CN112779139A - Mesenchymal stem cell cryopreservation device and cryopreservation method - Google Patents
Mesenchymal stem cell cryopreservation device and cryopreservation method Download PDFInfo
- Publication number
- CN112779139A CN112779139A CN202011591760.6A CN202011591760A CN112779139A CN 112779139 A CN112779139 A CN 112779139A CN 202011591760 A CN202011591760 A CN 202011591760A CN 112779139 A CN112779139 A CN 112779139A
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- 238000005138 cryopreservation Methods 0.000 title claims abstract description 25
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 10
- 238000005070 sampling Methods 0.000 claims abstract description 82
- 210000000130 stem cell Anatomy 0.000 claims abstract description 22
- 238000007789 sealing Methods 0.000 claims description 15
- 230000005540 biological transmission Effects 0.000 abstract description 11
- 238000010257 thawing Methods 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
Abstract
The invention discloses a mesenchymal stem cell cryopreservation device and a mesenchymal stem cell cryopreservation method, wherein a sampling cylinder is extended into a thawing cabin, a connecting body of a first collecting cylinder and a second collecting cylinder is rotated to ensure that the first collecting cylinder is communicated with a transmission hole on the surface of a conveying plate, and a first piston cover is pushed and pressed, further collecting the first part of stem cells, after the collection is finished, without taking out the sampling cylinder, rotating a connecting body of the first collection cylinder and the second collection cylinder to enable the second collection cylinder to be communicated with the transmission hole of the conveying plate, sampling from the sampling port, pulling the collection piston rod, so that the stem cells are input into the sampling cylinder through the transmission hole and the sampling cylinder, when two sections of stem cells are respectively sampled, the sampling cylinder is not required to be pulled out, so that the culture dish is prevented from being polluted by external environment in the operation process.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a mesenchymal stem cell cryopreservation device and a cryopreservation method.
Background
At present, stem cells are separated and cultured from different human tissues, and then are frozen at a deep low temperature of-196 ℃ after detection and identification, so that the stem cells can be revived for reinfusion to patients when clinically needed, and the purpose of treating diseases is achieved;
present culture dish cultivates the stem cell after unfreezing, takes a sample to the stem cell through the sampling tube, nevertheless needs sample many times sometimes to freeze and deposit respectively, and current sampling device carries out the single sample to the stem cell, so freeze and deposit the back at the single sample, need stretch into the culture dish with sampling device once more and take a sample, so reciprocal, can cause the outside air to enter into the culture dish in the pollution culture solution.
Disclosure of Invention
The invention aims to provide a mesenchymal stem cell cryopreservation device and a mesenchymal stem cell cryopreservation method, and aims to solve the technical problem that in the prior art, a sampling device is used for sampling stem cells once, so that after single sampling cryopreservation, the sampling device needs to be stretched into a culture dish again for sampling, and the reciprocating operation causes that external air enters the culture dish to pollute culture solution.
In order to achieve the purpose, the mesenchymal stem cell cryopreservation device comprises a storage tank, a sampling cylinder and a processing device; the sampling device comprises a storage tank, a sampling barrel, a sampling device and a sampling device, wherein the storage tank is provided with a temporary storage cavity, the temporary storage cavity is positioned inside the storage tank, the sampling barrel is fixedly connected with the storage tank, is positioned on one side of the storage tank, which is far away from the temporary storage cavity, and is communicated with the temporary storage cavity; the processing device comprises a first collecting barrel, a second collecting barrel, a conveying plate and an adjusting assembly, wherein the first collecting barrel is rotatably connected with the storage tank and is positioned inside the temporary storage cavity, the second collecting barrel is fixedly connected with the first collecting barrel and is rotatably connected with the storage tank and is positioned on one side of the storage tank close to the first collecting barrel, the conveying plate is fixedly connected with the storage tank and is rotatably connected with the first collecting barrel and is rotatably connected with the second collecting barrel, the conveying plate is fixedly connected with the sampling barrel and is positioned on one side of the storage tank close to the sampling barrel, the conveying plate is provided with a conveying hole, and the conveying hole is positioned on one side of the conveying plate close to the sampling barrel, penetrates through the conveying plate and is communicated with the temporary storage cavity; the adjusting component comprises a first piston cover, a second piston cover and a collecting piston rod, the first piston cover is in sliding connection with the first collecting cylinder and is located at one side of the conveying plate, the second piston cover is in sliding connection with the second collecting cylinder and is located at one side of the conveying plate, the second piston cover is provided with a sampling port, the sampling port penetrates through the second piston cover and is communicated with the temporary storage cavity, the collecting piston rod is detachably connected with the second piston cover and is located at one side of the sampling port, and the second piston cover is close to the sampling port.
The adjusting assembly further comprises a collecting sealing ring, the collecting sealing ring is fixedly connected with the second piston cover, is abutted against the collecting piston rod and is located between the second piston cover and the collecting piston rod.
The adjusting assembly further comprises a conveying piston rod, and the conveying piston rod is fixedly connected with the first piston cover and is located on one side, far away from the conveying plate, of the first piston cover.
The adjusting assembly further comprises a conveying conduit, wherein the conveying conduit is fixedly connected with the first piston cover, penetrates through the first piston cover and is positioned on one side, close to the conveying piston rod, of the first piston cover.
The adjusting assembly further comprises a flow rate adjusting plate and an adjusting roller, the flow rate adjusting plate is fixedly connected with the conveying conduit and is positioned at one end, far away from the first piston cover, of the conveying conduit; the adjusting roller is connected with the flow velocity adjusting plate in a sliding mode, is abutted to the conveying conduit and is located on one side, close to the conveying conduit, of the flow velocity adjusting plate.
Wherein, processing apparatus still includes cultivates the box body, cultivate the box body with conveying pipe fixed connection, and be located conveying pipe is close to the one end of velocity of flow regulating plate.
The processing device further comprises a conveying sealing ring, wherein the conveying sealing ring is fixedly connected with the conveying plate, detachably connected with the sampling cylinder and positioned on one side, close to the sampling cylinder, of the conveying plate.
The mesenchymal stem cell cryopreservation device and the mesenchymal stem cell cryopreservation method extend the sampling cylinder into a thawing cabin, rotate the connecting body of the first collecting cylinder and the second collecting cylinder to ensure that the first collecting cylinder is communicated with the transmission hole on the surface of the conveying plate, and push and press the first piston cover, further collecting the first part of stem cells, after the collection is finished, without taking out the sampling cylinder, rotating a connecting body of the first collection cylinder and the second collection cylinder to enable the second collection cylinder to be communicated with the transmission hole of the conveying plate, sampling from the sampling port, pulling the collection piston rod, so that the stem cells are input into the sampling cylinder through the transmission hole and the sampling cylinder, when two sections of stem cells are respectively sampled, the sampling cylinder is not required to be pulled out, so that the culture dish is prevented from being polluted by external environment in the operation process.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of the structure of a processing apparatus of the present invention.
Fig. 2 is a schematic view of a connection structure of a second collecting cylinder and a transfer plate of the present invention.
FIG. 3 is a schematic view showing a connection structure of a first collecting cylinder and a culture box body according to the present invention.
In the figure: 1-storage tank, 2-sampling tube, 3-processing device, 11-temporary storage cavity, 31-first collecting tube, 32-second collecting tube, 33-conveying plate, 34-regulating component, 35-culture box body, 36-conveying sealing ring, 100-lavaging device with drug injection function, 331-conveying hole, 341-first piston cover, 342-second piston cover, 343-collecting piston rod, 344-collecting sealing ring, 345-conveying piston rod, 346-conveying conduit, 347-flow rate regulating plate, 348-regulating roller and 3421-sampling port.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 to 2, the present invention provides a mesenchymal stem cell cryopreservation apparatus 100, including a storage tank 1, a sampling tube 2 and a processing apparatus 3; the storage tank 1 is provided with a temporary storage cavity 11, the temporary storage cavity 11 is positioned inside the storage tank 1, and the sampling tube 2 is fixedly connected with the storage tank 1, positioned on one side of the storage tank 1 far away from the temporary storage cavity 11 and communicated with the temporary storage cavity 11; the processing device 3 comprises a first collecting barrel 31, a second collecting barrel 32, a conveying plate 33 and an adjusting component 34, wherein the first collecting barrel 31 is rotatably connected with the storage tank 1 and is located in the temporary storage cavity 11, the second collecting barrel 32 is fixedly connected with the first collecting barrel 31 and is rotatably connected with the storage tank 1 and is located on one side of the storage tank 1 close to the first collecting barrel 31, the conveying plate 33 is fixedly connected with the storage tank 1 and is rotatably connected with the first collecting barrel 31 and is rotatably connected with the second collecting barrel 32, the conveying plate 33 is fixedly connected with the sampling barrel 2 and is located on one side of the storage tank 1 close to the sampling barrel 2, the conveying plate 33 is provided with a conveying hole 331, the conveying hole 331 is located on one side of the conveying plate 33 close to the sampling barrel 2 and penetrates through the conveying plate 33, and is communicated with the temporary storage cavity 11; the adjusting assembly 34 includes a first piston cap 341, a second piston cap 342, and a collecting piston rod 343, the first piston cap 341 is slidably connected to the first collecting cylinder 31 and located on a side of the first collecting cylinder 31 away from the conveying plate 33, the second piston cap 342 is slidably connected to the second collecting cylinder 32 and located on a side of the second collecting cylinder 32 away from the conveying plate 33, the second piston cap 342 has a sampling port 3421, the sampling port 3421 penetrates through the second piston cap 342 and is communicated with the temporary storage cavity 11, and the collecting piston rod 343 is detachably connected to the second piston cap 342 and located on a side of the second piston cap 342 close to the sampling port 3421 and is covered with the sampling port 3421.
In this embodiment, the storage tank 1 is a cylinder, the temporary storage cavity 11 is provided inside the storage tank 1, the temporary storage cavity 11 penetrates through the storage tank 1, so that the interior of the storage tank 1 is hollow, the transportation plate 33 is integrally fixed at the opening of the storage tank 1, one end opening of the storage tank 1 is sealed, the transportation plate 33 is provided with the transportation hole 331 on the surface thereof, the sampling cylinder 2 is connected to the transportation plate 33 in a snap manner, is communicated with the transportation hole 331 of the transportation plate 33, and is communicated with the temporary storage cavity 11 inside the storage tank 1, the first collection cylinder 31 and the second collection cylinder 32 have the same shape and are semicircular, the first collection cylinder 31 and the second collection cylinder 32 are integrally fixed as a cylinder, and the first collection cylinder 31 and the second collection cylinder 32 are separated from each other on the diameter of the cylinder, the first collection cylinder 31 and the second collection cylinder 32 are respectively used for lavage and drug administration, the bottoms of the first collection cylinder 31 and the second collection cylinder 32 extend into the conveying plate 33 and are rotatably connected with the surface of the conveying plate 33, so that the first collection cylinder 31 and the second collection cylinder 32 are sequentially communicated with the transmission hole 331, and only one of the first collection cylinder 31 and the second collection cylinder 32 can be communicated with the transmission hole 331; the side of the first collection cylinder 31 away from the conveying plate 33 is sealed by the first piston cap 341, and the stem cells in the first collection cylinder 31 are output through the transmission hole 331 and the sampling cylinder 2 by the sliding of the first piston cap 341 and the first collection cylinder 31; the second collecting cylinder 32 is sealed by the second piston cap 342, and has the sampling port 3421 on the surface of the second piston cap 342, the sampling port 3421 penetrates the second piston cap 342 so that medicine can be taken in from the sampling port 3421, and the collecting piston rod 343 can seal the sampling port 3421 and is fixedly connected with the second piston cap 342 so as to drive the second piston cap 342 to slide; in this way, by inserting the sampling tube 2 into the thawing compartment, rotating the connection body of the first collection tube 31 and the second collection tube 32 to allow the first collection tube 31 to communicate with the transfer hole 331 in the surface of the transfer plate 33, and pressing the first piston cap 341, further collecting the first part of stem cells, after the collection is completed, without taking out the sampling tube 2, rotating the connecting body of the first collection tube 31 and the second collection tube 32 to make the second collection tube 32 communicate with the transmission hole 331 of the transmission plate 33, sampling from the sampling port 3421, pulling the collection piston rod 343, thereby inputting stem cells into the sampling cylinder through the transfer hole 331 and the sampling cylinder 2, therefore, when two sections of stem cells are respectively sampled, the sampling cylinder 2 does not need to be pulled out, and the culture dish is prevented from being polluted by external environment in the operation process.
Further, referring to fig. 1, the adjusting assembly 34 further includes a collecting sealing ring 344, and the collecting sealing ring 344 is fixedly connected to the second piston cap 342, abuts against the collecting piston rod 343, and is located between the second piston cap 342 and the collecting piston rod 343.
In this embodiment, the collection seal 344 is integrally fixed to the sampling port 3421 of the second piston cap 342 to seal the collection piston rod 343 and to seal the interior of the second collection tube 32.
Further, referring to fig. 1, the adjusting assembly 34 further includes a delivering piston rod 345, and the delivering piston rod 345 is fixedly connected to the first piston cap 341 and is located on a side of the first piston cap 341 away from the delivering plate 33.
In this embodiment, the delivery piston rod 345 is screwed on the side of the first piston cap 341 away from the delivery plate 33 and extends out of the storage tank 1, and the stem cells in the first collection cylinder 31 are driven by pulling the delivery piston rod 345.
Further, referring to fig. 1 and 3, the adjusting assembly 34 further includes a delivery conduit 346, wherein the delivery conduit 346 is fixedly connected to the first piston cap 341, penetrates through the first piston cap 341, and is located on a side of the first piston cap 341 close to the delivery piston rod 345.
Further, referring to fig. 3, the adjusting assembly 34 further includes a flow rate adjusting plate 347 and an adjusting roller 348, wherein the flow rate adjusting plate 347 is fixedly connected to the delivery conduit 346 and is located at an end of the delivery conduit 346 away from the first piston cap 341; the adjusting roller 348 is slidably connected to the flow rate adjusting plate 347, abuts against the conveying pipe 346, and is located on a side of the flow rate adjusting plate 347 adjacent to the conveying pipe 346.
Further, referring to fig. 3, the processing device 3 further includes a culture box body 35, and the culture box body 35 is fixedly connected to the delivery conduit 346 and is located at one end of the delivery conduit 346 close to the flow rate adjustment plate 347.
In this embodiment, one end of the transfer tube 346 penetrates the first plunger cap 341 and the other end thereof penetrates the culture box body 35, so that the culture box body 35 communicates with the inside of the first collection cylinder 31, thereby pumping the stem cells into the first collection cylinder 31, and discharging the stem cells in the first collection cylinder 31, and the flow rate adjustment plate 347 and the adjustment roller 348 located on the outer periphery of the transfer tube 346 adjust the collection speed of the transfer tube 346 during the discharge, thereby adjusting the output and input of the stem cells into the transfer tube 346.
Further, referring to fig. 3, the processing device 3 further includes a conveying sealing ring 36, wherein the conveying sealing ring 36 is fixedly connected to the conveying plate 33, detachably connected to the sampling tube 2, and located on a side of the conveying plate 33 close to the sampling tube 2.
In this embodiment, the transportation sealing ring 36 is integrally fixed to the transportation plate 33 on a side close to the sampling tube 2, and clamps and limits the end of the sampling tube 2, so that the sampling tube 2 and the transportation plate 33 are better connected.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (8)
1. A mesenchymal stem cell cryopreservation device is characterized by comprising a storage tank, a sampling cylinder and a processing device;
the sampling device comprises a storage tank, a sampling barrel, a sampling device and a sampling device, wherein the storage tank is provided with a temporary storage cavity, the temporary storage cavity is positioned inside the storage tank, the sampling barrel is fixedly connected with the storage tank, is positioned on one side of the storage tank, which is far away from the temporary storage cavity, and is communicated with the temporary storage cavity;
the processing device comprises a first collecting barrel, a second collecting barrel, a conveying plate and an adjusting assembly, wherein the first collecting barrel is rotatably connected with the storage tank and is positioned inside the temporary storage cavity, the second collecting barrel is fixedly connected with the first collecting barrel and is rotatably connected with the storage tank and is positioned on one side of the storage tank close to the first collecting barrel, the conveying plate is fixedly connected with the storage tank and is rotatably connected with the first collecting barrel and is rotatably connected with the second collecting barrel, the conveying plate is fixedly connected with the sampling barrel and is positioned on one side of the storage tank close to the sampling barrel, the conveying plate is provided with a conveying hole, and the conveying hole is positioned on one side of the conveying plate close to the sampling barrel, penetrates through the conveying plate and is communicated with the temporary storage cavity;
the adjusting component comprises a first piston cover, a second piston cover and a collecting piston rod, the first piston cover is in sliding connection with the first collecting cylinder and is located at one side of the conveying plate, the second piston cover is in sliding connection with the second collecting cylinder and is located at one side of the conveying plate, the second piston cover is provided with a sampling port, the sampling port penetrates through the second piston cover and is communicated with the temporary storage cavity, the collecting piston rod is detachably connected with the second piston cover and is located at one side of the sampling port, and the second piston cover is close to the sampling port.
2. The mesenchymal stem cell cryopreservation device of claim 1, wherein,
the adjusting component further comprises a collecting sealing ring, the collecting sealing ring is fixedly connected with the second piston cover, is abutted against the collecting piston rod and is located between the second piston cover and the collecting piston rod.
3. The mesenchymal stem cell cryopreservation device of claim 1, wherein,
the adjusting assembly further comprises a conveying piston rod, and the conveying piston rod is fixedly connected with the first piston cover and is located on one side, far away from the conveying plate, of the first piston cover.
4. The mesenchymal stem cell cryopreservation device of claim 3,
the adjusting assembly further comprises a conveying conduit, and the conveying conduit is fixedly connected with the first piston cover, penetrates through the first piston cover and is located on one side, close to the conveying piston rod, of the first piston cover.
5. The mesenchymal stem cell cryopreservation device of claim 4, wherein,
the adjusting assembly further comprises a flow rate adjusting plate and an adjusting roller, the flow rate adjusting plate is fixedly connected with the conveying conduit and is positioned at one end, far away from the first piston cover, of the conveying conduit; the adjusting roller is connected with the flow velocity adjusting plate in a sliding mode, is abutted to the conveying conduit and is located on one side, close to the conveying conduit, of the flow velocity adjusting plate.
6. The mesenchymal stem cell cryopreservation device of claim 5, wherein the mesenchymal stem cell cryopreservation device comprises a stem cell and a stem cell,
the processing device further comprises a culture box body, wherein the culture box body is fixedly connected with the conveying guide pipe and is positioned at one end, close to the flow velocity adjusting plate, of the conveying guide pipe.
7. The mesenchymal stem cell cryopreservation device of claim 1, wherein,
the processing device further comprises a conveying sealing ring, wherein the conveying sealing ring is fixedly connected with the conveying plate, detachably connected with the sampling cylinder and positioned on one side, close to the sampling cylinder, of the conveying plate.
8. A cryopreservation method of mesenchymal stem cells, which is used for solving the collection problem of cryopreservation of mesenchymal stem cells as claimed in claims 1-7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011591760.6A CN112779139B (en) | 2020-12-29 | 2020-12-29 | Mesenchymal stem cell cryopreservation device and method |
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| CN202011591760.6A CN112779139B (en) | 2020-12-29 | 2020-12-29 | Mesenchymal stem cell cryopreservation device and method |
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| CN112779139A true CN112779139A (en) | 2021-05-11 |
| CN112779139B CN112779139B (en) | 2024-02-13 |
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2700851A1 (en) * | 1993-01-26 | 1994-07-29 | Sgn Soc Gen Tech Nouvelle | Device and method for taking samples of a material. |
| WO1996017238A1 (en) * | 1994-12-01 | 1996-06-06 | Richard Perry Sheryll | Underwater sampling method and apparatus |
| US20060039833A1 (en) * | 2004-08-04 | 2006-02-23 | Yong Peter A | Biological specimen collection, transportation, and dispensing system |
| CN105695303A (en) * | 2016-03-21 | 2016-06-22 | 北京金诺美生物技术有限公司 | Sample reaction device and reaction method |
| US20190133508A1 (en) * | 2017-11-09 | 2019-05-09 | Chi Wai Yeung | Sealed blood collection device for clinical blood testing |
| CN209342448U (en) * | 2018-10-23 | 2019-09-03 | 董超凡 | Sampler is used in a kind of detection of liquid food |
| CN209727543U (en) * | 2019-04-17 | 2019-12-03 | 天津中电天仪科技股份有限公司 | A kind of sewage sampling device |
| CN110926879A (en) * | 2019-12-13 | 2020-03-27 | 湖州锦鸿环保工程有限公司 | Water quality stratified sampling device of adjustable water intaking interlamellar spacing |
| CN111117870A (en) * | 2020-02-10 | 2020-05-08 | 张娣 | Liver cancer cell collector |
| CN111337304A (en) * | 2018-12-18 | 2020-06-26 | 东营职业学院 | Portable intelligent measurement sampling device |
| CN211367566U (en) * | 2020-05-12 | 2020-08-28 | 海南济民博鳌国际医院有限公司 | Anti-pollution straw for cell culture |
| CN112006720A (en) * | 2020-07-10 | 2020-12-01 | 兰州大学第二医院 | Medical science inspection sampling device |
-
2020
- 2020-12-29 CN CN202011591760.6A patent/CN112779139B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2700851A1 (en) * | 1993-01-26 | 1994-07-29 | Sgn Soc Gen Tech Nouvelle | Device and method for taking samples of a material. |
| WO1996017238A1 (en) * | 1994-12-01 | 1996-06-06 | Richard Perry Sheryll | Underwater sampling method and apparatus |
| US20060039833A1 (en) * | 2004-08-04 | 2006-02-23 | Yong Peter A | Biological specimen collection, transportation, and dispensing system |
| CN105695303A (en) * | 2016-03-21 | 2016-06-22 | 北京金诺美生物技术有限公司 | Sample reaction device and reaction method |
| US20190133508A1 (en) * | 2017-11-09 | 2019-05-09 | Chi Wai Yeung | Sealed blood collection device for clinical blood testing |
| CN209342448U (en) * | 2018-10-23 | 2019-09-03 | 董超凡 | Sampler is used in a kind of detection of liquid food |
| CN111337304A (en) * | 2018-12-18 | 2020-06-26 | 东营职业学院 | Portable intelligent measurement sampling device |
| CN209727543U (en) * | 2019-04-17 | 2019-12-03 | 天津中电天仪科技股份有限公司 | A kind of sewage sampling device |
| CN110926879A (en) * | 2019-12-13 | 2020-03-27 | 湖州锦鸿环保工程有限公司 | Water quality stratified sampling device of adjustable water intaking interlamellar spacing |
| CN111117870A (en) * | 2020-02-10 | 2020-05-08 | 张娣 | Liver cancer cell collector |
| CN211367566U (en) * | 2020-05-12 | 2020-08-28 | 海南济民博鳌国际医院有限公司 | Anti-pollution straw for cell culture |
| CN112006720A (en) * | 2020-07-10 | 2020-12-01 | 兰州大学第二医院 | Medical science inspection sampling device |
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| CN112779139B (en) | 2024-02-13 |
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