CN219839713U - Integrated cell culture bottle for culturing and harvesting blood specimens - Google Patents
Integrated cell culture bottle for culturing and harvesting blood specimens Download PDFInfo
- Publication number
- CN219839713U CN219839713U CN202321191715.0U CN202321191715U CN219839713U CN 219839713 U CN219839713 U CN 219839713U CN 202321191715 U CN202321191715 U CN 202321191715U CN 219839713 U CN219839713 U CN 219839713U
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- Prior art keywords
- harvesting
- bottle
- culture bottle
- tube
- cell culture
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- 238000003306 harvesting Methods 0.000 title claims abstract description 85
- 210000004369 blood Anatomy 0.000 title claims abstract description 32
- 239000008280 blood Substances 0.000 title claims abstract description 32
- 238000004113 cell culture Methods 0.000 title claims abstract description 24
- 238000012258 culturing Methods 0.000 title claims abstract description 10
- 239000004033 plastic Substances 0.000 claims description 9
- 229920003023 plastic Polymers 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 239000006143 cell culture medium Substances 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 229920005644 polyethylene terephthalate glycol copolymer Polymers 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims 2
- 238000000034 method Methods 0.000 abstract description 9
- 210000004027 cell Anatomy 0.000 abstract description 5
- 238000011081 inoculation Methods 0.000 abstract description 4
- 238000004458 analytical method Methods 0.000 abstract description 2
- 210000004748 cultured cell Anatomy 0.000 abstract description 2
- 230000012010 growth Effects 0.000 abstract description 2
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 6
- 210000000349 chromosome Anatomy 0.000 description 6
- 210000005259 peripheral blood Anatomy 0.000 description 6
- 239000011886 peripheral blood Substances 0.000 description 6
- 230000010261 cell growth Effects 0.000 description 3
- 229960001338 colchicine Drugs 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000031864 metaphase Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- 208000011359 Chromosome disease Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 208000024971 chromosomal disease Diseases 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an integrated cell culture bottle for culturing and harvesting blood samples, which relates to the technical field of laboratory consumables and comprises a conical culture bottle and a harvesting tube, wherein the conical culture bottle comprises a bottle body and a bottle neck, a first internal thread is arranged on the inner wall of the bottle neck, the integrated cell culture bottle for culturing and harvesting the blood samples is positioned in the integrated culture bottle before harvesting by enabling an inoculation sample and a harvesting sample to be the same consumable product, the conical culture bottle and the harvesting tube are detachably connected through threads, and a transfer tube can drain cultured cells into the harvesting tube, so that a culture bottle cap does not need to be removed before transferring the samples, a culture bottle plug and a tube cap of a centrifuge tube are opened, the operation process is simplified, and sample scattering or sample confusion caused by sample transfer is avoided. The inoculated blood sample grows in a conical flask with a large space, so that cells are in a good growth state, and a qualified analysis sample is obtained in subsequent experimental operation.
Description
Technical Field
The utility model relates to the technical field of laboratory consumables, in particular to an integrated cell culture bottle for culturing and harvesting blood samples.
Background
Peripheral blood karyotyping is a gold standard for diagnosis of chromosomal disorders. The preparation cycle of peripheral blood chromosomes is long, the operation is complicated, and the preparation method comprises the processes of nuclear collection and inoculation of blood specimens, blood specimen culture, harvesting of peripheral blood lymphocytes and chromosome flaking. The specific operation is as follows: firstly, collecting the blood sampling tubes with peripheral blood samples of patients one by one, sucking out 0.5ml of the peripheral blood samples by using an injector, injecting the peripheral blood samples into a cell culture flask, mixing the peripheral blood samples evenly, and placing the culture flask on a cell culture plate; after all samples were inoculated, the cell culture plates were placed in a 37℃cell incubator. After the blood specimen is cultured in a cell culture bottle for 68-72 hours, a proper amount of colchicine is added for treatment for 30 minutes, and then the sample in the cell culture bottle is poured and transferred into a 15ml centrifuge tube for the subsequent cell harvesting process.
The above operation has the following problems: 1. the apertures of the culture bottle and the 15ml centrifuge tube are smaller, and the situation that the specimen is scattered easily occurs when the poured specimen is transferred; 2. the clinical working sample amount at the present stage is large, and the sample confusion risk exists in the process of transferring the sample; 3. the aluminum cover of the culture bottle is removed, the rubber plug of the culture bottle and the tube cover of the centrifuge tube are opened before transferring the sample, the operation is complex, time and labor are consumed, the aluminum cover is sharp and easy to cause operation damage, the splash of blood samples is easy to occur when the rubber plug is opened, and cross contamination is caused.
At present, a culture tube capable of directly placing a cell culture medium in a 15ml centrifuge tube is available on the market, and can meet the integrated requirements of blood sample culture and harvesting, but the culture tube enables the contact air area of a culture solution to be too small, the cell growth to be limited, the number of harvested cells to be small, the chromosome shape of a metaphase division phase to be poor, and the interpretation and diagnosis of the subsequent chromosome karyotype result to be affected. Thus, there is a need for an integrated cell culture flask that does not affect cell growth and that avoids multiple transfers of specimens.
Disclosure of Invention
Aiming at the defects of the prior art, the utility model provides an integrated cell culture bottle for culturing and harvesting blood samples, which solves the problems that the contact air area of culture solution in a culture tube is too small, the cell growth is limited, the number of cells obtained by harvesting is small, the chromosome shape of a metaphase division phase is poor, and the interpretation and diagnosis of the subsequent chromosome karyotype result are affected.
In order to achieve the above purpose, the utility model is realized by the following technical scheme: the utility model provides an integration cell culture bottle for blood sample is cultivateed and results, includes the toper blake bottle, the toper blake bottle includes body and bottleneck, is provided with first internal thread on the bottleneck inner wall, and the inside fixed toper fill that is used for transferring blood sample that is provided with of bottleneck, the fixed honeycomb duct that is provided with in toper fill top, the liquid mouth that annotates that is used for injecting blood sample and culture medium has been seted up on the body outer wall, the inside cylindrical plug that is used for sealedly that is provided with of liquid mouth, bottleneck internal thread connection has a harvesting pipe subassembly, and harvesting pipe subassembly includes harvesting pipe, scale mark and external screw thread, the scale mark sets up on the harvesting pipe outer wall, the external screw thread sets up in harvesting pipe outer wall below for form threaded connection structure with first internal screw thread, harvesting pipe one end is provided with the harvesting pipe lid subassembly that is used for the harvesting pipe to seal, and harvesting pipe lid subassembly includes harvesting pipe lid and second internal screw thread, the second internal screw thread sets up on the inner wall of harvesting pipe lid.
Further, the culture bottle is in a conical bottle shape, the volume is 20ml, and the culture bottle is made of PET or PETG.
Furthermore, the materials of the conical hopper, the guide pipe and the harvesting pipe are hard plastics.
Furthermore, the harvesting pipe cover is made of hard plastic, and the second internal thread is matched with the external thread.
Furthermore, the harvesting tube is in the shape of a centrifuge tube, has a volume of 15ml and a length of 121.4mm, and is made of hard plastic.
Further, the harvest tube has a capacity of no more than 5ml of cell culture medium pre-stored.
Advantageous effects
The utility model provides an integrated cell culture bottle for culturing and harvesting blood samples. Compared with the prior art, the method has the following beneficial effects:
1. an integrated cell culture bottle for culturing and harvesting blood samples uses the same consumable product through sample inoculation and sample harvesting, the blood samples are located in an integrated culture bottle before harvesting, a conical culture bottle and a harvesting tube are detachably connected through threads, and a transfer tube can drain cultured cells into the harvesting tube, a culture bottle cap is not required to be removed before transferring the samples, a culture bottle plug and a tube cap of a centrifuge tube are opened, the operation process is simplified, and sample scattering or sample confusion caused by sample transfer is avoided.
2. An integrated cell culture bottle for culturing and harvesting blood specimens is characterized in that inoculated blood specimens grow in a culture bottle with a large space, so that cells are in a good growth state, and qualified analysis samples are obtained through subsequent experimental operation.
Drawings
FIG. 1 is a schematic perspective view showing the assembly state of a conical flask and a harvesting tube according to the present utility model;
FIG. 2 is a perspective view showing a separated state of a conical flask and a harvesting tube according to the present utility model;
FIG. 3 is a schematic cross-sectional view of the present utility model;
FIG. 4 is a schematic view showing the structure of the harvesting tube cover and the harvesting tube in a separated state according to the present utility model.
In the figure: 1. a conical flask; 2. a first internal thread; 3. a conical hopper; 4. a flow guiding pipe; 5. a liquid injection port; 6. a cylindrical rubber plug; 7. harvesting the tube; 8. scale marks; 9. an external thread; 10. harvesting a tube cover; 11. and a second internal thread.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The utility model provides a technical scheme that:
one embodiment is shown in fig. 1-4: the utility model provides an integration cell culture bottle for blood sample is cultivateed and results, including toper blake bottle 1, toper blake bottle 1 includes body and bottleneck, be provided with first internal thread 2 on the bottleneck inner wall, and the inside fixed toper fill 3 that is used for transferring blood sample that is provided with of bottleneck, the fixed honeycomb duct 4 that is provided with in toper fill 3 top, be provided with the notes liquid mouth 5 that is used for injecting blood sample and culture medium on the body outer wall, the inside cylindrical plug 6 that is used for sealedly that is provided with of notes liquid mouth 5, bottleneck internal thread connection has the harvesting pipe subassembly, the harvesting pipe subassembly includes harvesting pipe 7, scale mark 8 and external screw thread 9, the scale mark 8 sets up on the harvesting pipe 7 outer wall, external screw thread 9 sets up in harvesting pipe 7 outer wall below, be used for forming threaded connection structure with first internal screw thread 2, harvesting pipe 7 one end is provided with the harvesting pipe cap subassembly that is used for the harvesting pipe 7 sealedly, harvesting pipe cap subassembly includes harvesting pipe cap 10 and second internal screw thread 11, second internal screw thread 11 sets up on the inner wall of harvesting pipe cap 10. The conical flask 1 is in a conical flask shape, the volume is 20ml, the material is PET or PETG, the materials of the conical hopper 3, the flow guide pipe 4 and the harvesting pipe 7 are all hard plastics, the material of the harvesting pipe cover 10 is hard plastics, the second internal thread 11 is matched with the external thread 9, the harvesting pipe 7 is in a centrifuge tube shape, the volume is 15ml, the length is 121.4mm, the material is hard plastics, and the harvesting pipe 7 has a capacity of prestoring not more than 5ml of cell culture medium.
When in use, after the sample is collected, sample information is attached to the harvesting pipe 7; sucking 0.5ml of blood sample by using an injector, injecting the sample into culture by using a cylindrical rubber plug 6 on a conical flask 1, shaking and mixing uniformly, placing the sample into a culture plate, and placing the sample into a culture box for culture for 68-72 hours after the inoculation of all the samples is completed; after the cultivation is finished, the colchicine is sucked by an injector, and a proper amount of colchicine is injected into the conical flask 1 through the cylindrical rubber plug 6 on the conical flask; after the sample is shaken up, the integrated cell culture flask is turned over to allow the sample to enter the harvest tube 7 from the flask 1. The first internal thread 2 and the external thread 9 are loosened by screwing the harvesting tube 7, so that the conical flask 1 is separated from the harvesting tube 7, and the sample in the harvesting tube 7 is subjected to subsequent experimental operations. After the operation is completed, the harvesting tube cover 10 is covered on the harvesting tube 7, and the sample can be stored for later use.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. An integrated cell culture bottle for culturing and harvesting blood specimens, which comprises a conical culture bottle (1), and is characterized in that: the conical culture bottle (1) comprises a bottle body and a bottle neck, a first internal thread (2) is arranged on the inner wall of the bottle neck, a conical bucket (3) for transferring blood samples is fixedly arranged on the inner wall of the bottle neck, a flow guide pipe (4) is fixedly arranged at the top of the conical bucket (3), a liquid injection port (5) for injecting blood samples and culture medium is formed in the outer wall of the bottle body, a cylindrical rubber plug (6) for sealing is arranged in the liquid injection port (5), a harvesting pipe assembly is connected with the inner thread of the bottle neck, the harvesting pipe assembly comprises a harvesting pipe (7), a scale mark (8) and external threads (9), the scale mark (8) is arranged on the outer wall of the harvesting pipe (7), the external threads (9) are arranged below the outer wall of the harvesting pipe (7) and form a threaded connection structure with the first internal threads (2), a harvesting pipe cover assembly for sealing the harvesting pipe (7) is arranged at one end, the harvesting pipe cover assembly comprises a harvesting pipe cover (10) and a second internal thread (11), and the second internal thread (11) is arranged on the inner wall of the harvesting pipe cover (10).
2. An integrated cell culture flask for blood specimen culture and harvest as claimed in claim 1, wherein: the conical flask (1) is in a conical flask shape, the volume is 20ml, and the material is PET or PETG.
3. An integrated cell culture flask for blood specimen culture and harvest as claimed in claim 1, wherein: the cone hopper (3), the guide pipe (4) and the harvesting pipe (7) are made of hard plastics.
4. An integrated cell culture flask for blood specimen culture and harvest as claimed in claim 1, wherein: the harvesting pipe cover (10) is made of hard plastic, and the second internal thread (11) is matched with the external thread (9).
5. An integrated cell culture flask for blood specimen culture and harvest as claimed in claim 1, wherein: the harvesting tube (7) is in the shape of a centrifuge tube, has a volume of 15ml and a length of 121.4mm, and is made of hard plastic.
6. An integrated cell culture flask for blood specimen culture and harvest as claimed in claim 1, wherein: the harvesting tube (7) has a capacity of pre-stored no more than 5ml of cell culture medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321191715.0U CN219839713U (en) | 2023-05-17 | 2023-05-17 | Integrated cell culture bottle for culturing and harvesting blood specimens |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321191715.0U CN219839713U (en) | 2023-05-17 | 2023-05-17 | Integrated cell culture bottle for culturing and harvesting blood specimens |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219839713U true CN219839713U (en) | 2023-10-17 |
Family
ID=88299757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321191715.0U Active CN219839713U (en) | 2023-05-17 | 2023-05-17 | Integrated cell culture bottle for culturing and harvesting blood specimens |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219839713U (en) |
-
2023
- 2023-05-17 CN CN202321191715.0U patent/CN219839713U/en active Active
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