CN206219611U - A kind of liquid filling device for cell culture bags - Google Patents

A kind of liquid filling device for cell culture bags Download PDF

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Publication number
CN206219611U
CN206219611U CN201621259033.9U CN201621259033U CN206219611U CN 206219611 U CN206219611 U CN 206219611U CN 201621259033 U CN201621259033 U CN 201621259033U CN 206219611 U CN206219611 U CN 206219611U
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China
Prior art keywords
cell culture
culture bags
cylindrical tube
liquid filling
filling device
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Active
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CN201621259033.9U
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Chinese (zh)
Inventor
范星洲
吴明远
李建有
刘洋
刘乐锋
徐志国
郝超
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eastern Union Stem Cell & Gene Engineering Co Ltd
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Eastern Union Stem Cell & Gene Engineering Co Ltd
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Priority to CN201621259033.9U priority Critical patent/CN206219611U/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/14Bags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps

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  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses a kind of liquid filling device for cell culture bags, including support frame, mounting platform is provided with support frame as described above, removably cylindrical tube is provided with the mounting platform, the top of the cylindrical tube is threadedly coupled by the half-thread structure for setting with spiral cover, and the bottom of the cylindrical tube is provided with flow regulator.The utility model can coordinate disposable cell culture bags in cell culture bags between perfusion cultures base or culture bag nutrient solution transfer, present apparatus overall structure is simple, easy to use.

Description

A kind of liquid filling device for cell culture bags
Technical field
The utility model is related to a kind of liquid filling device for cell culture bags.
Background technology
Cell culture bags are a kind of cells that can support suspension cell high-density growth made by special gas permeable material Culture vessel.Compared with conventional flask, cell culture bags gas exchange area is big, can obtain greater number of cell;It is existing Cell culture bags using aseptic PVC pipeline as medium/cells suspension turnover pipeline, closure is strong, can substantially reduce thin The probability that born of the same parents pollute and operating personnel infect, but this also brings inconvenience to laboratory technician to perfusion cultures liquid in culture bag.This reality This problem is solved with new.
Utility model content
The defect that the utility model purpose is directed to prior art presence provides a kind of liquid filling dress for cell culture bags Put.
The utility model to achieve the above object, is adopted the following technical scheme that:A kind of liquid filling dress for cell culture bags Put, including support frame, mounting platform is provided with support frame as described above, removably it is provided with cylindrical tube on the mounting platform Body, the top of the cylindrical tube is threadedly coupled by the half-thread structure for setting with spiral cover, under the cylindrical tube Portion is provided with flow regulator.
Further, the cylindrical tube is made up of glass material, and its bottom is in funnel-form.
Further, being marked on the lateral wall of the cylindrical tube has.
Further, support frame as described above is made up of stainless steel, and it is highly 13-20cm.
Further, it is provided with arrangement for adjusting height on support frame as described above.
Further, the spiral cover is provided with air-vent, and the internal diameter of the air-vent is 0.6cm.
The beneficial effects of the utility model:The utility model can coordinate disposable cell culture bags to be filled in cell culture bags The transfer of nutrient solution between note culture medium or culture bag, present apparatus overall structure is simple, easy to use.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Specific embodiment
Shown in Fig. 1, it is related to a kind of liquid filling device for cell culture bags, including support frame 1, is set on support frame as described above 1 Mounting platform 6 is equipped with, removably cylindrical tube 3, the top of the cylindrical tube 3 are provided with the mounting platform 6 It is threadedly coupled with spiral cover 4 by the half-thread structure 5 for setting, the bottom of the cylindrical tube 3 is provided with flow regulator 7。
Further scheme is that the top of the spiral cover 4 has air-vent 8, and the internal diameter of the air-vent 8 is 0.6cm, can separately be connect 0.22um filters.
Further scheme is that the cylindrical tube 3 is made up of glass material, and its bottom is in funnel-form.So it is easy to Remove cleaning and sterilize, it is reusable.
Further scheme is that there be mark on the lateral wall of the cylindrical tube 3;So can conveniently record Pour into the liquor capacity of culture bag.
Further scheme is that support frame as described above 1 is made up of stainless steel, and it is highly 13-20cm.
Further scheme is that arrangement for adjusting height 2 is provided with support frame as described above 1.
Before using this cell culture bags liquid filling device to liquid is irrigated in cell culture bags, can first by the circle in the device Column-shaped barrel 3 takes out, and is put into the sterilizing of high-pressure sterilizing pot mesohigh, takes out standby after drying.When using, by the cylinder after sterilizing Cylinder 3 is put on support frame 1, is adjusted to extreme lower position, then by cell culture bags support frame 1 by arrangement for adjusting height 2 PVC feed tubes insertion cylindrical tube 3 bottom connector on.Flow regulator 7 is closed, cylindrical tube is then opened The spiral cover 4 at the top of body 3, pours into the liquid for needing volume.Spiral cover 4 is covered, a 0.22um filter is connected on air-vent 8, can Probability is polluted in reduction perfusing course.Support frame 1 is raised by arrangement for adjusting height 2, opens flow regulator 7 to flow Maximum, the liquid in cylindrical tube 3 can be then flowed rapidly into culture bag, can be passed through with one-shot injector after liquid flow is complete 0.22um filters are appropriate to make residual liquid in PVC feed tubes fully enter in culture bag to some air are squeezed into culture bag, subtracts Of low pollution probability.After finishing, cylindrical tube 3 is taken out into cleaning, reusable after sterilizing, last support frame 1 is used After 75% alcohol is wiped over totally, can be placed directly in super-clean bench.
Preferred embodiment of the present utility model is the foregoing is only, is not used to limit the utility model, it is all in this practicality Within new spirit and principle, any modification, equivalent substitution and improvements made etc. should be included in guarantor of the present utility model Within the scope of shield.

Claims (6)

1. a kind of liquid filling device for cell culture bags, it is characterised in that peace is provided with including support frame, on support frame as described above Assembling platform, dismountable on the mounting platform to be provided with cylindrical tube, the top of the cylindrical tube is by setting Half-thread structure is threadedly coupled with spiral cover, and the bottom of the cylindrical tube is provided with flow regulator.
2. a kind of liquid filling device for cell culture bags as claimed in claim 1, it is characterised in that the cylindrical tube By glass material is made, its bottom is in funnel-form.
3. a kind of liquid filling device for cell culture bags as claimed in claim 1, it is characterised in that the cylindrical tube Lateral wall on mark and have.
4. a kind of liquid filling device for cell culture bags as claimed in claim 1, it is characterised in that support frame as described above is not for Rust steel matter is made, and it is highly 13-20cm.
5. a kind of liquid filling device for cell culture bags as described in any one of Claims 1-4, it is characterised in that described Arrangement for adjusting height is provided with support frame.
6. a kind of liquid filling device for cell culture bags as claimed in claim 1, it is characterised in that the spiral cover is provided with Air-vent, the internal diameter of the air-vent is 0.6cm.
CN201621259033.9U 2016-11-23 2016-11-23 A kind of liquid filling device for cell culture bags Active CN206219611U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201621259033.9U CN206219611U (en) 2016-11-23 2016-11-23 A kind of liquid filling device for cell culture bags

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201621259033.9U CN206219611U (en) 2016-11-23 2016-11-23 A kind of liquid filling device for cell culture bags

Publications (1)

Publication Number Publication Date
CN206219611U true CN206219611U (en) 2017-06-06

Family

ID=58790273

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201621259033.9U Active CN206219611U (en) 2016-11-23 2016-11-23 A kind of liquid filling device for cell culture bags

Country Status (1)

Country Link
CN (1) CN206219611U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107586717A (en) * 2017-10-31 2018-01-16 河南省肿瘤医院 A kind of attachment means of cell culture bags and culture jar

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107586717A (en) * 2017-10-31 2018-01-16 河南省肿瘤医院 A kind of attachment means of cell culture bags and culture jar
CN107586717B (en) * 2017-10-31 2024-03-26 河南省肿瘤医院 Connecting device for cell culture bag and culture solution bottle

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