CN210796487U - Lymphocyte chromosome culture tube - Google Patents
Lymphocyte chromosome culture tube Download PDFInfo
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- CN210796487U CN210796487U CN201920576195.2U CN201920576195U CN210796487U CN 210796487 U CN210796487 U CN 210796487U CN 201920576195 U CN201920576195 U CN 201920576195U CN 210796487 U CN210796487 U CN 210796487U
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- tube
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- culture tube
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- 210000000349 chromosome Anatomy 0.000 title claims abstract description 17
- 210000004698 lymphocyte Anatomy 0.000 title claims abstract description 15
- 210000004369 blood Anatomy 0.000 claims abstract description 14
- 239000008280 blood Substances 0.000 claims abstract description 14
- 238000012360 testing method Methods 0.000 claims abstract description 14
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 14
- 229920001971 elastomer Polymers 0.000 claims description 9
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 7
- 239000001569 carbon dioxide Substances 0.000 claims description 7
- 229920005549 butyl rubber Polymers 0.000 claims description 3
- 238000010241 blood sampling Methods 0.000 abstract description 10
- 230000006978 adaptation Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 9
- 230000010261 cell growth Effects 0.000 description 8
- 239000012980 RPMI-1640 medium Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 4
- 108010047620 Phytohemagglutinins Proteins 0.000 description 3
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- 238000012546 transfer Methods 0.000 description 3
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical class N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 230000001885 phytohemagglutinin Effects 0.000 description 2
- 229920001707 polybutylene terephthalate Polymers 0.000 description 2
- -1 polyethylene terephthalate Polymers 0.000 description 2
- 229920000139 polyethylene terephthalate Polymers 0.000 description 2
- 239000005020 polyethylene terephthalate Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003792 electrolyte Chemical class 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
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- 229920000669 heparin Polymers 0.000 description 1
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 description 1
- 229960001008 heparin sodium Drugs 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 229940116540 protein supplement Drugs 0.000 description 1
- 235000005974 protein supplement Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
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- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
The utility model belongs to a lymphocyte chromosome culture tube, including body, pipe cap, plug, the pipe cap connects with the body suitable for reading, is equipped with the puncture blood sampling mouth on the pipe cap, and inside the sealed puncture blood sampling mouth of plug was fixed in the pipe cap, a serial communication port, the body adopted the round bottom test tube, the round bottom structure and the centrifugal device jack looks adaptation of round bottom test tube, the inside inner chamber of body was equipped with the vacuum layer in advance, be equipped with the vacuum layer in advance in the vacuum layer in advance. The utility model discloses a culture tube convenient to use nature improvement: a certain amount of vacuum negative pressure is preset in each culture tube, and the blood sample is automatically sucked, so that the blood is sampled and cultured completely in a closed state, the pollution can be reduced, and the use convenience is also improved.
Description
[ technical field ]
The utility model belongs to the field of medical instrument makes, specific culture tube for chromosome culture that says so.
[ background art ]
Prior art assemblies for chromosome culture of lymphocytes typically include disposable syringes, petri dishes or flasks, and centrifuge tubes that must be used in later operations. After blood is collected by using a disposable syringe and then is injected into a culture dish (or a culture bottle) with culture solution to complete cell culture, the culture needs to be transferred into a centrifugal tube to complete separation operation by matching with a centrifugal machine. Because syringe, centrifuging tube and culture dish (blake bottle) are a plurality of parts of mutual independence, there is the pollution risk easily at the transfer in-process to cultivate the cell, and whole complex operation.
Chinese patent CN207031449U discloses an in vitro cell culture tube, some above-mentioned technical problems have been overcome, can carry out cell culture and can carry out centrifugal operation, need not liquid transfer, effectively avoid polluting among the cell chromosome transfer process and make mistakes, but its body bottom that discloses is toper sharp bottom structure, this sharp bottom structure makes the cell reduce the area of contact with the culture solution in the culture process and is unfavorable for the growth of lymphocyte, the cell stock is lower and with the incomplete adaptation of centrifugal equipment jack, because be a sealed container, the metabolite that cell growth process produced can change the pH valve of culture solution, it is unfavorable to cell growth. And can not automatic blood sampling, still need inject blood into centrifuging tube with the syringe, complex operation has the pollution risk.
[ summary of the invention ]
An object of the utility model is to overcome the defect that prior art exists, provide a multi-functional chromosome cell culture tube, have automatic blood sampling concurrently, provide the environment that cell growth needs, wait to cultivate cell and culture solution contact more abundant, basic functions such as lymphocyte chromosome harvest separation centrifugation.
In order to realize the purpose, a lymphocyte chromosome culture tube is designed, which comprises a tube body, a tube cap and a rubber plug, wherein the tube cap is matched and connected with the upper opening of the tube body, the tube cap is provided with a puncture blood sampling opening, the rubber plug seals the puncture blood sampling opening and is fixed inside the tube cap, the tube body adopts a round-bottom test tube, the round-bottom structure of the round-bottom test tube is matched with a jack of a centrifugal device, the contact area between cells to be cultured and a culture solution is increased compared with a pointed-bottom centrifugal tube, the test tube inside the tube body is preset with a certain negative pressure, the purpose of automatic blood sampling can be achieved by connecting the blood sampling needle with a human body vein, the volume of the culture solution is removed according to the volume calculation of the test tube before the internal pre-vacuum of the test tube, 5-10% of carbon dioxide gas is pre-filled, the change of the pH value of the culture, is very beneficial to the growth of cells.
The pipe cap adopts half a mouthful of style of calligraphy structure, and the inside cavity installs the plug additional.
The inner wall of the inner cavity of the pipe cap is provided with a limiting buckle.
The rubber plug adopts a butyl rubber plug, which is beneficial to ensuring the negative pressure in the pipe.
The tube body is made of glass or high polymer materials, such as polyethylene terephthalate (PET), polybutylene terephthalate (PBT), Polycarbonate (PC) and the like, as long as the materials for ensuring the vacuum in the tube are in an optional range, the length of the test tube is preferably 100mm-125mm, and the diameter of the test tube is preferably 10-18 mm. If the volume of the test tube is too small, the hypotonic and fixed treatment in the subsequent cell harvesting process is not facilitated.
The utility model discloses a culture tube convenient to use, the function is comparatively multiple. Specifically, the method comprises the following steps:
1. the bottom of the tube body is designed to be round bottom, the culture solution is fully contacted with the cells to be cultured, and the tube body can also be effectively matched with centrifugal equipment;
2. a certain amount of vacuum negative pressure is preset in the culture tube, the blood sample is automatically sucked, and the blood is completely in a closed state after the blood is sampled and cultured, so that the pollution can be reduced, and the use convenience is also improved;
3. the inside of the test tube is calculated according to the volume of the culture solution and the volume of the vacuum negative pressure, carbon dioxide with a certain concentration (5-10%) can be pre-filled, the cell growth environment is effectively improved, and the damage to the cell growth caused by the fact that metabolites change the pH value of the culture solution in the cell growth process is avoided.
[ description of the drawings ]
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a usage state diagram of the present invention.
The notation in the figure is:
the blood collection tube comprises a tube body 1, a tube cap 2, a rubber plug 3, a puncture blood collection port 4 and a pre-vacuum layer 5.
[ detailed description of the invention ]
The technical solution of the present invention will be further described with reference to the accompanying drawings and examples, which are believed to be clear to those skilled in the art.
Example one
As shown in figure 1, a lymphocyte chromosome culture tube comprises a tube body 1, a tube cap 2 and a rubber plug 3,
the tube body 1 adopts a round-bottom test tube, the round-bottom structure of the round-bottom test tube is matched with the jack of the centrifugal equipment, a pre-vacuum layer is arranged in an inner cavity of the tube body, carbon dioxide with a certain concentration is pre-filled in the tube body, the length of the tube body is 100mm-125mm, and the diameter of the tube body is 10-18 mm.
The tube cap 2 is matched and connected with the upper opening of the tube body 1, the tube cap 2 is provided with a puncture blood sampling opening 4, and the rubber plug 3 seals the puncture blood sampling opening and is fixed inside the tube cap 2.
Example two
When used, the tube 1 is filled with a culture medium, the culture medium used for cell chromosome culture is a classical RPMI1640 culture medium, and RPMI-1640 is developed by Moore et al, Rosellel Park Memori research institute, and is abbreviated as RPMI. The medium formulation was based on RPMI-1630 series medium, using bicarbonate buffer system, and the amino acids and vitamins were adjusted. RPMI-1640 medium has been used to culture human normal and neoplastic leukocytes. RMPI-1640, if supplemented appropriately, is widely applicable to the culture of a variety of cell types, including the culture of fresh human lymphocytes in 72 hour Phytohemagglutinin (PHA) stimulation experiments. RPMI1640 medium contains biotin, vitamins and electrolyte salts, furthermore vitamin inositol and choline are present in very high concentrations, but does not contain proteins, lipids and growth factors, RPMI1640 medium uses a sodium bicarbonate buffer system, therefore a 5-10% carbon dioxide environment is required to maintain physiological pH. The pre-vacuum layer can therefore be further charged with carbon dioxide in order to optimize the culture conditions.
The utility model discloses can select for use RPMI1640 basic culture solution, add a certain amount of anticoagulant (like heparin sodium), cell growth stimulant (phytohemagglutinin PHA), protein supplement (calf serum or fetal calf serum), antibiotic (like penicillin, streptomycin etc.) according to the cultivation usage.
When the device is used, a certain amount of vacuum negative pressure is preset in each culture tube, and a certain amount of carbon dioxide is filled in advance according to the using amount of the culture solution and the requirement of culturing cells, so that a blood sample can be automatically sucked, the blood is sampled until the culture is completely in a closed state, the pollution is reduced, a good cell culture environment can be provided, and the use convenience is also improved.
Claims (6)
1. The utility model provides a lymphocyte chromosome culture tube, includes body, tube cap, plug, and the tube cap connects with body suitable for reading, is equipped with the puncture blood taking port on the tube cap, and inside the sealed puncture blood taking port of plug was fixed in the tube cap, its characterized in that, the body adopts the round bottom test tube, is equipped with carbon dioxide in the cavity of body.
2. The chromosome culture tube for lymphocytes according to claim 1, wherein a vacuum is pre-evacuated in the hollow cavity of the tube body to generate a vacuum negative pressure in the culture tube for automatically aspirating a blood sample.
3. The lymphocyte chromosome culture tube according to claim 1, wherein the tube cap is in a half-square structure, and a rubber plug is additionally arranged in an inner cavity.
4. The chromosome culture tube for lymphocytes as in claim 2, wherein the inner wall of the inner cavity of the tube cap is provided with a limit buckle.
5. The chromosome culture tube for lymphocytes according to claim 1 or 2, wherein the rubber plug is butyl rubber plug.
6. A lymphocyte chromosome culture tube according to claim 1, wherein the length of the tube is preferably 100mm to 125mm, and the diameter of the tube is preferably 10 mm to 18 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920576195.2U CN210796487U (en) | 2019-04-25 | 2019-04-25 | Lymphocyte chromosome culture tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920576195.2U CN210796487U (en) | 2019-04-25 | 2019-04-25 | Lymphocyte chromosome culture tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210796487U true CN210796487U (en) | 2020-06-19 |
Family
ID=71247174
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920576195.2U Active CN210796487U (en) | 2019-04-25 | 2019-04-25 | Lymphocyte chromosome culture tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210796487U (en) |
-
2019
- 2019-04-25 CN CN201920576195.2U patent/CN210796487U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200826 Address after: 201609 Shanghai City, Songjiang District Road No. 59 leaf Yexie town Patentee after: LINGEN PRECISION MEDICAL PRODUCTS (SHANGHAI) Co.,Ltd. Address before: 230071 Room 1002, 7 Queyue Mansion, Huaining Road, Shushan District, Hefei City, Anhui Province Patentee before: Zhu Dexin |