CN105154321A - Biological sample treatment tube - Google Patents
Biological sample treatment tube Download PDFInfo
- Publication number
- CN105154321A CN105154321A CN201510493625.0A CN201510493625A CN105154321A CN 105154321 A CN105154321 A CN 105154321A CN 201510493625 A CN201510493625 A CN 201510493625A CN 105154321 A CN105154321 A CN 105154321A
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- China
- Prior art keywords
- process pipe
- pipe according
- damping fluid
- pipe lid
- lid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a biological sample treatment tube which comprises a tube body and a tube cover, wherein one end of the tube body is sealed and the other end of the tube body is an open end; the tube cover is used for sealing the open end of the tube body; the tube cover is detachably connected with the tube body; and the tube body is filled with a sample treatment liquid and multiple grinding beads. The treatment tube provided by the invention can treat various biological samples, disperses bacteria, viruses and cells, and does not damage the structures of the bacteria and the like, so that the bacteria are easier to culture, the treatment is convenient and rapid, the yield is high and the effect is good.
Description
Technical field
The invention belongs to medical science, relate to a kind of biological specimen process pipe.
Background technology
Medical research and clinical in, often need the separation and Culture of the biological specimen of patient being carried out to bacterium or virus.The separation and Culture of bacterium in current biological specimen, virus and cell mainly comprises following several:
The separation and Culture (as sputum, nasal mucus) of bacterium in sample: directly by sample liquid normal saline dilution, be coated on plate culture medium and cultivate.But the composition in sputum or nasal mucus liquid is numerous and diverse, directly cultivated by Sample Dilution, bacterial growth effect is bad, and is coated on also not easily dispersed growth on substratum.
The separation and Culture of cell in tissue samples, bacterium or virus: the method for the cell in existing separation and Extraction tissue, bacterium or virus mainly contains enzymolysis process, liquid nitrogen grinding method etc., and these methods by the cellular segregation in tissue out, can carry out cell cultures.But, long by the enzymolysis process isolated cell time; And liquid nitrogen grinding method operational requirement is high, larger to the loss of cell, be unfavorable for that the later stage of cell cultivates.
Summary of the invention
The object of this invention is to provide a kind of new biological specimen process pipe; to realize processing biological specimen; bacterium wherein, virus are spread out; do not damage the structures such as bacterium again; bacterium is made more easily to cultivate or the cellular segregation in animal and plant tissue be come; or the bacterium discharged in tissue and virus, protect its cell be separated, bacterium, viruses etc. simultaneously, so that the object of cultivating.
For achieving the above object, the present invention takes following technical proposals to realize:
A kind of biological specimen process pipe, comprises body and Guan Gai,
Body one end seals, and the other end is opening end;
Pipe lid is used for the opening end of sealed tube body, and pipe lid and body removably connect;
Body is built with sample treatment solution and many grinding bead.
Further, the diameter of grinding bead is 0.1mm-5mm.
Further, grinding bead is zirconium oxide bead.
Further, pipe lid and body are threaded connection.
Further, also sealing-ring is provided with between pipe lid and body.
Further, the outer side of pipe lid is provided with anti-slip veins, and body top is also provided with anti-slip veins.
Further, pipe lid inside is provided with tubular insertion section, and insertion section inwall is provided with multiple projection.
Further, sample treatment solution composition is bovine serum albumin, EDTA2Na and damping fluid.
Further, damping fluid is physiological saline, phosphoric acid buffer, 4-hydroxyethyl piperazine ethanesulfonic acid damping fluid, the one in Tris damping fluid.
Further, the pH value of damping fluid is 7.0-7.4.
Compared with prior art, the present invention has the following advantages:
Process pipe of the present invention adopts the pipe lid that the body of inertia high strength and stopping property are strong, and the grinding bead of high strength realizes the grinding to biological specimen, thus the bacterium in biological specimen and virus etc. is discharged and also evenly scatter.Only need in sample treatment solution in pending sample immersion process pipe during use, the process pipe filling pending sample is put into grinding instrument to grind, after completing, open pipe lid, get the sample processed and can carry out the purposes such as next step cell, bacteriophage cultivation.
The release such as bacterium, virus in sample liquid (sputum, nasal mucus liquid) can effectively also spread out by process pipe of the present invention uniformly, uses in order to cultivating; Also can by the cellular segregation in various tissue out; Bacterium, the virus of invasion tissue discharge; And the basic structure of not damaging cells or bacteriophage, process convenient, fast, yield is high, effective.
Accompanying drawing explanation
Fig. 1 is the structural representation of biological specimen process pipe in the embodiment of the present invention 1.
Fig. 2 is the assembling schematic diagram of biological specimen process pipe body and pipe lid in the embodiment of the present invention 2.
Fig. 3 is the cross-sectional schematic along A-A direction in Fig. 2.
Fig. 4 is the structural representation of Fig. 2 middle tube body.
Fig. 5 is the structural representation of pipe lid in Fig. 2.
Fig. 6 is the cross-sectional schematic along B-B direction in Fig. 5.
Embodiment
Below in conjunction with specific embodiment, the present invention will be described in detail.
Embodiment 1
Fig. 1 is the structural representation of biological specimen process pipe in the present embodiment.As shown in Figure 1, the biological specimen process pipe of the present embodiment, comprises body 1 and pipe lid 2.
Body 1 is built with sample treatment solution 4 and many grinding bead 5.
Body 1 of the present invention, in order to satisfied grinding needs, preferably adopts inertia high strength Polymer materialspreparation.
The volume of body 1 of the present invention, needs to be arranged to plurality of specifications according to difference.
Body 1 one end seals, and the other end is opening end.
Pipe lid 2 is for the opening end of sealed tube body 1, and pipe lid 2 stopping property is good.Pipe lid 2 and body 1 removably connect.
Sample treatment solution is different according to the specification of body 1, and volume is also different, and in general, preferably, the volume of sample treatment solution is 2/3rds of body volume, and this kind of volume is conducive to abundant grinding and the process of biological specimen most.
Common, sample treatment solution volume has 2ml, 5ml, 10ml, 15ml, 50ml plurality of specifications.
Sample treatment solution composition is bovine serum albumin, and its concentration is 10mg/ml, EDTA2Na, and its concentration is 1.5mg/ml and damping fluid.
Damping fluid of the present invention can be physiological saline, phosphoric acid buffer, 4-hydroxyethyl piperazine ethanesulfonic acid damping fluid, the one in Tris damping fluid.The pH value of damping fluid is 7.0-7.4.In the present embodiment, damping fluid is physiological saline, and pH value is 7.2.
The pearl that grinding bead selects hardness large, selects zirconium oxide bead in the present embodiment.
The diameter of grinding bead is generally 0.1mm-5mm, can select grinding bead according to pending sample particular case.The diameter of the present embodiment middle tube body 1 is 1cm, and the diameter of grinding bead selects 3mm.
The quantity of grinding bead degree of grind that is different according to the specification of body 1 and sample needs is different, and several to tens are not etc.
Only need in sample treatment solution in pending sample immersion process pipe during use, the process pipe filling pending sample is put into the transfer of grinding instrument to move, grinding bead is ground sample, after completing, open pipe lid, get the sample processed and can carry out the purposes such as next step cell, bacteriophage cultivation.
Embodiment 2:
Fig. 2 is the assembling schematic diagram of biological specimen process pipe body and pipe lid in the present embodiment.Fig. 3 is the sectional view along A-A direction in Fig. 2.Composition graphs 1 and Fig. 2 known, the biological specimen process pipe of the present embodiment, comprises body 1 and pipe lid 2.
Body 1 upper opening, is connected by thread seal with pipe lid 2.
In order to realize body 1 and pipe lid 2 is better tightly connected, between body 1 top and pipe lid 2, be also provided with sealing-ring 3.
Be provided with conical lower portion 15 bottom body 1, the height of the centre of this conical lower portion 15 is greater than surrounding.Consider the pressure of grinding to body 1, this kind of design bottom body 1 can make body 1 more durable.
For the ease of processing the placement of pipe, be provided with cylinder around body 1 conical lower portion 15, the external diameter of this cylindrical structure is identical with the external diameter of body.
Fig. 4 is the structural representation of Fig. 2 middle tube body 1.As shown in Figure 4, the top of body 1 is provided with outside screw 11.This outside screw 11 for matching with the internal thread of pipe lid 2, to realize being tightly connected of body 1 and pipe lid 2.
The bottom at outside screw 11 position is provided with surrounding edge 12.Surrounding edge 12 is for limiting the link position of pipe lid 2.
Surrounding edge 12 bottom keeps at a certain distance away and is provided with a circle card article 13, and the diameter of card article 13 diminishes in direction at the bottom of along the mouth of pipe to pipe gradually.Card article 13 is for being held on the grinding frame in grinding instrument by process pipe.
Also be provided with anti-slip veins 14 below surrounding edge 12, anti-skidding effect can be played in use.
Fig. 5 is the structural representation of pipe lid 2 in Fig. 2.Fig. 6 is the sectional view along B-B direction in Fig. 5.Composition graphs 5 and Fig. 6 known, pipe lid 2 inwall is provided with internal thread 21, for matching with the outside screw 11 of body 1, to realize being tightly connected of body 1 and pipe lid 2.
Pipe lid 2 inside is also provided with insertion section 22, and this insertion section 22 is tubular, is fixed on pipe lid 2 top inner surface at the bottom of cylinder, and body 1, when assembling, is inserted inner in one end relative at the bottom of cylinder, to realize being tightly connected of pipe lid 2 and body 1 further.
The inwall of insertion section 22 is arranged three projections 23, this projection 23, for reinforcing insertion section 22, increases frictional force when grinding simultaneously.
Pipe lid 2 outer side is also provided with a circle anti-slip veins, and person easy to use tightens or unclamps bottle cap.
Although the present invention with preferred embodiment openly as above; but it is not for limiting the present invention; any those skilled in the art without departing from the spirit and scope of the present invention; the process pipe of above-mentioned announcement and technology contents can be utilized to make possible variation and amendment to technical solution of the present invention; therefore; every content not departing from technical solution of the present invention; according to technical spirit of the present invention to any simple modification made for any of the above embodiments, equivalent variations and modification, all belong to the protection domain of technical solution of the present invention.
Claims (10)
1. biological specimen process pipe, is characterized in that, described process pipe comprises body and Guan Gai,
Body one end seals, and the other end is opening end;
Pipe lid is used for the opening end of sealed tube body, and pipe lid and body removably connect;
Body is built with sample treatment solution and many grinding bead.
2. process pipe according to claim 1, it is characterized in that, the diameter of grinding bead is 0.1mm-5mm.
3. process pipe according to claim 1, it is characterized in that, grinding bead is zirconium oxide bead.
4. process pipe according to claim 1, it is characterized in that, pipe lid and body are threaded connection.
5. process pipe according to claim 1, is characterized in that, be also provided with sealing-ring between pipe lid and body.
6. process pipe according to claim 1, it is characterized in that, the outer side of pipe lid is provided with anti-slip veins, and body top is also provided with anti-slip veins.
7. process pipe according to claim 1, it is characterized in that, pipe lid inside is provided with tubular insertion section, and insertion section inwall is provided with multiple projection.
8. process pipe according to claim 1, it is characterized in that, sample treatment solution composition is bovine serum albumin, EDTA2Na and damping fluid.
9. process pipe according to claim 8, it is characterized in that, damping fluid is physiological saline, phosphoric acid buffer, 4-hydroxyethyl piperazine ethanesulfonic acid damping fluid, the one in Tris damping fluid.
10. process pipe according to claim 8 is characterized in that the pH value of damping fluid is 7.0-7.4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510493625.0A CN105154321A (en) | 2015-08-12 | 2015-08-12 | Biological sample treatment tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510493625.0A CN105154321A (en) | 2015-08-12 | 2015-08-12 | Biological sample treatment tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105154321A true CN105154321A (en) | 2015-12-16 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510493625.0A Pending CN105154321A (en) | 2015-08-12 | 2015-08-12 | Biological sample treatment tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105154321A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106238117A (en) * | 2016-08-31 | 2016-12-21 | 黄捷 | A kind of test tube and method being centrifuged pretreatment for saliva DNA |
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| CN102032998A (en) * | 2009-09-29 | 2011-04-27 | 帕斯维基因组学公司 | Saliva sample collecting system |
| CN201962287U (en) * | 2010-12-27 | 2011-09-07 | 青岛高科技工业园海博生物技术有限公司 | Strain preservative tube |
| CN202057530U (en) * | 2011-03-21 | 2011-11-30 | 泸州医学院 | Micro grinder |
| CN102271814A (en) * | 2008-10-31 | 2011-12-07 | 生物梅里埃公司 | Separation device for use in the separation, characterization and/or identification of microorganisms |
| CN202485960U (en) * | 2012-03-19 | 2012-10-10 | 三明市和众生物技术有限公司 | Disposable tissue grinding tube |
| CN202881269U (en) * | 2012-11-22 | 2013-04-17 | 济南百博生物技术有限责任公司 | Mycobacterium tuberculosis grinding tube |
| CN103890163A (en) * | 2011-06-19 | 2014-06-25 | 阿博根公司 | Devices, solutions and methods for sample collection |
| CN203981468U (en) * | 2014-07-09 | 2014-12-03 | 朱童心 | A kind of cell beveller |
| CN204385198U (en) * | 2015-01-13 | 2015-06-10 | 友康恒业生物科技（北京）有限公司 | A kind of strain preservative tube |
| CN204874523U (en) * | 2015-08-12 | 2015-12-16 | 杭州创新生物检控技术有限公司 | Biological sample preparation pipe |
-
2015
- 2015-08-12 CN CN201510493625.0A patent/CN105154321A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101370425A (en) * | 2005-12-09 | 2009-02-18 | Dna吉诺特克股份有限公司 | Container system for releasably storing a substance |
| CN102271814A (en) * | 2008-10-31 | 2011-12-07 | 生物梅里埃公司 | Separation device for use in the separation, characterization and/or identification of microorganisms |
| CN102032998A (en) * | 2009-09-29 | 2011-04-27 | 帕斯维基因组学公司 | Saliva sample collecting system |
| CN201962287U (en) * | 2010-12-27 | 2011-09-07 | 青岛高科技工业园海博生物技术有限公司 | Strain preservative tube |
| CN202057530U (en) * | 2011-03-21 | 2011-11-30 | 泸州医学院 | Micro grinder |
| CN103890163A (en) * | 2011-06-19 | 2014-06-25 | 阿博根公司 | Devices, solutions and methods for sample collection |
| CN202485960U (en) * | 2012-03-19 | 2012-10-10 | 三明市和众生物技术有限公司 | Disposable tissue grinding tube |
| CN202881269U (en) * | 2012-11-22 | 2013-04-17 | 济南百博生物技术有限责任公司 | Mycobacterium tuberculosis grinding tube |
| CN203981468U (en) * | 2014-07-09 | 2014-12-03 | 朱童心 | A kind of cell beveller |
| CN204385198U (en) * | 2015-01-13 | 2015-06-10 | 友康恒业生物科技（北京）有限公司 | A kind of strain preservative tube |
| CN204874523U (en) * | 2015-08-12 | 2015-12-16 | 杭州创新生物检控技术有限公司 | Biological sample preparation pipe |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106238117A (en) * | 2016-08-31 | 2016-12-21 | 黄捷 | A kind of test tube and method being centrifuged pretreatment for saliva DNA |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Yonggang Inventor after: Wu Chuanli Inventor after: Zhu Fanghong Inventor after: Wang Wenjun Inventor after: Hu Xiaofang Inventor before: Li Yonggang Inventor before: Wu Chuanli Inventor before: Zhu Fanghong Inventor before: Wang Wenjun Inventor before: Hu Xiaofang |
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| COR | Change of bibliographic data | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151216 |
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| RJ01 | Rejection of invention patent application after publication |