CN106047675B - A kind of medium bottle for being used to prepare blood agar plate - Google Patents
A kind of medium bottle for being used to prepare blood agar plate Download PDFInfo
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- CN106047675B CN106047675B CN201610677019.9A CN201610677019A CN106047675B CN 106047675 B CN106047675 B CN 106047675B CN 201610677019 A CN201610677019 A CN 201610677019A CN 106047675 B CN106047675 B CN 106047675B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
The invention discloses a kind of medium bottle for being used to prepare blood agar plate, including bottle, bottle outer wall is equipped with graduation mark, the bottle upper end is equipped with respectively the inclined sample-adding bottle neck in both sides to the left and right and topples over bottle neck, it is described to topple over bottle neck equipped with built-in plug core and sealing cover, wherein fill in core and topple over bottle neck inner wall and realize that seamless seal inserts, plug core lower end is equipped with double-deck, convex cambered surface strainer, and plug core upper end is tightened by screw thread and sealing cover；The sample-adding bottle neck is configured with the ventilating cover that capping is evenly distributed with air hole, and ventilating cover is tightened with sample-adding bottle neck by screw thread.Pass through the plug core with double-layer leaching net, liquid blood media surface can be effectively filtered out in bottle because of a large amount of foams caused by quick shake up, so that form smooth, smooth, consistent surface after the blood agar culture-medium solidification poured into, operating efficiency is improved while blood agar culture-medium quality is ensured, Bottle structure design is reasonable, control, can be applicable easy to experimental implementation and experiment parameter.
Description
Technical field
The invention belongs to Experiment on Microbiology fixture technology field, it is related to a kind of medium bottle, refers specifically to one kind and be used to prepare
The medium bottle of blood agar plate.
Background technology
Blood agar culture-medium is a kind of containing de- fiber animal blood（Generally use rabbit blood or sheep blood）Beef extract-peptone training
Base is supported, in addition to containing the culture required various nutrition of bacterium, moreover it is possible to which coenzyme is provided（Such as the V factors）, ferroheme（The X factors) etc. spy
Different growth factor, is usually used in cultivating, separate and preserving some pathogenic microorganisms to nutritional requirement harshness in microbiological test；
In addition, it may also be used for measure the haemocylolysis of bacterium.In view of the rigors that some bacteriums prepare blood agar culture-medium, are such as trained
Sterile de- fiber Sheep Blood need to be proportionally added in 45 DEG C or so insulations after supporting base sterilizing, after fully mixing, pour into sterilizing training
Support ware.If temperature is too high, the blood plate color prepared can black, and certainly will influence result of the test or be not easy to observe bacterium
Haemolysis circle, therefore have to be cooled to required temperature and could add sheep blood.Since business blood agar culture-medium expense is prohibitively expensive,
And used medium has the problems such as preparing overlong time, being limited by the shelf-life, tend not to meet laboratory standard requirement.For
Ensure the time effects that experimental result is prepared from agar plate, save experimentation cost, for this reason, laboratory often needs largely manually to incline
Make blood agar culture-medium by oneself.At present, the preparation of blood agar culture-medium generally uses glass spinner flask, needs the training that will first prepare
Foster based sols are poured into conical flask, 121 DEG C of autoclavings 15 minutes, are cooled to 50 ~ 55 DEG C, sterile working adds 5% ~ 10% (V/
V) the aseptic de-fiber sheep blood of pre-temperature to 37 DEG C, fully mixes, pours into sterilized petri dishes, you can.But during test operation often
There are problems with：（1）During culture medium and de- fiber sheep blood are fully mixed, substantial amounts of floatation of foam can be often produced
In on the liquid level for mixing culture medium, defoamed in the past usually through standing or heating, but with the extension and training of operating time
The decline of base temperature is supported, skewness easily occurs in medium component, or even the phenomenon solidified occurs, mixes again, can produce again
A large amount of foams, foam removal rate are relatively low；When pouring into sterilized petri dishes, float on liquid level foam be poured into culture medium it is flat
In ware, after culture medium solidification, its surface often forms more pit not of uniform size（Lather collapse causes）, can not prepare
Smooth, smooth media surface needed for test requirements document, in later scribes inoculation, painting work, often occurs scratching agar
The phenomenon on surface, influences the accuracy of result of the test.（2）During pouring operation, usually left hand is responsible for taking sterilizing and is put down
Ware, the right hand hold bottle and constantly mix operation, to be further ensured that culture medium and blood reach optimal mixing state.Due to agar culture
The temperature of 50 DEG C or so of holding is needed when base pours into, in a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices stream process, the amount of pour plate is needed quickly, accurately, if inclining
Overlong time is noted, then with the reduction of temperature, agar and de- fiber sheep blood in conical flask will produce the phenomenon of skewness, lead
Nutritional ingredient is uneven between causing agar plate, it is impossible to meets the uniformity of experiment condition, widens the difference of experimental result.（3）At present
The sterilization of culture medium is carried out using conical flask plus the sealing of silica gel plug and tinfoil, there is easy gas leakage rupture of sealing etc. after disinfection and ask
Topic.And use common tool to fill in reagent bottle, since bottleneck is without ventilative design, need release bottleneck to sterilize in high-pressure sterilizing course
Disinfection, easily causes the hidden danger such as culture medium spilling, the built-in storehouse of pollution high voltage steam sterilization pan and water trapping layer.In addition, common tool plug
Bottleneck size, angle of inclination and the bottle cap different design of reagent bottle close ergonomic designs and require, and exist use in operation
The problem of inconvenient.（4）There is the problem of being not easy to grasp in traditional glass conical flask body, in addition conical flask after autoclaving, from
Its body temperature is excessively boiling hot when pressure cooker takes out, and adds grasping difficulty, easily scalds hand, not easy to operate, and increase pours into, dispenses blood
The time of tablet；And bottle temperature need to be controlled in the range of 50 ~ 55 DEG C when pouring into, since operation process is slow, often so that taper
The culture medium not being finished down in bottle pours into blood plate again close to after solidifying, it is necessary to reheat, melt, and thus, adds culture
The probability of base pollution, causes the plating medium temperature poured into inconsistent, be easy to cause culture medium result of the test repeat it is parallel
Otherness is larger.（5）If the culture base unit weight prepared in bottle is excessive, in originally pour plate, it is not easy to control pour into it is each
The amount of culture dish, bottleneck is also easy to produce reflux when toppling over culture medium, easily causes pollution, and operation is time-consuming and laborious, and the blood plate of preparation is difficult
Required with reaching unified standard, cannot be met the requirements when the experiment of culture is compared, there is no preferable solution to this at present
Method.
The content of the invention
, can be quick the present invention provides a kind of medium bottle for being used to prepare blood agar plate for above-mentioned technical problem
Efficiently remove a large amount of foams for mixing culture medium liquid level, it is ensured that operating efficiency, bottle are improved while blood agar culture-medium quality
Body is reasonable in design, is controlled easy to experimental implementation and experiment parameter.
The present invention is achieved through the following technical solutions above-mentioned purpose：
A kind of medium bottle for being used to prepare blood agar plate, including bottle, bottle outer wall are equipped with graduation mark, the bottle
Upper end is equipped with respectively the inclined sample-adding bottle neck in both sides to the left and right and topples over bottle neck, described to topple over bottle neck equipped with built-in plug core and sealing
Lid, wherein plug core realizes that seamless seal inserts with toppling over bottle neck inner wall, plug core lower end is equipped with double-deck, convex cambered surface strainer, fills in
Core upper end is tightened by screw thread and sealing cover；The sample-adding bottle neck is configured with the ventilating cover that capping is evenly distributed with air hole, breathes freely
Lid is tightened with sample-adding bottle neck by screw thread.
As the prioritization scheme of this case, it is described plug core be hollow columnar structures, by upper end pouring opening, middle-end protrusion and
Lower port three parts form integral structure；Wherein, the strainer being fixed with one with it, lower port outer wall are equipped with the inside of lower port
Equipped with silica gel spiral mouth, plug core is by silica gel spiral mouth and topples over the realization sealing inserting of bottle neck inner wall；The middle-end protrusion
Outside diameter is more than the bore for toppling over bottle neck, and its lower face and the bottleneck edge close contact for toppling over bottle neck；The upper end pouring opening
Outer wall is equipped with plug core screw thread, it is tightened with sealing cover by screw thread.
As the prioritization scheme of this case, the bottle outer wall, which is matched somebody with somebody, is arranged with dismountable collet.
As the prioritization scheme of this case, the collet is rectangular sheet structure made of silica gel, vertical the two of collet
Side is correspondingly provided with adhesive strip, is wrapped collet in bottle outer wall by adhesive strip.
As the prioritization scheme of this case, the ventilating cover capping is equipped with the air hole in concentric circles arrangement, in ventilating cover
Side is equipped with fungi-proofing filter membrane and the annular silica gel packing ring with radian, and fungi-proofing filter membrane is supported by annular silica gel packing ring and fixed, ventilating cover
Madial wall is equipped with screw thread, and lateral wall is equipped with the anti-skidding raised line of vertical bar shape.
As the prioritization scheme of this case, the sealing cover inside top is equipped with the circular silica gel pad circle with radian, sealing cover
Madial wall is equipped with screw thread, and lateral wall is equipped with the anti-skidding raised line of vertical bar shape.
As the prioritization scheme of this case, the angle between the central axes of the sample-adding bottle neck and horizontal plane is 40-60 °, is toppled over
Angle between the central axes of bottle neck and horizontal plane is 40-70 °.
As the prioritization scheme of this case, the bottle lower part is in rectangular parallelepiped structure, inside corresponding tilt at left and right sides of top
Sample-adding bottle neck is set respectively in ramped shaped, in the slope surface of slope and topples over bottle neck.
As the prioritization scheme of this case, the angle between bottle slope slope surface and horizontal plane is set to 30-50 °.
As the prioritization scheme of this case, the bottle is made of Pyrex, and plug core main body uses polypropylene or poly- carbon
Acid esters is made, and sealing cover and ventilating cover are made of phenolic resin.
The beneficial effects of the invention are as follows：
（1）By being provided with the plug core of double-layer leaching net structure, liquid blood media surface in bottle can be effectively filtered out
Because of a large amount of foams caused by quick shake up, it is ensured that form smooth, smooth, consistent table after the blood agar culture-medium solidification poured into
Face；Fill in the three stage structure design of core, good airproof performance, the close not water clock of fitting；
（2）The inclined plane on bottle top and the design of both sides tilting adding mouth and pour spout, it is reasonable in design, meet
Ergonomic designs require, easy to which various reagents are added or drawn in culture medium process for preparation so that take liquid or sample-adding operation
More smoothly, it is convenient, avoid touching between vessel polluting；And effectively control and grasp the culture medium poured into plate
Amount, is not likely to produce backflow phenomenon, conveniently pours into, improve the accuracy rate and success rate poured into；
（3）Bottle outer wall sets collet, and on the one hand personnel easy to operation take, grasp the higher bottle of temperature, avoid
Scald；On the other hand the effect of insulation is played, medium bottle is extended and maintains time in optimum temperature range, beneficial to mixed
The operation of even, dumping bottle In vivo culture base and sheep blood, has striven for the time so that culture medium during pour plate to pour into, dispensing
Temperature is consistent, and improves the success rate of culture medium preparation；
（4）The design of air hole and fungi-proofing filter membrane in ventilating cover so that during high-temperature sterilization steam can circulate and bacterium not
Can, reach aseptic storage purpose, and natural, rapid decompression inside bottle can be made after sterilizing, prevent culture medium from overflowing bottleneck；
（5）Sealing cover top inner side sets sealed silicone packing ring, and sealing cover is connected with plug core by thread seal, further
Ensure that culture medium will not overflow bottleneck in high-pressure sterilizing course；
（6）Rational in infrastructure, simple operation, has preferable market prospects and economic benefit, suitable for promoting and applying.
Brief description of the drawings
Fig. 1 is the structure diagram that the present invention prepares blood agar plate medium bottle；
Fig. 2 is the overall structure diagram that the present invention prepares blood agar plate medium bottle；
Fig. 3 is that the present invention prepares the structure diagram that core is filled in blood agar plate medium bottle；
Fig. 4 is the top view that the present invention prepares ventilating cover in blood agar plate medium bottle；
Fig. 5 is the top view that the present invention prepares sealing cover in blood agar plate medium bottle；
Fig. 6 is the structure diagram that the present invention prepares collet in blood agar plate medium bottle；
In figure：1- bottles, 2- ventilating covers, 3- sealing covers, 4- plug cores, 5- sample-adding bottle necks, 6- topple over bottle neck, 7- bottle neck spiral shells
Line, 8- bottle cap internal threads, 9- upper ends pouring opening, 10- middle-end protrusions, 11- lower ports, 12- strainers, 13- silica gel spiral mouths,
14- fills in core screw thread, 15- internal threads, 16- collets, 17- graduation marks, 18- labels, 19- air holes, the fungi-proofing filter membranes of 20-, 21- rings
Shape silicone gasket, the anti-skidding raised lines of 22-, 23- circular silica gel pad circles, 24- antiskid threads, 25- adhesive strips.
Embodiment
The present invention and its effect are further elaborated below in conjunction with accompanying drawings and embodiments.
As shown in Figure 1, 2, a kind of medium bottle for being used to prepare blood agar plate, including bottle 1,1 upper end of bottle
Equipped with the inclined sample-adding in both sides and topples over bottle neck 6 at bottle neck 5 to the left and right respectively, topple over bottle neck 6 and be equipped with supporting built-in plug core 4 and close
Capping 3；Wherein fill in core 4 and topple over 6 inner wall of bottle neck and realize that seamless seal inserts, 4 lower end of plug core is equipped with double-deck, convex cambered surface and filters
Net 12,4 upper end of plug core are tightened by screw thread and sealing cover 3；Sample-adding bottle neck 5 is configured with capping and is evenly distributed with the ventilative of air hole 19
Lid 2, sample-adding 5 upper end outer wall of bottle neck are equipped with bottle neck screw thread 7, and 2 madial wall of ventilating cover is equipped with bottle cap internal thread 8, ventilating cover 2 and sample-adding
Bottle neck 5 is tightened by bottle neck screw thread 7 and bottle cap internal thread 8.
Preferably, as shown in Fig. 3, plug core 4 is by 11 three parts group of upper end pouring opening 9, middle-end protrusion 10 and lower port
Integrated hollow columnar structures；The length of plug core 4 is set to 6.5-8.0 cm, and internal diameter is set to 2.4-3.2 cm.Wherein, lower end
11 length of mouth are set to 3.0-3.5 cm, and the inner side of lower port 11 is equipped with double-deck, the convex cambered surface strainer being fixed with one with it
12, strainer 12 can effectively filter out in bottle liquid blood media surface because of a large amount of foams caused by quick shake up, it is ensured that incline
Smooth, even curface is formed after the blood agar culture-medium solidification of note；11 outer wall of lower port is equipped with silica gel spiral mouth 13, fills in core 4
By silica gel spiral mouth 13 and topple over the realization sealing inserting of 6 inner wall of bottle neck, good airproof performance, the close not water clock of fitting.Middle-end is protruded
The outside diameter in portion 10 is set to 3.8-4.2 cm, and length is set to 2.0-3.0 cm, its outside diameter is more than the bore for toppling over bottle neck 6, and under it
End face and the bottleneck edge close contact for toppling over bottle neck 6, can control the plug insertion of core 4 to tilt the depth of bottle neck 6.Upper end pouring opening 9
Length be set to 2.0-2.5 cm, pouring opening 9 lateral wall in upper end is equipped with plug core screw thread 14, and 3 madial wall of sealing cover is equipped with bottle cap
Screw thread 15, sealing cover 3 are tightened with plug core 4 by filling in core screw thread 14 and bottle cap internal thread 15, when sealing cover 3 and plug core screw thread
After 14 are tightened, no leak source contact can be fully achieved, there is safety leak-proof effect.
Preferably, the sample-adding bottle neck 5 is cylindrical-shaped structure, and the length of bottle neck is set to 3.0-3.5 cm, a diameter of 3.6-
4.0 cm, the angle being loaded between the central axes of bottle neck 5 and horizontal plane is 40-60 °, is conducive to add in culture medium process for preparation each
Kind reagent, and after sterilizing sample absorption is carried out using utensils such as liquid-transfering guns in the small space of superclean bench so that add
Sample and take liquid operation more convenient, avoid liquid-transfering gun from touching on the inside of bottleneck and play cross contamination.As shown in figure 4, on sample-adding bottle neck 5
2 diameter of ventilating cover of configuration is set to 4.0-4.5 cm, and 2 capping of ventilating cover is equipped with the air hole 19 in concentric circles arrangement, breathes freely
The inner side of lid 2 is equipped with fungi-proofing filter membrane 20 and the annular silica gel packing ring 21 with radian, and fungi-proofing filter membrane 20 is replaceable, and passes through annular silica gel
The support of packing ring 21 is fixed, and 2 madial wall of ventilating cover is equipped with screw thread, and lateral wall is equipped with the anti-skidding raised line 22 of vertical bar shape.Fungi-proofing filter membrane 20
Be conducive to that the steam in high-temperature sterilization can circulate and bacterium cannot be by, reach fungi-proofing purpose, and after sterilizing inside bottle 1
Naturally it can rapidly be depressured, prevent culture medium from overflowing bottle 1, easy to sterile storage after sterilizing, preferably play fungi-proofing, anti-pollution mesh
, anti-skidding raised line 22 can increase frictional force when turning on ventilating cover 2, the opening operation of convenient bottle cap.
Preferably, described to topple over bottle neck 6 be cylindrical-shaped structure, and the length of bottle neck is set to 3.0-3.5 cm, a diameter of 3.0-
3.4 cm, the angle toppled between 6 central axes of bottle neck and horizontal plane is 40-70 °.The design at bottle neck angle of inclination meets ergonomic
Mechanics, has convenience that is easy to operate, being easy to pour plate and control flow.As shown in Fig. 5, topple over what is configured on bottle neck 6
3 diameter of sealing cover is set to 3.4-4.0 cm, and 3 inside top of sealing cover is equipped with the circular silica gel pad circle 23 with radian, in sealing cover 3
Side wall is equipped with screw thread, and lateral wall is equipped with the anti-skidding raised line 22 of vertical bar shape.
As shown in Fig. 6,1 outer wall of bottle is with dismountable collet 16 is arranged with, and collet 16 is preferably by heat-insulated, transparent
Silica gel material made of rectangular sheet structure, the vertical two sides of collet 16 are correspondingly provided with adhesive strip 25, two adhesive strip institutes
It is opposite in face（That is an adhesive strip 25 is in front, and another adhesive strip 25 is in reverse side）, collet 16 is coated on the outer wall of bottle 1
On, and fixation is mutually bonded by the adhesive strip 25 of both sides.In concrete operations, since 1 temperature of bottle after autoclaving is higher,
Single collet 16 cannot meet insulation requirement, therefore collet 16 can set multilayer, be coated on successively on the outer wall of bottle 1；
Certainly, single collet 16 can also be set it is thicker, to reach the heat insulation of higher, size and the bottle 1 of collet 16
Size is adapted, as long as can ensure that it is fitted tightly on 1 outer wall of bottle.
Preferably, 1 outer wall of bottle is equipped with graduation mark 17 and recording label 18, the minimum division value of graduation mark 17
For 50 mL, in agar medium stream process so that pour into that the amount of culture dish is accurate, and operation is quick, and then ensures that agar is put down
Nutrition uniformity between plate, the volume scope of bottle 1 are set to 500-800 mL.1 lower part of bottle is in rectangular parallelepiped structure, and length is set
For 9.0-10 cm, width is set to 6.5-7.5 cm, is highly set to 9-12 cm；Inside corresponding tilt at left and right sides of 1 top of bottle
In ramped shaped, the height on slope is set to 4.0-5.0 cm, and the angle between slope slope surface and horizontal plane is set to 30-50 °, in slope surface
Sample-adding bottle neck 5 is set respectively and topples over bottle neck 6.The thickness of bottle 1 is designed as taking during person's pour plate easy to operation, grasps bottle
With anti-slip in the middle part of body, bottle 1 occupies little space, and autoclaving processing can be carried out at the same time easy to multiple medium bottles；On bottle
The inclined plane in portion and the design of both sides tilting adding mouth and pour spout, meet ergonomic designs' requirement, easy to culture medium
Animal blood and various reagents are added or drawn in process for preparation, particularly in the small space of superclean bench, are being used
It is particularly suitable in longer measuring pipette or the sterile operation for being loaded, sampling of pipettor progress, take liquid or sample-adding operation more suitable
It is hand, convenient, can avoid liquid-transfering gun barral of a gun touch on the inside of bottleneck and caused by pollution；Secondly, it can effectively control and grasp pours into
The amount of culture medium in plate, is not likely to produce backflow phenomenon, conveniently pours into, and improves the accuracy rate and success rate poured into, time saving, high
Effect.1 bottom indent of bottle, is designed with antiskid thread 24 bottom outer-edge week, and indent design can avoid the attachment of bottle 1 from being adhered to work
Table top, the fluency that increase bottle 1 moves, screw thread 24 can increase the stability of placement, avoid slipping desktop, it is ensured that safety.Institute
State bottle 1 to be made of Pyrex, plug 4 main body of core uses polypropylene（PP）Or makrolon（PC）It is made, the bottle cap（Thoroughly
Gas lid 2 and sealing cover 3）It is made of phenolic resin, above-mentioned material can meet the examination of high pressure steam sterilization and Reusability
Test requirement, bottle 1 and plug core 4 to be made of the high material of transparency, be conducive to the shape that operating personnel observe culture medium in bottle 1
State, easy to observe, control the volume of addition/absorption culture medium and reagent, facilitates pouring operation and controls the culture of pour plate
Dryweather flow.
Specifically, culture medium bottle dimensional structure of the invention is specific as follows, bottle lower part cuboid length × width × height point
Not Wei the cm of the cm of 10 cm × 7.0 × 9.0, the height of upper end ramp structure is 4.5 cm, and the angle of slope slope surface and horizontal plane is
40 °, 1 volume of bottle is 600 mL, and the length of sample-adding bottle neck 5 is 3.3 cm, and opening diameter is 3.8 cm, is loaded in bottle neck 5
Angle between axis and horizontal plane is 50 °, and it is 3.3 cm to topple over 6 length of bottle neck, and opening diameter is 3.2 cm, topples over bottle neck 6
Angle between central axes and horizontal plane is 50 °, and the length of plug core 4 is 7.3 cm, and inner hollow diameter is 2.8 cm, and wherein upper end is poured into
9 length of mouth are 2.3 cm, and 10 length of middle-end protrusion and outside diameter are respectively 1.8 cm and 4.0 cm, and 11 length of lower port is 3.2
Cm, a diameter of 4.2 cm of ventilating cover 2, is highly 3.0 cm, and a diameter of 3.6 cm of sealing cover 3, is highly 2.4 cm.
The present invention is carried out when carrying out blood agar culture-medium preparation essentially according to following steps：
According to requirement of experiment, required dehydrated medium, reagent and ormal weight distilled water are added into bottle by being loaded bottle neck 5
Interior, preliminarily solubilised mixes, and the plug insertion of core 4 is toppled over bottle neck 6, screwing hermetic lid 3 and ventilating cover 2, carry out high steam and go out respectively
Bacterium.In sterilization process, high-temperature steam can reach sterilization effect by air hole 19 into bottle；Sterilize the later stage, high pressure is steamed
Gradual pressure release in vapour autoclave, is declined, the culture in bottle at the same time by air pressure in 19 bottle of air hole with ambient pressure
Base will not because in bottle air pressure it is excessive and overflow.Fungi-proofing filter membrane 20 can also make the liquid after sterilizing keep germ-free condition, reach nothing
The purpose of bacterium storage.Sterilizing terminates, and vapour more than exclusion, pressure release to zero, bottle is taken out, is put into superclean bench, treats bottle temperature
Degree is down in the range of 50 ~ 55 DEG C, and collet 16 of becoming reconciled is glued around bottle（Different layers can be bonded according to the temperature of culture medium in bottle
Several collets）.Ventilating cover 2 is turned on, the aseptic de-fiber sheep blood of 5% ~ 10% (V/V) of sterile addition, is tightened in culture medium
Gas lid 2, slowly rotates bottle, mixes culture medium and de- fiber sheep blood manually.After mixing unanimously, sealing-plug 3 is unscrewed, into promoting circulation of blood
Agar medium is poured into, dispensed, and is filled in double cambered surface strainers 12 in core 4, can be effectively filtered out liquid blood culture base table in bottle
Face is because of a large amount of foams caused by quick shake up, it is ensured that smooth, even curface is formed after the blood agar culture-medium solidification poured into,
Avoid standing or the heating efficiency that defoams is low, skewness, the phenomenon of solidification easily occurs in culture medium, it is ensured that blood agar culture
Improved work efficiency while quality.
The present invention can filter out in bottle liquid blood media surface because rotation mix caused by a large amount of foams, while can
It is further ensured that culture medium and blood reach optimal mixing state, foam removes quick, efficient, it is ensured that the blood agar culture poured into
Smooth, smooth consistent surface is formed after base solidification.Poured into due to carrying out a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices within the temperature range of regulation, and pour plate
Amount it is quick, accurate, thus avoid the agar in bottle and de- fiber sheep blood produce the phenomenon of skewness, between agar plate
Nutritional ingredient easily reaches uniform unified standard, improves the accuracy rate and success rate poured into, and is the standard of follow-up test result
True property provides technical guarantee.Secondly, its bottleneck size, angle of inclination and bottle cap design are reasonable, facilitate fluid nutrient medium
Autoclave sterilization disinfection, easy to carry out sterile sample-adding or imbibition operation in superclean bench, conveniently pours into, avoids back
The generation of flow phenomenon, it is time saving, efficient, the configuration and packing of bacterial solids, semisolid culturemedium can be preferably completed, so as to subtract
Gently, the workload of lab assistant is simplified.
Above example is only exemplary, and can't limit to the present invention, it should be pointed out that for those skilled in the art
For, the other equivalent modifications made under technical inspiration provided by the present invention and improvement, are regarded as guarantor of the invention
Protect scope.
Claims (9)
1. a kind of medium bottle for being used to prepare blood agar plate, including bottle, bottle outer wall is equipped with graduation mark, its feature exists
In：The bottle（1）Upper end is equipped with respectively the inclined sample-adding bottle neck in both sides to the left and right（5）With topple over bottle neck（6）, the dumping bottle
Mouth（6）Equipped with built-in plug core（4）And sealing cover（3）, wherein filling in core（4）With toppling over bottle neck（6）Inner wall realizes that seamless seal inserts,
Fill in core（4）Lower end is equipped with double-deck, convex cambered surface strainer（12）, fill in core（4）Upper end passes through screw thread and sealing cover（3）Screw solid
It is fixed；The sample-adding bottle neck（5）It is configured with capping and is evenly distributed with air hole（19）Ventilating cover（2）, ventilating cover（2）With being loaded bottle neck（5）
It is tightened by screw thread；
The plug core（4）For hollow columnar structures, by upper end pouring opening（9）, middle-end protrusion（10）And lower port（11）Three
Part composition integral structure；Wherein, lower port（11）Inner side is equipped with the strainer being fixed with one with it（12）, lower port（11）
Outer wall is equipped with silica gel spiral mouth（13）, fill in core（4）Pass through silica gel spiral mouth（13）With toppling over bottle neck（6）Inner wall realizes that sealing is inserted
Dress；The middle-end protrusion（10）Outside diameter be more than topple over bottle neck（6）Bore, and its lower face and topple over bottle neck（6）Bottle
Mouth edge is in close contact；The upper end pouring opening（9）Outer wall is equipped with plug core screw thread（14）, itself and sealing cover（3）Revolved by screw thread
Fasten.
2. the medium bottle according to claim 1 for being used to prepare blood agar plate, it is characterised in that：The bottle（1）
Outer wall, which is matched somebody with somebody, is arranged with dismountable collet（16）.
3. the medium bottle according to claim 2 for being used to prepare blood agar plate, it is characterised in that：The collet
（16）For rectangular sheet structure made of silica gel, collet（16）Vertical two sides be correspondingly provided with adhesive strip（25）, by viscous
Narrow bars（25）By collet（16）Wrap in bottle（1）Outer wall.
4. the medium bottle according to claim 1 or 2 for being used to prepare blood agar plate, it is characterised in that：It is described ventilative
Lid（2）Capping is equipped with the air hole in concentric circles arrangement（19）, ventilating cover（2）Inner side is equipped with fungi-proofing filter membrane（20）With band radian
Annular silica gel packing ring（21）, fungi-proofing filter membrane（20）Pass through annular silica gel packing ring（21）Support is fixed, ventilating cover（2）Madial wall is set
There is screw thread, lateral wall is equipped with the anti-skidding raised line of vertical bar shape（22）.
5. the medium bottle according to claim 1 or 2 for being used to prepare blood agar plate, it is characterised in that：The sealing
Lid（3）Inside top is equipped with the circular silica gel pad circle with radian（23）, sealing cover（3）Madial wall is equipped with screw thread, and lateral wall is equipped with
The anti-skidding raised line of vertical bar shape（22）.
6. the medium bottle according to claim 1 or 2 for being used to prepare blood agar plate, it is characterised in that：The sample-adding
Bottle neck（5）Central axes and horizontal plane between angle be 40-60 °, topple over bottle neck（6）Central axes and horizontal plane between angle be
40-70°。
7. the medium bottle according to claim 1 for being used to prepare blood agar plate, it is characterised in that：The bottle（1）
Lower part is in rectangular parallelepiped structure, and inwardly corresponding tilt is in ramped shaped for the top left and right sides, and sample-adding bottle neck is set respectively in the slope surface of slope
（5）With topple over bottle neck（6）.
8. the medium bottle according to claim 7 for being used to prepare blood agar plate, it is characterised in that：The bottle（1）
Angle between slope slope surface and horizontal plane is set to 30-50 °.
9. the medium bottle according to claim 1 for being used to prepare blood agar plate, it is characterised in that：The bottle（1）
It is made of Pyrex, fills in core（4）Main body is made of polypropylene or makrolon, sealing cover（3）And ventilating cover（2）Adopt
It is made of phenolic resin.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610677019.9A CN106047675B (en) | 2016-08-17 | 2016-08-17 | A kind of medium bottle for being used to prepare blood agar plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610677019.9A CN106047675B (en) | 2016-08-17 | 2016-08-17 | A kind of medium bottle for being used to prepare blood agar plate |
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| Publication Number | Publication Date |
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| CN106047675A CN106047675A (en) | 2016-10-26 |
| CN106047675B true CN106047675B (en) | 2018-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610677019.9A Active CN106047675B (en) | 2016-08-17 | 2016-08-17 | A kind of medium bottle for being used to prepare blood agar plate |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109337795A (en) * | 2018-11-15 | 2019-02-15 | 张树军 | Automatic pressure-release container |
| CN109580938A (en) * | 2019-01-11 | 2019-04-05 | 广州万孚生物技术股份有限公司 | Loading bottom plate and immuno-chromatography detection device containing the loading bottom plate |
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| CN202379985U (en) * | 2011-12-26 | 2012-08-15 | 王成军 | Bacteria culture flask for multiple sampling |
| CN202966991U (en) * | 2012-09-28 | 2013-06-05 | 陈水谦 | T-shaped bottle stopper |
| CN204473486U (en) * | 2015-03-11 | 2015-07-15 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of liquid autoclave sterilization bottle |
| CN204939472U (en) * | 2015-09-16 | 2016-01-06 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of nutrient agar prepares bottle |
| CN205893261U (en) * | 2016-08-17 | 2017-01-18 | 中国农业科学院兰州畜牧与兽药研究所 | A culture medium bottle for preparing blood agar plate |
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2016
- 2016-08-17 CN CN201610677019.9A patent/CN106047675B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202379985U (en) * | 2011-12-26 | 2012-08-15 | 王成军 | Bacteria culture flask for multiple sampling |
| CN202966991U (en) * | 2012-09-28 | 2013-06-05 | 陈水谦 | T-shaped bottle stopper |
| CN204473486U (en) * | 2015-03-11 | 2015-07-15 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of liquid autoclave sterilization bottle |
| CN204939472U (en) * | 2015-09-16 | 2016-01-06 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of nutrient agar prepares bottle |
| CN205893261U (en) * | 2016-08-17 | 2017-01-18 | 中国农业科学院兰州畜牧与兽药研究所 | A culture medium bottle for preparing blood agar plate |
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