CN207391419U - Cell culture fluid sterile sampling device - Google Patents
Cell culture fluid sterile sampling device Download PDFInfo
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- CN207391419U CN207391419U CN201721366939.5U CN201721366939U CN207391419U CN 207391419 U CN207391419 U CN 207391419U CN 201721366939 U CN201721366939 U CN 201721366939U CN 207391419 U CN207391419 U CN 207391419U
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- bottle
- probe tube
- caching
- communicated
- sampling
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Abstract
The utility model discloses a kind of cell culture fluid sterile sampling devices, including storage bottle, the first probe tube is provided with and communicated in storage bottle, the other end of first probe tube is provided with and communicated with caching bottle, and the first check valve and first switch valve are arranged at intervals on the first probe tube between storage bottle and caching bottle；Be provided with and communicated in caching bottle into air outlet pipe and the second probe tube, extend to the top of caching bottle inner cavity into air outlet pipe one end and the other end is provided with and communicated with syringe, into air outlet pipe on be arranged at intervals with second switch valve and air filter；Second probe tube one end extends to caching bottle intracavity bottom and its other end is provided with and communicated with sampling container, and the second check valve and the 3rd switch valve are arranged at intervals on the second probe tube.The utility model advantage is the purpose that sterile sampling is realized using differential pressure principle and first, second check valve, greatly reduces the contaminated probability of cell, improves the accuracy of experimental data, the frequent sampling especially suitable for cell.
Description
Technical field
The utility model is related to laboratory apparatus, more particularly, to a kind of cell culture fluid sterile sampling device.
Background technology
Cell culture technology is most crucial, most basic technology in biotechnology, is drug development research and bio-pharmaceuticals
Basis.During experiment, experimenter carries out in vitro culture breeding usually in reactor to zooblast.Since cells ex vivo is given birth to
Long environment breeds it and antibody expression tool has a significant impact, thus the close of regular sampling monitoring cell is needed in reproductive process
The indexs such as degree, motility rate, antibody-secreting and amino acid consumption.During sampling, the sampling of experimenter's generally use sterile sampling device is existing
It is usually that equipment is moved into gnotobasis with sterile sampling method, opens lid and be sampled, it is comparatively laborious due to operating,
And it frequently opens lid and be easy to cause pollution, if being also exactly that container volume is larger, to the area of carrying and gnotobasis
Also there are requirements at the higher level, because of this experimental provision of the invention.
The content of the invention
The utility model aim is to provide a kind of cell culture fluid sterile sampling device, which can
Effectively ensure sterile sampling, greatly reduce the contaminated probability of cell.
To achieve the above object, the utility model takes following technical proposals：
Cell culture fluid sterile sampling device described in the utility model, including the storage bottle being connected with reactor,
The first probe tube is provided with and communicated in the storage bottle, the other end of first probe tube extends outward storage bottle and connects
It is logical to be provided with caching bottle, be arranged at intervals on the first probe tube between storage bottle and the caching bottle the first check valve and
First switch valve；It is provided with and communicated in the caching bottle into air outlet pipe and the second probe tube, it is described to be extended into air outlet pipe one end
Cache the top of bottle inner cavity and the other end is provided with and communicated with syringe, into air outlet pipe on be arranged at intervals with second switch valve and air
Filter；One end of second probe tube extends to caching bottle intracavity bottom and its other end is provided with and communicated with sampling container,
The second check valve and the 3rd switch valve are arranged at intervals on second probe tube.
Described first probe tube one end extends to the storage bottle intracavity bottom, and the other end is extended in the caching bottle
The top of chamber.
The sampling container is centrifuge tube or sampling injector.
The utility model advantage is clever structure, and sterile take is realized using differential pressure principle and first, second check valve
The purpose of sample avoids extra sampling, greatly reduces the contaminated probability of cell, improves the accuracy of experimental data, especially
Suitable for the frequent sampling of cell.Its specific sampling process is as follows：The 3rd switch valve is closed, first switch valve and second is opened and opens
Valve is closed, is outwards evacuated with the first syringe so that negative pressure occurs in caching bottle, and the cell liquid in storage bottle enters through the first probe tube
It caches in bottle；First switch valve is then shut off, opens the 3rd switch valve, is supplied gas, cached in bottle into caching bottle by syringe
Liquid pressure differential effect under from the second probe tube flow into sampling container in.
Description of the drawings
Fig. 1 is the structure diagram of the utility model.
Specific embodiment
As shown in Figure 1, cell culture fluid sterile sampling device described in the utility model, including being connected with reactor
Storage bottle 1, be provided with and communicated with the first probe tube 2 in storage bottle 1, the other end of the first probe tube 2 extends outward storage bottle
1 and the caching bottle 3 with sealing-plug is provided with and communicated with, is spaced on the first probe tube 2 between storage bottle 1 and caching bottle 3
It is provided with the first check valve 4 and first switch valve 5；It is provided with and communicated in caching bottle 3 into 6 and second probe tube 7 of air outlet pipe, disengaging
6 one end of tracheae extends to the top of caching bottle 3 inner cavity and the other end is provided with and communicated with syringe 8, into air outlet pipe 6 on be arranged at intervals
There are a second switch valve 9 and air filter 10, air filter 10 ensures that syringe 8 is supplied gas quality；One end of second probe tube 7
（That is inlet end）Extend to caching 3 intracavity bottom of bottle and its other end（That is outlet end）It is provided with and communicated with sampling container 11
（Can be centrifuge tube or sampling injector）, the second check valve 12 and the 3rd switch valve 13 are arranged at intervals on the second probe tube 7,
First check valve 4 ensures that cell liquid flow direction from storage bottle 1 is cached in bottle 3, and the second check valve 12 ensures the cell in caching bottle 3
Liquid stream enters in sampling container 11, prevents cell liquid from flowing backwards, it is ensured that the gnotobasis in caching bottle 3.
It is polluted to reduce, 2 one end of the first probe tube extends to 1 intracavity bottom of storage bottle, another to extend in caching bottle 3
The top of chamber（That is 2 inlet end of the first probe tube extends to 1 intracavity bottom of storage bottle, and outlet end is extended in caching bottle 3
Above chamber）, prevent cell liquid from remaining at 2 liquid outlet of the first probe tube.
Using present embodiment, there is volume markings line on syringe 8, therefore the rate of air sucked in required of syringe 8 and gas pushing quantity are controllable
System, i.e., can control sampling amount by syringe 8, prevent the cell liquid in caching bottle 3 floods from being taken into air outlet pipe end and second
Sample pipe greatly reduces the contaminated probability of cell liquid, it is ensured that sterile sampling.
In use, closing the 3rd switch valve 13, open first switch valve 5 and second switch valve 9, with the first syringe 8 to
There is negative pressure in outer pumping, caching bottle 3 inner cavity, the cell liquid in storage bottle 1 through the first probe tube 2 into caching bottle 3, during pumping
Ensure to cache the liquid level of 3 inner cavity of bottle while less than the liquid outlet of the first probe tube 2 and into air outlet pipe 6, further reduce cell liquid
Contaminated probability；First switch valve 5 is then shut off, opens the 3rd switch valve 13, is sent by syringe 8 to 3 inner cavity of caching bottle
Gas, 3 chamber pressure of caching bottle are more than atmospheric pressure so that the cell liquid in caching bottle 3 is under pressure differential effect from the second probe tube 7
It flows into sampling container 11 and sampling operation can be completed；Second switch valve 9 and the 3rd switch valve 13 are closed after the completion of sampling, is convenient for
Next sub-sampling.
Claims (3)
1. a kind of cell culture fluid sterile sampling device, including the storage bottle being connected with reactor（1）, it is characterised in that：
The storage bottle（1）Inside it is provided with and communicated with the first probe tube（2）, first probe tube（2）The other end extend outward storage
Deposit bottle（1）And it is provided with and communicated with caching bottle（3）, positioned at storage bottle（1）With the caching bottle（3）Between the first probe tube（2）
On be arranged at intervals with the first check valve（4）With first switch valve（5）；The caching bottle（3）It is inside provided with and communicated with into air outlet pipe（6）
With the second probe tube（7）, it is described into air outlet pipe（6）One end extends to caching bottle（3）The top of inner cavity and other end connection is set
There is syringe（8）, into air outlet pipe（6）On be arranged at intervals with second switch valve（9）And air filter（10）；Second sampling
Pipe（7）One end extend to caching bottle（3）Intracavity bottom and its other end is provided with and communicated with sampling container（11）, the second probe tube
（7）On be arranged at intervals with the second check valve（12）With the 3rd switch valve（13）.
2. cell culture fluid sterile sampling device according to claim 1, it is characterised in that：First probe tube
（2）One end extends to the storage bottle（1）Intracavity bottom, the other end extend to the caching bottle（3）The top of inner cavity.
3. cell culture fluid sterile sampling device according to claim 1 or 2, it is characterised in that：The sampling container
（11）For centrifuge tube or sampling injector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721366939.5U CN207391419U (en) | 2017-10-23 | 2017-10-23 | Cell culture fluid sterile sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721366939.5U CN207391419U (en) | 2017-10-23 | 2017-10-23 | Cell culture fluid sterile sampling device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207391419U true CN207391419U (en) | 2018-05-22 |
Family
ID=62329483
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721366939.5U Active CN207391419U (en) | 2017-10-23 | 2017-10-23 | Cell culture fluid sterile sampling device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207391419U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113481082A (en) * | 2021-07-13 | 2021-10-08 | 深圳赛桥生物创新技术有限公司 | Sterile cell sampling device and method based on injector |
| CN113528302A (en) * | 2021-07-13 | 2021-10-22 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
-
2017
- 2017-10-23 CN CN201721366939.5U patent/CN207391419U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113481082A (en) * | 2021-07-13 | 2021-10-08 | 深圳赛桥生物创新技术有限公司 | Sterile cell sampling device and method based on injector |
| CN113528302A (en) * | 2021-07-13 | 2021-10-22 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
| WO2023284580A1 (en) * | 2021-07-13 | 2023-01-19 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
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