CN219972325U - Culture device for dendritic cells - Google Patents

Abstract

The utility model discloses a culture device for dendritic cells, which comprises a culture bottle, wherein an opening of the culture bottle is connected with a switching part, a liquid inlet channel and a liquid outlet channel are formed in the switching part, ball valves for simultaneously closing and opening the liquid inlet channel and the liquid outlet channel are arranged in the switching part, the upper end of the switching part is connected with a sealing bottle cap, the sealing bottle cap is connected with a liquid inlet pipe and a liquid outlet pipe, the two lower ends of the sealing bottle cap are respectively inserted into the liquid inlet channel and the liquid outlet channel, the lower end of the liquid outlet channel is connected with a liquid outlet inner pipe, and the liquid inlet pipe and the liquid outlet pipe which are inserted into the liquid inlet channel and the liquid outlet channel are matched with the ball valves which are arranged in the switching part and seal the bottle cap, so that equipment for supplying culture liquid and removing nutrient liquid can be conveniently disconnected, and meanwhile, when the equipment is disconnected, good culture environment in the bottle can be kept by closing the ball valves, and vibration or transfer preservation can be conveniently carried out.

Description

Culture device for dendritic cells

Technical Field

The utility model belongs to the technical field of cell culture devices, and particularly relates to a dendritic cell culture device.

Background

Dendritic cells, which are the most powerful antigen presenting cells so far, are named for the many dendritic or pseudopodiform projections at maturity, often referred to as "natural adjuvants", have become a natural vehicle for antigen transmission, have both immune response and immune tolerance functions, and play an important role in maintaining immune balance.

The preservation of dendritic cells requires dendritic cells under low temperature conditions, and the dendritic cells are likely to adhere to the inner wall of the culture dish, and in this case, the dendritic cells in the culture dish often need to be vibrated.

In order to prevent the external environment from influencing the culture of dendritic cells, usually, very high clean conditions are needed in a culture dish, so a closed culture system for dendritic cells is generally adopted for a device for culturing the dendritic cells, for example, a closed culture system for the dendritic cells disclosed in Chinese patent publication No. CN214655015U comprises a cell culture bottle main body and a sterile filter, the right end of the cell culture bottle main body is connected with the sterile filter, the right end of the cell culture bottle main body is connected with a guide pipe, the other end of the guide pipe is connected with a sample inlet, one side of the sample inlet is fixedly connected with a separating tube, the right side of the guide pipe is respectively connected with a culture medium bag I, a culture medium bag II, a washing solution bag and a waste solution collecting bag, and the culture medium bag and the sterile filter and the waste solution collecting bag can be added and replaced in the culture bottle main body under the closed condition in a matched manner.

Disclosure of Invention

Aiming at the problems, the utility model provides a dendritic cell culture device, which well solves the problem of inconvenient injection caused by unobvious mouse veins in the prior art.

In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows: the utility model provides a culture apparatus of dendritic cell, includes the blake bottle, the opening part of blake bottle is connected with the switching portion, inlet channel and drain passageway have been seted up in the switching portion, be provided with the ball valve that closes simultaneously and open inlet channel and drain passageway in the switching portion, the upper end of switching portion is connected with sealed bottle lid, sealed bottle lid is connected with two lower extreme and inserts feed liquor pipe and drain pipe in inlet channel and the drain passageway respectively, the lower extreme of drain passageway is connected with out the drain inner tube, can close inlet channel and drain passageway through the ball valve, keeps the bottle in-culture environment when breaking off and feed liquor pipe transfer line connection.

Furthermore, the lower end of the liquid outlet inner tube extends and approaches the bottom of the culture flask, so that the culture solution can be conveniently discharged by extraction.

Further, the sealing bottle cap comprises a cap body in threaded connection with the outer wall of the upper end of the switching part and a sealing base plate arranged in the cap body, the liquid inlet pipe is integrally connected with the liquid outlet pipe and the sealing base plate, a passage opening is formed in the cap body, the upper ends of the liquid inlet pipe and the liquid outlet pipe penetrate through the passage opening, and the liquid inlet pipe and the liquid outlet pipe are bonded with the cap body through the sealing base plate to form sealing.

Furthermore, the sealing ring I is arranged at the joint part of the upper side surface of the sealing base plate and the inner wall of the cover body, so that the sealing effect is further improved.

Further, the upper end outer wall of blake bottle and the lower extreme threaded connection of switching portion, the below of switching portion is connected with stretches into the blake bottle in with the bottle's inner wall laminating mutually, reduces the liquid leakage through stopper and blake bottle cooperation.

Further, the joint surface of the inner wall of the bottle plug and the inner wall of the culture bottle is provided with a second sealing ring, so that the sealing effect is further improved.

Furthermore, the culture bottle is made of glass, so that vibration is facilitated, and dendritic cell wall adhesion is prevented.

Furthermore, the culture bottle is a conical bottle, so that the shake action is convenient to hold.

Compared with the prior art, the utility model has the following beneficial effects:

according to the utility model, the ball valves which are arranged in the switching part and can close the liquid inlet channel and the liquid outlet channel are matched with the liquid inlet pipe and the liquid outlet pipe which are arranged on the sealing bottle cover and are inserted into the liquid inlet channel and the liquid outlet channel, so that the equipment for supplying culture solution and removing nutrient solution can be conveniently disconnected, and meanwhile, when the equipment is disconnected, the ball valves can be closed to keep a good culture environment in the bottle, and vibration or transfer preservation can be conveniently carried out.

Drawings

FIG. 1 is a schematic cross-sectional view of the present utility model;

fig. 2 is an enlarged schematic view of the structure of the portion a in fig. 1.

In the figure: 1. a culture bottle; 2. a switching part; 3. a liquid inlet channel; 4. a liquid outlet channel; 5. a ball valve; 6. a liquid inlet pipe; 7. a liquid outlet pipe; 8. a liquid outlet inner tube; 9. a cover body; 10. a sealing backing plate; 11. a passage opening; 12. a first sealing ring; 13. a bottle stopper; 14. and a second sealing ring.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1 and 2, a dendritic cell culture apparatus comprises a culture bottle 1, an opening of the culture bottle 1 is connected with a switching part 2, a liquid inlet channel 3 and a liquid outlet channel 4 which are arranged side by side are longitudinally arranged in the switching part 2, a ball valve 5 is arranged in the switching part 2, the ball valve 5 comprises a valve core and a rotary switch fixedly connected with the valve core, two channel holes which are respectively matched with the liquid inlet channel 3 and the liquid outlet channel 4 are formed in the valve core, the upper end of the switching part 2 is connected with a sealing bottle cap, two lower ends of the sealing bottle cap are respectively inserted into a liquid inlet pipe 6 and a liquid outlet pipe 7 in the liquid inlet channel 3 and the liquid outlet channel 4, and the lower end of the liquid outlet channel 4 is integrally connected with a liquid outlet inner pipe 8.

In this embodiment, the lower end of the liquid outlet inner tube 8 extends and is close to the bottom of the flask 1.

In this embodiment, the sealing bottle cap comprises a cap body 9 and a sealing pad plate 10 arranged in the cap body 9, the cap body 9 and the sealing pad plate 10 are all round, the sealing pad plate 10 can rotate in the cap body 9, external threads are arranged on the upper portion of the switching portion 2, the cap body 9 is provided with internal threads matched with the external threads on the upper portion of the switching portion 2, the cap body 9 is in threaded connection with the switching portion 2, the liquid inlet pipe 6 and the liquid outlet pipe 7 are integrally connected with the sealing pad plate 10, a passage opening 11 is formed in the cap body 9, and the upper ends of the liquid inlet pipe 6 and the liquid outlet pipe 7 penetrate through the passage opening 11.

In this embodiment, a first sealing groove is formed on the upper side surface of the sealing pad 10, and a first sealing ring 12 is installed in the first sealing groove.

In this embodiment, the external screw thread has been seted up to the outer wall of blake bottle 1, the internal screw thread with the outer wall external screw thread complex of blake bottle 1 has been seted up to the lower extreme of switching portion 2, blake bottle 1 and switching portion 2 threaded connection, the below of switching portion 2 an organic whole is connected with stretches into in the blake bottle 1 with the bottle plug 13 that the inner wall of blake bottle 1 laminated mutually.

In this embodiment, a second sealing groove is formed in the side surface of the bottle stopper 13, and a second sealing ring 14 is installed in the second sealing groove.

In this embodiment, the culture flask 1 is a glass conical flask.

When the culture medium feeding device is used, the liquid inlet pipe 6 is connected with the culture medium feeding device, the liquid outlet pipe 7 is connected with the waste liquid collecting device, the liquid inlet channel 3 and the liquid outlet channel 4 are in a communication state through the rotary ball valve 5, new culture medium can be supplemented into the culture bottle 1 through the liquid inlet pipe 6 at the moment, meanwhile, culture medium which needs to be replaced in the culture bottle 1 can be extracted through the communicated liquid outlet inner pipe 8, the liquid outlet channel 4 and the liquid outlet pipe 7, when dendritic cells in the culture bottle 1 need to be oscillated or transferred, the ball valve 5 is closed, the liquid inlet channel 3 and the liquid outlet channel 4 are in a closed state, the upper end of the switching part 2 does not squeeze the sealing base plate 10 any more through the rotary cover body 9, the liquid inlet pipe 6 and the liquid outlet pipe 7 are pulled out, the connection between the culture bottle 1 and the switching part 2 and the culture medium feeding device and the waste liquid collecting device is disconnected, and a good closed culture environment can be kept in the culture bottle 1 at the moment.

Although the present utility model has been described with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements and changes may be made without departing from the spirit and principles of the present utility model.

Claims (8)

1. A culture apparatus for dendritic cells, comprising a culture flask, characterized in that: the opening part of blake bottle is connected with the switching portion, inlet channel and drain channel have been seted up in the switching portion, be provided with the ball valve that closes simultaneously and open inlet channel and drain channel in the switching portion, the upper end of switching portion is connected with sealed bottle lid, sealed bottle lid is connected with two lower extreme and inserts feed liquor pipe and drain pipe in inlet channel and the drain channel respectively, the lower extreme of drain channel is connected with out the liquid inner tube.

2. The culture device of dendritic cells according to claim 1, wherein: the lower end of the liquid outlet inner tube extends and approaches the bottom of the culture flask.

3. The culture device of dendritic cells according to claim 1, wherein: the sealing bottle cap comprises a cap body in threaded connection with the outer wall of the upper end of the adapter part and a sealing base plate arranged in the cap body, the liquid inlet pipe and the liquid outlet pipe are integrally connected with the sealing base plate, a passage opening is formed in the cap body, and the upper ends of the liquid inlet pipe and the liquid outlet pipe penetrate through the passage opening.

4. A culture device for dendritic cells according to claim 3, characterized in that: and a first sealing ring is arranged at the joint part of the upper side surface of the sealing backing plate and the inner wall of the cover body.

5. The culture device of dendritic cells according to claim 1, wherein: the upper end outer wall of blake bottle and the lower extreme threaded connection of switching portion, the below of switching portion is connected with stretches into the blake bottle in the inner wall laminating mutually with the blake bottle.

6. The culture device for dendritic cells according to claim 5, wherein: and a second sealing ring is arranged on the joint surface of the bottle plug and the inner wall of the culture bottle.

7. The culture device of dendritic cells according to claim 1, wherein: the culture bottle is made of glass.

8. The culture device of dendritic cells according to claim 1, wherein: the culture bottle is a conical bottle.