CN208933384U - A kind of culture tube - Google Patents

A kind of culture tube Download PDF

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Publication number
CN208933384U
CN208933384U CN201821580046.5U CN201821580046U CN208933384U CN 208933384 U CN208933384 U CN 208933384U CN 201821580046 U CN201821580046 U CN 201821580046U CN 208933384 U CN208933384 U CN 208933384U
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CN
China
Prior art keywords
tube body
tube
culture
insertion part
outer cover
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Active
Application number
CN201821580046.5U
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Chinese (zh)
Inventor
杨致亭
姚立琼
潘德芹
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Kanghua Biomedical Technology Co., Ltd
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WEIFANG KANGHUA BIOTECH CO Ltd
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Priority to CN201821580046.5U priority Critical patent/CN208933384U/en
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Abstract

The utility model provides a kind of culture tube, including tube body, and the tube body is equipped with pipe lid, and the pipe lid includes inner cover and outer cover, and the outer cover is detachably connected with tube body, and the inner cover includes insertion part and limiting section, and the insertion part is for being embedded in tube body.With the above mentioned technical proposal, compared with prior art, the utility model has the advantages that: 1, good leak-proof effect, culture medium can be guaranteed from microbial contamination, it is omitted the step of being sealed after being loaded with sealed membrane, while two kinds of vaccination ways can also be met: being inoculated with in pipettor inoculation and laboratory with syringe in superclean bench;2, the storage, transport, fostering requirement for both having met culture medium also meet the requirement of culture medium centrifugation, hypotonic, fixed etc..Centrifuge tube need not be gone to from vial again, simplify experimental implementation, avoid operational pollution.

Description

A kind of culture tube
Technical field
The utility model relates to cell culture apparatus fields.
Specifically, being to be related to a kind of culture tube.
Background technique
The diagnosis of clinical chromosomal disorders needs first to cultivate the cells, then carries out relative chromosome preparation and analysis. Peripheral blood lymphocytes culture medium is a kind of culture medium for being specifically used to cultivate peripheral blood lymphocytes, clinically first with outer All blood lymphocyte culture mediums carry out in-vitro multiplication culture to lymphocyte.Then it carries out that cell is harvested by centrifugation, to the thin of harvest Born of the same parents carry out the processing such as hypotonic, fixed, dry, aging, enzymic digestion and aobvious band, carry out caryogram point to chromosome finally by microscopy Analysis, to judge whether there is chromosome deficiency, diagnoses defect that may be present.
Traditional peripheral blood lymphocytes culture medium generally carries out sterile sealing preservation, transport and culture with vial. When carrying out cell culture, the fresh whole blood of 0.5ml or so is added into vial with pipettor, is placed in 37 DEG C of water baths and cultivates 68-72h.2h is added colchicine and continues to cultivate before lymphocyte harvests, and cell culture terminates and colchicine processing is completed, By in vial whole culture mediums and cell be transferred in the conical centrifuge tube of 10ml.The preparation process of whole chromosome include from The heart, hypotonic, fixed etc. are carried out in centrifuge tube.Finally carry out drop piece, aging, dyeing, microscopy.
Peripheral blood lymphocytes is held with traditional vial, is saved, and culture has three aspects.Firstly, connecing The step of entering whole blood and colchicine must sterile working in superclean bench, it is more demanding to operating environment.Secondly, lymph Cell culture terminates, and culture medium and cell is needed when harvest to be all transferred to centrifuge tube, complicated for operation, is easy to pollute, Er Qielang Take consumptive material.Finally, need to be resealed with sealed membrane and be cultivated after glass culture bottle Kaifeng access whole blood or colchicine, It is cumbersome.
Occur the cell culture tube of individual improvement versions currently on the market, is suitable for full-automatic cell culture device, is training The top cover for supporting pipe is equipped with the well of diameter 2-3mm, realizes machine sample-adding.But some hospitals are still accustomed to grasping manually Make, and existing improvement version cell culture tube is not suitable for pipettor gun head and heals.
Utility model content
The purpose of the utility model is to overcome the shortcomings of above-mentioned traditional technology, provide a kind of culture tube, Ji Nengcheng The carrier of culture medium storage, transport is carried on a shoulder pole, and can be used for cell culture, while can be used as centrifuge tube again to collect cell.In this way Experimental implementation, and the pollution that can cause to avoid media transfer can not only be simplified.
The purpose of this utility model is reached by following technical measures:
A kind of culture tube, including tube body, the tube body are equipped with pipe lid, and the pipe lid includes inner cover and outer cover, described outer Lid is detachably connected with tube body, and the inner cover includes insertion part and limiting section, and the insertion part is for being embedded in tube body.
As an improvement: the insertion part is equipped with cavity far from the side of limiting section.
As an improvement: the outer diameter of the insertion part and the internal diameter of tube body are identical.
As an improvement: the outer diameter of the limiting section and the outer diameter of tube body are identical.
As an improvement: it covers outside described equipped with through-hole.
As an improvement: the outer cover is threadedly coupled with tube body, is equipped with internal screw thread on the inside of the outer cover, on the tube body End outside is equipped with external screw thread, and the insertion part is made of elastic material, and the outer cover is made of plastics.
As an improvement: the tube body is equipped with scale.
As an improvement: the tube body is equipped with mark zone.
As an improvement: the bottom of the pipe is equipped with tapered portion.
As an improvement: the bottom of the pipe is equipped with support portion, and the shape of the support portion is tubular, the support portion Circumferentially it is arranged along tapered portion.
By adopting the above-described technical solution, compared with prior art, the utility model has the advantages that:
1, good leak-proof effect, it is ensured that culture medium is sealed after being omitted sample-adding with sealed membrane from microbial contamination The step of, while two kinds of vaccination ways can also be met: syringe is used in pipettor inoculation and laboratory in superclean bench Inoculation;
2, the storage, transport, fostering requirement for both having met culture medium also meet culture medium centrifugation, hypotonic, fixed etc. It is required that.Centrifuge tube need not be gone to from vial again, simplify experimental implementation, avoid operational pollution.
The utility model is described in further detail with reference to the accompanying drawings and detailed description.
Detailed description of the invention
Attached drawing 1 is a kind of structural schematic diagram of culture tube of the utility model.
Attached drawing 2 is the structural schematic diagram of inner cover in a kind of culture tube of the utility model.
Attached drawing 3 is the structural schematic diagram of outer cover in a kind of culture tube of the utility model.
Attached drawing 4 is the structural schematic diagram of outer cover in a kind of culture tube of the utility model.
Attached drawing 5 is a kind of structural schematic diagram of culture tube middle tube body of the utility model.
In figure: 1- tapered portion;2- support portion;3- tube body;4- inner cover;5- outer cover;6- through-hole;7- cavity;8- insertion part;9- Limiting section;10- internal screw thread;11- external screw thread;The mark zone 12-;13- scale.
Specific embodiment
Embodiment: as shown in Fig. 1, a kind of culture tube, including tube body 3, the shape of the tube body 3 is cylindrical shape, described Tube body 3 is made of transparent plastic, and the tube body 3 is equipped with pipe lid, and the pipe lid includes inner cover 4 and outer cover 5.
As shown in Fig. 2, the inner cover 4 includes insertion part 8 and limiting section 9, and the shape of the insertion part 8 is cylinder, The length of the insertion part 8 is 5 to 10 millimeters, and the shape of the limiting section 9 is cylinder, and the outer diameter of the limiting section 9 is greater than The outer diameter of insertion part 8, the insertion part 8 and limiting section 9 use integrated design.
For the insertion part 8 for being embedded in inside tube body 3, the limiting section 9 is too deep for preventing insertion part 8 to be embedded in tube body 3.
Specifically, the insertion part 8 is made of elastic material, the elastic material is rubber, SBS or TPE.It is described embedded Portion 8 is equipped with cavity 7 far from the side of limiting section.The cavity 7 is located at 8 centre of insertion part, the shape in 7 section of cavity For circle.
The outer diameter of the insertion part 8 is identical as the internal diameter of tube body 3, the outer diameter phase of the outer diameter and tube body 3 of the limiting section 9 Together.What is sealed in this way is more preferable, it is ensured that culture medium is from pollution in tube body 3.
As shown in attached drawing 3,4, the outer cover 5 is cylindrical shape, and the outer cover 5 is detachably connected with tube body 3.
Specifically, the outer cover 5 is made of plastics.The outer cover 5 is threadedly coupled with tube body 3.It is equipped on the inside of the outer cover 5 Internal screw thread 10,3 upper end of the tube body outside are equipped with external screw thread 11 corresponding with internal screw thread 10.
The outer cover 5 is equipped with through-hole 6, and the through-hole 6 is located at 5 top center of outer cover, and the shape of the through-hole 6 is Circle, the diameter of the through-hole 6 are 2 to 10 millimeters.
This design had not only been able to satisfy pipettor inoculation but also had been able to satisfy and had been inoculated with syringe, used sealed membrane after being omitted inoculation The tedious steps of sealing.
As shown in Fig. 5,3 lower end of tube body is equipped with tapered portion 1.3 lower end of tube body is additionally provided with support portion 2, described The shape of support portion 2 is tubular, and the support portion 2 is along the circumferential setting of tapered portion 1.
Facilitate the placement of tube body 3, can directly be vertically disposed in experimental bench, it is not necessary to be placed on rack for test tube.
3 side of tube body is coated with scale 13, and the volume of the tube body 3 is 10 milliliters.
3 outer wall of tube body is sprayed with scale 13, after harvesting cell, facilitates control that the amount of hypotonic medium and fixer is added, directly logical Scaleover 13 controls dosage.
3 side of tube body is coated with frosted mark zone 12, and the frosted mark zone 12 is located at 13 back side of scale.
3 outer wall of tube body is sprayed with frosted mark zone 12, facilitates the label in experimentation to sample number.
The specifically used method of the utility model:
One, pipettor is inoculated with:
1, superclean bench, ultraviolet light irradiation 30min or so are opened;
2, the outer cover 5 of tube body 3 is unscrewed with thumb and index finger, then the insertion part 8 of inner cover 4 is removed, and is drawn with pipettor Fresh whole blood 0.5ml is added in tube body 3, jiggles mixing;
3, whole blood is injected, first buckles the insertion part 8 of 3 inner cover 4 of tube body, then screw outer cover 5;
4, culture tube is placed in 37 DEG C of water-baths and is cultivated.Operating method is identical when adding colchicine.
Two, syringe is inoculated with:
1, the asepsis injector of an appropriate range is taken, fresh whole blood 0.5ml is drawn;
2, from insertion needle from the through-hole 6 of 3 outer cover 5 of tube body, whole blood is injected;
3, injection whole blood extracts syringe needle, and culture tube is placed in 37 DEG C of water-baths and is cultivated.Operating method when adding colchicine It is identical.
The utility model is able to satisfy the requirement of two kinds of sample loading alternatives, and structure is simple, easy to use.
One embodiment of the utility model is described in detail above, but the content is only the utility model Preferred embodiment should not be considered as limiting the scope of the present invention.It is all to be made according to application scope of the utility model All the changes and improvements etc., should all belong within the patent covering scope of the utility model.

Claims (10)

1. a kind of culture tube, including tube body (3), it is characterised in that: the tube body (3) is equipped with pipe lid, and the pipe lid includes interior Lid (4) and outer cover (5), the outer cover (5) are detachably connected with tube body (3), and the inner cover (4) includes insertion part (8) and limit Portion (9), the insertion part (8) is for being embedded in tube body (3).
2. culture tube according to claim 1, it is characterised in that: the insertion part (8) is equipped with far from the side of limiting section Cavity (7).
3. culture tube according to claim 1, it is characterised in that: the outer diameter of the insertion part (8) and the internal diameter of tube body (3) It is identical.
4. culture tube according to claim 3, it is characterised in that: the outer diameter of the limiting section (9) and the outer diameter of tube body (3) It is identical.
5. culture tube according to claim 1, it is characterised in that: the outer cover (5) is equipped with through-hole (6).
6. culture tube according to claim 1, it is characterised in that: the outer cover (5) is threadedly coupled with tube body (3), described Internal screw thread (10) are equipped on the inside of outer cover (5), are equipped with external screw thread (11) on the outside of tube body (3) upper end, the insertion part (8) is by bullet Property material is made, and the outer cover (5) is made of plastics.
7. according to claim 1 to 6 one of them described culture tube, it is characterised in that: the tube body (3) is equipped with scale (13).
8. according to claim 1 to 6 one of them described culture tube, it is characterised in that: the tube body (3) is equipped with mark zone (12).
9. according to claim 1 to 6 one of them described culture tube, it is characterised in that: tube body (3) lower end is equipped with taper Portion (1).
10. culture tube according to claim 9, it is characterised in that: tube body (3) lower end is equipped with support portion (2), described The shape of support portion (2) is tubular, and the support portion (2) is circumferentially arranged along tapered portion (1).
CN201821580046.5U 2018-09-27 2018-09-27 A kind of culture tube Active CN208933384U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201821580046.5U CN208933384U (en) 2018-09-27 2018-09-27 A kind of culture tube

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201821580046.5U CN208933384U (en) 2018-09-27 2018-09-27 A kind of culture tube

Publications (1)

Publication Number Publication Date
CN208933384U true CN208933384U (en) 2019-06-04

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Family Applications (1)

Application Number Title Priority Date Filing Date
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Country Status (1)

Country Link
CN (1) CN208933384U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113061521A (en) * 2021-04-06 2021-07-02 英诺维尔智能科技(苏州)有限公司 High-effect cell culture tube
CN116875548A (en) * 2023-09-08 2023-10-13 山东康华生物医疗科技股份有限公司 NK cell activity culture tube and production process thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113061521A (en) * 2021-04-06 2021-07-02 英诺维尔智能科技(苏州)有限公司 High-effect cell culture tube
CN116875548A (en) * 2023-09-08 2023-10-13 山东康华生物医疗科技股份有限公司 NK cell activity culture tube and production process thereof
CN116875548B (en) * 2023-09-08 2024-01-02 山东康华生物医疗科技股份有限公司 NK cell activity culture tube and production process thereof

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Address after: No.699, Yuehe Road, Weifang Economic Development Zone, Weifang City, Shandong Province

Patentee after: Shandong Kanghua Biomedical Technology Co., Ltd

Address before: 261023 Shandong city of Weifang Province River Road Economic Development Zone No. 699

Patentee before: WEIFANG KANGHUA BIOTECH Co.,Ltd.

CP03 Change of name, title or address