CN208266191U - A kind of Tissue Culture Flask - Google Patents
A kind of Tissue Culture Flask Download PDFInfo
- Publication number
- CN208266191U CN208266191U CN201820578025.3U CN201820578025U CN208266191U CN 208266191 U CN208266191 U CN 208266191U CN 201820578025 U CN201820578025 U CN 201820578025U CN 208266191 U CN208266191 U CN 208266191U
- Authority
- CN
- China
- Prior art keywords
- tissue culture
- culture flask
- partition
- bottle body
- lower chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000005192 partition Methods 0.000 claims abstract description 22
- 239000012528 membrane Substances 0.000 claims abstract description 17
- 238000000502 dialysis Methods 0.000 claims abstract description 12
- 238000007789 sealing Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 9
- 238000003760 magnetic stirring Methods 0.000 claims description 8
- 239000004793 Polystyrene Substances 0.000 claims description 6
- 229920002223 polystyrene Polymers 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 abstract description 12
- 239000002699 waste material Substances 0.000 abstract description 7
- 238000009825 accumulation Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 239000012530 fluid Substances 0.000 abstract description 3
- 238000001802 infusion Methods 0.000 abstract description 3
- 230000002503 metabolic effect Effects 0.000 abstract description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 230000002146 bilateral effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Abstract
The utility model provides a kind of Tissue Culture Flask, including bottle body, bottle body inner horizontal is provided with a partition, partition will be separated into upper cavity and lower chamber inside bottle body, the side surface upper part of upper cavity is provided with upper cavity open tubes, the side surface upper part of lower chamber is provided with lower chamber open tubes, and upper cavity open tubes and lower chamber open tubes are provided with sealing cover or ventilating cover, are provided with dialysis membrane in partition.Tissue Culture Flask provided by the utility model reduces the accumulation of metabolic waste concentration, improves the appreciation rate and cell activity of cell, guarantees that cell culture environment is good, while being avoided that operational pollution caused by multiple fluid infusion, saves the time and efforts of laboratory technician.
Description
Technical field
The utility model relates to a kind of Tissue Culture Flask more particularly to a kind of good cell trainings of guarantee cell culture environment
Support bottle.
Background technique
Existing cell culture in the fields such as biology and medicine, research, treatment etc. during need to various cells into
Row culture, the environmental demand of different cell culture is all different, and the equipment for cell culture is also different, including culture dish, training
Bottle, culture test tube, incubator etc. are supported, the Cell culture bottle structure of the prior art is simple, cannot be to the cell and its environment of culture
Play preferable protective effect.
Biology laboratory generally can all use cell culture bags when carrying out suspension cell large-scale culture, currently on the market
Common cell culture bags require experimenter's periodically manual supplementing culture medium, more time-consuming and laborious；And it is common in the market
Experiment suspending cell culture bottle is all bilateral arm type, can only constantly supplement fresh culture into culture bottle, and waste liquid and generation
Thanking to product cannot be discharged.In addition, pollution is also easy to produce during the supplemented medium into culture bottle, once culture medium is dirty
Dye, the cell that directly will lead in Tissue Culture Flask are not available, cause damages.
Utility model content
The technical problems to be solved in the utility model is to overcome existing defect, provides a kind of Tissue Culture Flask, is guaranteed thin
Born of the same parents' culture environment is good.
In order to solve the above-mentioned technical problem, the utility model provides the following technical solution:
A kind of Tissue Culture Flask, including bottle body, the bottle body inner horizontal are provided with a partition, and the partition will be described
Upper cavity and lower chamber are separated into inside bottle body, the side surface upper part of the upper cavity is provided with upper cavity open tubes, the cavity of resorption
The side surface upper part of body is provided with lower chamber open tubes, and the upper cavity open tubes and lower chamber open tubes are provided with sealing cover or saturating
Gas lid is provided with dialysis membrane in the partition.
Further, the bottle body is made of polystyrene, and the inner wall of the bottle body is handled by TC.
Further, the partition is made of support plate and dialysis membrane, and the support plate is made of polystyrene and table
Face is handled by TC, and the pore size of the dialysis membrane is 0.2-0.65 nanometers, and the area of the dialysis membrane accounts for the partition table
The 50-70% in face.
Further, the longitudinal section of the partition is V-arrangement.
Further, the ventilating cover is provided with every moist membrane liner, it is described be provided with every moist membrane liner it is several
The aperture of 0.2 micron pore size.
Further, the side bottom of the lower chamber is provided with threeway liquid flowing tube.
Further, top opening is provided at the top of the bottle body, the top opening is provided with seal rubber plug.
It further, further include magnetic stirring bar, the upper inner liner of the magnetic stirring bar has rubber washer.
A kind of Tissue Culture Flask of the utility model reduces the accumulation of metabolic waste concentration, improves the appreciation rate and cell of cell
Activity guarantees that cell culture environment is good, while being avoided that operational pollution caused by multiple fluid infusion, save laboratory technician time and
Energy.
Detailed description of the invention
Attached drawing is used to provide a further understanding of the present invention, and constitutes part of specification, practical with this
Novel embodiment is used to explain the utility model together, does not constitute limitations of the present invention.In the accompanying drawings:
Fig. 1 is the structural schematic diagram of the utility model.
Specific embodiment
It is illustrated below in conjunction with preferred embodiment of the attached drawing to the utility model, it should be understood that described herein excellent
It selects embodiment to be only used for describing and explaining the present invention, is not used to limit the utility model.
As shown in Figure 1, a kind of Tissue Culture Flask, including bottle body 1,1 inner horizontal of bottle body are provided with a partition 2, partition 2
Upper cavity and lower chamber will be separated into inside bottle body 1, the side surface upper part of upper cavity is provided with upper cavity open tubes 3, lower chamber
Side surface upper part is provided with lower chamber open tubes 4, and upper cavity open tubes 3 and lower chamber open tubes 4 are provided with sealing cover 5, in partition
It is provided with dialysis membrane 6.
Bottle body 1 is made of polystyrene, and chemical corrosion resistance is good, and the inner wall of bottle body is handled by TC, improves cell
Adsorption capacity.
Partition 2 is made of support plate and dialysis membrane 6, and support plate is made of polystyrene and surface is handled by TC, thoroughly
The pore size for analysing film is 0.2-0.65 nanometers, and the area of dialysis membrane accounts for the 50-70% on partition surface.Guarantee that waste liquid smoothly permeates
While to lower chamber, also guarantee that cell there are enough attachment surfaces.
The longitudinal section of partition 2 is V-arrangement, increases the surface area of partition, the molded non-planars such as arc surface can also be made.
Sealing cover 5 also could alternatively be ventilating cover, and ventilating cover is provided with every moist membrane liner, sets every moist membrane liner
It is equipped with the aperture of several 0.2 micron pore sizes, it is possible to provide continue sterile gas exchanges, while preventing from polluting.
The side bottom of lower chamber is provided with threeway liquid flowing tube 7, convenient for emptying the waste liquid in lower chamber.
The top of bottle body 1 is provided with top opening, and top opening is provided with seal rubber plug 8, it is ensured that the leakproofness of bottle body.
This Tissue Culture Flask further includes magnetic stirring bar 9, and extracting rubber plug can be inserted into magnetic stirring bar 9 in bottle body, magnetic force
The upper inner liner of stirring rod 9 has rubber washer 10, it is ensured that the leakproofness of bottle body after magnetic stirring bar insertion.
Working principle:
Suspension cell culture (by taking K562 cell as an example)
1, the Tissue Culture Flask after sterilizing is taken out, is placed in superclean bench.
2, the K562 cell after recovery is taken out, cell suspension is added in culture bottle from upper cavity open tubes with liquid-transfering gun,
It adds at 1640 culture mediums to 3/4ths of entire bottle body containing 10% fetal calf serum and (does not exceed upper cavity open tubes under
Cavity open tubes position).
3, seal rubber plug is opened, magnetic stirring bar is inserted into, is placed on matching used magnetic stirring apparatus, moves into carbon dioxide
It is cultivated in incubator.
4, lower chamber open tubes or threeway liquid flowing tube are opened when changing liquid, covers tightly spiral cover after pouring out the waste liquid in culture bottle.It beats
Upper cavity open tubes are opened, are added into 1640 culture mediums containing 10% fetal calf serum.
5, when collecting cell, upper cavity open tubes is opened, the cell suspension in culture bottle is poured out, be centrifuged, collect cell.
A kind of Tissue Culture Flask of the utility model reduces the accumulation of metabolic waste concentration, improves the appreciation rate and cell of cell
Activity guarantees that cell culture environment is good, while being avoided that operational pollution caused by multiple fluid infusion, save laboratory technician time and
Energy.
Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention, it is not limited to this
Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art
For, it is still possible to modify the technical solutions described in the foregoing embodiments, or to part of technical characteristic
It is equivalently replaced.Within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on,
It should be included within the scope of protection of this utility model.
Claims (8)
1. a kind of Tissue Culture Flask, it is characterised in that: including bottle body, the bottle body inner horizontal is provided with a partition, described
Partition will be separated into upper cavity and lower chamber inside the bottle body, the side surface upper part of the upper cavity is provided with upper cavity opening
Pipe, the side surface upper part of the lower chamber are provided with lower chamber open tubes, the upper cavity open tubes and the setting of lower chamber open tubes
There are sealing cover or ventilating cover, is provided with dialysis membrane in the partition.
2. a kind of Tissue Culture Flask according to claim 1, it is characterised in that: the bottle body is made of polystyrene,
The inner wall of the bottle body is handled by TC.
3. a kind of Tissue Culture Flask according to claim 1, it is characterised in that: the partition is by support plate and dialysis membrane group
At the support plate is made of polystyrene and surface is handled by TC, and the pore size of the dialysis membrane is 0.2-0.65
Nanometer, the area of the dialysis membrane account for the 50-70% on the partition surface.
4. a kind of Tissue Culture Flask according to claim 1, it is characterised in that: the longitudinal section of the partition is V-arrangement.
5. a kind of Tissue Culture Flask according to claim 1, it is characterised in that: the ventilating cover is provided with every moist film
Liner, the aperture that several 0.2 micron pore sizes are provided with every moist membrane liner.
6. a kind of Tissue Culture Flask according to claim 1, it is characterised in that: the side bottom of the lower chamber is provided with
Threeway liquid flowing tube.
7. a kind of Tissue Culture Flask according to claim 1, it is characterised in that: be provided with top at the top of the bottle body and open
Mouthful, the top opening is provided with seal rubber plug.
8. a kind of Tissue Culture Flask according to claim 7, it is characterised in that: it further include magnetic stirring bar, the magnetic force
The upper inner liner of stirring rod has rubber washer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820578025.3U CN208266191U (en) | 2018-04-20 | 2018-04-20 | A kind of Tissue Culture Flask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820578025.3U CN208266191U (en) | 2018-04-20 | 2018-04-20 | A kind of Tissue Culture Flask |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208266191U true CN208266191U (en) | 2018-12-21 |
Family
ID=64680432
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820578025.3U Expired - Fee Related CN208266191U (en) | 2018-04-20 | 2018-04-20 | A kind of Tissue Culture Flask |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208266191U (en) |
-
2018
- 2018-04-20 CN CN201820578025.3U patent/CN208266191U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181221 |