleneck (2.2) of the shake flask body (2) is circular, and the shape of the shake flask body is a straight cylinder shape or a bell mouth shape; the section of a bottle body (2.3) of the shake flask body (2) is circular or polygonal, and the appearance is straight cylinder type or conical type; the section of the bottle bottom (2.4) of the shake flask body (2) is circular or polygonal, and the shape is basin-shaped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920913594.3U CN210736790U (en) | 2019-06-18 | 2019-06-18 | A novel shake bottle for adherent cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920913594.3U CN210736790U (en) | 2019-06-18 | 2019-06-18 | A novel shake bottle for adherent cell culture |
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| Publication Number | Publication Date |
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| CN210736790U true CN210736790U (en) | 2020-06-12 |
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| CN201920913594.3U Active CN210736790U (en) | 2019-06-18 | 2019-06-18 | A novel shake bottle for adherent cell culture |
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