CN205042500U - Cell centrifuging tube - Google Patents
Cell centrifuging tube Download PDFInfo
- Publication number
- CN205042500U CN205042500U CN201520696702.8U CN201520696702U CN205042500U CN 205042500 U CN205042500 U CN 205042500U CN 201520696702 U CN201520696702 U CN 201520696702U CN 205042500 U CN205042500 U CN 205042500U
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- cell
- pipe lid
- tubular section
- pipe
- cell centrifugation
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Abstract
The utility model discloses a cell centrifuging tube, for the body of a top end opening, the body divide into upper portion pipeline section and lower part pipeline section, the upper portion pipeline section with through reducing pipe section intercommunication between the pipeline section of lower part. The cell centrifuging tube of this kind of structure has easy extraction and distributes in the advantage of centrifuging tube liquid intermediate position cell.
Description
Technical field
The utility model belongs to medical detection apparatus technical field, particularly relates to a kind of centrifuge tube.
Background technology
Centrifugation technique is in bioscience, and particularly biochemistry and molecular biology research field, has obtained applying very widely, and each biochemistry and Molecular Biology Lab will prepare the centrifuge of multiple pattern.Centrifugation technique is mainly used in separation and the preparation of various biological sample, biological sample suspension to be contained in centrifuge tube under High Rotation Speed, due to huge centrifugal action, make the molecule of suspension (precipitation etc. as organelle, large biological molecule) with certain velocity sedimentation, thus be separated with solution.
With some cell, as blood platelet, white blood cell and stem cell, nutrient solution is after centrifuge rotating centrifugal, and cell distribution, in the centre position of centrifuge tube liquid, namely roughly forms upper liquid, intermediate cell layer and lower liquid in centrifuge tube.Intermediate cell layer is skim, and thickness is very little, so be not easy to carry out extracting these cells, during extraction, necessary significant care is careful, otherwise is easy to be drawn into upper liquid or lower liquid because of carelessness.
In addition, when suspension cell will carry out in vitro culture, need nutrient solution to be added in blake bottle, inoculate cell and cultivate.After cell chulture, when collecting cell to carry out follow-up experiment or research, the nutrient solution containing suspension cell must be had in blake bottle again and take out, be put in centrifuge tube centrifugal, could collecting cell.The a large amount of manual operation of these process needs, needs the operating technology of extreme care to avoid cell contamination simultaneously, very inconvenient and be easy to failure.
Utility model content
For this reason, the utility model provides a kind of easy extraction to be distributed in the cell centrifugation pipe of centrifuge tube liquid centre position cell, to overcome the deficiency that prior art exists.
For solving the problems of the technologies described above, the utility model adopts following technical scheme:
A kind of cell centrifugation pipe, is the body of a top end opening, it is characterized in that: described body is divided into upper tubular section and lower tubular section, be communicated with between described upper tubular section and described lower tubular section by undergauge pipeline section.
The inside diameter of described lower tubular section is 15mm, and the height of described lower tubular section is 55-70mm; The inside diameter of described undergauge pipeline section is 8mm, is highly 15mm.
In addition, for convenience of cell chulture operation and avoid cell contaminated, the oral area of described body is also connected with pipe lid, and the center of described pipe lid has soft rubber plug.Soft rubber plug can allow syringe needle puncture, and seeds cells in pipe, but other material or bacterium can not be allowed to pass, and after syringe needle extracts, still can keep sealing, liquid can not be allowed to flow out, other material or bacterium also can not be allowed to pass.
In the utility model detailed description of the invention, described pipe lid is connected with the mouth thread of body, the center of described pipe lid has circular hole, described soft rubber plug is step structure, top is round platform, and bottom is planchet, and described round platform is arranged in the circular hole of described pipe lid, the surrounding of described round platform is bonding with the inwall of described circular hole, and described planchet is bonding with the top lower surface of pipe lid.
For ensure pipe cover screw on body after do not reveal, described planchet is paved with the top lower surface of described pipe lid.
In the detailed description of the invention of utility model, described soft rubber plug and described pipe lid are bonded together by the mode of injection moulding.
Adopt technique scheme, the utility model has the advantage easily extracting and be distributed in centrifuge tube liquid centre position cell, and has and facilitate cell culture operations and avoid the contaminated advantage of cell.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the utility model is described in detail:
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the structural representation of pipe lid.
Detailed description of the invention
As shown in Figure 1, cell centrifugation pipe of the present utility model, comprises body 100 and pipe lid 200.
Wherein, body 100 top end opening, is divided into three sections, and be followed successively by upper tubular section 101, undergauge pipeline section 102 and lower tubular section 103 from top to bottom, upper tubular section 101 is communicated with by undergauge pipeline section 102 with lower tubular section 103.
In the cell centrifugation pipe of concrete a kind of specification, the inside diameter of upper tubular section 101 and lower tubular section 103 is 15mm, and lower tubular section 103 is highly 55-70mm; The inside diameter of undergauge pipeline section 102 is 8mm, is highly 15mm.
By arranging a bit of undergauge at body 100, the cellular layer being in the centre position of centrifuge tube liquid being centrifuged separation is like this just relatively in the past thickening, and extraction cell just becomes and is more prone to like this.
In addition, for convenience of cell chulture operation and avoid cell contaminated, pipe lid 200 covers the oral area at body 100, and pipe lid 200 is threaded connection with the oral area of body 100.
As shown in Figure 2, the center of pipe lid 200 has soft rubber plug 300.Soft rubber plug can allow syringe needle puncture, and seeds cells in pipe, but other material or bacterium can not be allowed to pass, and after syringe needle extracts, still can keep sealing, liquid can not be allowed to flow out, other material or bacterium also can not be allowed to pass.
In the present embodiment, the center of pipe lid 200 has circular hole 201.Soft rubber plug 300 is step structure, and top is the adaptive round platform 301 of circular hole 201 of pipe lid 200, and bottom is the planchet 302 with the top lower surface adaptation of pipe lid 200.Round platform 300 is arranged in the circular hole 201 of pipe lid 200, and the surrounding of round platform 300 is bonding with the inwall of circular hole 201, and planchet 302 is paved with the top lower surface of pipe lid 200 and bonding with the top lower surface of pipe lid 200.Particularly, soft rubber plug 200 and pipe lid 200 are bonded together by the mode of injection moulding.Such structure, is tightened on pipe lid 200 after on body 100, and the oral area of body 100 can be supported on the cushion 302 of soft rubber plug 300, plays sealing function, avoids entering of the leakage of liquid and other material or bacterium.
In addition, as shown in Figure 1, body 100 is also labeled with volume markings 104.Like this, can direct good plan liquid volume, allow user know and add how much liquid.
The utility model in use, first uses body 100 filling cell culture medium in gnotobasis, then by pipe lid 200 screwing hermetic.When needs carry out cell chulture, use the soft rubber plug on the needle pierces pipe lid 200 of syringe, cell is injected in body and cultivates.When cultured cell needs to be separated, be directly placed on centrifuge by this centrifuge tube and carry out rotating centrifugal, after centrifugal, cell is just in time distributed in undergauge pipeline section 102, and has certain thickness, and the extraction of cell is become than being easier to.
Therefore, can be found out by above-mentioned detailed introduction, the utility model has the advantage easily extracting and be distributed in centrifuge tube liquid centre position cell, and has and facilitate cell culture operations and avoid the contaminated advantage of cell.
Claims (7)
1. a cell centrifugation pipe, is the body of a top end opening, it is characterized in that: described body is divided into upper tubular section and lower tubular section, is communicated with between described upper tubular section and described lower tubular section by undergauge pipeline section.
2. cell centrifugation pipe according to claim 1, is characterized in that: the inside diameter of described upper tubular section and described lower tubular section is 15mm, and the height of described lower tubular section is 55-70mm; The inside diameter of described undergauge pipeline section is 8mm, is highly 15mm.
3. cell centrifugation pipe according to claim 1 and 2, is characterized in that: the oral area also pipe lid of described body, the center of described pipe lid has soft rubber plug.
4. cell centrifugation pipe according to claim 3, it is characterized in that: described pipe lid is connected with the mouth thread of body, the center of described pipe lid has circular hole, described soft rubber plug is step structure, top is round platform, and bottom is planchet, and described round platform is arranged in the circular hole of described pipe lid, the surrounding of described round platform is bonding with the inwall of described circular hole, and described planchet is bonding with the top lower surface of pipe lid.
5. cell centrifugation pipe according to claim 4, is characterized in that: described planchet is paved with the top lower surface of described pipe lid.
6. cell centrifugation pipe according to claim 5, is characterized in that: described soft rubber plug and described pipe lid are bonded together by the mode of injection moulding.
7. cell centrifugation pipe according to claim 3, is characterized in that: described body is labeled with volume markings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520696702.8U CN205042500U (en) | 2015-09-10 | 2015-09-10 | Cell centrifuging tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520696702.8U CN205042500U (en) | 2015-09-10 | 2015-09-10 | Cell centrifuging tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205042500U true CN205042500U (en) | 2016-02-24 |
Family
ID=55336118
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520696702.8U Active CN205042500U (en) | 2015-09-10 | 2015-09-10 | Cell centrifuging tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205042500U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106582909A (en) * | 2016-12-30 | 2017-04-26 | 张方亮 | Centrifuge tube and method for preparing platelet-rich plasma by using same |
| CN106979881A (en) * | 2017-05-18 | 2017-07-25 | 桂林医学院 | A kind of centrifuge tube prepared for cell block |
| CN110325280A (en) * | 2017-01-25 | 2019-10-11 | 烟台澳斯邦生物工程有限公司 | The device and method of automation sample treatment for diagnostic purposes |
-
2015
- 2015-09-10 CN CN201520696702.8U patent/CN205042500U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106582909A (en) * | 2016-12-30 | 2017-04-26 | 张方亮 | Centrifuge tube and method for preparing platelet-rich plasma by using same |
| CN110325280A (en) * | 2017-01-25 | 2019-10-11 | 烟台澳斯邦生物工程有限公司 | The device and method of automation sample treatment for diagnostic purposes |
| CN110325280B (en) * | 2017-01-25 | 2022-04-05 | 烟台澳斯邦生物工程有限公司 | Apparatus and method for automated sample processing for diagnostic purposes |
| CN106979881A (en) * | 2017-05-18 | 2017-07-25 | 桂林医学院 | A kind of centrifuge tube prepared for cell block |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |