CN220224168U

CN220224168U - Cell bioreactor

Info

Publication number: CN220224168U
Application number: CN202321417477.0U
Authority: CN
Inventors: 阮青华; 阮青松
Original assignee: Wuxue Fuying Electromechanical Equipment Co ltd
Current assignee: Wuxue Fuying Electromechanical Equipment Co ltd
Priority date: 2023-06-05
Filing date: 2023-06-05
Publication date: 2023-12-22
Anticipated expiration: 2033-06-05

Abstract

The utility model relates to a cell bioreactor, which comprises a tank body, wherein a working cavity is arranged in the tank body, a stirrer is arranged in the working cavity, a multi-point sampling mechanism is arranged on the left side of the working cavity, an automatic sampling mechanism is arranged on the left side of the top end of the tank body, the multi-point sampling mechanism comprises an L-shaped pipe, a hand wheel, a spring, a telescopic pipe, a roller and a first sealing ring, the design of the multi-point sampling mechanism is that when the cell bioreactor is used, culture solution can be firstly added into the tank body, when the cell bioreactor is used, the L-shaped pipe can be firstly rotated clockwise to drive the telescopic pipe to slide towards the inner side of the bottom of the L-shaped pipe when the culture solution is periodically sampled, until the front end of the L-shaped pipe contacts the front end of the working cavity and then stops rotating, at the moment, the automatic sampling mechanism can be started to sample the culture solution by the L-shaped pipe, the culture solution can be driven to enter the telescopic pipe through a sampling port under the negative pressure effect of the inner part of the L-shaped pipe, and then is output to be collected through the top end of the L-shaped pipe.

Description

Cell bioreactor

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell bioreactor.

Background

The reactor is a large cell culture system, and the culture of cells and the separation of products are carried out by controlling the conditions of temperature, airflow velocity, rotating speed, oxygen capacity and the like. In the process of using the cell bioreactor, the staff is required to sample, detect and adjust the culture solution in the reactor frequently, so that the temperature, the pH value and the dissolved oxygen degree of the reactor can ensure that cells are in a better culture environment, when sampling is carried out, the staff is required to extract the culture solution through a sampling tube preset by the reactor, the intermediate solution is taken to be added into a reagent tube, in the use process of the existing cell bioreactor, although a stirring device is often arranged in the existing reactor, in order to ensure the accuracy of data, the sampling and detection of multiple points in the reactor are still required, the existing sampling is carried out only by sampling at a single position through the sampling tube, the accuracy is lower, the staff is required to carry out manual operation during sampling, the sampling is also carried out by using needle tube drawing, the sampling efficiency is lower, and therefore the practicability is poor.

Disclosure of Invention

Aiming at the problems in the prior art, the utility model provides a cell bioreactor to solve the technical problems of low sampling precision and low sampling efficiency in the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a cell bioreactor, includes the jar body, and the inside of jar body is provided with the working chamber, and the inside of working chamber is provided with the agitator, the left side of working chamber is provided with multi-point position sampling mechanism, and the top left side of jar body is provided with automatic sampling mechanism, multi-point position sampling mechanism includes L type pipe, hand wheel, spring, flexible pipe, gyro wheel and first sealing washer, and the top left side of working chamber is provided with the rotation hole, and the top outside and the rotation hole of L type pipe are rotated and are connected, and the hand wheel suit is on the top of L type pipe, and the bottom right-hand member of L type pipe is provided with the spout, and the left part and the spout sliding connection of flexible pipe, the bottom right side of flexible pipe are provided with the sample connection, and the left and right sides of spring are connected with the left end and the right-hand member of flexible pipe respectively, and the right-hand member of gyro wheel is installed at the right-hand member of flexible pipe, and the bottom outer end of gyro wheel is hugged closely, and the right side of spout is provided with first seal groove, and first sealing washer cooperation is installed in first seal groove.

Preferably, the automatic sampling mechanism comprises a sampler, two groups of check valves, an electric telescopic rod, a piston plate and a second sealing ring, wherein the sampler is arranged at the top end of the L-shaped pipe, a sampling cavity is arranged in the sampler, a liquid inlet and a liquid outlet are respectively arranged at the bottom end middle part and the bottom end of the left end of the sampling cavity, the liquid inlet is rotationally connected with the top end of the L-shaped pipe and is communicated with the inside of the L-shaped pipe, the two groups of check valves are respectively matched and arranged in the liquid inlet and the liquid outlet, the electric telescopic rod is arranged at the middle part of the top end of the sampler, the top end of the sampling cavity is provided with a sliding hole, the output end of the electric telescopic rod passes through the sliding hole and is connected with the middle part of the top end of the piston plate, the second sealing groove is arranged at the outer end of the piston plate, the second sealing ring is matched and arranged in the second sealing groove, and the left end of the liquid outlet is provided with a sealing liquid outlet mechanism.

In a further preferred embodiment, the sealed liquid outlet mechanism includes drain pipe, taper plug and dust-free cover, and the input of drain pipe communicates with the liquid outlet of sampling chamber left end bottom, and the input of taper plug communicates with the output of drain pipe, and dust-free cover slip-on is in the outside of taper plug, and the setting of sealed liquid outlet mechanism can play sealed dustproof antibacterial effect when carrying out the sample of culture solution.

In a further preferred embodiment, the left end of the sampler is provided with a clip, the taper plug is slidably connected with the clip, and the clip is arranged to more facilitate hanging the taper plug.

In a further preferred embodiment, three groups of elastic membrane flaps are uniformly arranged in the sampling port, and the three groups of elastic membrane flaps can not enter the culture solution until the inside of the L-shaped tube is in a negative pressure state.

In a further preferred embodiment, a dial is provided on the left side of the top end of the tank, and the dial is provided to more intuitively observe and adjust the rotation angle of the L-shaped tube.

In a further preferred aspect, the outer side of the roller is provided with a polyurethane sleeve, and the polyurethane sleeve can reduce abrasion and protect the tank body when the roller abuts against the outer wall of the tank body.

Compared with the prior art, the utility model provides a cell bioreactor which has the following beneficial effects:

the design of the multi-point sampling mechanism can firstly add culture solution into the tank body when the cell bioreactor is used, the L-shaped tube can be rotated clockwise when the culture solution is periodically sampled, so that the telescopic tube is driven to slide towards the inner side of the bottom of the L-shaped tube until the front end of the L-shaped tube contacts the front end of the working cavity, the automatic sampling mechanism can be started to sample the culture solution by using the L-shaped tube, the culture solution can be driven to enter the telescopic tube through the sampling port under the action of negative pressure in the L-shaped tube, then the culture solution is output and collected through the top end of the L-shaped tube, thereby completing the sampling of the culture solution at one point, then the L-shaped tube is rotated anticlockwise by a certain angle, the telescopic tube can be driven to slide towards the right side under the action of the spring, then the automatic sampling mechanism is started to sample the culture solution, thereby completing the sampling at the second point, after several times of circulation, the sampling of the last point is carried out until the rear end of the L-shaped tube contacts the rear end of the working cavity, thereby more conveniently sampling and the sampling and detecting the plurality of points of the culture solution.

The automatic sampling mechanism design, when using L type pipe to take a sample, can operate the reciprocal output of electric telescopic handle and withdraw and drive the piston board and carry out reciprocal slip, will open the check valve of bottom and close the check valve of left end when the piston board rises, will take a vacuum to L type pipe this moment, will utilize the sample connection to extract the culture solution under its inside negative pressure effect, until the culture solution enters into the sampler inside through L type pipe, will close the check valve of bottom and open the check valve of left end when the piston board descends, will extrude the culture solution in the sampling chamber at this moment out the sampler, begin to collect the culture solution through sealed drain mechanism, thereby more convenient extraction sample to the culture solution, need not use the syringe to operate by hand, improve the sample and the detection efficiency of culture solution.

The sealed liquid outlet mechanism design, when taking a sample to the internal culture solution of jar, can slide dustless lid earlier and open, can insert the rubber buffer of reagent pipe with the toper plug and go deep into the reagent intraductal this moment, can operate automatic sampling mechanism and take a sample the culture solution and extrude the sample intracavity with it this moment, the culture solution of extrusion will enter into the drain pipe to in the reagent intraductal through the toper plug, whole sampling process is in sealing state, can not cause the pollution of sample and the internal culture solution of jar, further improves sample and detection precision.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the present utility model;

FIG. 2 is a schematic view of a front cross-sectional structure of the present utility model;

FIG. 3 is a schematic view showing the bottom structure of an L-shaped pipe according to the present utility model;

FIG. 4 is a schematic view of the front cross-sectional structure of an L-shaped pipe according to the present utility model;

fig. 5 is a schematic diagram of the front cross-sectional structure of the sampler in the present utility model.

In the figure: 1. a tank body; 2. a working chamber; 3. a stirrer; 4. an L-shaped tube; 5. a hand wheel; 6. a spring; 7. a telescopic tube; 8. a roller; 9. a first seal ring; 10. a sampling port; 11. a sampler; 12. a one-way valve; 13. an electric telescopic rod; 14. a piston plate; 15. a second seal ring; 16. a sampling cavity; 17. a liquid outlet pipe; 18. a conical plug; 19. a dust-free cover; 20. a clip; 21. an elastic membrane flap; 22. a dial; 23. a polyurethane sleeve.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Example 1:

referring to fig. 1-5, a cell bioreactor comprises a tank body 1, a working cavity 2 is arranged in the tank body 1, a stirrer 3 is arranged in the working cavity 2, a multi-point sampling mechanism is arranged on the left side of the working cavity 2, an automatic sampling mechanism is arranged on the left side of the top end of the tank body 1, the multi-point sampling mechanism comprises an L-shaped pipe 4, a hand wheel 5, a spring 6, a telescopic pipe 7, a roller 8 and a first sealing ring 9, a rotating hole is arranged on the left side of the top end of the working cavity 2, the outer side of the top of the L-shaped pipe 4 is rotationally connected with the rotating hole, the hand wheel 5 is sleeved at the top end of the L-shaped pipe 4, a sliding groove is arranged at the right end of the bottom of the L-shaped pipe 4, the left part of the telescopic pipe 7 is in sliding connection with the sliding groove, a sampling port 10 is arranged on the right side of the bottom end of the telescopic pipe 7, the left and right ends of the spring 6 are respectively connected with the left end of the sliding groove and the right end of the telescopic pipe 7, the roller 8 is mounted at the right end of the telescopic pipe 7, the right end of the roller 8 is tightly attached to the outer end of the bottom of the working cavity 2, a first sealing groove is arranged on the right side of the sliding groove, a first sealing groove 9 is matched with a third sealing groove 10, a uniform sealing groove is arranged in the inner side of the sealing groove 21, a uniform sealing groove 21 is arranged in the sealing groove 21, and a uniform sealing groove 21 is arranged inside the elastic valve set is arranged inside the sealing groove 21, and is arranged inside the sealing groove 21; when the cell bioreactor is used, culture solution can be added into the tank body 1 firstly, when the culture solution is periodically sampled, the L-shaped tube 4 can be rotated clockwise, so that the telescopic tube 7 is driven to slide towards the inner side of the bottom of the L-shaped tube 4, until the front end of the L-shaped tube 4 is contacted with the front end of the working cavity 2, the automatic sampling mechanism can be started to sample the culture solution by using the L-shaped tube 4, the culture solution can be driven to enter the telescopic tube 7 through the sampling port 10 under the negative pressure effect in the L-shaped tube 4, then the culture solution is output and collected through the top end of the L-shaped tube 4, thereby completing sampling of the culture solution at one point, then the L-shaped tube 4 is rotated anticlockwise by a certain angle, at the moment, the telescopic tube 7 is driven to slide towards the right side under the effect of the spring 6, then the automatic sampling mechanism is started to sample the culture solution, thus completing sampling of a second point, after several times of operation, the sampling is performed until the rear end of the L-shaped tube 4 is contacted with the rear end of the working cavity 2, the last point is sampled, thus more convenient and sampling precision of the culture solution is improved.

Example 2:

referring to fig. 1-5, when the L-shaped tube 4 is used for sampling, the electric telescopic rod 13 can be operated to reciprocate to output and retract to drive the piston plate 14 to reciprocate, when the piston plate 14 rises, the check valve 12 at the bottom end is opened and the check valve 12 at the left end is closed, at this time, the L-shaped tube 4 is vacuumized, the culture solution is pumped by the sampling port 10 under the internal negative pressure until the culture solution enters the sampler 11 through the L-shaped tube 4, when the piston plate 14 descends, the check valve 12 at the bottom end is closed and the check valve 12 at the left end is opened, at this time, the culture solution in the sampling cavity 16 is pressed out of the sampler 11, and collection of the culture solution is started through the sealed liquid outlet mechanism, so that the culture solution is pumped and sampled more conveniently, the operation of a syringe is not needed, and the sampling and detection efficiency of the culture solution is improved.

Example 3:

referring to fig. 1-5, on the basis of embodiment 2, when the culture solution in the tank 1 is sampled, the dust-free cover 19 can be slid and opened first, at this time, the conical plug 18 can be inserted into the rubber plug of the reagent tube and go deep into the reagent tube, at this time, the automatic sampling mechanism can be operated to sample the culture solution and press the culture solution out of the sampling cavity 16, the pressed culture solution will enter the liquid outlet pipe 17 and enter the reagent tube through the conical plug 18, the whole sampling process is in a sealed state, no pollution is caused to the sample and the culture solution in the tank 1, and the sampling and detecting precision is further improved.

In all the above mentioned solutions, in which the connection between two components can be chosen according to the actual situation, a welded, bolt-and-nut-fitted connection, a bolt-or-screw connection or other known connection means, which are not described in detail herein, where reference is made to a written fixed connection, the preferred consideration is welding, although embodiments of the utility model have been shown and described, it will be understood by those skilled in the art that numerous variations, modifications, substitutions and alterations can be made to these embodiments without departing from the principle and spirit of the utility model, the scope of which is defined by the appended claims and their equivalents.

Claims

1. The utility model provides a cell bioreactor, includes a jar body (1), and the inside of jar body (1) is provided with working chamber (2), and the inside of working chamber (2) is provided with agitator (3), its characterized in that: the left side of working chamber (2) is provided with multi-point position sampling mechanism, and the top left side of jar body (1) is provided with automatic sampling mechanism, multi-point position sampling mechanism includes L type pipe (4), hand wheel (5), spring (6), flexible pipe (7), gyro wheel (8) and first sealing washer (9), and the top left side of working chamber (2) is provided with the rotation hole, and the top outside and the rotation hole of L type pipe (4) rotate to be connected, and hand wheel (5) suit is at the top of L type pipe (4), and the bottom right-hand member of L type pipe (4) is provided with the spout, and the left part and the spout sliding connection of flexible pipe (7), the bottom right side of flexible pipe (7) is provided with sample connection (10), and the left and right ends of spring (6) are connected with the left end of spout and the right-hand member of flexible pipe (7) respectively, and the right-hand member of gyro wheel (8) are installed at the right-hand member of flexible pipe (7), and the bottom outer end of working chamber (2) are hugged closely, and the right side of spout is provided with first seal groove, and first sealing washer (9) are installed in first seal groove cooperation.

2. A cellular bioreactor according to claim 1, wherein: the automatic sampling mechanism comprises a sampler (11), two groups of one-way valves (12), an electric telescopic rod (13), a piston plate (14) and a second sealing ring (15), wherein the sampler (11) is arranged at the top end of an L-shaped pipe (4), a sampling cavity (16) is arranged in the sampler (11), a liquid inlet and a liquid outlet are respectively arranged at the bottom end middle part and the left end bottom part of the sampling cavity (16), the liquid inlet is rotationally connected with the top end of the L-shaped pipe (4) and is communicated with the inside of the L-shaped pipe (4), the two groups of one-way valves (12) are respectively matched and arranged in the liquid inlet and the liquid outlet, the electric telescopic rod (13) is arranged at the top end middle part of the sampler (11), the top end of the sampling cavity (16) is provided with a sliding hole, the output end of the electric telescopic rod (13) is connected with the top end middle part of the piston plate (14) through the sliding hole, the outer end of the piston plate (14) is provided with a second sealing groove, the second sealing ring (15) is matched and is arranged in the second sealing groove, and the left end of the liquid outlet is provided with a sealing mechanism.

3. A cellular bioreactor according to claim 2, wherein: the sealed liquid outlet mechanism comprises a liquid outlet pipe (17), a conical plug (18) and a dust-free cover (19), wherein the input end of the liquid outlet pipe (17) is communicated with a liquid outlet at the bottom of the left end of the sampling cavity (16), the input end of the conical plug (18) is communicated with the output end of the liquid outlet pipe (17), and the dust-free cover (19) is sleeved on the outer side of the conical plug (18) in a sliding mode.

4. A cellular bioreactor according to claim 2, wherein: the left end of the sampler (11) is provided with a clamp (20), and the conical plug (18) is in sliding connection with the inside of the clamp (20).

5. A cellular bioreactor according to claim 1, wherein: three groups of elastic membrane flaps (21) are uniformly arranged in the sampling port (10).

6. A cellular bioreactor according to claim 1, wherein: the left side of the top end of the tank body (1) is provided with a dial (22).

7. A cellular bioreactor according to claim 1, wherein: the outer side of the roller (8) is provided with a polyurethane sleeve (23).