CN206940909U - It is easy to just put the NK cell culture apparatus of micro- sem observation - Google Patents

It is easy to just put the NK cell culture apparatus of micro- sem observation Download PDF

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Publication number
CN206940909U
CN206940909U CN201720731889.XU CN201720731889U CN206940909U CN 206940909 U CN206940909 U CN 206940909U CN 201720731889 U CN201720731889 U CN 201720731889U CN 206940909 U CN206940909 U CN 206940909U
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Prior art keywords
probe tube
ware
tube
cell
peep hole
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CN201720731889.XU
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张巍
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Chongqing Precision Biological Industry Technology Research Institute Co Ltd
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Chongqing Precision Biological Industry Technology Research Institute Co Ltd
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Abstract

The utility model discloses a kind of NK cell culture apparatus for being easy to just put micro- sem observation, including ware body and ware lid;The ware, which covers, is provided with peep hole, and pore-throat is provided with the outside of the peep hole, port lid is provided with the pore-throat;Probe tube is provided with the peep hole, the upper end mouth rim of the probe tube is conveniently placed at ware and covered, and the lower end oral area enclosed shape of probe tube is provided with culture medium and passes through hole into slide, the lower end side of probe tube;It is provided with isolated tube in the probe tube, the lower end oral area enclosed shape of the isolated tube is into cover glass.This NK cell culture apparatus section, which is placed directly in, just puts microscope cell is observed, and need not take out cell during observation, observation is simple and convenient;And object lens are isolated pipe isolation when observing so that object lens with culture medium and cells contacting, can not avoid polluting.

Description

It is easy to just put the NK cell culture apparatus of micro- sem observation
Technical field
Technical field of cell culture is the utility model is related to, more particularly to a kind of NK Tissue Culture Dish.
Background technology
NK (natural killer cell, NK cell) is the important immunocyte of body, not only with resisting Tumour, viral infection resisting and immunological regulation are relevant, and participate in hypersensitivity and autoimmune disease in some cases Occur.
With the development of biological therapy clinical practice, NK cells are more and more extensive in the research and application of medical field.Will It is used for clinical treatment using NK cells, it is necessary first to expand substantial amounts of NK cells in vitro.The process of NK cells is cultivated in vitro In, often the growth conditions of cell are observed.But existing Tissue Culture Dish, which lacks, can realize that just putting microscope sees The structure examined so that cell observation will use inverted microscope, or need to take out cell from culture dish.And it is inverted micro- Mirror utilization rate is relatively low, and price is higher than just putting microscope, and inverted microscope, therefore cell observation are not prepared in general laboratory Cell is taken out and is just putting the mode of micro- Microscopic observation by generally use from culture dish.But cell is taken out from culture dish The problem of complex operation be present again in observation.
Utility model content
In view of this, the purpose of this utility model is to provide a kind of NK cell culture dress for being easy to just put micro- sem observation Put, can not be placed directly in to solve existing culture dish and just put the technical problem observed under microscope.
The utility model is easy to just put the NK cell culture apparatus of micro- sem observation, including ware body and ware lid;The ware lid On be provided with peep hole, pore-throat is provided with the outside of the peep hole, port lid is provided with the pore-throat;
Probe tube is provided with the peep hole, the upper end mouth rim of the probe tube is conveniently placed at ware and covered, takes The lower end oral area enclosed shape of sample pipe is provided with culture medium and passes through hole into slide, the lower end side of probe tube;
It is provided with isolated tube in the probe tube, the lower end oral area enclosed shape of the isolated tube is into cover glass.
The beneficial effects of the utility model:
The utility model is easy to just put the NK cell culture apparatus of micro- sem observation, and it in ware by covering setting observation Hole, and probe tube and isolated tube are set in the air in observation, when needing cell growth state to be observed, port lid is opened, will Probe tube inserts peep hole, and the culture medium and cell in ware body flow into probe tube from culture medium by hole, are inserted into isolated tube, Unnecessary cell and culture medium are discharged probe tube by isolated tube;Then NK cell culture apparatus is placed on and just put under microscope, and Object lens are put into isolated tube, you can cell is observed, cell need not be taken out during observation, observation is simple and convenient;And Object lens are isolated pipe isolation during observation so that object lens with culture medium and cells contacting, can not avoid polluting.
Brief description of the drawings
Fig. 1 is the sectional structure signal for the NK cell culture apparatus that the utility model is easy to just put micro- sem observation.
Embodiment
The utility model is further described with reference to the accompanying drawings and examples.
As illustrated, the present embodiment is easy to just put the NK cell culture apparatus of micro- sem observation, including ware body 1 and ware lid 2; The ware, which covers, is provided with peep hole, and pore-throat 3 is provided with the outside of the peep hole, port lid 4 is provided with the pore-throat;
Probe tube 5 is provided with the peep hole, the upper end mouth rim of the probe tube is conveniently placed at ware and covered, takes The lower end oral area enclosed shape of sample pipe is provided with culture medium and passes through hole 7 into slide 6, the lower end side of probe tube;
It is provided with isolated tube 8 in the probe tube, the lower end oral area enclosed shape of the isolated tube is into cover glass 9.
The present embodiment is easy to just put the NK cell culture apparatus of micro- sem observation, its by covering setting peep hole in ware, And probe tube and isolated tube are set in the air in observation, when needing cell growth state to be observed, port lid is opened, will be sampled Pipe inserts peep hole, and the culture medium and cell in ware body just flow into probe tube from culture medium by hole, are inserted into isolated tube, every Unnecessary cell and culture medium are discharged into probe tube from pipe;Then NK cell culture apparatus is placed on and just put under microscope, and will Object lens are put into isolated tube, you can cell is observed, need not be taken out cell during observation, observation is simple and convenient;And see Object lens are isolated pipe isolation when examining so that object lens with culture medium and cells contacting, can not avoid polluting.
Finally illustrate, above example is only unrestricted to illustrate the technical solution of the utility model, although ginseng The utility model is described in detail according to preferred embodiment, it will be understood by those within the art that, can be to this The technical scheme of utility model is modified or equivalent substitution, without departing from the objective and model of technical solutions of the utility model Enclose, it all should cover among right of the present utility model.

Claims (1)

1. a kind of NK cell culture apparatus for being easy to just put micro- sem observation, including ware body and ware lid;It is characterized in that:The ware Cover and be provided with peep hole, pore-throat is provided with the outside of the peep hole, port lid is provided with the pore-throat;
Probe tube is provided with the peep hole, the upper end mouth rim of the probe tube is conveniently placed at ware and covered, probe tube Lower end oral area enclosed shape be provided with culture medium into slide, the lower end side of probe tube and pass through hole;
It is provided with isolated tube in the probe tube, the lower end oral area enclosed shape of the isolated tube is into cover glass.
CN201720731889.XU 2017-06-19 2017-06-19 It is easy to just put the NK cell culture apparatus of micro- sem observation Active CN206940909U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720731889.XU CN206940909U (en) 2017-06-19 2017-06-19 It is easy to just put the NK cell culture apparatus of micro- sem observation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720731889.XU CN206940909U (en) 2017-06-19 2017-06-19 It is easy to just put the NK cell culture apparatus of micro- sem observation

Publications (1)

Publication Number Publication Date
CN206940909U true CN206940909U (en) 2018-01-30

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720731889.XU Active CN206940909U (en) 2017-06-19 2017-06-19 It is easy to just put the NK cell culture apparatus of micro- sem observation

Country Status (1)

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CN (1) CN206940909U (en)

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