CN218755730U - Laboratory is with simple and easy solid state fermentation device - Google Patents

Abstract

The application relates to a simple solid state fermentation device for a laboratory, which comprises a box body, a detachable cover body and a liquid drainage device arranged outside the box body, wherein the liquid drainage device comprises a liquid suction pipe, an air bag power assembly, a liquid drainage pipe and a collecting bottle; the pipette penetrates through the cover body and is positioned in the box body, and the lower port of the pipette is arranged close to the bottom of the box body; the lower port of the liquid discharge pipe is positioned in the collecting bottle, and the lower port of the liquid discharge pipe is positioned above the lower port of the liquid suction pipe; the air bag power assembly comprises a flow dividing pipe, an air inlet pipe and an air bag, and two ends of the air inlet pipe and the flow dividing pipe are respectively communicated with the liquid suction pipe and the liquid discharge pipe; the air bag is communicated with the air inlet pipe, a one-way valve is arranged between the air inlet pipe and the liquid suction pipe, and a water stop clamp is arranged on the shunt pipe. The fermentation liquid collecting device has the advantages of simple structure, convenience in operation and difficulty in causing environmental pollution when collecting fermentation liquid.

Description

Laboratory is with simple and easy solid state fermentation device

Technical Field

The application relates to the technical field of biological fermentation, in particular to a simple solid-state fermentation device for a laboratory.

Background

Solid state fermentation refers to a method for culturing microorganisms by using an insoluble solid substrate, and compared with liquid state fermentation, the solid state fermentation has the advantages of low water activity, easy growth of microorganisms, convenient operation and the like, and has an irreplaceable position in the field of microbial fermentation. The solid state fermentation device comprises a dynamic solid state fermentation device and a static solid state fermentation device, wherein the dynamic solid state fermentation device comprises a reactor with mechanical stirring or a rotary drum, and the static solid state fermentation device comprises a shallow disc type or tower column type reactor. Generally, fungi grow better in a static environment, and secondary metabolites accumulate more efficiently; however, when the culture system is relatively large, the static solid state fermentation device has a problem that the metabolic heat is difficult to remove. Therefore, the static solid state fermentation device is more suitable for the laboratory scale use with a smaller culture system.

At present, the solid state fermentation devices specially provided for laboratories are fewer, and generally open trays or shake flasks are mostly adopted, but the open trays have the problem of easy pollution, and the shake flasks have the problems of poor air fluidity and limited sample loading amount. In order to solve the problems, a closed culture box can be adopted for solid state fermentation, the closed culture box generally comprises a box body and a detachable cover body, and the box body or the cover body is provided with a vent hole; when in use, the fermentation substrate and the zymophyte are placed in the box body, and the cover body is covered; then the culture box is placed in an incubator, and air circulation of the culture box is realized by utilizing an air blowing device of the incubator. However, since a large amount of spores generated by solid-state fermentation of fungi are mainly accumulated and adsorbed on the surface of solid-state culture medium particles, the spores released during collection of fermentation liquid easily cause environmental pollution, and thus, the current solid-state fermentation device still has difficulty in meeting the requirements of laboratories.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the application provides a simple and easy solid state fermentation device is used in laboratory, and its device simple structure, easily transport are suitable for the small-scale fermentation culture in laboratory, and closed cultivation and drain's setting both can reduce the entering of fermentation process pollutant, can reduce the fungal spore again and release in the environment.

A simple solid state fermentation device for a laboratory comprises a box body, a detachable cover body and a liquid drainage device arranged outside the box body, wherein the liquid drainage device comprises a liquid suction pipe, an air bag power assembly, a liquid drainage pipe and a collection bottle;

the pipette penetrates through the cover body and is positioned in the box body, and the lower port of the pipette is arranged close to the bottom of the box body;

the lower port of the liquid discharge pipe is positioned in the collecting bottle, and the lower port of the liquid discharge pipe is positioned above the lower port of the liquid suction pipe;

the air bag power assembly comprises a flow dividing pipe, an air inlet pipe and an air bag, and two ends of the air inlet pipe and the flow dividing pipe are respectively communicated with the liquid suction pipe and the liquid discharge pipe; the air bag is communicated with the air inlet pipe, a one-way valve is arranged between the air inlet pipe and the liquid suction pipe, and a water stop clamp is arranged on the shunt pipe.

By adopting the technical scheme, the closed fermentation culture is adopted, so that pollutants can be prevented from entering the fermentation process; through drain's setting, at the cultivation initial stage, can put the stagnant water clamp on the shunt tubes, then pinch the gasbag, during external gas can loop through fluid-discharge tube, gasbag and pipette gets into the lower cavity of box body, then passes filter plate and filter membrane and get into the upper chamber, then discharges through the bleeder vent to the realization is cultivateed the gas exchange of environment. Along with the extension of fermentation time, the fermentation liquor can be stored in the lower chamber, and the fermentation liquor is discharged by adopting a liquid discharging device. Firstly, put the stagnant water clamp on the shunt tubes, then pinch the gasbag, make the gas in gasbag and the fluid-discharge tube discharge under in the cavity, during the zymotic fluid can get into the pipette this moment, then open the stagnant water clamp immediately, because be equipped with the check valve on the intake pipe, the zymotic fluid can't pass through the intake pipe, only can pass through the shunt tubes from the fluid-discharge tube discharge into the receiving flask. The liquid discharge device can realize continuous liquid discharge, is simple to operate and is easy to realize.

Optionally, a filter membrane for filtering the fermentation liquid is arranged in the box body, and the lower port of the pipette is located below the filter membrane.

Optionally, a filter plate for bearing is arranged below the filter membrane in the box body.

By adopting the technical scheme, the filter membrane and the filter plate are arranged to divide the box body into the upper chamber and the lower chamber, the fermentation substrate and the fermentation bacteria are positioned above the filter membrane, and fermentation broth generated by fermentation can enter the lower chamber through the filter membrane and the filter plate, so that the fermentation broth is separated, and then the filtrate is discharged through the liquid discharge device, and the pollution of fungal spores to the environment can be avoided.

Optionally, the cover body is provided with air holes, and a sterile air-permeable film is covered on the cover body above the air holes.

Through adopting above-mentioned technical scheme, in order to keep the humidity in the culture medium, need to open aseptic ventilated membrane this moment to the culture medium moisturizing, it can to use the atomizer to spray the atomizing water in to the box body.

Optionally, the lid is located on the box body, the one end that the pin joint was kept away from to the lid has set firmly the fixture block, articulated on the box body have with fixture block matched with buckle.

Through adopting above-mentioned technical scheme, adopt buckle formula connected mode, the lid of being convenient for open and shut.

Optionally, the box body is a transparent plastic box.

Optionally, a sealing ring for sealing is arranged between the box body and the cover body.

By adopting the technical scheme, the transparent plastic box is adopted, the whole process is visual, and the fermentation condition is easy to control and adjust.

In summary, the present application has the following beneficial effects:

the application provides a laboratory is with simple and easy solid state fermentation device, its device simple structure, easily transport are suitable for the fermentation culture of laboratory small-scale, and the setting of closed cultivation and drain both can reduce the entering of fermentation process pollutant, can reduce fungal spore again and release to the environment in. The liquid discharge device can realize continuous liquid discharge, is simple to operate and is easy to realize.

Drawings

FIG. 1 is a schematic diagram of a simple solid state fermentation apparatus for a laboratory according to the present application.

In the figure: 1. a box body; 11. buckling; 2. a cover body; 21. a clamping block; 22. air holes are formed; 23. a through hole; 3. a seal ring; 4. a sterile, breathable film; 5. filtering a plate; 6. filtering the membrane; 7. a liquid discharge device; 71. a pipette; 72. an air bag power assembly; 721. a shunt tube; 722. an air inlet pipe; 723. an air bag; 724. a one-way valve; 725. a water stop clip; 73. a liquid discharge pipe; 74. and (6) collecting the bottle.

Detailed Description

The present application is further described with reference to the following figures and examples.

The embodiment of the application discloses a laboratory is with simple and easy solid state fermentation device. Referring to fig. 1, solid state fermentation device includes box body 1, and the outer wall of 1 top one side of box body articulates there is lid 2, and the one end that its pin joint was kept away from to lid 2 has set firmly fixture block 21, and it has buckle 11 with fixture block 21 matched with to articulate on the box body 1. A sealing ring 3 for sealing is arranged between the box body 1 and the cover body 2. The cover body 2 is provided with air holes 22, and the cover body 2 is covered with a sterile breathable film 4 above the air holes 22. A filter plate 5 is horizontally arranged at the bottom of the box body 1, and a filter membrane 6 which can pass liquid and gas but can block solid from passing is arranged above the filter plate 5. The filter plate 5 and the filter membrane 6 divide the box body 1 into an upper area and a lower area which are an upper chamber and a lower chamber respectively.

When the box is used, the cover body 2 is opened, and a fermentation substrate and fermentation bacteria are added into the box body 1 and positioned in the upper cavity; then the cover body 2 is buckled, and the sterile breathable film 4 is covered on the breathable holes 22. During the cultivation, the liquid produced by fermentation can pass through the filter membrane 6 and the filter plate 5 into the lower chamber, so that the culture medium can be separated from the fermentation broth. In order to maintain the humidity in the culture medium, water needs to be added to the culture medium, and at this time, the sterile breathable membrane 4 is opened, and atomized water is sprayed into the box body 1 by using an atomizer.

The liquid discharging device 7 is arranged at one side of the solid state fermentation device on the box body 1, and the liquid discharging device 7 comprises a liquid sucking pipe 71, an air bag 723 power component 72, a liquid discharging pipe 73 and a collecting bottle 74; the cover body 2 is provided with a through hole 23, and the pipette 71 passes through the through hole 23 and is positioned in the lower chamber of the cartridge body 1. The lower port of the drain pipe 73 is located in the collection bottle 74, and the lower port of the drain pipe 73 is located above the lower port of the pipette 71.

The air bag 723 power assembly 72 comprises a shunt tube 721, an air inlet tube 722 and an air bag 723; the intake pipe 722 and the bypass pipe 721 are connected at both ends to the suction pipe 71 and the drain pipe 73, respectively. The air bag 723 is communicated with the air inlet pipe 722, and a one-way valve 724 is arranged between the air inlet pipe 722 and the liquid suction pipe 71; a water stop clamp 725 is arranged on the shunt tube 721. At the initial stage of culture, the water-stop clamp 725 is placed on the shunt tube 721, then the air bag 723 is pinched, and outside air can sequentially enter the lower chamber of the box body 1 through the drain tube 73, the air bag 723 and the pipette 71, then pass through the filter plate 5 and the filter membrane 6 into the upper chamber, and then be exhausted through the air vent 22, so that air exchange of the culture environment is realized. Along with the extension of fermentation time, the fermentation liquor can be stored in the lower chamber, and the liquid discharge device 7 is adopted to discharge the fermentation liquor. Firstly, the water-stop clamp 725 is placed on the shunt tube 721, then the air bag 723 is pinched, so that air in the air bag 723 and the liquid discharge pipe 73 is discharged into the lower cavity, at the moment, the fermentation liquid enters the liquid suction pipe 71, then the water-stop clamp 725 is opened immediately, and due to the fact that the check valve 724 is arranged on the air inlet pipe 722, the fermentation liquid cannot pass through the air inlet pipe 722, and only can be discharged into the collection bottle 74 from the liquid discharge pipe 73 through the liquid discharge pipe. Through the drain 7 of this application can realize lasting flowing back, easy operation easily realizes.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims (7)

1. A simple solid state fermentation device for a laboratory comprises a box body (1) and a detachable cover body (2), and is characterized by further comprising a liquid drainage device (7) arranged outside the box body (1), wherein the liquid drainage device (7) comprises a liquid suction pipe (71), an air bag (723), a power assembly (72), a liquid drainage pipe (73) and a collection bottle (74);

the pipette (71) penetrates through the cover body (2) and is positioned in the box body (1), and the lower port of the pipette (71) is arranged close to the bottom of the box body (1);

the lower port of the drain pipe (73) is positioned in the collection bottle (74), and the lower port of the drain pipe (73) is positioned above the lower port of the pipette (71);

the air bag (723) power assembly (72) comprises a shunt pipe (721), an air inlet pipe (722) and an air bag (723), and two ends of the air inlet pipe (722) and the shunt pipe (721) are respectively communicated with a liquid suction pipe (71) and a liquid discharge pipe (73); the air bag (723) is communicated with the air inlet pipe (722), a one-way valve (724) is arranged between the air inlet pipe (722) and the liquid suction pipe (71), and a water stop clamp (725) is arranged on the shunt pipe (721).

2. The simple solid state fermentation device for laboratory according to claim 1, wherein a filter membrane (6) for filtering fermentation liquid is arranged in the box body (1), and the lower port of the pipette (71) is positioned below the filter membrane (6).

3. The simple solid-state fermentation device for the laboratory according to claim 2, wherein a filter plate (5) for bearing is arranged below the filter membrane (6) in the box body (1).

4. The simple solid state fermentation device for laboratories according to claim 1, wherein the lid body (2) is provided with an air vent (22), and a sterile breathable membrane (4) is covered on the lid body (2) above the air vent (22).

5. The simple solid-state fermentation device for the laboratory according to claim 1, wherein the cover body (2) is arranged on the box body (1), a clamping block (21) is fixedly arranged at one end of the cover body (2) far away from the hinge point, and a buckle (11) matched with the clamping block (21) is hinged on the box body (1).

6. The simple solid-state fermentation device for the laboratory according to claim 1, wherein the box body (1) is a transparent plastic box.

7. The simple solid-state fermentation device for the laboratory according to claim 1, wherein a sealing ring (3) for sealing is arranged between the box body (1) and the cover body (2).

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