CN204874523U - Biological sample preparation pipe - Google Patents
Biological sample preparation pipe Download PDFInfo
- Publication number
- CN204874523U CN204874523U CN201520603951.8U CN201520603951U CN204874523U CN 204874523 U CN204874523 U CN 204874523U CN 201520603951 U CN201520603951 U CN 201520603951U CN 204874523 U CN204874523 U CN 204874523U
- Authority
- CN
- China
- Prior art keywords
- pipe
- pipe lid
- process pipe
- bacterium
- lid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a biological sample preparation pipe, including body and tube cap, body one end is sealed, and the other end is the open end, the tube cap is used for the open end of sealed body, and the connection can be dismantled to tube cap and body, sample preparation liquid and many grinding pearls are equipped with in the body. The utility model discloses a handle the pipe and can handle multiple sample, come wherein bacterium, virus, cell dispersion, do not harm the bacterium isotructure again for the bacterium is cultivateed more easily, and it is convenient, swift to handle, and the yield is high, and is effectual.
Description
Technical field
The utility model belongs to medical science, relates to a kind of biological specimen process pipe.
Background technology
Medical research and clinical in, often need the separation and Culture of the biological specimen of patient being carried out to bacterium or virus.The separation and Culture of bacterium in current biological specimen, virus and cell mainly comprises following several:
The separation and Culture (as sputum, nasal mucus) of bacterium in sample: directly by sample liquid normal saline dilution, be coated on plate culture medium and cultivate.But the composition in sputum or nasal mucus liquid is numerous and diverse, directly cultivated by Sample Dilution, bacterial growth effect is bad, and is coated on also not easily dispersed growth on substratum.
The separation and Culture of cell in tissue samples, bacterium or virus: the method for the cell in existing separation and Extraction tissue, bacterium or virus mainly contains enzymolysis process, liquid nitrogen grinding method etc., and these methods by the cellular segregation in tissue out, can carry out cell cultures.But, long by the enzymolysis process isolated cell time; And liquid nitrogen grinding method operational requirement is high, larger to the loss of cell, be unfavorable for that the later stage of cell cultivates.
Utility model content
The purpose of this utility model is to provide a kind of new biological specimen process pipe; to realize processing biological specimen; bacterium wherein, virus are spread out; do not damage the structures such as bacterium again; bacterium is made more easily to cultivate or the cellular segregation in animal and plant tissue be come; or the bacterium discharged in tissue and virus, protect its cell be separated, bacterium, viruses etc. simultaneously, so that the object of cultivating.
For achieving the above object, the utility model is taked following technical proposals to realize:
A kind of biological specimen process pipe, comprises body and Guan Gai;
Body one end seals, and the other end is opening end;
Pipe lid is used for the opening end of sealed tube body, and pipe lid and body removably connect;
Body is built with sample treatment solution and many grinding bead.
Further, the diameter of grinding bead is 0.1mm-5mm.
Further, grinding bead is zirconium oxide bead.
Further, pipe lid and body are threaded connection.
Further, also sealing-ring is provided with between pipe lid and body.
Further, the outer side of pipe lid is provided with anti-slip veins, and body top is also provided with anti-slip veins.
Further, pipe lid inside is provided with tubular insertion section, and insertion section inwall is provided with multiple projection.
Compared with prior art, the utility model has the following advantages:
Process pipe of the present utility model adopts the pipe lid that the body of inertia high strength and stopping property are strong, and the grinding bead of high strength realizes the grinding to biological specimen, thus the bacterium in biological specimen and virus etc. is discharged and also evenly scatter.Only need in sample treatment solution in pending sample immersion process pipe during use, the process pipe filling pending sample is put into grinding instrument to grind, after completing, open pipe lid, get the sample processed and can carry out the purposes such as next step cell, bacteriophage cultivation.
The release such as bacterium, virus in sample (sputum, nasal mucus liquid) can effectively also spread out by process pipe of the present utility model uniformly, uses in order to cultivating; Also can by the cellular segregation in various tissue out; Bacterium, the virus of invasion tissue discharge; And the basic structure of not damaging cells or bacteriophage, process convenient, fast, yield is high, effective.
Accompanying drawing explanation
Fig. 1 is the structural representation of biological specimen process pipe in the utility model embodiment 1.
Fig. 2 is the assembling schematic diagram of biological specimen process pipe body and pipe lid in the utility model embodiment 2.
Fig. 3 is the cross-sectional schematic along A-A direction in Fig. 2.
Fig. 4 is the structural representation of Fig. 2 middle tube body.
Fig. 5 is the structural representation of pipe lid in Fig. 2.
Fig. 6 is the cross-sectional schematic along B-B direction in Fig. 5.
Embodiment
Below in conjunction with specific embodiment, the utility model is elaborated.
Embodiment 1
Fig. 1 is the structural representation of biological specimen process pipe in the present embodiment.As shown in Figure 1, the biological specimen process pipe of the present embodiment, comprises body 1 and pipe lid 2.
Body 1 is built with sample treatment solution 4 and many grinding bead 5.
Body 1 of the present utility model, in order to satisfied grinding needs, preferably adopts inertia high strength Polymer materialspreparation.
The volume of body 1 of the present invention, needs to be arranged to plurality of specifications according to difference.
Body 1 one end seals, and the other end is opening end.
Pipe lid 2 is for the opening end of sealed tube body 1, and pipe lid 2 stopping property is good.Pipe lid 2 and body 1 removably connect.
Sample treatment solution is different according to the specification of body 1, and volume is also different, and in general, preferably, the volume of sample treatment solution is 2/3rds of body volume, and this kind of volume is conducive to abundant grinding and the process of biological specimen most.
Sample treatment solution composition is bovine serum albumin, EDTA2Na and damping fluid.
Damping fluid of the present utility model can be physiological saline, phosphoric acid buffer, 4-hydroxyethyl piperazine ethanesulfonic acid damping fluid, the one in Tris damping fluid.The pH value of damping fluid is 7.0-7.4.In the present embodiment, damping fluid is physiological saline, and pH value is 7.2.
The pearl that grinding bead selects hardness large, selects zirconium oxide bead in the present embodiment.
The diameter of grinding bead is generally 0.1mm-5mm, can select grinding bead according to pending sample particular case.The diameter of the present embodiment middle tube body 1 is 1cm, and the diameter of grinding bead selects 3mm.
The quantity of grinding bead degree of grind that is different according to the specification of body 1 and sample needs is different, and several to tens are not etc.
Only need in sample treatment solution in pending sample immersion process pipe during use, the process pipe filling pending sample is put into the transfer of grinding instrument to move, grinding bead is ground sample, after completing, open pipe lid, get the sample processed and can carry out the purposes such as next step cell, bacteriophage cultivation.
Embodiment 2:
Fig. 2 is the assembling schematic diagram of biological specimen process pipe body and pipe lid in the present embodiment.Fig. 3 is the sectional view along A-A direction in Fig. 2.Composition graphs 1 and Fig. 2 known, the biological specimen process pipe of the present embodiment, comprises body 1 and pipe lid 2.
Body 1 upper opening, is connected by thread seal with pipe lid 2.
In order to realize body 1 and pipe lid 2 is better tightly connected, between body 1 top and pipe lid 2, be also provided with sealing-ring 3.
Be provided with conical lower portion 15 bottom body 1, the height of the centre of this conical lower portion 15 is greater than surrounding.Consider the pressure of grinding to body 1, this kind of design bottom body 1 can make body 1 more durable.
For the ease of processing the placement of pipe, be provided with cylinder around body 1 conical lower portion 15, the external diameter of this cylindrical structure is identical with the external diameter of body.
Fig. 4 is the structural representation of Fig. 2 middle tube body 1.As shown in Figure 4, the top of body 1 is provided with outside screw 11.This outside screw 11 for matching with the internal thread of pipe lid 2, to realize being tightly connected of body 1 and pipe lid 2.
The bottom at outside screw 11 position is provided with surrounding edge 12.Surrounding edge 12 is for limiting the link position of pipe lid 2.
Surrounding edge 12 bottom keeps at a certain distance away and is provided with a circle card article 13, and the diameter of card article 13 diminishes in direction at the bottom of along the mouth of pipe to pipe gradually.Card article 13 is for being held on the grinding frame in grinding instrument by process pipe.
Also be provided with anti-slip veins 14 below surrounding edge 12, anti-skidding effect can be played in use.
Fig. 5 is the structural representation of pipe lid 2 in Fig. 2.Fig. 6 is the sectional view along B-B direction in Fig. 5.Composition graphs 5 and Fig. 6 known, pipe lid 2 inwall is provided with internal thread 21, for matching with the outside screw 11 of body 1, to realize being tightly connected of body 1 and pipe lid 2.
Pipe lid 2 inside is also provided with insertion section 22, and this insertion section 22 is tubular, is fixed on pipe lid 2 top inner surface at the bottom of cylinder, and body 1, when assembling, is inserted inner in one end relative at the bottom of cylinder, to realize being tightly connected of pipe lid 2 and body 1 further.
The inwall of insertion section 22 is arranged three projections 23, this projection 23, for reinforcing insertion section 22, increases frictional force when grinding simultaneously.
Pipe lid 2 outer side is also provided with a circle anti-slip veins, and person easy to use tightens or unclamps bottle cap.
Although the utility model discloses as above with preferred embodiment, the utility model is not defined in this.Any those skilled in the art, not departing from spirit and scope of the present utility model, all can make various changes or modifications, and therefore protection domain of the present utility model should be as the criterion with claim limited range.
Claims (8)
1. biological specimen process pipe, is characterized in that, described process pipe comprises body and Guan Gai,
Body one end seals, and the other end is opening end;
Pipe lid is used for the opening end of sealed tube body, and pipe lid and body removably connect;
Body is built with sample treatment solution and many grinding bead.
2. process pipe according to claim 1, it is characterized in that, the diameter of grinding bead is 0.1mm-5mm.
3. process pipe according to claim 1, it is characterized in that, pipe lid and body are threaded connection.
4. process pipe according to claim 1, is characterized in that, be also provided with sealing-ring between pipe lid and body.
5. process pipe according to claim 1, it is characterized in that, the outer side of pipe lid is provided with anti-slip veins.
6. process pipe according to claim 1, it is characterized in that, body top is provided with anti-slip veins.
7. process pipe according to claim 1, it is characterized in that, pipe lid inside is provided with tubular insertion section.
8. process pipe according to claim 7, it is characterized in that, insertion section inwall is provided with multiple projection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520603951.8U CN204874523U (en) | 2015-08-12 | 2015-08-12 | Biological sample preparation pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520603951.8U CN204874523U (en) | 2015-08-12 | 2015-08-12 | Biological sample preparation pipe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204874523U true CN204874523U (en) | 2015-12-16 |
Family
ID=54818920
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520603951.8U Active CN204874523U (en) | 2015-08-12 | 2015-08-12 | Biological sample preparation pipe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204874523U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105154321A (en) * | 2015-08-12 | 2015-12-16 | 杭州创新生物检控技术有限公司 | Biological sample treatment tube |
| CN114408843A (en) * | 2022-02-24 | 2022-04-29 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Filling all-in-one machine and control method thereof |
-
2015
- 2015-08-12 CN CN201520603951.8U patent/CN204874523U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105154321A (en) * | 2015-08-12 | 2015-12-16 | 杭州创新生物检控技术有限公司 | Biological sample treatment tube |
| CN114408843A (en) * | 2022-02-24 | 2022-04-29 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Filling all-in-one machine and control method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101984049B (en) | Method for separating mesenchymal stem cells from dispose tissues | |
| CN204608026U (en) | A kind of cell with super hydrophilic growth surface embeds ware | |
| CN101298606A (en) | Preparation, storage and use of umbilical cord and placenta mesenchymal stem cell for clinical therapy | |
| US9549715B2 (en) | Vial useable in tissue extraction procedures | |
| CN204874523U (en) | Biological sample preparation pipe | |
| CN109486753A (en) | A kind of fat stem cell extracting method | |
| CN106434547A (en) | Method for preparing umbilical cord mesenchymal stem cells | |
| CN106479869A (en) | A kind of cell filter | |
| CN102741424A (en) | Method for separating stem cells | |
| CN105154321A (en) | Biological sample treatment tube | |
| CN205042500U (en) | Cell centrifuging tube | |
| CN204661736U (en) | The special cell culture bags of the automatic incubator of cell | |
| CN103289897A (en) | Cell culture bottle | |
| CN205556658U (en) | Bacterium filter cartridge | |
| CN205011769U (en) | People's peripheral blood chromosome cell culture device | |
| CN205087344U (en) | Biological tissue freezes and deposits container | |
| CN204779581U (en) | Aseptic detection adapter of cell culture | |
| CN207793282U (en) | Culture bottle for the automatic amplification cultivation of cell | |
| CN207210453U (en) | A kind of Tissue Culture Flask | |
| CN104830684A (en) | Special cell culture bag for automatic cell incubator | |
| CN206328381U (en) | A kind of cell filter | |
| CN104840361A (en) | Cord blood collection and separation blood bag | |
| CN109762784A (en) | Application of the Clozapine in the mescenchymal stem cell aging for delaying culture | |
| CN208532780U (en) | The medical stem cell that can be used for open environment sterile changes liquid bottle | |
| CN103952296A (en) | Multifunctional medical treatment substance body combiner |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Yonggang Inventor after: Wu Chuanli Inventor after: Zhu Fanghong Inventor after: Wang Wenjun Inventor after: Hu Xiaofang Inventor before: Li Yonggang Inventor before: Wu Chuanli Inventor before: Zhu Fanghong Inventor before: Wang Wenjun Inventor before: Hu Xiaofang |
|
| COR | Change of bibliographic data |