CN201678676U - Nesting type sperm separation and screening tube - Google Patents
Nesting type sperm separation and screening tube Download PDFInfo
- Publication number
- CN201678676U CN201678676U CN 201020176823 CN201020176823U CN201678676U CN 201678676 U CN201678676 U CN 201678676U CN 201020176823 CN201020176823 CN 201020176823 CN 201020176823 U CN201020176823 U CN 201020176823U CN 201678676 U CN201678676 U CN 201678676U
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- inner sleeve
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The utility model discloses a nesting type sperm separation and screening tube, which comprises an outer tube, an inner tube is mounted on the bottom of the outer tube, one end of the inner tube is in the outer tube, the other end of the inner tube is outside the outer tube, and the outer tube is provided with a discharge tube, on which a swinging valve is mounted. Since the nesting type sperm separation and screening tube is provided with the outer tube and the inner tube, semen is placed in the inner tube, nutrient solution is injected in the outer tube, the level of the raw semen in the inner tube cannot be interfered when the nutrient solution containing high-activity sperms is extracted from the outer tube, consequently, the raw semen component cannot be sucked into supernatant, and thereby the quality of screened sperms is increased. The utility model also has the advantages of simple and compact structure, low fabrication cost and easy use.
Description
Technical field
The utility model relates to a kind of inner sleeve type separation screening sperm tube.
Background technology
At present, medical field often adopts the high sperm of upper reaches method screening vigor, be used for artificial fertilization, to solve the infertile problem of some patient, but, the utensil of upper reaches method screening sperm has the following disadvantages: the utensil of upper reaches method screening sperm is the Fahrenheit pipe, when extraction contains the nutritive medium of high motile sperm in the Fahrenheit pipe, because the Fahrenheit pipe is the single tube structure, therefore, inevitably former seminal fluid is also drawn up, weak sperm or the dead essence of refining, vigor in the former seminal fluid sneaked in the nutritive medium, reduced the concentration of high motile sperm, the quality of influence screening sperm.
Summary of the invention
The purpose of this utility model has provided a kind of inner sleeve type separation screening sperm tube, and it can prevent from when extraction contains high vigor nutritive medium former seminal fluid together to be drawn effectively, can improve the quality of sperm screening.
The purpose of this utility model is achieved through the following technical solutions: inner sleeve type separation screening sperm tube, comprise outer tube, and pipe in install the outer tube bottom, interior Guan Yiduan is in outer tube, and the other end is outside outer tube; Drain pipe is set on the outer tube, the valve of outwarding winding is installed on the drain pipe.
For further realizing the purpose of this utility model realizing by the following technical solutions: on the valve of outwarding winding pipe connecting is installed, is installed on the pipe connecting and get spermaduct.Outer tube top mounting pipe lid.Circular groove is offered at the top of pipe lid, triangular groove is offered in the bottom of pipe lid, triangular groove communicates with circular groove, the knob rotating disk is arranged in the circular groove, knob rotating disk top is provided with the knob handle, and knob rotating disk bottom is provided with the triangle steady arm, triangle steady arm and triangular groove running fit, on the knob rotating disk liquid-inlet pipe is installed, the bottom of liquid-inlet pipe is positioned at outer tube.The periphery of liquid-inlet pipe is installed annular first magnetite and sealing-ring, and first magnetite and sealing-ring are positioned at outer tube, and liquid-inlet pipe lower end tube wall is provided with leakage fluid dram; Liquid discharge pipe is installed in the outer tube bottom, and the upper end of liquid discharge pipe links to each other with liquid-inlet pipe, and stretch out outside the outer tube lower end of liquid discharge pipe; Annular second magnetite is installed at the top of liquid discharge pipe.First boss is set on the inwall of liquid discharge pipe, the diaphragm seal and first spring are installed in the liquid discharge pipe, the lower end of first spring contacts with first boss, and diaphragm seal is installed in the upper end of first spring, and diaphragm seal contacts with second magnetite.Outer tube bottom mounting seat.The valve of outwarding winding is connected and composed by the valve body of outwarding winding, handwheel, valve shaft, disk and rubber ring, on the valve body of outwarding winding valve shaft is installed, and an end of valve shaft is installed handwheel, and the other end of valve shaft is installed disk, and disk is positioned at the valve body of outwarding winding, disk periphery rubber ring.Get the spermaduct top rubber plug is installed, get on the sidewall of spermaduct vapor pipe is set, air valve is installed on the vapor pipe.Air valve is by air valve body, spool and push away handle and connect and compose, offer venting port on the air valve body sidewall, the inwall of air valve body is provided with annular boss and orienting lug, second spring and spool are installed in the air valve body, one end of spool passes outer installation of air valve body and pushes away handle, be provided with ventilating pit in the spool, offer guide path on the sidewall of spool, guide path cooperates with orienting lug.
Positively effect of the present utility model is: it has outer tube and interior pipe, place seminal fluid in the interior pipe, the nutritive medium of annotating in the outer tube, when containing the nutritive medium of high motile sperm by the outer tube extraction, the interior former seminal fluid liquid level of pipe is interference-free in can making, prevent that when extraction contains the nutritive medium of high motile sperm former seminal fluid component portion is inhaled in the liquid of upstream, improved the quality of screening sperm.The utility model also has advantage simple and compact for structure, cheap for manufacturing cost and easy to use.
Description of drawings
Fig. 1 is the structural representation of inner sleeve type separation screening sperm tube; Fig. 2 is that the A of Fig. 1 is to structural representation; Fig. 3 is the local structure for amplifying synoptic diagram of the I of Fig. 1; Fig. 4 is the local structure for amplifying synoptic diagram of the II of Fig. 1; Fig. 5 for the B of Fig. 4 to looking the structure for amplifying synoptic diagram; Fig. 6 is the local structure for amplifying synoptic diagram of the III of Fig. 1.
Reference numeral: 1 knob handle, 2 knob rotating disks 3 pipe covers 4 triangle locators, 5 outer tubes, 6 bases, 7 feed tubes, 8 inner tubes, 9 drain pipes 10 valve 11 tube connectors 12 of outwarding winding to be got spermaduct 13 blast pipes 14 air valves 15 rubber stoppers 16 liquid outlets 17 diaphragm seals 18 first springs 19 first boss 20 discharging tubes 21 first magnetites 22 sealing rings 23 second magnetites 24 valve shafts 25 passages 26 disks 27 rubber rings 28 handwheels 29 annular boss 30 second springs 31 exhaust outlets 32 guide pads 33 and pushes away handle 34 spools 35 circular grooves 36 valve body 37 air valve bodies 38 triangular grooves 39 gathering sills 40 annular chambers of outwarding winding.
Embodiment
Inner sleeve type separation screening sperm tube described in the utility model as shown in Figure 1, comprises outer tube 5, pipe 8 in install outer tube 5 bottoms, interior pipe 8 one ends in outer tube 5, the other end outside outer tube 5, looping chamber 40 between outer tube 5 and the interior pipe 8; Drain pipe 9 is set on the outer tube 5, and drain pipe 9 communicates with annular chamber 40, and the valve 10 of outwarding winding is installed on the drain pipe 9.Interior pipe 8 is used to place seminal fluid, and outer tube 5 is used to the sperm nutrient solution of annotating.
During use, pipe 8 in earlier seminal fluid being injected, the upper surface of pipe 8 promptly can not be filled it up with seminal fluid in the seminal fluid liquid level should be lower than, and 2/3rds places of pipe 8 get final product in usually seminal fluid being added to; In annular chamber 40, inject sperm nutrient solution again, when in the nutritive medium liquid level equals, managing 8 upper surfaces, continue to inject sperm nutrient solution, manage in 8, till being full of in sperm nutrient solution injects; Then, inner sleeve type separation screening sperm tube is positioned over hatches 40-60 minute in the incubator, make high motile sperm upstream to sperm nutrient solution; Open the valve 10 of outwarding winding at last, the nutritive medium that contains high motile sperm is flowed out by outer tube 5.When containing the sperm nutrient solution of high motile sperm with the extraction of inner sleeve type separation screening sperm tube described in the utility model, pipe 8 interior former seminal fluid liquid levels are interference-free in can guaranteeing, prevent other formed elementss in the refining, for example white corpuscle, epithelial cell, bacterium etc., and weak sperm or the Necrospermia of motility sneak in the extracting solution, thereby can improve the effect of sperm screening.
Extract the nutritive medium that contains high motile sperm for convenience, as shown in Figure 1, pipe connecting 11 is installed on the valve 10 of outwarding winding, install on the pipe connecting 11 and get spermaduct 12.Open the valve 10 of outwarding winding, the sperm nutrient solution that contains high motile sperm is flowed directly into through outward winding valve 10 and pipe connecting 11 by outer tube 5 to be got in the spermaduct 12.
For in the process of hatching, not being subjected to outside contamination, as shown in Figure 1, outer tube 5 top mounting pipe lids 3.
Can under the environment of comparatively sealing, carry out in order to make notes seminal fluid and sperm nutrient solution process, prevent as far as possible that in injection process seminal fluid or sperm nutrient solution are by external environmental, as depicted in figs. 1 and 2, pipe covers 3 top offers circular groove 35, pipe covers 3 bottom offers triangular groove 38, the diameter of circular groove 35 is greater than the circumscribed circle diameter of triangular groove 38, triangular groove 38 communicates with circular groove 35, knob rotating disk 2 is arranged in the circular groove 35, knob rotating disk 2 tops are provided with knob handle 1, knob rotating disk 2 bottoms are provided with triangle steady arm 4, triangle steady arm 4 and triangular groove 38 running fit, liquid-inlet pipe 7 is installed on the knob rotating disk 2, and the bottom of liquid-inlet pipe 7 is positioned at outer tube 5.In order to ensure triangle steady arm 4 being separated with triangular groove 38 so that knob rotating disk 2 can be rotated, the thickness of triangle steady arm 4 and knob rotating disk 2 close the degree of depth that equals circular groove 35.
When injecting seminal fluid, mention knob handle 1, by knob rotating disk 2 with triangle steady arm 4 by pulling out in the triangular groove 38, turning knob rotating disk 2 is threaded to interior 8 tops, i.e. the b position of managing with liquid-inlet pipe 7, again triangle steady arm 4 is embedded in the triangular groove 38, promptly finish the location work of annotating seminal fluid, at this moment, can inwardly manage and inject seminal fluid in 8.After finishing the seminal fluid injection, when needing to annotate sperm nutrient solution, mention knob handle 1, and turning knob rotating disk 2, make liquid-inlet pipe 7 be threaded to a position or c position, triangle steady arm 4 is embedded in the triangular groove 38, at this moment, liquid-inlet pipe 7 is positioned at annular chamber 40, can inject sperm nutrient solution in outer tube 5.
In order further to guarantee to screen the quality of sperm, prevent that liquid-inlet pipe 7 from adding seminal fluid after, add again in the process of sperm nutrient solution, the residual seminal fluid in the liquid-inlet pipe 7 together enter in the annular chamber 40 with sperm nutrient solution.As shown in figures 1 and 3, the periphery of liquid-inlet pipe 7 can be installed annular first magnetite 21 and sealing-ring 22, the first magnetites 21 and sealing-ring 22 and is positioned at outer tube 5, and liquid-inlet pipe 7 lower end tube walls are provided with leakage fluid dram 16; Liquid discharge pipe 20 is installed in outer tube 5 bottoms, and the upper end of liquid discharge pipe 20 links to each other with the lower end of liquid-inlet pipe 7, and stretch out outside the outer tube 5 lower end of liquid discharge pipe 20; Annular second magnetite 23 is installed at the top of liquid discharge pipe 20.When adding seminal fluid, mention knob handle 1 and turning knob rotating disk 2 liquid-inlet pipe 7 is separated with liquid discharge pipe 20, and be positioned at the top of pipe 8, be i.e. the b position; Clean liquid-inlet pipe 7 after adding seminal fluid, mentioning knob handle 1 rotates and knob rotating disk 2, make liquid-inlet pipe 7 be threaded to liquid discharge pipe 20 tops, it is a position, triangle steady arm 4 is embedded in the triangular groove 38, liquid-inlet pipe 7 lower ends are inserted in the liquid discharge pipe 20, at this moment, first magnetite 21 on the liquid-inlet pipe 7 and second magnetite 23 on the liquid discharge pipe 20 attract each other and compress sealing-ring 22, sealing-ring 22 can be guaranteed the resistance to air loss between liquid-inlet pipe 7 and the liquid discharge pipe 20, and liquid spills when preventing to clean, and can inject the tube wall that distilled water cleans liquid-inlet pipe 7 this moment in liquid-inlet pipe 7, distilled water flows into liquid discharge pipe 20 through liquid outlet 16, and is discharged outside the outer tube 5 by liquid discharge pipe 20; After clean finishing, mention knob handle 1 turning knob rotating disk 2, liquid-inlet pipe 7 is threaded to the c position, triangle steady arm 4 is embedded in the triangular grooves 38 behind the location, in outer tube 5, inject sperm nutrient solution; After injecting sperm nutrient solution, for guaranteeing sealing in the outer tube 5, should forward liquid-inlet pipe 7 to a position once more, utilize first magnetite 21, second magnetite 23 and sealing-ring 22, make the lower port of liquid-inlet pipe 7 and the upper port sealing of liquid discharge pipe 20, the sperm nutrient solution in guaranteeing in the seminal fluid of pipe 8 and the outer tube 5 is hedged off from the outer world.
For the step that simplifies the operation, increase work efficiency, as shown in Figure 3, first boss 19 is set on the inwall of liquid discharge pipe 20, the diaphragm seal 17 and first spring 18 are installed in the liquid discharge pipe 20, the lower end of first spring 18 contacts with first boss 19, diaphragm seal 17 is installed in the upper end of first spring 18, and diaphragm seal 17 contacts with second magnetite 23.Depress diaphragm seal 17 and can compress second magnetite 23 under the effect of first spring 18, with 23 sealings of second magnetite, the upper port that is about to liquid discharge pipe 20 is stopped up, therefore, after injecting sperm nutrient solution, can need not to rotate liquid-inlet pipe 7 again, utilize liquid-inlet pipe 7 to be connected sealing with liquid discharge pipe 20.When need clean liquid-inlet pipe 7, liquid-inlet pipe 7 can be inserted in the liquid discharge pipes 20, the elastic force that overcomes first spring 18 is depressed diaphragm seal 17 and is got final product.Under the magneticaction of first magnetite 21 and second magnetite 23, can prevent that first spring 18 from pushing liquid-inlet pipe 7 open.
For the ease of placing outer tube 5, as shown in Figure 1, but outer tube 5 bottom mounting seat 6.
The described valve 10 of outwarding winding can be existing ball valve, also can be structure as shown in Figure 4: the valve 10 of outwarding winding be connected and composed by the valve body 36 of outwarding winding, handwheel 28, valve shaft 24, disk 26 and rubber ring 27, outward winding valve shaft 24 is installed on the valve body 36, one end of valve shaft 24 is installed handwheel 28, the other end of valve shaft 24 is installed disk 26, disk 26 is positioned at the valve body 36 of outwarding winding, disk 26 periphery rubber rings 27.The described valve 10 of outwarding winding of this scheme uses more lightly nimble than existing ball-valve structure is more simple, volume is littler, weight is lighter.
For in closed environment, extracting the nutritive medium that contains high motile sperm, guarantee that further the sperm nutrient solution that extracts is not subjected to external pollution, as shown in Figure 1, get spermaduct 12 tops rubber plug 15 is installed, get on the sidewall of spermaduct 12 vapor pipe 13 is set, air valve 14 is installed on the vapor pipe 13.When extraction contains the nutritive medium of high motile sperm, open the valve 10 of outwarding winding earlier, open air valve 14 again, sperm nutrient solution just can enter and get in the spermaduct 12.
As shown in Figure 6, described air valve 14 can be by air valve body 37, spool 34 and push away handle 33 and connect and compose, offer venting port 31 on air valve body 37 sidewalls, the inwall of air valve body 37 is provided with annular boss 29 and orienting lug 32, second spring 30 and spool 34 are installed in the air valve body 37, second spring 30 is between spool 34 and annular boss 29, one end of spool 34 passes air valve body 37 outer installations and pushes away handle 33, be provided with ventilating pit 25 in the spool 34, offer guide path 39 on the sidewall of spool 34, guide path 39 cooperates with orienting lug 32, guide path 39 can make spool 34 rotate relative to air valve body 37 with orienting lug 32, thereby when guaranteeing to extract nutritive medium, ventilating pit 25 can corresponding communicating with venting port 31.When needing in getting spermaduct 12, to inject sperm nutrient solution, push away handle 33, ventilating pit 25 is got final product with venting port 31 corresponding communicating.
The utility model not technology contents of detailed description is known technology.
Claims (10)
1. inner sleeve type separation screening sperm tube is characterized in that: comprise outer tube (5), and pipe (8) in install outer tube (5) bottom, interior pipe (8) one ends are in outer tube (5), and the other end is outside outer tube (5); Drain pipe (9) is set on the outer tube (5), and drain pipe (9) is gone up the valve (10) of outwarding winding is installed.
2. inner sleeve type separation screening sperm tube according to claim 1 is characterized in that: the valve of outwarding winding (10) is gone up pipe connecting (11) is installed, and pipe connecting (11) is gone up to install and got spermaduct (12).
3. inner sleeve type separation screening sperm tube according to claim 1 and 2 is characterized in that: outer tube (5) top mounting pipe lid (3).
4. inner sleeve type separation screening sperm tube according to claim 3, it is characterized in that: circular groove (35) is offered at the top of pipe lid (3), triangular groove (38) is offered in the bottom of pipe lid (3), triangular groove (38) communicates with circular groove (35), knob rotating disk (2) is arranged in the circular groove (35), knob rotating disk (2) top is provided with knob handle (1), knob rotating disk (2) bottom is provided with triangle steady arm (4), triangle steady arm (4) and triangular groove (38) running fit, knob rotating disk (2) is gone up liquid-inlet pipe (7) is installed, and the bottom of liquid-inlet pipe (7) is positioned at outer tube (5).
5. inner sleeve type separation screening sperm tube according to claim 4, it is characterized in that: the periphery of liquid-inlet pipe (7) is installed annular first magnetite (21) and sealing-ring (22), first magnetite (21) and sealing-ring (22) are positioned at outer tube (5), and liquid-inlet pipe (7) lower end tube wall is provided with leakage fluid dram (16); Liquid discharge pipe (20) is installed in outer tube (5) bottom, and the upper end of liquid discharge pipe (20) links to each other with liquid-inlet pipe (7), and stretch out outside the outer tube (5) lower end of liquid discharge pipe (20); Annular second magnetite (23) is installed at the top of liquid discharge pipe (20).
6. inner sleeve type separation screening sperm tube according to claim 5, it is characterized in that: first boss (19) is set on the inwall of liquid discharge pipe (20), diaphragm seal (17) and first spring (18) are installed in the liquid discharge pipe (20), the lower end of first spring (18) contacts with first boss (19), diaphragm seal (17) is installed in the upper end of first spring (18), and diaphragm seal (17) contacts with second magnetite (23).
7. inner sleeve type separation screening sperm tube according to claim 1 is characterized in that: outer tube (5) bottom mounting seat (6).
8. inner sleeve type separation screening sperm tube according to claim 1, it is characterized in that: the valve of outwarding winding (10) is connected and composed by the valve body of outwarding winding (36), handwheel (28), valve shaft (24), disk (26) and rubber ring (27), the valve body (36) of outwarding winding is gone up valve shaft (24) is installed, one end of valve shaft (24) is installed handwheel (28), the other end of valve shaft (24) is installed disk (26), disk (26) is positioned at the valve body of outwarding winding (36), disk (26) periphery rubber ring (27).
9. inner sleeve type separation screening sperm tube according to claim 2 is characterized in that: get spermaduct (12) top rubber plug (15) is installed, get on the sidewall of spermaduct (12) vapor pipe (13) is set, vapor pipe (13) is gone up air valve (14) is installed.
10. inner sleeve type separation screening sperm tube according to claim 9, it is characterized in that: air valve (14) is by air valve body (37), spool (34) and push away handle (33) and connect and compose, offer venting port (31) on air valve body (37) sidewall, the inwall of air valve body (37) is provided with annular boss (29) and orienting lug (32), second spring (30) and spool (34) are installed in the air valve body (37), one end of spool (34) passes outer installation of air valve body (37) and pushes away handle (33), be provided with ventilating pit (25) in the spool (34), offer guide path (39) on the sidewall of spool (34), guide path (39) cooperates with orienting lug (32).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020176823 CN201678676U (en) | 2010-04-30 | 2010-04-30 | Nesting type sperm separation and screening tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020176823 CN201678676U (en) | 2010-04-30 | 2010-04-30 | Nesting type sperm separation and screening tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201678676U true CN201678676U (en) | 2010-12-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201020176823 Expired - Fee Related CN201678676U (en) | 2010-04-30 | 2010-04-30 | Nesting type sperm separation and screening tube |
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| Country | Link |
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| CN (1) | CN201678676U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020237739A1 (en) * | 2019-05-30 | 2020-12-03 | 南京精竞生物科技有限公司 | Microfluidic device for screening high-motility sperms |
-
2010
- 2010-04-30 CN CN 201020176823 patent/CN201678676U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020237739A1 (en) * | 2019-05-30 | 2020-12-03 | 南京精竞生物科技有限公司 | Microfluidic device for screening high-motility sperms |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101222 Termination date: 20140430 |