CN216404420U

CN216404420U - Cell culture device capable of monitoring cell culture liquid environment

Info

Publication number: CN216404420U
Application number: CN202123250621.XU
Authority: CN
Inventors: 姜树原
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-12-22
Filing date: 2021-12-22
Publication date: 2022-04-29
Anticipated expiration: 2031-12-22

Abstract

The utility model discloses a cell culture device capable of monitoring a cell culture liquid environment, and belongs to the field of cell culture. The utility model provides a can monitor cell culture liquid environment's cell culture device, includes the culture dish, the culture dish upside is fixed with the screw ring, the screw ring internal rotation installs the threaded rod, the threaded rod downside is equipped with the sampler barrel, the outer terminal surface of sampler barrel is fixed with the sampling tube that matches with the culture dish position, the sampler barrel upper end is fixed with the air pump, the culture dish lower extreme is fixed with the conveyer pipe, the conveyer pipe outer end is connected with the observation board. The utility model can sample the culture solution in the culture dish, avoid picking up cells and ensure the normal growth of the cells.

Description

Cell culture device capable of monitoring cell culture liquid environment

Technical Field

The utility model relates to the field of cell culture, in particular to a cell culture device capable of monitoring a cell culture liquid environment.

Background

The in vitro cell culture technology is to inoculate mammalian cells in a liquid culture medium in a cell culture container, wherein the liquid culture medium contains various nutrients necessary for cell growth and propagation, so that the mammalian cells can grow and proliferate. In scientific research, it is often necessary to study the effect of a particular substance in a cell culture fluid on the processes of cell growth, division, proliferation, etc. at a particular concentration. And then need pay close attention to the change of culture solution environment constantly, current culture apparatus when using, needs the staff to open the incubator door every an end time, takes out the sample that awaits measuring among the culture apparatus, and is comparatively loaded down with trivial details, the increase is at staff's work burden to cause the change of the interior gaseous environment of incubator easily when opening the incubator door, influence the accuracy of experimental result.

Patent document CN215050382U discloses in the prior art provides a cell culture apparatus capable of monitoring the liquid environment of cell culture; the device passes through the motor and drives second screw rod, first screw rod, two sliders, dwang, movable plate, U type support frame in proper order and moves up to make according to the briquetting and break away from the pressure to the hose, and then make things convenient for the culture solution in the incubator to monitor through the hose inflow monitor in and observe, need not the manual apron of opening the incubator of staff and take out liquid and monitor, reduce the change of gaseous environment in the incubator, increase the accuracy of experimental result.

Although the device has more beneficial effects, the following problems still exist: the monitoring mode of cells mainly takes monitoring culture solution as a main mode, the growth condition of the cells is deduced according to the types and the contents of substances in the culture solution, the original device not only takes out the culture solution in the sampling process, but also takes out more cells, and the growth condition of the cells is influenced in the guiding and conveying process. In view of this, we propose a cell culture apparatus that can monitor the liquid environment of cell culture.

SUMMERY OF THE UTILITY MODEL

1. Technical problem to be solved

The present invention is directed to a cell culture apparatus capable of monitoring the liquid environment of cell culture, so as to solve the above problems in the prior art.

2. Technical scheme

The utility model provides a can monitor cell culture liquid environment's cell culture device, includes the culture dish, the culture dish upside is fixed with the screw ring, the screw ring internal rotation installs the threaded rod, the threaded rod downside is equipped with the sampler barrel, the outer terminal surface of sampler barrel is fixed with the sampling tube that matches with the culture dish position, the sampler barrel upper end is fixed with the air pump, the culture dish lower extreme is fixed with the conveyer pipe, the conveyer pipe outer end is connected with the observation board.

Preferably, two connecting rods which are symmetrically arranged front and back are fixed between the upper end of the culture dish and the lower end of the threaded ring, and the gap between the two connecting rods is matched with the diameter of the conveying pipe.

Preferably, the connection part of the sampling tube and the conveying pipe is arranged in a funnel shape.

Preferably, a first valve and a second valve are respectively arranged in the sampling pipe and the conveying pipe.

Preferably, the lower end of the threaded rod is provided with a limiting rotating groove, and the upper end of the air pump is fixed with a limiting rotating block.

3. Advantageous effects

Compared with the prior art, the utility model has the advantages that:

1. according to the utility model, air in the sampling cylinder is led out through the air pump, the culture solution in the culture dish is guided into the sampling cylinder through the sampling tube, then outside air is led into the sampling cylinder through the air pump, and the culture solution in the sampling tube is guided into the observation plate through the conveying pipe, so that the type and the content of substances in the culture solution in the observation plate can be conveniently monitored through the external monitoring device.

2. According to the utility model, the heights of the sampling tube and the sampling tube can be adjusted through the threaded ring and the threaded rod, so that the height of the sampling tube is matched with the depth of the culture solution in the culture dish, and the sampling tube is prevented from sucking cells in the culture dish.

Drawings

FIG. 1 is a schematic view of the overall external structure of the present invention;

FIG. 2 is a schematic view of the external structure of the culture dish of the present invention;

FIG. 3 is a schematic view of the external structure of the sampling tube of the present invention;

FIG. 4 is a schematic view of the inner part of the sampling tube of the present invention;

the reference numbers in the figures illustrate: 1. a culture dish; 2. a threaded ring; 3. a sampling tube; 4. a connecting rod; 5. a threaded rod; 6. a sampling tube; 7. a delivery pipe; 8. a first valve; 9. a second valve; 10. a limiting rotation groove; 11. a limiting rotating block; 12. an air pump; 13. and (4) observing the plate.

Detailed Description

Referring to fig. 1-4, the present invention provides a technical solution:

the utility model provides a can monitor cell culture device of cell culture fluid environment, includes

culture dish

1, 1 upside of culture dish is fixed with threaded ring 2, and threaded rod 5 is installed to 2 internal rotations of threaded ring, and 5 downside of threaded rod are equipped with

sampler barrel

3, and 3 outer terminal surfaces of sampler barrel are fixed with the sampling tube 6 that matches with 1 position of culture dish, and 3 upper ends of sampler barrel are fixed with

air pump

12, and 1 lower extremes of culture dish are fixed with

conveyer pipe

7, and 7 outer ends of conveyer pipe are connected with observation board 13.

Specifically, two connecting rods 4 which are symmetrically arranged in front and back are fixed between the upper end of the culture dish 1 and the lower end of the threaded ring 2, and the gap between the two connecting rods 4 is matched with the diameter of the conveying pipe 7; the direction of movement of the sampling tube 6 can be limited.

Furthermore, the connection part of the sampling tube 6 and the conveying pipe 7 is arranged in a funnel shape; ensuring the sample delivery of the sampling tube 6 is complete.

Furthermore, a first valve 8 and a second valve 9 are respectively arranged in the sampling pipe 6 and the conveying pipe 7; the opening and closing of the sampling tube 6 and the delivery tube 7 can be controlled.

Furthermore, the lower end of the threaded rod 5 is provided with a limit rotating groove 10, and the upper end of the air pump 12 is fixed with a limit rotating block 11; the threaded rod 5 is prevented from driving the sampling tube 6 to rotate.

The working principle is as follows: when the sampling tube is used, the threaded rod 5 is rotated in the threaded ring 2, so that the threaded rod 5 moves up and down, the two connecting rods 4 limit the movement directions of the conveying tube 7 and the sampling tube 6, the threaded rod 5 drives the sampling tube 6 to move up and down, the lower end of the sampling tube 6 is deeply matched with the culture solution in the culture dish 1, most cells are in an adherent growth state, so that the sampling tube 6 is prevented from contacting with the cells, the first valve 8 is opened, the second valve 9 is closed, the air pump 12 is started through the external control device, air is introduced through the lower end channel of the air pump 12 and is exhausted through the outer end channel of the air pump 12, the air in the sampling tube 3 is led out, the inner cavity of the sampling tube 3 is in a negative pressure state, the culture solution in the culture dish 1 is guided into the sampling tube 3 through the sampling tube 6, and the first valve 8 is closed, Open second valve 9, introduce the air through 12 outer end passageways of air pump, and discharge the air through 12 lower extreme passageways of air pump, increase 3 inner chamber cavity pressures of sampling tube, thereby through conveyer pipe 7, can be with in the culture solution propelling movement observation board 13 in the sampling tube 3, in observation board 13 located external monitoring devices, monitor the material kind and the content of culture solution in observation board 13 through external monitoring devices, and then deduce the growth conditions of cell.

Claims

1. A cell culture device capable of monitoring cell culture liquid environment comprises a culture dish (1), and is characterized in that: culture dish (1) upside is fixed with screw ring (2), threaded rod (5) are installed to screw ring (2) internal rotation, threaded rod (5) downside is equipped with sampler barrel (3), sampler barrel (3) outer terminal surface is fixed with sampling tube (6) with culture dish (1) position matching, sampler barrel (3) upper end is fixed with air pump (12), culture dish (1) lower extreme is fixed with conveyer pipe (7), conveyer pipe (7) outer end is connected with observation board (13).

2. A cell culture apparatus according to claim 1, wherein the apparatus comprises: two connecting rods (4) which are symmetrically arranged front and back are fixed between the upper end of the culture dish (1) and the lower end of the threaded ring (2), and the gap between the two connecting rods (4) is matched with the diameter of the conveying pipe (7).

3. A cell culture apparatus according to claim 1, wherein the apparatus comprises: the connecting part of the sampling tube (6) and the conveying pipe (7) is arranged in a funnel shape.

4. A cell culture apparatus according to claim 1, wherein the apparatus comprises: and a first valve (8) and a second valve (9) are respectively arranged in the sampling pipe (6) and the conveying pipe (7).

5. A cell culture apparatus according to claim 1, wherein the apparatus comprises: the lower end of the threaded rod (5) is provided with a limiting rotating groove (10), and the upper end of the air pump (12) is fixed with a limiting rotating block (11).