CN112779139B - Mesenchymal stem cell cryopreservation device and method - Google Patents
Mesenchymal stem cell cryopreservation device and method Download PDFInfo
- Publication number
- CN112779139B CN112779139B CN202011591760.6A CN202011591760A CN112779139B CN 112779139 B CN112779139 B CN 112779139B CN 202011591760 A CN202011591760 A CN 202011591760A CN 112779139 B CN112779139 B CN 112779139B
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- conveying
- sampling
- storage tank
- piston cover
- plate
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- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000005138 cryopreservation Methods 0.000 title claims description 15
- 238000005070 sampling Methods 0.000 claims abstract description 80
- 230000001105 regulatory effect Effects 0.000 claims description 13
- 238000007789 sealing Methods 0.000 claims description 12
- 210000000130 stem cell Anatomy 0.000 abstract description 20
- 230000005540 biological transmission Effects 0.000 abstract description 9
- 238000010257 thawing Methods 0.000 abstract description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 11
- 235000017491 Bambusa tulda Nutrition 0.000 description 11
- 241001330002 Bambuseae Species 0.000 description 11
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 11
- 239000011425 bamboo Substances 0.000 description 11
- 239000003814 drug Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
Abstract
The invention discloses a mesenchymal stem cell cryopreserving device and a cryopreserving method, wherein a sampling tube is stretched into a thawing cabin, a connecting body of a first sampling tube and a second sampling tube is rotated, so that the first sampling tube is communicated with a transmission hole on the surface of a conveying plate, the first piston cover is pushed and pressed, and then, the stem cells of a first part are collected, after the collection is completed, the sampling tube is not required to be taken out, the connecting body of the first sampling tube and the second sampling tube is rotated, the second sampling tube is communicated with the transmission hole of the conveying plate, and a sampling piston rod is pulled through sampling from a sampling hole, so that the stem cells are input into the sampling tube through the transmission hole and the sampling tube, and the sampling tube is not required to be pulled out when the two sections of stem cells are respectively sampled, thereby avoiding the external environment pollution of a culture dish in the operation process.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a mesenchymal stem cell cryopreservation device and a cryopreservation method.
Background
At present, after stem cells are separated and cultured from different human tissues, the stem cells are detected and identified, and then the stem cells are frozen in the deep low temperature of-196 ℃ so as to be convenient for resuscitating the stem cells for reinfusion of patients when clinically needed, thereby achieving the aim of treating diseases;
the existing culture dish cultures the stem cells after thawing, takes a sample to the stem cells through the sampling tube, however sometimes needs multiple times of sampling, and freezes respectively, and current sampling device carries out single sample to the stem cells, so after single sample freezes, need stretch into the culture dish again with sampling device and take a sample, so reciprocally, can cause outside air to enter into the contaminated culture solution in the culture dish.
Disclosure of Invention
The invention aims to provide a mesenchymal stem cell cryopreservation device and a cryopreservation method, and aims to solve the technical problem that in the prior art, a sampling device is required to extend into a culture dish again for sampling after single sampling and cryopreservation, so that external air can enter the culture dish to pollute culture solution.
In order to achieve the aim, the mesenchymal stem cell cryopreservation device comprises a storage tank, a sampling tube and a processing device; the storage tank is provided with a temporary storage cavity, the temporary storage cavity is positioned in the storage tank, the sampling tube is fixedly connected with the storage tank, is positioned on one side of the storage tank away from the temporary storage cavity, and is communicated with the temporary storage cavity; the processing device comprises a first collecting cylinder, a second collecting cylinder, a conveying plate and an adjusting component, wherein the first collecting cylinder is rotationally connected with the storage tank and is positioned in the temporary storage cavity, the second collecting cylinder is fixedly connected with the first collecting cylinder, is rotationally connected with the storage tank and is positioned at one side of the storage tank, which is close to the first collecting cylinder, the conveying plate is fixedly connected with the storage tank, is rotationally connected with the first collecting cylinder and is rotationally connected with the second collecting cylinder, the conveying plate is fixedly connected with the sampling cylinder and is positioned at one side of the storage tank, which is close to the sampling cylinder, and the conveying plate is provided with a conveying hole, which is positioned at one side of the conveying plate, which is close to the sampling cylinder, penetrates through the conveying plate and is communicated with the temporary storage cavity; the adjusting component comprises a first piston cover, a second piston cover and a collecting piston rod, wherein the first piston cover is in sliding connection with the first collecting cylinder and is positioned on one side of the first collecting cylinder, which is far away from the conveying plate, the second piston cover is in sliding connection with the second collecting cylinder and is positioned on one side of the second collecting cylinder, which is far away from the conveying plate, the second piston cover is provided with a sampling port, the sampling port penetrates through the second piston cover and is communicated with the temporary storage cavity, and the collecting piston rod is detachably connected with the second piston cover and is positioned on one side of the second piston cover, which is close to the sampling port and is closed with the sampling port.
The adjusting assembly further comprises a collection sealing ring, wherein the collection sealing ring is fixedly connected with the second piston cover, is abutted to the collection piston rod, and is located between the second piston cover and the collection piston rod.
The adjusting assembly further comprises a conveying piston rod, wherein the conveying piston rod is fixedly connected with the first piston cover and is located at one side, far away from the conveying plate, of the first piston cover.
The adjusting assembly further comprises a conveying guide pipe, wherein the conveying guide pipe is fixedly connected with the first piston cover, penetrates through the first piston cover and is located at one side, close to the conveying piston rod, of the first piston cover.
The adjusting assembly further comprises a flow speed adjusting plate and an adjusting roller, wherein the flow speed adjusting plate is fixedly connected with the conveying guide pipe and is positioned at one end of the conveying guide pipe far away from the first piston cover; the regulating roller is in sliding connection with the flow speed regulating plate, is abutted with the conveying guide pipe, and is positioned on one side of the flow speed regulating plate, which is close to the conveying guide pipe.
The treatment device further comprises a culture box body which is fixedly connected with the conveying guide pipe and is positioned at one end of the conveying guide pipe, which is close to the flow speed adjusting plate.
The processing device further comprises a conveying sealing ring, wherein the conveying sealing ring is fixedly connected with the conveying plate, detachably connected with the sampling tube and located on one side, close to the sampling tube, of the conveying plate.
According to the mesenchymal stem cell cryopreserving device and the cryopreserving method, the sampling tube stretches into the thawing cabin, the connecting bodies of the first sampling tube and the second sampling tube are rotated, so that the first sampling tube is communicated with the transmission hole on the surface of the conveying plate, the first piston cover is pushed and pressed, the first part of stem cells are further collected, after the collection is completed, the sampling tube is not required to be taken out, the connecting bodies of the first sampling tube and the second sampling tube are rotated, the second sampling tube is communicated with the transmission hole of the conveying plate, the sampling piston rod is pulled through sampling from the sampling hole, and therefore the stem cells are input into the sampling tube through the transmission hole and the sampling tube, and when the two sections of stem cells are respectively sampled, the sampling tube is not required to be pulled out, so that the culture dish is prevented from being polluted by external environment in the operation process.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic view of the structure of the treatment apparatus of the present invention.
Fig. 2 is a schematic diagram of the connection structure of the second collecting cylinder and the conveying plate of the present invention.
FIG. 3 is a schematic view showing the connection structure of the first collecting cylinder and the culture cassette of the present invention.
In the figure: 1-storage tank, 2-sampling tube, 3-processing apparatus, 11-temporary storage cavity, 31-first collection tube, 32-second collection tube, 33-delivery plate, 34-adjusting component, 35-culture box, 36-delivery sealing ring, 100-lavage device with medicine injection function, 331-transmission hole, 341-first piston cap, 342-second piston cap, 343-collection piston rod, 344-collection sealing ring, 345-delivery piston rod, 346-delivery conduit, 347-flow rate adjusting plate, 348-adjusting roller, 3421-sampling port.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present invention and should not be construed as limiting the invention.
In the description of the present invention, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present invention and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention. Furthermore, in the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Referring to fig. 1 to 2, the present invention provides a mesenchymal stem cell cryopreservation apparatus 100, comprising a storage tank 1, a sampling tube 2 and a processing device 3; the storage tank 1 is provided with a temporary storage cavity 11, the temporary storage cavity 11 is positioned in the storage tank 1, the sampling tube 2 is fixedly connected with the storage tank 1, is positioned on one side of the storage tank 1 far away from the temporary storage cavity 11, and is communicated with the temporary storage cavity 11; the processing device 3 comprises a first collecting cylinder 31, a second collecting cylinder 32, a conveying plate 33 and an adjusting component 34, wherein the first collecting cylinder 31 is rotationally connected with the storage tank 1 and is positioned in the temporary storage cavity 11, the second collecting cylinder 32 is fixedly connected with the first collecting cylinder 31 and is rotationally connected with the storage tank 1 and is positioned at one side of the storage tank 1 close to the first collecting cylinder 31, the conveying plate 33 is fixedly connected with the storage tank 1 and is rotationally connected with the first collecting cylinder 31 and is rotationally connected with the second collecting cylinder 32, the conveying plate 33 is fixedly connected with the sampling cylinder 2 and is positioned at one side of the storage tank 1 close to the sampling cylinder 2, the conveying plate 33 is provided with a conveying hole 331, and the conveying hole 331 is positioned at one side of the conveying plate 33 close to the sampling cylinder 2 and penetrates through the conveying plate 33 and is communicated with the temporary storage cavity 11; the adjusting assembly 34 includes a first piston cap 341, a second piston cap 342, and a collecting piston rod 343, where the first piston cap 341 is slidably connected with the first collecting cylinder 31 and is located on a side of the first collecting cylinder 31 away from the conveying plate 33, the second piston cap 342 is slidably connected with the second collecting cylinder 32 and is located on a side of the second collecting cylinder 32 away from the conveying plate 33, the second piston cap 342 has a sampling port 3421, the sampling port 3421 penetrates the second piston cap 342 and penetrates the temporary storage cavity 11, and the collecting piston rod 343 is detachably connected with the second piston cap 342 and is located on a side of the second piston cap 342 close to the sampling port 3421 and is covered with the sampling port 3421.
In this embodiment, the storage tank 1 is a cylinder, the temporary storage cavity 11 is formed in the storage tank 1, the temporary storage cavity 11 penetrates through the storage tank 1, the storage tank 1 is hollow, the conveying plate 33 is integrally fixed at the opening of the storage tank 1, one end opening of the storage tank 1 is sealed, the conveying plate 33 is provided with the conveying hole 331 on the surface, the sampling tube 2 is clamped on the conveying plate 33 and penetrates through the conveying hole 331 of the conveying plate 33, the temporary storage cavity 11 in the storage tank 1 is penetrated through, the first collecting tube 31 and the second collecting tube 32 are all semicircular, the first collecting tube 31 and the second collecting tube 32 are integrally fixed into a cylinder, the first collecting tube 31 and the second collecting tube 32 are separated on the diameter of the cylinder, the first collecting tube 31 and the second collecting tube 32 are respectively used for carrying out medicine feeding, the first collecting tube 31 and the second collecting tube 32 are respectively clamped on the conveying plate 33 and penetrate through the conveying hole 331, the first collecting tube 31 and the second collecting tube 32 are communicated through the conveying tube 33, and the first collecting tube 31 and the second collecting tube 32 are sequentially communicated with the conveying tube 32, and the conveying tube 32 are communicated through the conveying tube 31 and the conveying tube 32; the side of the first collection tube 31 far from the conveying plate 33 is sealed by the first piston cap 341, and stem cells in the first collection tube 31 are output through the conveying hole 331 and the sampling tube 2 by sliding the first piston cap 341 and the first collection tube 31; the second collection tube 32 is sealed by the second piston cap 342, and has the sampling port 3421 on the surface of the second piston cap 342, the sampling port 3421 is communicated with the second piston cap 342, so that medicine can be fed from the sampling port 3421, and the collection piston rod 343 can seal the sampling port 3421 and is fixedly connected with the second piston cap 342, so as to drive the second piston cap 342 to slide; so, will sample section of thick bamboo 2 stretches into the cabin that unfreezes, rotates first collection section of thick bamboo 31 with the connector of second collection section of thick bamboo 32 makes first collection section of thick bamboo 31 with the transmission hole 331 of delivery plate 33 surface link up, through bulldozing first piston lid 341, and then gather the stem cell of first part, after gathering, need not to take out sample section of thick bamboo 2, rotate first collection section of thick bamboo 31 with the connector of second collection section of thick bamboo 32 makes second collection section of thick bamboo 32 with the transmission hole 331 of delivery plate 33 link up, through follow sample mouth 3421, pulling collection piston rod 343 to pass through transmission hole 331 with sample section of thick bamboo 2 is input to in the sample section of thick bamboo, so when carrying out the sample respectively to two sections of stem cells respectively, need not to extract sample section of thick bamboo 2, thereby avoided the outside environmental pollution culture dish in the operation process.
Further, referring to fig. 1, the adjusting assembly 34 further includes a collecting seal ring 344, wherein the collecting seal ring 344 is fixedly connected with the second piston cap 342, abuts against the collecting piston rod 343, and is located between the second piston cap 342 and the collecting piston rod 343.
In this embodiment, the collecting seal ring 344 is integrally fixed to the sampling port 3421 of the second piston cap 342, and seals the collecting piston rod 343, so that the inside of the second collecting cylinder 32 is in a sealed state.
Further, referring to fig. 1, the adjusting assembly 34 further includes a conveying piston rod 345, where the conveying piston rod 345 is fixedly connected to the first piston cap 341 and located on a side of the first piston cap 341 away from the conveying plate 33.
In this embodiment, the conveying piston rod 345 is screwed to the side of the first piston cap 341 away from the conveying plate 33 and protrudes out of the storage tank 1, and the stem cells in the first collecting cylinder 31 are driven by pulling the conveying piston rod 345.
Further, referring to fig. 1 and 3, the adjusting assembly 34 further includes a delivery conduit 346, wherein the delivery conduit 346 is fixedly connected with the first piston cap 341 and penetrates the first piston cap 341, and is located on a side of the first piston cap 341 close to the delivery piston rod 345.
Further, referring to fig. 3, the adjusting assembly 34 further includes a flow rate adjusting plate 347 and an adjusting roller 348, wherein the flow rate adjusting plate 347 is fixedly connected to the conveying conduit 346 and is located at an end of the conveying conduit 346 away from the first piston cap 341; the regulating roller 348 is slidably connected to the flow rate regulating plate 347, abuts the conveying duct 346, and is located on a side of the flow rate regulating plate 347 close to the conveying duct 346.
Further, referring to FIG. 3, the treatment apparatus 3 further includes a culture box 35, wherein the culture box 35 is fixedly connected to the delivery conduit 346 and is located at an end of the delivery conduit 346 near the flow rate adjusting plate 347.
In this embodiment, one end of the delivery conduit 346 penetrates the first piston cap 341, and the other end is communicated with the culture box 35, so that the culture box 35 is communicated with the inside of the first collecting cylinder 31, and stem cells can be pumped into the first collecting cylinder 31, and stem cells in the first collecting cylinder 31 can be exported, and in the exporting process, the collection speed of the delivery conduit 346 is regulated by the flow rate regulating plate 347 and the regulating roller 348 located at the periphery of the delivery conduit 346, so that the output and input of stem cells in the delivery conduit 346 are regulated.
Further, referring to fig. 3, the processing apparatus 3 further includes a conveying sealing ring 36, where the conveying sealing ring 36 is fixedly connected to the conveying plate 33, detachably connected to the sampling tube 2, and located on a side of the conveying plate 33 near the sampling tube 2.
In this embodiment, the conveying seal ring 36 is integrally fixed on one side of the conveying plate 33 near the sampling tube 2, and clamps and limits the end of the sampling tube 2, so that the connectivity between the sampling tube 2 and the conveying plate 33 is better.
The above disclosure is only a preferred embodiment of the present invention, and it should be understood that the scope of the invention is not limited thereto, and those skilled in the art will appreciate that all or part of the procedures described above can be performed according to the equivalent changes of the claims, and still fall within the scope of the present invention.
Claims (7)
1. The mesenchymal stem cell cryopreservation device is characterized by comprising a storage tank, a sampling tube and a processing device;
the storage tank is provided with a temporary storage cavity, the temporary storage cavity is positioned in the storage tank, the sampling tube is fixedly connected with the storage tank, is positioned on one side of the storage tank away from the temporary storage cavity, and is communicated with the temporary storage cavity;
the processing device comprises a first collecting cylinder, a second collecting cylinder, a conveying plate and an adjusting component, wherein the first collecting cylinder is rotationally connected with the storage tank and is positioned in the temporary storage cavity, the second collecting cylinder is fixedly connected with the first collecting cylinder, is rotationally connected with the storage tank and is positioned at one side of the storage tank, which is close to the first collecting cylinder, the conveying plate is fixedly connected with the storage tank, is rotationally connected with the first collecting cylinder and is rotationally connected with the second collecting cylinder, the conveying plate is fixedly connected with the sampling cylinder and is positioned at one side of the storage tank, which is close to the sampling cylinder, and the conveying plate is provided with a conveying hole, which is positioned at one side of the conveying plate, which is close to the sampling cylinder, penetrates through the conveying plate and is communicated with the temporary storage cavity;
the adjusting component comprises a first piston cover, a second piston cover and a collecting piston rod, wherein the first piston cover is in sliding connection with the first collecting cylinder and is positioned on one side of the first collecting cylinder, which is far away from the conveying plate, the second piston cover is in sliding connection with the second collecting cylinder and is positioned on one side of the second collecting cylinder, which is far away from the conveying plate, the second piston cover is provided with a sampling port, the sampling port penetrates through the second piston cover and is communicated with the temporary storage cavity, and the collecting piston rod is detachably connected with the second piston cover and is positioned on one side of the second piston cover, which is close to the sampling port and is closed with the sampling port;
the adjusting assembly further comprises a conveying piston rod fixedly connected with the first piston cover and located on one side, away from the conveying plate, of the first piston cover.
2. A mesenchymal stem cell cryopreservation apparatus as claimed in claim 1, wherein,
the adjusting component further comprises a collection sealing ring, wherein the collection sealing ring is fixedly connected with the second piston cover, is abutted to the collection piston rod, and is positioned between the second piston cover and the collection piston rod.
3. A mesenchymal stem cell cryopreservation apparatus as claimed in claim 1, wherein,
the adjusting assembly further comprises a conveying guide pipe, wherein the conveying guide pipe is fixedly connected with the first piston cover, penetrates through the first piston cover and is positioned on one side, close to the conveying piston rod, of the first piston cover.
4. A mesenchymal stem cell cryopreservation apparatus as claimed in claim 3, wherein,
the adjusting assembly further comprises a flow speed adjusting plate and an adjusting roller, wherein the flow speed adjusting plate is fixedly connected with the conveying guide pipe and is positioned at one end of the conveying guide pipe far away from the first piston cover; the regulating roller is in sliding connection with the flow speed regulating plate, is abutted with the conveying guide pipe, and is positioned on one side of the flow speed regulating plate, which is close to the conveying guide pipe.
5. The mesenchymal stem cell cryopreservation apparatus of claim 4,
the treatment device further comprises a culture box body which is fixedly connected with the conveying guide pipe and is positioned at one end of the conveying guide pipe, which is close to the flow speed adjusting plate.
6. A mesenchymal stem cell cryopreservation apparatus as claimed in claim 1, wherein,
the processing device further comprises a conveying sealing ring, wherein the conveying sealing ring is fixedly connected with the conveying plate, detachably connected with the sampling tube and positioned on one side, close to the sampling tube, of the conveying plate.
7. A method for cryopreserving mesenchymal stem cells, which is used for solving the problem of collecting the mesenchymal stem cells according to claims 1-6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011591760.6A CN112779139B (en) | 2020-12-29 | 2020-12-29 | Mesenchymal stem cell cryopreservation device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011591760.6A CN112779139B (en) | 2020-12-29 | 2020-12-29 | Mesenchymal stem cell cryopreservation device and method |
Publications (2)
| Publication Number | Publication Date |
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| CN112779139A CN112779139A (en) | 2021-05-11 |
| CN112779139B true CN112779139B (en) | 2024-02-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202011591760.6A Active CN112779139B (en) | 2020-12-29 | 2020-12-29 | Mesenchymal stem cell cryopreservation device and method |
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| CN (1) | CN112779139B (en) |
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| CN112779139A (en) | 2021-05-11 |
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