CN207193279U - A kind of culture transferring bottle - Google Patents
A kind of culture transferring bottle Download PDFInfo
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- CN207193279U CN207193279U CN201720691054.6U CN201720691054U CN207193279U CN 207193279 U CN207193279 U CN 207193279U CN 201720691054 U CN201720691054 U CN 201720691054U CN 207193279 U CN207193279 U CN 207193279U
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- bottle
- culture transferring
- liquid level
- culture
- bottle cap
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Abstract
The utility model discloses a kind of culture transferring bottle, it is related to microorganism seed fermentation technical field, including bottle, bottle cap, liquid level gauge, culture transferring valve, culture transferring pipe, culture transferring head, sterilization tray, the bottle is provided with bottleneck, the bottleneck is threadedly coupled with bottle cap, bottle cap bottom surface is provided with seal washer, bottle cap side is provided with steam vent, steam vent is located at below seal washer, the bottle is provided with liquid level gauge, liquid level gauge both ends connect with bottle top and bottle bottom respectively, bottle bottom connects with culture transferring valve one end, the culture transferring valve other end connects with culture transferring pipe one end, the culture transferring pipe other end connects with the first end of culture transferring, the liquid level is calculated as high temperature resistant liquid level meter, liquid level is calculated as transparent material, liquid level gauge is provided with scale.The utility model is not only simple in construction, simple operation, has the function of aseptic inoculation, also has the function that accurately determine culture transferring amount.
Description
Technical field
It the utility model is related to microorganism seed fermentation technical field, more particularly to a kind of culture transferring bottle.
Background technology
During existing fermentation culture transferring, inoculation bottle is typically just for one grade fermemtation field, i.e. by ready seed liquor by
Inoculation bottle enters in the person's of being transferred to fermentation tank, but, it is necessary to strain culture transferring will be produced extremely in the production process of multistage fermentation and experiment
In advanced seeding tank or experiment round, kind and culture transferring is entirely taken to cross range request and complete under aseptic conditions.Do it is related with
When track is tested, existing culture transferring technology be typically by the way of pipeline culture transferring or taken using culture transferring bottle in kind backward round
Strain is poured into, the most frequently used culture transferring bottle is glass culture transferring bottle and pressure difference bottle, but existing pipeline culture transferring mode will to space enrironment
Ask strict, it is limited that experiment round apart from seed liquor storeroom may result in pipeline inoculation too far, the defects of glass culture transferring bottle
That it is easily broken and be inconvenient to transport, pressure difference bottle be by the pressure differential progress culture transferring between round and inoculation bottle,
But kind of an inconvenience is taken, culture transferring amount not can determine that, and pressure adjustment improper can cause determine whether kind of liquid injects hair during culture transferring
In ferment container or it may cause, with microbiological contamination risk, not only to there is danger, also reduce practicality.
Utility model content
The utility model aims to provide a kind of culture transferring bottle, takes kind of an inconvenience for solving existing culture transferring bottle, can not determine culture transferring
Measure, be easily caused the problem of kind of liquid microbiological contamination.
To reach above-mentioned purpose, the technical solution adopted in the utility model is as follows：A kind of culture transferring bottle, including bottle, it is described
Bottle is provided with bottleneck, in addition to bottle cap, liquid level gauge, culture transferring valve, culture transferring pipe, culture transferring head, the bottleneck are threadedly coupled with bottle cap,
Bottle cap bottom surface is provided with seal washer, and bottle cap side is provided with steam vent, and steam vent is located at below seal washer, and the bottle is provided with liquid
Position meter, liquid level gauge both ends connect with bottle top and bottle bottom respectively, and bottle bottom connects with culture transferring valve one end, culture transferring valve
The door other end connects with culture transferring pipe one end, and the culture transferring pipe other end connects with the first end of culture transferring.
Preferably, the liquid level gauge is vertically arranged.
Preferably, the liquid level is calculated as high temperature resistant liquid level meter, and liquid level is calculated as transparent material, and liquid level gauge is provided with scale, described
Scale is located at outside bottle.
Preferably, the seal washer is silica gel material, and seal washer is adapted with bottle cap bottom surface.
Preferably, during bottle cap screwing, the steam vent is located at the lower edge of seal washer.
Further, the culture transferring bottle also includes sterilization tray, and the sterilization tray is annularly set in bottle top.
Preferably, the culture transferring bottle is volume 2-8L pressure-resistant stainless steel cylinder.
Preferably, the culture transferring valve is diaphragm valve.
Preferably, the culture transferring pipe is wriggling pump hose, and the wriggling pump hose is used cooperatively in culture transferring with peristaltic pump.
Preferably, the culture transferring head is L-type high temperature high voltage resistant stainless steel tube.
Preferably, the Bottle cap top is provided with rotating handle.
Further, the rotating handle includes a vertical rod and a cross bar, and described vertical rod one end connects Bottle cap top, and vertical rod is another
In the middle part of one end connection cross bar.
Preferably, the culture transferring bottle also includes handle, and handle has two.
Further, the bottle outer wall that two handles are symmetrically located at below the sterilization tray.
The utility model is simple in construction, it is easy to operate, be readily transported, the setting of liquid level gauge can be clearly seen that inoculation and
Culture transferring amount, the setting of sterilization tray can be such that the utility model is used under the environment that carries disease germs, make the utility model not by using field
The limitation on ground, sterilizing is thorough, safe and convenient to use.
Brief description of the drawings
Fig. 1 is the utility model structure diagram.
In figure：1- bottle caps, 2- sterilization tray, 3- handles, 4- bottles, 5- liquid level gauges, 6- culture transferrings valve, 7- culture transferrings pipe, 8-
Culture transferring head, 9- seal washers, 10- rotating handles, 11- steam vents.
Embodiment
In order that the purpose of this utility model, technical scheme and advantage are more clearly understood, below in conjunction with accompanying drawing, to this reality
It is further elaborated with new.
As shown in figure 1, a kind of culture transferring bottle, including bottle 4, the bottle 4 have the bottleneck of the taper positioned at the top of bottle 4
With the bottleneck positioned at the top of bottle 4, in addition to bottle cap 1, liquid level gauge 5, culture transferring valve 6, culture transferring pipe 7, culture transferring first 8, the bottleneck
It is threadedly coupled with bottle cap 1, the bottom surface of bottle cap 1 is provided with the seal washer 9 of silica gel material, and seal washer 9 is adapted with the bottom surface of bottle cap 1,
Ensure that bottleneck can be fully sealed, the side of bottle cap 1 is provided with steam vent 11, and steam vent 11 is located at the lower section of seal washer 9, and bottle cap 1 revolves
Next, steam vent 11 is located at the lower edge of seal washer 9, conversely, after bottle cap 1 and bottleneck unclamp, the part of steam vent 11 is high
Bottle outlet, now the gas in bottle 4 can be discharged by steam vent 11, the liquid level gauge 5 is vertically on the bottle 4, liquid
Position 5 both ends of meter connect with the upper and lower part of bottle 4 respectively, and liquid level gauge 5 is transparent material, in order to observe strain amount in bottle 4,
Liquid level gauge 5 also has scale, and the scale is located at outside bottle, in order to accurately read strain variable quantity in bottle 4, liquid level gauge 5
Also there is resistant to elevated temperatures characteristic, the bottom of bottle 4 connects with the one end of culture transferring valve 6, and culture transferring valve 6 is barrier film valve, culture transferring valve 6
The other end connects with the one end of culture transferring pipe 7, and culture transferring pipe 7 is wriggling pump hose 7, and the other end of culture transferring pipe 7 connects with first 8 one end of culture transferring,
Culture transferring first 8 is L-type high temperature high voltage resistant stainless steel tube.
The culture transferring bottle also includes sterilization tray 2 annular in shape, and sterilization tray 2 is sleeved on outside the bottleneck of bottle 4, sterilizing support
Disk 2 is used to, in inoculation, place combustible on sterilization tray 2, form pyrosphere after lighting, all kinds of in air when eliminating inoculation
Bacterium, bacterium solution is prevented to be contaminated.
The culture transferring bottle also includes a pair of handles 3, and two handles 3 are symmetrically disposed on the outer wall of bottle 4, two handles 3
In the lower section of sterilization tray 2, in order to which user shifts to culture transferring bottle.
When in use, culture transferring first 8 is wrapped up with 8 layers of gauze first, and is restricted with silk floss and fasten 8 layers of gauze, then outside 8 layers of gauze
One layer of brown paper of parcel is simultaneously fastened with cotton cord, is then opened culture transferring valve 6, is unscrewed bottle cap 1, makes 11 high bottle outlet of steam vent, make
Bottle 4 is in communication with the outside, and culture transferring bottle is placed in sterilizing chamber and sterilized.After the completion of sterilizing, culture transferring valve 6 is closed, is turned on
Bottle cap 1, strain liquid is added into bottle 4 in sterilizing chamber, certainly, the process of inoculation can also occur outside sterilizing chamber, now
Need first to light a circle pyrosphere on sterilization tray 2, bottle cap 1 is then opened by rotating handle 10, can be had by rotating handle 10
Effect avoids injury of the flame to human body, then strain liquid is added into bottle 4, completes inoculation.This two kinds of different vaccination ways, all
Inoculum concentration can be observed by liquid level gauge 5.After the completion of inoculation, bottle cap 1 is tightened, remove comburant, by handle 3 by culture transferring bottle
Moving to is needed at the seeding tank or experimental tank of culture transferring, and culture transferring first 8 is connected into seeding tank or experimental tank, then by wriggling
Pump, culture transferring pipe 7 is connected with peristaltic pump, open peristaltic pump and culture transferring valve 6, in the presence of peristaltic pump, the bacterium in bottle 4
Kind liquid needs the seeding tank or experimental tank of culture transferring, finally, passes through sight by culture transferring valve 6, culture transferring pipe 7, culture transferring first 8, flow direction
The scale of liquid level gauge 5 is examined, determines whether strain liquid enters seeding tank or experimental tank, can more determine culture transferring amount.
Certainly, the utility model can also have other various embodiments, without departing substantially from the utility model spirit and its essence
In the case of, those skilled in the art can make various corresponding changes and deformation, but these phases according to the utility model
The change and deformation answered should all belong to the scope of the claims appended by the utility model.
Claims (10)
1. a kind of culture transferring bottle, including bottle, the bottle is provided with bottleneck, it is characterised in that：Also include bottle cap, liquid level gauge, culture transferring
Valve, culture transferring pipe, culture transferring head, the bottleneck are threadedly coupled with bottle cap, and bottle cap bottom surface is provided with seal washer, and bottle cap side is provided with row
Stomata, steam vent are located at below seal washer, and the bottle is provided with liquid level gauge, liquid level gauge both ends respectively with bottle top and bottle
Bottom connects, and bottle bottom connects with culture transferring valve one end, and the culture transferring valve other end connects with culture transferring pipe one end, and culture transferring pipe is another
End connects with the first end of culture transferring.
A kind of 2. culture transferring bottle according to claim 1, it is characterised in that：The seal washer is silica gel material, sealing gasket
Circle is adapted with bottle cap bottom surface.
A kind of 3. culture transferring bottle according to claim 1, it is characterised in that：During bottle cap screwing, the steam vent is positioned at sealing
The lower edge of packing ring.
A kind of 4. culture transferring bottle according to claim 1, it is characterised in that：Also include sterilization tray, the sterilization tray is in
Annular casing is loaded on bottle top.
A kind of 5. culture transferring bottle according to claim 1, it is characterised in that：The culture transferring bottle is the pressure-resistant stainless of volume 2-8L
Steel cylinder.
A kind of 6. culture transferring bottle according to claim 1, it is characterised in that：The culture transferring valve is diaphragm valve.
A kind of 7. culture transferring bottle according to claim 1, it is characterised in that：The culture transferring pipe is wriggling pump hose, the shifting
Kind head is L-type high temperature high voltage resistant stainless steel tube.
A kind of 8. culture transferring bottle according to claim 1, it is characterised in that：The liquid level is calculated as high temperature resistant liquid level meter, liquid level
Transparent material is calculated as, liquid level gauge is provided with scale, and the scale is located at outside bottle.
A kind of 9. culture transferring bottle according to claim 1, it is characterised in that：The Bottle cap top is provided with rotating handle, the rotation
Turning handle includes a vertical rod and a cross bar, and described vertical rod one end connects Bottle cap top, vertical rod other end connection cross bar middle part.
A kind of 10. culture transferring bottle according to claim 4, it is characterised in that：Also including handle, handle has two, and two one or two
The bottle outer wall that hand is symmetrically located at below the sterilization tray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720691054.6U CN207193279U (en) | 2017-06-14 | 2017-06-14 | A kind of culture transferring bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720691054.6U CN207193279U (en) | 2017-06-14 | 2017-06-14 | A kind of culture transferring bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207193279U true CN207193279U (en) | 2018-04-06 |
Family
ID=61784909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720691054.6U Active CN207193279U (en) | 2017-06-14 | 2017-06-14 | A kind of culture transferring bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207193279U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384708A (en) * | 2018-05-23 | 2018-08-10 | 宁夏西麒麟生物科技有限公司 | A kind of sterile controlled amounts type classification inoculation apparatus of seeding tank and the sterile controlled amounts type inoculation method for fermenting or cultivating |
-
2017
- 2017-06-14 CN CN201720691054.6U patent/CN207193279U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384708A (en) * | 2018-05-23 | 2018-08-10 | 宁夏西麒麟生物科技有限公司 | A kind of sterile controlled amounts type classification inoculation apparatus of seeding tank and the sterile controlled amounts type inoculation method for fermenting or cultivating |
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