CN208857309U - A kind of cell culture centrifuge tube - Google Patents
A kind of cell culture centrifuge tube Download PDFInfo
- Publication number
- CN208857309U CN208857309U CN201821363280.2U CN201821363280U CN208857309U CN 208857309 U CN208857309 U CN 208857309U CN 201821363280 U CN201821363280 U CN 201821363280U CN 208857309 U CN208857309 U CN 208857309U
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- CN
- China
- Prior art keywords
- cell culture
- centrifuge tube
- tube body
- sealing cover
- planchet
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Centrifugal Separators (AREA)
Abstract
The utility model discloses a kind of cell culture centrifuge tubes, including tube body, the sealing cover being connect with tube body, the sealing lid top wall is equipped with aperture, the aperture is embedded with soft plug, and the soft plug includes the lower planchet for extending to the roof lower surface, and the lower planchet is compressed when sealing cover covers tightly tube body by the open end of tube body, by using the soft plug structure that can resist body open, sealing performance is further improved compared with prior art.
Description
Technical field
The utility model belongs to field of biotechnology more particularly to a kind of cell culture centrifuge tube.
Background technique
During basic scientific research and clinical examination, in order to be proliferated specific cells, it is necessary to carry out cell culture, amplification is thin
Born of the same parents' quantity.General cell culture is cultivated in culture bottle or culture dish, when harvesting cell progress subsequent detection, is needed
It is transferred to and carries out that cell is collected by centrifugation in centrifuge tube.Such transfer operation is easily introduced pollution, cause subsequent experimental or
Detection is interfered.Current solution is directly to inject culture medium in general centrifuge tube, cell is trained in centrifuge tube
It supports, then when harvesting cell, is directly centrifuged, does not need a cell by culture bottle and be transferred to centrifuge tube.But this method exists
When cell is added, sealing cap assemblies is still unscrewed, it is inconvenient.As shown in figure 5, Chinese patent CN206014393U is public
A kind of improvement project has been opened, by designing rubber plug at sealing cover center, cell has been injected directly with syringe needle, is not required to out
Lid.But it is discovered by experiment that sealing cover center rubber plug really can significantly simplify the operation that cell is added, reduces and pollutes
It may.But the leakproofness of sealing cover center rubber plug is insufficient, is easy leakage, and the culture medium in centrifuge tube is easy and outside air
Contact, causes culture medium pH value to change, causes cell that can not expand growth, influence subsequent experimental or detection.
Utility model content
The utility model problem to be solved is to provide a kind of cell culture centrifuge tube, has leakproofness good and is easy to add
Sample-adding originally, and can cultivate the advantages of cell is with harvest cell simultaneously, and the utility model solves the technology in the following way and asks
Topic: a kind of cell culture centrifuge tube, including tube body cover the sealing cover at body open, and the sealing lid top wall is equipped with and opens
Hole, the aperture are embedded with soft plug, and the soft plug includes the lower planchet for extending to the roof lower surface, the lower planchet
It is compressed when sealing cover covers tightly tube body by the open end of tube body, by using the soft plug knot that can resist body open
Structure, further improves sealing performance compared with prior art, when use by syringe needle penetrate it is soft fill in capable sample-adding, avoid secondary
It pollutes, soft plug can also be carried out self-styled by own resilient after sample-adding, prevent secondary pollution while capable of being effectively sealed.
As a kind of preferred embodiment of the utility model, the soft plug further includes upper rotary table, and lower planchet is connected to
The upper rotary table bottom, circumferentially arranged with groove on the upper rotary table side wall, the aperture inner wall, which is equipped with, is adapted to the groove
Protrusion.
As a kind of preferred embodiment of the utility model, the lower surface of the protrusion and the roof lower surface of sealing cover
It flushes.
As a kind of preferred embodiment of the utility model, the side wall of the upper rotary table is mutually be bonded between aperture inner wall,
It is mutually be bonded between the upper surface of the lower planchet and sealing lid top wall, soft plug abjection aperture when preventing from puncturing.
As a kind of preferred embodiment of the utility model, the upper rotary table is equipped with sample-adding pit, the lower planchet
Lower end surface be equipped with the plug that be adapted to the inboard wall of tube body, the lower end surface of the plug be it is conical, enable to soft
The certain thickness of maintenance is filled in keep good self-styled effect.
As a kind of preferred embodiment of the utility model, the sample-adding pit is recessed disc or hemisphere face.
As a kind of preferred embodiment of the utility model, the sealing cover includes roof and surrounds in ceiling edge
The peripheral wall of lower section is connected through a screw thread between the peripheral wall medial surface and tube body top outside.
As a kind of preferred embodiment of the utility model, the soft plug is the rubber stopper of butyl rubber material, tool
There is good elasticity to carry out self-styled raising leakproofness to pin hole.
To sum up, using the cell culture centrifuge tube, have the advantages that volume is occupied small and used easy.
Detailed description of the invention
The utility model is further described below in conjunction with attached drawing:
Fig. 1 is the cross-sectional view of the utility model；
Fig. 2 is the cross-sectional view of sealing cover in the utility model；
Fig. 3 is the top view of sealing cover in the utility model；
Fig. 4 is the side view of the utility model middle tube body；
Fig. 5 is the cross-sectional view of present technology；
Wherein: 100- sealing cover, 200- tube body, the soft plug of 300-, 101- aperture, 102- protrusion, 103- screw thread, on 301-
Rotary table, planchet under 302-, 303- plug, 304- are loaded pit, 305- groove.
Specific embodiment
The utility model is further elaborated below by way of specific embodiment:
As shown in Figures 1 to 3, a kind of cell culture centrifuge tube, including sealing cover 100 and tube body 200, sealing cover 100 and pipe
It is connected through a screw thread between body 200,100 roof of sealing cover is equipped with aperture 101, and aperture 101 is embedded with soft plug 300, this is soft
Plug 300 includes upper rotary table 301 and lower planchet 302, and the side wall of upper rotary table 301 circumferentially opens up fluted 305,101 inner wall of aperture
It is equipped with the protrusion 102 for being adapted to the groove, thus soft plug 300 is fastened in aperture 101, which has been paved with close
The lower surface of 100 roofs is covered, in this way when sealing cover 100 is tightened on tube body 200, lower planchet 302 can resist tube body 200
Open end, since lower planchet 302 is soft material, lower planchet 302 when screwing, deformation occurs with fill up lower planchet 302 with
Gap between tube body 200 further functions as sealing effect, in use, passing through the soft plug 300 by syringe needle connects cell
It kind can still keep self-styled by own resilient in pipe, extracting after syringe needle soft plug 300, will not allow liquid outflow that will not allow outer
Boundary's gas and bacterium colony penetrate into, and reduce the contaminated possibility of sample, and the centrifuge tube of leakproofness compared to the prior art has
It is significant to improve.
The lower surface of the protrusion 102 is flushed with roof lower surface, and upper surface of rotary table 301 and roof upper surface are neat on this
It is flat, it is mutually be bonded between rotary table 301 and 101 inner wall of aperture on this in order to further increase the installation fastness of soft plug 300, under this
Planchet 302 is mutually be bonded with roof lower surface, the soft plug 300 in puncture process can be prevented to be detached from sealing covers 100, in order into one
Step improves leakproofness, and the lower surface of lower planchet 302 is equipped with the plug 303 of adaptation 200 inner wall of tube body to further increase sealing effect
Fruit.
For the ease of syringe needle insertion, upper rotary table 301 be equipped with sample-adding pit 304, sample-adding pit 304 be recessed disc or
Hemisphere face, sample-adding pit 304 is set to the center position of upper rotary table 301, corresponding, and the lower end surface of the plug 303 is cone knot
Structure, so that it is enough abundant to maintain certain thickness that can have between sample-adding pit 304 and the lower surface of the soft plug 300
Measure from blockade needle pass through after pin hole.
The soft plug 300 fills for that can seal to be loaded by the contraction of itself using the rubber stopper of butyl rubber material
The pin hole set is realized the isolation of culture solution and outside air in managing, is avoided pollution.
As shown in figure 4, specifically, the sealing cover 100 includes roof and is surrounded in the peripheral wall of roof lower surface edge, week
It is connected through a screw thread between 200 top outside of medial surface and tube body of wall, 200 top of tube body is cylindrical, and bottom is rounding
Taper is to be also able to maintain certain liquid level depth when sample is less, between the bottom surface diameter and upper bottom surface diameter of 200 lower part of tube body
Drafting angle beThe volume of tube body 200 is 15ml.
In summary, using the cell culture centrifuge tube, has and be not easily susceptible to secondary pollution and leakproofness is compared
Product under the prior art more preferably the advantages of.
But those of ordinary skill in the art it should be appreciated that more than embodiment be intended merely to illustrate this
Utility model, and being not used as the restriction to the utility model, if in the spirit of the utility model, to
Variation, the modification of the upper embodiment will all be fallen in the Claims scope of the utility model.
Claims (8)
1. a kind of cell culture centrifuge tube, it is characterised in that: including tube body (200), cover the sealing cover in tube body (200) opening
(100), sealing cover (100) roof is equipped with aperture (101), and the aperture (101) is embedded with soft plug (300), described
Soft plug (300) has the lower planchet (302) for extending to the roof lower surface, and the lower planchet (302) is in sealing cover (100)
It is compressed when covering tightly tube body (200) by the open end of tube body (200).
2. cell culture centrifuge tube described in accordance with the claim 1, the soft plug (300) also has upper rotary table (301), lower circle
Pad (302) is connected to upper rotary table (301) bottom, circumferentially arranged with groove (305), institute on upper rotary table (301) side wall
It states aperture (101) inner wall and is equipped with the protrusion (102) for being adapted to the groove (305).
3. cell culture centrifuge tube according to claim 2, it is characterised in that: the lower surface of the protrusion (102) with it is close
The roof lower surface of capping (100) flushes.
4. cell culture centrifuge tube according to claim 2, it is characterised in that: the side wall of the upper rotary table (301) with open
It is mutually bonded between hole (101) inner wall, it is mutually be bonded between the upper surface and sealing cover (100) roof of the lower planchet (302).
5. cell culture centrifuge tube according to claim 4, it is characterised in that: the upper rotary table (301) is equipped with sample-adding
Pit (304), the lower end surface of the lower planchet (302) are equipped with the plug (303) being adapted to the tube body (200) inner wall, institute
The lower end surface of plug (303) is stated as cone.
6. cell culture centrifuge tube according to claim 5, it is characterised in that: sample-adding pit (304) is recessed
Disc or hemisphere face.
7. cell culture centrifuge tube described in accordance with the claim 1, it is characterised in that: the sealing cover (100) include roof with
And peripheral wall below ceiling edge is surrounded, it is connected between the peripheral wall medial surface and tube body top outside by screw thread (103).
8. cell culture centrifuge tube described in accordance with the claim 1, it is characterised in that: the soft plug (300) is butyl rubber
The rubber stopper of material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821363280.2U CN208857309U (en) | 2018-08-23 | 2018-08-23 | A kind of cell culture centrifuge tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821363280.2U CN208857309U (en) | 2018-08-23 | 2018-08-23 | A kind of cell culture centrifuge tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208857309U true CN208857309U (en) | 2019-05-14 |
Family
ID=66415455
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821363280.2U Active CN208857309U (en) | 2018-08-23 | 2018-08-23 | A kind of cell culture centrifuge tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208857309U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112619718A (en) * | 2020-11-17 | 2021-04-09 | 浙江省海洋水产研究所 | Exempt from to uncap centrifuging tube |
-
2018
- 2018-08-23 CN CN201821363280.2U patent/CN208857309U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112619718A (en) * | 2020-11-17 | 2021-04-09 | 浙江省海洋水产研究所 | Exempt from to uncap centrifuging tube |
| CN112619718B (en) * | 2020-11-17 | 2022-06-14 | 浙江省海洋水产研究所 | Exempt from to uncap centrifuging tube |
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| GR01 | Patent grant |