CN205893261U - A culture medium bottle for preparing blood agar plate - Google Patents

Abstract

The utility model discloses a culture medium bottle for preparing blood agar plate, including the bottle, the bottle outer wall is equipped with the scale mark, bottle upper end be equipped with respectively to the application of sample bottle neck of left and right sides slope with topple over the bottle neck, empting the bottle neck and being equipped with built -in stopper core and sealed lid, wherein fill in the core and realize seamless sealed cartridge with empting the bottle neck inner wall, stopper core lower extreme is equipped with the cambered surface filter screen of bilayer, epirelief, and stopper core upper end is screwed fixedly through screw thread and sealed lid, the application of sample bottle neck disposes the ventilating cover of capping equipartition bleeder vent, and ventilating cover screws fixedly with the application of sample bottle neck through the screw thread. Through the stopper core that has double -deck filter screen, effectively base surface is cultivateed because of shaking even produced a large amount of foams fast to liquid blood in the filtering bottle for the blood nutrient agar who pours into solidifies the back and forms smooth, level and smooth, unanimous surface, has improved operating efficiency when guaranteeing blood nutrient agar quality, bottle structure reasonable in design, and be convenient for experiment operation and experiment parameter control can popularize and apply.

Description

A kind of medium bottle for preparing blood agar plate

Technical field

This utility model belongs to Experiment on Microbiology fixture technology field, is related to a kind of medium bottle, refers specifically to one kind and is used for Prepare the medium bottle of blood agar plate.

Background technology

Blood agar culture-medium is a kind of beef extract-peptone training containing de- fiber animal blood (typically using Sanguis Leporis seu oryctolagi or Sanguis caprae seu ovis) Foster base, moreover it is possible to provide the spies such as coenzyme (as the v factor), haemachrome (the x factor) in addition to containing the various nutrition required for culture antibacterial Different somatomedin, is usually used in cultivating, separate and preserve some pathogenic microorganisms harsh to nutritional requirement in microbiological test； In addition, it may also be used for measure the haemolysiss of antibacterial.In view of the rigors that some antibacterials are prepared to blood agar culture-medium, such as train Need to be incubated at 45 DEG C about after foster base sterilizing, proportionally add aseptic de- fiber Sheep Blood, after fully mixing, pour into sterilizing training Foster ware.If temperature is too high, the blood plate color prepared can black, and certainly will affect result of the test or be difficult to observe antibacterial Haemolysis circle, therefore must be cooled to and temperature required could add Sanguis caprae seu ovis.Because business blood agar culture-medium expense is prohibitively expensive, And used medium haves such problems as that preparation overlong time, shelf-life are limited, tend not to meet laboratory standard requirement.For Guarantee the time effects that experimental result is not prepared by agar plate, save experimentation cost, for this reason, laboratory often needs manually to incline in a large number Fall to make blood agar culture-medium by oneself.At present, the preparation of blood agar culture-medium generally individually adopts glass spinner flask, needs first by the training preparing Foster based sols are poured in conical flask, 121 DEG C of autoclavings 15 minutes, are cooled to 50 ~ 55 DEG C, and sterile working adds 5% ~ 10% (v/ V) aseptic de-fiber sheep blood of pre-temperature to 37 DEG C, fully mixes, pours into sterilized petri dishes, you can.But during test operation often There is problems in that (1), during fully mixing culture medium and de- fiber Sanguis caprae seu ovis, often can produce substantial amounts of floatation of foam On the liquid level mixing culture medium, defoamed usually through standing or heating in the past, but be as prolongation and the training of operating time The decline of foster base temperature, easily skewness in medium component, or even the phenomenon solidifying, and mixes again, can produce again A large amount of foams, foam clearance is relatively low；When pouring into sterilized petri dishes, float on liquid level foam be poured into culture medium flat In ware, after culture medium solidifying, its surface often forms more pit (lather collapse causes) not of uniform size it is impossible to prepare Smooth, smooth media surface needed for test requirements document, in later scribes inoculation, painting work, often occurs scratching agar The phenomenon on surface, the accuracy of impact result of the test.(2) during pouring operation, usually left hand is responsible for taking sterilizing and is put down Ware, the right hand is held bottle and is constantly mixed operation, to be further ensured that culture medium and blood reach optimal mixing state.Due to agar culture Need when base pours into keep 50 DEG C about of temperature, in a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices stream process, the amount of pour plate needs quickly, accurately, if inclining Note overlong time, then with the reduction of temperature, the agar in conical flask and de- fiber Sanguis caprae seu ovis will produce the phenomenon of skewness, lead Cause nutritional labeling between agar plate uneven it is impossible to meet the uniformity of experiment condition, widen the difference of experimental result.(3) current Carry out the sterilization of culture medium using conical flask plus silica gel plug and tinfoil sealing, there is sealing easy gas leakage rupture etc. after sterilization and ask Topic.And using common tool plug reagent bottle, due to the no ventilative design of bottleneck, need to unclamp bottleneck in high-pressure sterilizing course and sterilized Sterilization, easily causes the hidden danger such as culture medium spilling, the built-in storehouse of pollution high voltage steam sterilization pan and water trapping layer.In addition, common tool plug The bottleneck size of reagent bottle, angle of inclination and bottle cap different design close ergonomic designs and require, and exist in operation and use The problem of inconvenience.(4) traditional glass conical flask body there is a problem of being difficult grasping, in addition conical flask after autoclaving, from When pressure cooker takes out, its body temperature is excessively boiling hot, increased grasping difficulty, easily scalds handss, not easy to operate, and increase is poured into, subpackage blood The time of flat board；And bottle temperature need to control in the range of 50 ~ 55 DEG C when pouring into, because operation process is slow, often make taper In bottle, the culture medium that is not finished down is close to solidification, need to reheat, melt after pour into blood plate again, thus, increased culture Base pollution probability, lead to the plating medium temperature poured into inconsistent, easily cause culture medium result of the test repeat parallel Diversity is larger.(5) if the culture base unit weight prepared in bottle is excessive, in originally pour plate, wayward pour each into The amount of culture dish, topples over bottleneck during culture medium and is also easy to produce backflow, easily cause pollution, operation is wasted time and energy, and the blood plate of preparation is difficult Required with reaching unified standard, requirement can not be met in the experiment of culture of comparing, at present this be there is no with preferable solution Method.

Utility model content

For above-mentioned technical problem, this utility model provides a kind of medium bottle for preparing blood agar plate, energy Rapidly and efficiently remove a large amount of foams mixing culture medium liquid level it is ensured that improve operation effect while blood agar culture-medium quality Rate, Bottle structure is reasonable in design, is easy to experimental implementation and experiment parameter controls.

This utility model is achieved through the following technical solutions above-mentioned purpose:

A kind of medium bottle for preparing blood agar plate, including bottle, bottle outer wall is provided with graduation mark, described bottle Upper end is provided with respectively the sample-adding bottle neck that both sides to the left and right tilt and topples over bottle neck, described topple over bottle neck and is provided with built-in plug core and sealing Lid, wherein plug core realize seamless seal plug-in mounting with toppling over bottle neck inwall, and plug core lower end is provided with double-deck, convex cambered surface filter screen, plug Core upper end is tightened with closure by screw thread；Described sample-adding bottle neck is configured with the ventilating cover of the uniform air-vent of capping, breathes freely Lid is tightened by screw thread with sample-adding bottle neck.

As the prioritization scheme of this case, described plug core is hollow columnar structures, by upper end pouring opening, middle-end protuberance and Lower port three part forms integral structure；Wherein, it is provided with the filter screen being fixed with one with it, lower port outer wall inside lower port It is provided with silica gel spiral mouth, plug core passes through silica gel spiral mouth, and realization seals plug-in mounting with toppling over bottle neck inwall；Described middle-end protuberance External diameter is more than the bore toppling over bottle neck, and its lower surface is in close contact with the bottleneck edge toppling over bottle neck；Described upper end pouring opening Outer wall is provided with plug core screw thread, and it is tightened by screw thread with closure.

As the prioritization scheme of this case, described bottle outer wall is supporting to be provided with dismountable collet.

The rectangular sheet structure made for silica gel as the prioritization scheme of this case, described collet, vertical the two of collet Side is correspondingly provided with adhesive strip, is wrapped collet in bottle outer wall by adhesive strip.

As the prioritization scheme of this case, described ventilating cover capping is provided with the air-vent of the arrangement in concentric circles, in ventilating cover Side is provided with fungi-proofing filter membrane and the annular silicone gasket with radian, and fungi-proofing filter membrane passes through annular silicone gasket and supports fixation, ventilating cover Medial wall is provided with screw thread, and lateral wall is provided with the anti-skidding raised line of vertical bar shape.

As the prioritization scheme of this case, described closure inside top is provided with the circular silica gel pad circle with radian, closure Medial wall is provided with screw thread, and lateral wall is provided with the anti-skidding raised line of vertical bar shape.

As the prioritization scheme of this case, the angle between the axis of described sample-adding bottle neck and horizontal plane is 40-60 °, topples over Angle between the axis of bottle neck and horizontal plane is 40-70 °.

As the prioritization scheme of this case, described bottle bottom is in rectangular structure, the inside corresponding tilt in the top left and right sides In ramped shaped, it is respectively provided with sample-adding bottle neck on slope is domatic and topples over bottle neck.

As the prioritization scheme of this case, the domatic angle and between horizontal plane in described bottle slope is set to 30-50 °.

As the prioritization scheme of this case, described bottle is made using Pyrex, and plug core main body adopts polypropylene or poly- carbon Acid esters is made, and closure and ventilating cover are all made using phenolic resin.

The beneficial effects of the utility model are:

(1) by being provided with the plug core of double-layer leaching net structure, liquid blood media surface in bottle can be effectively filtered out Because quickly shaking up produced a large amount of foams it is ensured that forming smooth, smooth, consistent table after the blood agar culture-medium solidification poured into Face；The three stage structure design of plug core, good airproof performance, the tight not water clock of laminating；

(2) design of the inclined plane on bottle top and both sides tilting adding mouth and pour spout, reasonable in design, meet Ergonomic designs require, and are easy in culture medium process for preparation add or draw various reagents so that taking liquid or sample-adding operation More smoothly, convenient, it is to avoid between vessel, touching pollutes；And effective control and grasp pour into the culture medium in plate Amount, is not likely to produce backflow phenomenon, conveniently pours into, and improves the accuracy rate poured into and success rate；

(3) bottle outer wall setting collet, is on the one hand easy to operator and takes, grasps the higher bottle of temperature, it is to avoid Scald；On the other hand play the effect of insulation, extend the time that medium bottle maintains in optimum temperature range, beneficial to mixed Even, dumping bottle culturing in vivo base and the operation of Sanguis caprae seu ovis, for pouring into, subpackage has striven for the time so that culture medium during pour plate Temperature is consistent, and improves the success rate of culture medium preparation；

(4) in ventilating cover air-vent and fungi-proofing filter membrane design so that during high-temperature sterilization steam can circulate and antibacterial not Can, reach aseptic storage purpose, and internal natural, the rapid blood pressure lowering of bottle after sterilizing, can be made, prevent culture medium from overflowing bottleneck；

(5) closure top inner side setting sealing silicone gasket, closure is connected by thread seal with plug core, further Guarantee that in high-pressure sterilizing course, culture medium will not overflow bottleneck；

(6) rational in infrastructure, simple operation, there is preferable market prospect and economic benefit, be suitable to popularization and application.

Brief description

Fig. 1 prepares the structural representation of blood agar plate medium bottle for this utility model；

Fig. 2 prepares the overall structure diagram of blood agar plate medium bottle for this utility model；

Fig. 3 prepares the structural representation filling in core in blood agar plate medium bottle for this utility model；

Fig. 4 prepares the top view of ventilating cover in blood agar plate medium bottle for this utility model；

Fig. 5 prepares the top view of closure in blood agar plate medium bottle for this utility model；

Fig. 6 prepares the structural representation of collet in blood agar plate medium bottle for this utility model；

In figure: 1- bottle, 2- ventilating cover, 3- closure, 4- fills in core, and 5- is loaded bottle neck, and 6- topples over bottle neck, 7- bottle neck spiral shell Stricture of vagina, 8- bottle cap female thread, 9- upper end pouring opening, 10- middle-end protuberance, 11- lower port, 12- filter screen, 13- silica gel spiral mouth, 14- fills in core screw thread, 15- female thread, 16- collet, 17- graduation mark, 18- label, 19- air-vent, the fungi-proofing filter membrane of 20-, 21- ring Shape silicone gasket, the anti-skidding raised line of 22-, 23- circular silica gel pad circle, 24- antiskid thread, 25- adhesive strip.

Specific embodiment

Below in conjunction with drawings and Examples, this utility model and its effect are further elaborated.

As shown in Figure 1, 2, a kind of medium bottle for preparing blood agar plate, including bottle 1, described bottle 1 upper end It is provided with the sample-adding bottle neck 5 that both sides tilt to the left and right respectively and topples over bottle neck 6, topple over bottle neck 6 and be provided with supporting built-in plug core 4 and close Capping 3；Wherein plug core 4 realizes seamless seal plug-in mounting with toppling over bottle neck 6 inwall, and plug core 4 lower end is provided with double-deck, convex cambered surface filter Net 12, plug core 4 upper end is tightened with closure 3 by screw thread；Sample-adding bottle neck 5 is configured with the ventilative of the uniform air-vent of capping 19 Lid 2, sample-adding bottle neck 5 upper end outer wall is provided with bottle neck screw thread 7, and ventilating cover 2 medial wall is provided with bottle cap female thread 8, ventilating cover 2 and sample-adding Bottle neck 5 passes through bottle neck screw thread 7 and bottle cap female thread 8 is tightened.

Preferably, as shown in Fig. 3, plug core 4 is by upper end pouring opening 9, middle-end protuberance 10 and lower port 11 3 part group Integrated hollow columnar structures；The length of plug core 4 is set to 6.5-8.0 cm, and internal diameter is set to 2.4-3.2 cm.Wherein, lower end Mouthful 11 length are set to 3.0-3.5 cm, are provided with double-deck, the convex cambered surface filter screen being fixed with one with it inside lower port 11 12, filter screen 12 can effectively filter out in bottle liquid blood media surface because quickly shaking up produced a large amount of foams it is ensured that inclining Form smooth, even curface after the blood agar culture-medium solidification of note；Lower port 11 outer wall is provided with silica gel spiral mouth 13, fills in core 4 Sealing plug-in mounting, good airproof performance, the tight not water clock of laminating are realized with toppling over bottle neck 6 inwall by silica gel spiral mouth 13.Middle-end is protruded The external diameter in portion 10 is set to 3.8-4.2 cm, and length is set to 2.0-3.0 cm, and its external diameter is more than the bore toppling over bottle neck 6, and under it End face is in close contact with the bottleneck edge toppling over bottle neck 6, can control the depth that plug core 4 insertion tilts bottle neck 6.Upper end pouring opening 9 Length be set to 2.0-2.5 cm, upper end pouring opening 9 lateral wall be provided with plug core screw thread 14, closure 3 medial wall is provided with bottle cap Screw thread 15, closure 3 passes through to fill in core screw thread 14 with plug core 4 and bottle cap female thread 15 is tightened, when closure 3 and plug core screw thread After 14 are tightened, no leak source contact can be fully achieved, there is safety leak-proof effect.

Preferably, described sample-adding bottle neck 5 is cylindrical-shaped structure, and the length of bottle neck is set to 3.0-3.5 cm, a diameter of 3.6- 4.0 cm, the angle between the axis of sample-adding bottle neck 5 and horizontal plane is 40-60 °, is conducive to adding respectively in culture medium process for preparation Plant reagent, and carry out sample absorption using utensils such as liquid-transfering guns in the small space of superclean bench after sterilizing so that adding Sample and take liquid operation more convenient, it is to avoid liquid-transfering gun touch inside bottleneck rise cross-contamination.As shown in figure 4, on sample-adding bottle neck 5 Ventilating cover 2 diameter of configuration is set to 4.0-4.5 cm, and ventilating cover 2 capping is provided with the air-vent 19 of the arrangement in concentric circles, breathes freely It is provided with fungi-proofing filter membrane 20 and the annular silicone gasket 21 with radian, fungi-proofing filter membrane 20 is replaceable inside lid 2, and by annular silica gel Packing ring 21 supports to be fixed, and ventilating cover 2 medial wall is provided with screw thread, and lateral wall is provided with the anti-skidding raised line 22 of vertical bar shape.Fungi-proofing filter membrane 20 Be conducive to that the steam in high-temperature sterilization can circulate and antibacterial can not pass through, reach fungi-proofing purpose, and inside bottle 1 after sterilizing Can naturally rapid blood pressure lowering, prevent culture medium from overflowing bottle 1, be easy to sterile storage after sterilizing, preferably play fungi-proofing, anti-pollution mesh , anti-skidding raised line 22 can increase frictional force when turning on ventilating cover 2, the unlatching operation of convenient bottle cap.

Preferably, described to topple over bottle neck 6 be cylindrical-shaped structure, and the length of bottle neck is set to 3.0-3.5 cm, a diameter of 3.0- 3.4 cm, toppling over the angle between bottle neck 6 axis and horizontal plane is 40-70 °.The design at bottle neck angle of inclination meets ergonomic Mechanics, has convenience that is easy to operate, being easy to pour plate and control flow.As shown in Fig. 5, topple over configuration on bottle neck 6 Closure 3 diameter is set to 3.4-4.0 cm, and closure 3 inside top is provided with the circular silica gel pad circle 23 with radian, in closure 3 Side wall is provided with screw thread, and lateral wall is provided with the anti-skidding raised line 22 of vertical bar shape.

As shown in Fig. 6, bottle 1 outer wall is supporting to be provided with dismountable collet 16, and collet 16 is preferably by heat-insulated, transparent The rectangular sheet structure made of silica gel material, vertical two sides of collet 16 are correspondingly provided with adhesive strip 25, two adhesive strip institutes In face contrary (i.e. an adhesive strip 25 is in front, and another adhesive strip 25 is in reverse side), collet 16 is coated on the outer wall of bottle 1 On, and fixation is mutually bonded by the adhesive strip 25 of both sides.In concrete operations, because bottle 1 temperature after autoclaving is higher, Single collet 16 can not meet insulation requirement, therefore collet 16 can arrange multilamellar, is coated on successively on the outer wall of bottle 1； Certainly, also single collet 16 can be arranged thicker, to reach higher effect of heat insulation, the size of collet 16 and bottle 1 Size is adapted, as long as ensure that it is fitted tightly on bottle 1 outer wall.

Preferably, described bottle 1 outer wall is provided with graduation mark 17 and recording label 18, the minimum division value of graduation mark 17 For 50 ml, so that pouring into the amount of culture dish accurately, operation is quick in agar culture medium stream process, and then ensure that agar is put down Nutrition uniformity between plate, the volume scope of bottle 1 is set to 500-800 ml.Bottle 1 bottom is in rectangular structure, and length sets For 9.0-10 cm, width is set to 6.5-7.5 cm, is highly set to 9-12 cm；The inside corresponding tilt in the bottle 1 top left and right sides In ramped shaped, the height on slope is set to 4.0-5.0 cm, and the domatic angle and between horizontal plane in slope is set to 30-50 °, domatic on It is respectively provided with sample-adding bottle neck 5 and topple over bottle neck 6.Take, grasp bottle when the thickness design of bottle 1 is for ease of operator's pour plate With anti-slip in the middle part of body, bottle 1 takes up room little, can be easy to multiple medium bottles and carry out autoclaving process simultaneously；On bottle The inclined plane in portion and the design of both sides tilting adding mouth and pour spout, meet ergonomic designs and require, be easy to culture medium Add in process for preparation or draw animal blood and various reagents, particularly in the small space of superclean bench, using Longer measuring pipette or pipettor carry out aseptic sample-adding, particularly suitable in the operation of sampling, take liquid or sample-adding operation more suitable Handss, convenient, liquid-transfering gun barral of a gun can be avoided to touch the pollution causing inside bottleneck；Secondly, can effective control and grasp pour into The amount of the culture medium in plate, is not likely to produce backflow phenomenon, conveniently pours into, and improves the accuracy rate poured into and success rate, saves time, high Effect.Bottle 1 bottom indent, is designed with antiskid thread 24 bottom outer-edge week, and indent design can avoid bottle 1 attachment to adhere to work Table top, increases the fluency of bottle 1 movement, screw thread 24 can increase the stability of placement, it is to avoid slippage desktop is it is ensured that safety.Institute State bottle 1 to make using Pyrex, plug core 4 main body adopts polypropylene (pp) or Merlon (pc) to make, described bottle cap is (thoroughly Gas lid 2 and closure 3) all to be made using phenolic resin, above-mentioned material all can meet the examination of high pressure steam sterilization and Reusability Test requirement, bottle 1 and plug core 4 are made using the high material of transparency, are conducive to operator to observe the shape of culture medium in bottle 1 State, is easy to observe, controls the volume adding/draw culture medium and reagent, the culture facilitating pouring operation and controlling pour plate Dryweather flow.

Specifically, culture medium bottle dimensional structure of the present utility model is specific as follows, and bottle bottom cuboid length × wide × High respectively 10 cm × 7.0 cm × 9.0 cm, the height of upper end ramp structure is 4.5 cm, the domatic folder with horizontal plane in slope Angle is 40 °, and bottle 1 volume is 600 ml, and the length of sample-adding bottle neck 5 is 3.3 cm, and opening diameter is 3.8 cm, is loaded bottle neck 5 Axis and horizontal plane between angle be 50 °, topple over bottle neck 6 length be 3.3 cm, opening diameter be 3.2 cm, topple over bottle neck Angle between 6 axis and horizontal plane is 50 °, and the length of plug core 4 is 7.3 cm, and inner hollow diameter is 2.8 cm, wherein upper end Pouring opening 9 length is 2.3 cm, and middle-end protuberance 10 length and external diameter are respectively 1.8 cm and 4.0 cm, and lower port 11 length is 3.2 cm, a diameter of 4.2 cm of ventilating cover 2, highly for 3.0 cm, a diameter of 3.6 cm of closure 3, highly for 2.4 cm.

This utility model, when carrying out blood agar culture-medium preparation, is carried out essentially according to following steps:

According to requirement of experiment, required dehydrated medium, reagent and ormal weight distilled water are passed through sample-adding bottle neck 5 and adds bottle Interior, preliminarily solubilised mixes, and bottle neck 6 is toppled in plug core 4 insertion, screwing hermetic lid 3 and ventilating cover 2, carry out high steam and go out respectively Bacterium.In sterilization process, high-temperature steam can enter bottle by air-vent 19 and reach sterilization effect；In the sterilizing later stage, high pressure steams Gradually pressure release in vapour autoclave, makes bottle internal gas pressure and ambient pressure decline by air-vent 19, the culture in bottle simultaneously Base will not overflow because bottle internal gas pressure is too high.Fungi-proofing filter membrane 20 can also make the medicinal liquid after sterilizing keep aseptic condition, reaches no The purpose of bacterium storage.Sterilizing terminates, vapour more than exclusion, and bottle is taken out by pressure release to zero, puts in superclean bench, treats bottle temperature Degree is down in the range of 50 ~ 55 DEG C, and gluing the collet 16(that becomes reconciled around bottle can be according to the temperature bonding different layers of culture medium in bottle The collet of number).Turn on ventilating cover 2, the aseptic de-fiber sheep blood of aseptic addition 5% ~ 10% (v/v), tightens in culture medium Gas lid 2, slowly rotates bottle, mixes culture medium and de- fiber Sanguis caprae seu ovis manually.After mixing unanimously, sealing-plug 3 of outwarding winding, enters promoting the circulation of blood The pouring into of agar culture medium, subpackage, the double cambered surface filter screens 12 in plug core 4, liquid blood in bottle can be effectively filtered out and cultivate base table Face because quickly shaking up produced a large amount of foams it is ensured that forming smooth, even curface after the blood agar culture-medium solidification poured into, Avoiding standing or the efficiency that defoams of heating is low, culture medium the phenomenon of skewness, solidification easily it is ensured that blood agar culture Work efficiency is improve while quality.

It is because of foams a large amount of produced by rotation mixing, same that this utility model can filter liquid blood media surface in bottle When can be further ensured that culture medium and blood reaches optimal mixing state, foam removes quick, efficiently it is ensured that the blood agar pouring into Form smooth, smooth consistent surface after culture medium solidifying.Pour into due to carrying out a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices within the temperature range of regulation, and pour into The amount of plate is quick, accurate, thus avoid the agar in bottle and de- fiber Sanguis caprae seu ovis produce the phenomenon of skewness, agar is put down Between plate, nutritional labeling easily reaches the unified standard of uniformity, improves the accuracy rate poured into and success rate, is follow-up test result Accuracy provide technical guarantee.Secondly, its bottleneck size, angle of inclination and bottle cap are reasonable in design, facilitate liquid culture The autoclave sterilization disinfection of base, is easy to carry out aseptic sample-adding or imbibition operation in superclean bench, conveniently pours into, keep away Exempt from the generation of backflow phenomenon, save time, efficiently, can preferably complete bacterial solids, the configuration of semisolid culturemedium and subpackage, from And mitigate, simplify the workload of lab assistant.

Above example is only exemplary, can't limit to this utility model it should be pointed out that technology for this area For personnel, under technology provided by the utility model enlightenment, the other equivalent modifications made and improvement, it is regarded as The protection domain of utility model.

Claims (10)

1. a kind of medium bottle for preparing blood agar plate, including bottle, bottle outer wall is provided with graduation mark, and its feature exists In: described bottle (1) upper end is provided with respectively the sample-adding bottle neck (5) that both sides tilt to the left and right and topples over bottle neck (6), described dumping bottle Mouth (6) is provided with built-in plug core (4) and closure (3), and wherein plug core (4) realizes seamless seal plug-in mounting with toppling over bottle neck (6) inwall, Plug core (4) lower end is provided with double-deck, convex cambered surface filter screen (12), and plug core (4) upper end is passed through screw thread and screwed admittedly with closure (3) Fixed；Described sample-adding bottle neck (5) is configured with the ventilating cover (2) of the uniform air-vent of capping (19), ventilating cover (2) and sample-adding bottle neck (5) It is tightened by screw thread.

2. the medium bottle for preparing blood agar plate according to claim 1 it is characterised in that: described plug core (4) For hollow columnar structures, by upper end pouring opening (9), middle-end protuberance (10) and the integrated knot of lower port (11) three part group Structure；Wherein, it is provided with the filter screen (12) being fixed with one with it inside lower port (11), lower port (11) outer wall is provided with silica gel spiral Mouth (13), plug core (4) passes through silica gel spiral mouth (13), and realization seals plug-in mounting with toppling over bottle neck (6) inwall；Described middle-end protuberance (10) external diameter is more than the bore toppling over bottle neck (6), and its lower surface is in close contact with the bottleneck edge toppling over bottle neck (6)；Institute State upper end pouring opening (9) outer wall and be provided with plug core screw thread (14), it is tightened by screw thread with closure (3).

3. the medium bottle for preparing blood agar plate according to claim 1 it is characterised in that: described bottle (1) Outer wall is supporting to be provided with dismountable collet (16).

4. the medium bottle for preparing blood agar plate according to claim 3 it is characterised in that: described collet (16) rectangular sheet structure made for silica gel, vertical two sides of collet (16) are correspondingly provided with adhesive strip (25), by viscous Narrow bars (25) wrap collet (16) in bottle (1) outer wall.

5. according to the arbitrary described medium bottle for preparing blood agar plate of claim 1-3 it is characterised in that: described Gas lid (2) capping is provided with the air-vent (19) of the arrangement in concentric circles, is provided with fungi-proofing filter membrane (20) and band arc inside ventilating cover (2) The annular silicone gasket (21) of degree, fungi-proofing filter membrane (20) is passed through annular silicone gasket (21) and is supported fixation, ventilating cover (2) medial wall It is provided with screw thread, lateral wall is provided with the anti-skidding raised line (22) of vertical bar shape.

6. according to the arbitrary described medium bottle for preparing blood agar plate of claim 1-3 it is characterised in that: described close Capping (3) inside top is provided with the circular silica gel pad circle (23) with radian, and closure (3) medial wall is provided with screw thread, and lateral wall sets There is the anti-skidding raised line (22) of vertical bar shape.

7. according to the arbitrary described medium bottle for preparing blood agar plate of claim 1-3 it is characterised in that: described plus Angle between the axis of sample bottle neck (5) and horizontal plane is 40-60 °, topples over the angle between the axis of bottle neck (6) and horizontal plane For 40-70 °.

8. the medium bottle for preparing blood agar plate according to claim 1 it is characterised in that: described bottle (1) Bottom is in rectangular structure, and the inside corresponding tilt in the top left and right sides is in ramped shaped, is respectively provided with sample-adding bottle neck on slope is domatic And topple over bottle neck (6) (5).

9. the medium bottle for preparing blood agar plate according to claim 8 it is characterised in that: described bottle (1) The domatic angle and between horizontal plane in slope is set to 30-50 °.

10. the medium bottle for preparing blood agar plate according to claim 1 it is characterised in that: described bottle (1) Made using Pyrex, plug core (4) main body is made using polypropylene or Merlon, and closure (3) and ventilating cover (2) are all adopted Made with phenolic resin.