CN215365805U

CN215365805U - Cell culture bottle

Info

Publication number: CN215365805U
Application number: CN202121819258.6U
Authority: CN
Inventors: 唐英杰; 张戌懿
Original assignee: Suzhou Hengkang Life Science Co ltd
Current assignee: Suzhou Hengkang Life Science Co ltd
Priority date: 2021-08-05
Filing date: 2021-08-05
Publication date: 2021-12-31
Anticipated expiration: 2031-08-05

Abstract

The utility model relates to a cell culture bottle, which comprises a culture bottle body, wherein the culture bottle body comprises an upper bottle body and a lower bottle body, a bottle mouth is fixedly connected at the center of the top of the upper bottle body, the bottle mouth is in threaded connection with a bottle cap, a first through hole is formed in the front side of the upper bottle body, a second through hole is formed in the first through hole and is symmetrical relative to the center line of the upper bottle body, a first mounting plate is fixedly connected to one side of the lower bottle body, a second mounting plate is fixedly connected to the other side of the lower bottle body, a first connecting piece is fixedly connected to one side of the first mounting plate, and a second connecting piece is fixedly connected to one side of the second mounting plate. Through setting up the intake pipe be convenient for to the inside oxygen that carries of blake bottle for the reproduction of cell.

Description

Cell culture bottle

Technical Field

The utility model relates to a culture bottle, in particular to a cell culture bottle, and belongs to the technical field of cell culture.

Background

Devices for culturing cells and tissues in vitro are a common type of biological experimental and production devices; conventional in vitro cell culture, particularly mammalian cell culture, is typically performed in fixed tissue culture flasks or spinner flasks, and the cells are cultured using culture flasks with regular replacement of the medium, removal of waste products and collection of useful products.

The bottle lid that need frequently to open when taking a sample to the cell of current blake bottle takes a sample, easily cause the pollution to the inside cell of blake bottle, bottle mouth department has the residue when just taking a sample, the clearance of not being convenient for, the practicality is relatively poor, and current blake bottle when cultivateing the cell, be not convenient for to the inside oxygen of carrying of blake bottle, influence the reproductive rate of cell, when cultivateing a large amount of cells, need a plurality of blake bottles, when stacking each other between a plurality of blake bottles and placing, easily take place to empty and slide even, be unfavorable for cell culture.

SUMMERY OF THE UTILITY MODEL

The present invention is directed to solve the above problems and provides a cell culture bottle, in which a fixing column is disposed inside a culture bottle body to facilitate cell propagation inside the culture bottle body, a rubber sampling bag is disposed to facilitate cell sampling and observation inside the culture bottle body, a bottle cap does not need to be opened repeatedly to prevent cell contamination, and an air inlet pipe is disposed to facilitate oxygen delivery to the inside of the culture bottle body to accelerate cell propagation.

The cell culture bottle comprises a culture bottle body, wherein the culture bottle body comprises an upper bottle body and a lower bottle body, a bottle mouth is fixedly connected to the center of the top of the upper bottle body, a bottle cover is in threaded connection with the bottle mouth, a first through hole is formed in the front side of the upper bottle body, a second through hole is formed in the symmetrical position of the first through hole relative to the center line of the upper bottle body, a first mounting plate is fixedly connected to one side of the lower bottle body, a second mounting plate is fixedly connected to the other side of the lower bottle body, a first connecting piece is fixedly connected to one side of the first mounting plate, and a second connecting piece is fixedly connected to one side of the second mounting plate.

Preferably, the first connecting piece is provided with a groove, the second connecting piece is a T-shaped convex block, and the T-shaped convex block is clamped with the groove.

Preferably, the inside sealing connection of first through-hole has the intake pipe, intake pipe top fixedly connected with silicon rubber cap, the intake pipe end is located the inside of blake bottle body, the gas pocket is seted up to the intake pipe end.

Preferably, the inside sealing connection of second through-hole has the sampling tube, sampling tube one end threaded connection has rubber sample package, the sampling tube other end is located the inside of blake bottle body.

Preferably, the tail end of the sampling tube is of a needle point-shaped structure, a first micro valve is fixedly connected to the sampling tube, and a second micro valve is fixedly connected to the top of the rubber sampling bag.

Preferably, the center of the bottom of the culture bottle body is fixedly connected with a bulge, fixing columns are arranged on two sides of the bulge, and the fixing columns are fixedly connected with the inner wall of the culture bottle body.

The utility model has the beneficial effects that:

1. according to the utility model, the first connecting piece and the second connecting piece are clamped and connected, so that a plurality of culture bottle bodies can be conveniently connected, the phenomenon that the culture bottle bodies are mutually overlapped and toppled when the number of the culture bottle bodies is too large is avoided, oxygen or other liquid can be conveniently conveyed into the culture bottle bodies through the air inlet pipe, a good environment is improved for cell propagation, and a silicon rubber cap is arranged at the top of the air inlet pipe, so that a worker can directly inject oxygen or liquid into the air inlet pipe through a needle cylinder, and the silicon rubber cap has good elasticity, can be repeatedly used and is not easy to leak;

2. according to the utility model, the rubber sampling bag is arranged to facilitate sampling observation of cells in the culture bottle body, the first micro valve and the second micro valve can be opened during sampling, the rubber sampling bag is repeatedly kneaded to enable the samples to enter the rubber sampling bag, the first micro valve and the second micro valve can be closed after sampling is finished, so that the pollution in the culture bottle body is avoided, the rubber sampling bag can be separated from the sampling tube by screwing the rubber sampling bag, so that a worker can conveniently analyze and observe the samples in the rubber sampling bag, and the fixing column is arranged in the culture bottle body to facilitate the mixing effect of the fixing column when the worker shakes the culture bottle body, so that cell propagation is accelerated.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is a schematic view of the connection between the first connecting member and the second connecting member according to the present invention.

FIG. 3 is a schematic view showing the internal structure of the flask body according to the present invention.

Reference numbers in the figures: 1. culturing a bottle body; 2. a bottle cap; 3. a first mounting plate; 4. a second mounting plate; 5. a first connecting member; 6. a second connecting member; 7. an air inlet pipe; 8. a silicone rubber cap; 9. air holes; 10. a sampling tube; 11. rubber sampling bag; 12. a first micro valve; 13. a second micro valve; 14. a protrusion; 15. and (5) fixing the column.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.

Referring to fig. 1-3, a cell culture bottle comprises a culture bottle body 1, wherein the culture bottle body 1 comprises an upper bottle body and a lower bottle body, a bottle mouth is fixedly connected to the center of the top of the upper bottle body, a bottle cap 2 is connected to the bottle mouth in a threaded manner, a first through hole is formed in the front side of the upper bottle body, a second through hole is formed in the symmetrical position of the first through hole relative to the center line of the upper bottle body, a first mounting plate 3 is fixedly connected to one side of the lower bottle body, a second mounting plate 4 is fixedly connected to the other side of the lower bottle body, a first connecting piece 5 is fixedly connected to one side of the first mounting plate 3, and a second connecting piece 6 is fixedly connected to one side of the second mounting plate 4.

As a preferred technical scheme of the utility model, the first connecting piece 5 is provided with a groove, the second connecting piece 6 is a T-shaped convex block, the T-shaped convex block is clamped with the groove, the first connecting piece 5 and the second connecting piece 6 are clamped, so that the plurality of culture bottle bodies 1 can be conveniently connected, and the phenomenon of toppling caused by mutual superposition when the number of the culture bottle bodies 1 is too large is avoided.

As a preferred technical scheme of the present invention, an air inlet pipe 7 is hermetically connected inside the first through hole, a silicone rubber cap 8 is fixedly connected to the top of the air inlet pipe 7, the end of the air inlet pipe 7 is located inside the culture bottle body 1, an air hole 9 is formed at the end of the air inlet pipe 7, the air inlet pipe 7 is arranged to facilitate oxygen or other liquids to be delivered into the culture bottle body 1, so as to provide a good environment for cell propagation, the silicone rubber cap 8 is arranged at the top of the air inlet pipe 7 to facilitate a worker to directly inject oxygen or liquids into the air inlet pipe 7 with a syringe, and the silicone rubber cap 8 has good elasticity, can be repeatedly used, and is not prone to leakage.

As a preferred technical scheme of the utility model, the inside of the second through hole is hermetically connected with a sampling tube 10, one end of the sampling tube 10 is in threaded connection with a rubber sampling package 11, the other end of the sampling tube 10 is positioned inside the culture flask body 1, the tail end of the sampling tube 10 is in a needle-point-shaped structure, a first micro valve 12 is fixedly connected to the sampling tube 10, a second micro valve 13 is fixedly connected to the top of the rubber sampling package 11, the rubber sampling package 11 is arranged to facilitate sampling and observation of cells inside the culture flask body 1, the first micro valve 12 and the second micro valve 13 can be opened during sampling, the rubber sampling package 11 is repeatedly kneaded to enable the sample to enter the inside of the rubber sampling package 11, the first micro valve 12 and the second micro valve 13 can be closed after sampling is finished to avoid the inside of the culture flask body 1 from being polluted, the rubber sampling package 11 can be unscrewed to separate from the sampling tube 10, the staff is convenient for carry out analysis observation to the inside sample of rubber sample package 11.

As a preferred technical scheme of the utility model, the center of the bottom of the culture bottle body 1 is fixedly connected with a bulge 14, two sides of the bulge 14 are provided with fixing columns 15, the fixing columns 15 are fixedly connected with the inner wall of the culture bottle body 1, and the fixing columns 15 are arranged in the culture bottle body 1, so that when a worker shakes the culture bottle body 1, the fixing columns 15 can play a role in mixing, and cell propagation is accelerated.

When the utility model is used, the first connecting piece 5 and the second connecting piece 6 are clamped and connected, so that a plurality of culture bottle bodies 1 can be conveniently connected, the phenomenon that the culture bottle bodies 1 are mutually overlapped and toppled when the number of the culture bottle bodies is too large is avoided, oxygen or other liquid can be conveniently conveyed into the culture bottle bodies 1 through the air inlet pipe 7, a good environment is improved for cell reproduction, the silicon rubber cap 8 is arranged at the top of the air inlet pipe 7, so that a worker can directly inject oxygen or liquid into the air inlet pipe 7 through a needle cylinder, the elasticity of the silicon rubber cap 8 is good, the silicon rubber cap can be repeatedly used and is not easy to leak, the rubber sampling bag 11 is arranged so that cells in the culture bottle bodies 1 can be conveniently sampled and observed, the first micro valve 12 and the second micro valve 13 can be opened when sampling is carried out, the rubber sampling bag 11 is repeatedly kneaded so that a sample can enter the rubber sampling bag 11, and the first micro valve 12 and the second micro valve 13 can be closed after sampling is finished, avoid 1 inside contaminated of blake bottle body, twist and move rubber sample package 11 and can make rubber sample package 11 break away from with sampling tube 10, the staff of being convenient for carries out the analysis to the inside sample of rubber sample package 11 and observes, through set up fixed column 15 in 1 inside of blake bottle body and be convenient for the staff when rocking blake bottle body 1, fixed column 15 plays the mixed action for cell breeding.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof, and it is therefore desired that the present embodiments be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, without any reference thereto being construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A cell culture bottle, includes blake bottle body (1), its characterized in that: the culture bottle body (1) comprises an upper bottle body and a lower bottle body, the center of the top of the upper bottle body is fixedly connected with a bottle mouth, the bottle mouth is in threaded connection with a bottle cap (2), a first through hole is formed in the front side of the upper bottle body, a second through hole is formed in the first through hole relative to the center line of the upper bottle body, a first mounting plate (3) is fixedly connected with one side of the lower bottle body, a second mounting plate (4) is fixedly connected with the other side of the lower bottle body, a first connecting piece (5) is fixedly connected with one side of the first mounting plate (3), and a second connecting piece (6) is fixedly connected with one side of the second mounting plate (4).

2. A cell culture flask according to claim 1, wherein: the first connecting piece (5) is provided with a groove, the second connecting piece (6) is a T-shaped convex block, and the T-shaped convex block is clamped with the groove.

3. A cell culture flask according to claim 1, wherein: the inside sealing connection of first through-hole has intake pipe (7), intake pipe (7) top fixedly connected with silicon rubber cap (8), intake pipe (7) end position is inside culture flask body (1), gas pocket (9) are seted up to intake pipe (7) end.

4. A cell culture flask according to claim 1, wherein: the inside sealing connection of second through-hole has sampling tube (10), sampling tube (10) one end threaded connection has rubber sample package (11), sampling tube (10) other end is located inside culture flask body (1).

5. A cell culture flask according to claim 4, wherein: the tail end of the sampling tube (10) is of a needle point-shaped structure, a first micro valve (12) is fixedly connected to the sampling tube (10), and a second micro valve (13) is fixedly connected to the top of the rubber sampling bag (11).

6. A cell culture flask according to claim 1, wherein: the bottom center of the culture bottle body (1) is fixedly connected with a bulge (14), fixing columns (15) are arranged on two sides of the bulge (14), and the fixing columns (15) are fixedly connected with the inner wall of the culture bottle body (1).