CN207193302U - A kind of cell culture liquid replenisher - Google Patents
A kind of cell culture liquid replenisher Download PDFInfo
- Publication number
- CN207193302U CN207193302U CN201721198838.1U CN201721198838U CN207193302U CN 207193302 U CN207193302 U CN 207193302U CN 201721198838 U CN201721198838 U CN 201721198838U CN 207193302 U CN207193302 U CN 207193302U
- Authority
- CN
- China
- Prior art keywords
- cylinder
- shaped
- falling
- supplementation pipe
- liquid supplementation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
It the utility model is related to a kind of cell culture liquid replenisher, including the cylinder that upper end is open, internal thread is provided with the inside of the upper end of cylinder, the liquid supplementation pipe of falling Y-shaped is provided among the bottom of cylinder, the upper end for the liquid supplementation pipe of falling Y-shaped is connected with cylinder, the non-divergent ends for the liquid supplementation pipe of falling Y-shaped are provided with valve switch, internal thread is provided with the inside of the one of divergent ends for the liquid supplementation pipe of falling Y-shaped, another divergent ends port for the liquid supplementation pipe of falling Y-shaped is provided with air-permeable, hydrophobic membrane, and the thickness of the air-permeable, hydrophobic membrane is 2 μm.The utility model is simple in construction, and fluid infusion is simple to operate, laborsaving, saving of labor, and it is excessive to occur nutrient solution during nutrient solution fluid infusion, takes enclosed fluid infusion mode to avoid nutrient solution and air contact, reduces the possibility of cell contamination.
Description
Technical field
The utility model belongs to technical field of cell culture, more particularly to a kind of cell culture liquid replenisher.
Background technology
Cell culture technology is also cell clone technology, and substantial amounts of cell or its metabolite are obtained by cell culture.
Because biological product is got from cell, it is possible to which it is most crucial, most basic in biotechnology to say cell culture technology
Technology.Cell culture work has been widely used in the every field such as biology, medical science, new drug development, turns into most important base
One of plinth science.The growth of cell needs nutrient environment, for maintaining the nutrient matrix of cell growth to be referred to as culture medium.Culture medium
It can be divided into fluid nutrient medium and solid medium by its physical state.In existing cell cultivation process, it is common to use benefit
Liquid mode is the syringe using syringe, and inversion is put connects cell culture bags in the bracket, topples over nutrient solution toward the inside, this mistake
Cheng Jiyi pours out nutrient solution outside syringe, and this open type fluid infusion mode adds the possibility of cell contamination, causes whole
Individual cell culture fluid is contaminated.
The content of the invention
The purpose of this utility model is to provide for a kind of cell culture liquid replenisher, and it is thin to solve addition in the prior art
The easily contaminated problem of born of the same parents' nutrient solution.
The utility model is adopted the technical scheme that to solve the above problems, there is provided a kind of cell culture fluid infusion
Device, including the cylinder that upper end is open, the upper end inner side of the cylinder are provided with internal thread, Y-shaped are provided among the bottom of cylinder
Liquid supplementation pipe, the upper end for the liquid supplementation pipe of falling Y-shaped are connected with cylinder, and the non-divergent ends for the liquid supplementation pipe of falling Y-shaped are provided with valve switch, fall
Internal thread is provided with the inside of one of divergent ends of Y-shaped liquid supplementation pipe, another divergent ends port for the liquid supplementation pipe of falling Y-shaped is provided with
Air-permeable, hydrophobic membrane, the thickness of the air-permeable, hydrophobic membrane is 2 μm.
Outside is provided with anti-slip veins among the cylinder.
Annular boss is provided with the outside of the upper end of the cylinder.
The beneficial effects of the utility model：The utility model is simple in construction, and fluid infusion is simple to operate, laborsaving, saving of labor,
It is excessive that nutrient solution will not occur during nutrient solution fluid infusion, take enclosed fluid infusion mode to avoid nutrient solution and connect with air
Touch, reduce the possibility of cell contamination.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Embodiment
To make the purpose of this utility model, technical scheme and beneficial effect clearer, below in conjunction with the accompanying drawings to this practicality
New embodiment is described in further detail.
As shown in figure 1, the utility model provides a kind of cell culture liquid replenisher, including the cylinder 1 that upper end is open, cylinder
Be provided with annular boss 6 on the outside of the upper end of body 1, outside is provided with anti-slip veins 5 among cylinder 1, anti-slip veins 5 can strengthen cylinder 1 and
Grip force between palm, the upper end inner side of the cylinder 1 are provided with internal thread, the fluid infusion of falling Y-shaped are provided among the bottom of cylinder 1
Pipe 2, the upper end of the liquid supplementation pipe of falling Y-shaped 2 are connected with cylinder 1, and the non-divergent ends of the liquid supplementation pipe of falling Y-shaped 2 are provided with valve switch 3,
Internal thread, another divergent ends port of the liquid supplementation pipe of falling Y-shaped 2 are provided with the inside of one of divergent ends of the liquid supplementation pipe of falling Y-shaped 2
Provided with air-permeable, hydrophobic membrane 4, the thickness of air-permeable, hydrophobic membrane 4 is 2 μm, and air-permeable, hydrophobic membrane 4 can ensure that air enters the fluid infusion of falling Y-shaped
In pipe 2, and liquid can not can so continue to provide aseptic gas exchange by air-permeable, hydrophobic membrane 4, ensure cell culture fluid
Smoothly flow down.
In use, after the lid of opening culture jar 7, cylinder 1 is directly threadedly attached on culture jar 7, valve is opened
Close 3 to be closed, the interface thread of culture bag is connected in the liquid supplementation pipe of falling Y-shaped 2, the bottleneck court of culture jar 7
Under, then whole device is placed on support and opens valve switch 3 and can be carried out fluid infusion and operate.The utility model structure
Simply, fluid infusion is simple to operate, laborsaving, saving of labor, and it is excessive to occur nutrient solution during nutrient solution fluid infusion, takes envelope
The fluid infusion mode of enclosed avoids nutrient solution and air contact, reduces the possibility of cell contamination.
The advantages of general principle of the present utility model, principal character and the utility model has been shown and described above.One's own profession
The technical staff of industry is it should be appreciated that the utility model is not restricted to the described embodiments, described in above-described embodiment and specification
Simply illustrate principle of the present utility model, the utility model can also on the premise of the spirit and scope of the utility model is not departed from
There are various changes and modifications, these changes and improvements are both fallen within the range of claimed utility model.The requires of the utility model
Protection domain is defined by appended claims and equivalent.
Claims (3)
1. a kind of cell culture liquid replenisher, including the cylinder that upper end is open, it is characterised in that：Set on the inside of the upper end of the cylinder
There is internal thread, the liquid supplementation pipe of falling Y-shaped is provided among the bottom of cylinder, the upper end for the liquid supplementation pipe of falling Y-shaped is connected with cylinder, falls
The non-divergent ends of Y-shaped liquid supplementation pipe are provided with valve switch, and interior spiral shell is provided with the inside of the one of divergent ends for the liquid supplementation pipe of falling Y-shaped
Line, another divergent ends port for the liquid supplementation pipe of falling Y-shaped are provided with air-permeable, hydrophobic membrane, and the thickness of the air-permeable, hydrophobic membrane is 2 μm.
A kind of 2. cell culture liquid replenisher according to claim 1, it is characterised in that：Outside is set among the cylinder
There are anti-slip veins.
A kind of 3. cell culture liquid replenisher according to claim 2, it is characterised in that：Set on the outside of the upper end of the cylinder
There is annular boss.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721198838.1U CN207193302U (en) | 2017-09-19 | 2017-09-19 | A kind of cell culture liquid replenisher |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721198838.1U CN207193302U (en) | 2017-09-19 | 2017-09-19 | A kind of cell culture liquid replenisher |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207193302U true CN207193302U (en) | 2018-04-06 |
Family
ID=61789779
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721198838.1U Active CN207193302U (en) | 2017-09-19 | 2017-09-19 | A kind of cell culture liquid replenisher |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207193302U (en) |
-
2017
- 2017-09-19 CN CN201721198838.1U patent/CN207193302U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204608026U (en) | A kind of cell with super hydrophilic growth surface embeds ware | |
| CN207193302U (en) | A kind of cell culture liquid replenisher | |
| CN206418144U (en) | A kind of permeability type lymphocyte blake bottle | |
| CN108641926A (en) | A kind of microbial fermentation avoids the system and method for microbiological contamination when inoculation | |
| CN206591134U (en) | A kind of Tissue Culture Flask of high leakproofness | |
| CN205182764U (en) | Centrifugal tube | |
| CN106047675B (en) | A kind of medium bottle for being used to prepare blood agar plate | |
| CN204474691U (en) | Cell culture bags special filling liquid funnel | |
| CN203735216U (en) | Anoectochilus formosanus culture bottle | |
| CN204219377U (en) | One determines dosage medicament taking bottle | |
| CN204198747U (en) | A kind of cell culture bags of convenient operation | |
| CN208586299U (en) | A kind of bacteria culture bottle convenient for sampling | |
| CN205313577U (en) | Aseptic cell culture bottle | |
| CN201524289U (en) | Disposable sputum suction device | |
| CN206219611U (en) | A kind of liquid filling device for cell culture bags | |
| CN206760467U (en) | A kind of laboratory Zooplankton cultivating device | |
| CN101597560A (en) | A kind of device that is used for preparing plating medium | |
| CN205170864U (en) | Cell culture bottle | |
| CN201719629U (en) | Sterile syringe | |
| CN206020150U (en) | Carry out the experimental provision that polyacrylamide gel contaminates glue | |
| CN203128570U (en) | Denitrification authentication prefabricated pipe | |
| CN209337191U (en) | A kind of dusting-proof bottle neck | |
| CN203740155U (en) | Traditional chinese medicine filling device | |
| CN208414418U (en) | A kind of living cells transport bottle | |
| CN204276009U (en) | The sealing dropper of band storage liquid function |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |