CN206746829U - A kind of infiltration type centrifuge tube - Google Patents

A kind of infiltration type centrifuge tube Download PDF

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Publication number
CN206746829U
CN206746829U CN201720471143.XU CN201720471143U CN206746829U CN 206746829 U CN206746829 U CN 206746829U CN 201720471143 U CN201720471143 U CN 201720471143U CN 206746829 U CN206746829 U CN 206746829U
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China
Prior art keywords
tube
inner tube
impermeable inner
outer tube
centrifugation
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CN201720471143.XU
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Chinese (zh)
Inventor
李智龙
陈国征
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Shenzhen Kekang Clinical Medicine Research Co.,Ltd.
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Qianhai Shenzhen Precision Biological Science And Technology Co Ltd
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Abstract

Biomedicine experiment Work tool technical field is the utility model is related to, especially a kind of infiltration type centrifuge tube.It includes centrifugation outer tube, impermeable inner tube and will centrifuge outer tube and sealing gag;Impermeable inner tube is the tubular body structure that top and bottom offer the mouth of pipe, and permeable screens are equiped with the upper end of impermeable inner tube, and permeable screens are the trapezoidal bag shaped structure body of axial cross section.The utility model can realize filtration treatment early stage to biological sample solution by the impermeable inner tube and permeable screens set up, the material composition for making particle diameter larger is retained in impermeable inner tube, and other material compositions can be then leaked by permeable screens to centrifugation outer tube, simultaneously the filter area to biological sample solution can be effectively increased using inclined-plane form possessed by the waist edge side wall of permeable screens, so as to effectively improve the centrifugal separating effect to biological sample solution, shorten the run time of the time centrifuged and rotary substrate, reach the effect for reducing energy consumption and improving operating efficiency.

Description

A kind of infiltration type centrifuge tube
Technical field
Biomedicine experiment Work tool technical field is the utility model is related to, especially a kind of infiltration type centrifuge tube.
Background technology
Centrifugation technique has been obtained quite varied in bioscience, particularly biochemistry and molecular biology research field Application, it is mainly used in the separation of various biological samples and preparation, after biological sample suspension is contained in centrifuge tube, passes through Centrifuge tube is set to carry out high speed rotary motion, by huge centrifugal action, to make molecule (such as organelle, life of suspension Thing macromolecular etc.) settled with certain speed, so as to be separated with solution.Existing centrifuge tube when in use, typically Need to only the biological sample solution for needing to separate be imported into centrifuge tube and be closed the lid, then centrifuge tube is placed in and such as centrifuged Rotating separation is carried out on the rotary substrates such as machine, due to not knowing the concrete condition of remaining material remained in cell solution, So when being centrifuged, the long time can be typically set so that solution can be separated more thorough;Thus, Just centrifugal treating easily occur, time-consuming, operating efficiency is low, rotary substrate operates the problems such as time length, energy consumption height.Meanwhile It is unreasonable structure design to be also present in existing centrifuge tube, it is inconvenient for use the defects of.
Utility model content
For above-mentioned the shortcomings of the prior art, the purpose of this utility model is to provide a kind of infiltration type centrifuge tube.
To achieve these goals, the utility model adopts the following technical scheme that:
A kind of infiltration type centrifuge tube, it includes the centrifugation outer tube of top end opening and bottom end closure, the top by centrifugation outer tube Mouthful sleeve is in the impermeable inner tube in the upper end of centrifugation outer tube and will centrifuge the upper end of outer tube and impermeable inner tube and be socketed sealing Gag;The impermeable inner tube is the tubular body structure that top and bottom offer the mouth of pipe, in the upper end of the impermeable inner tube The permeable screens for being used to the push pipe mouth of impermeable inner tube and bottom tube mouth being separated are equiped with, the permeable screens cut for axial direction The trapezoidal bag shaped structure body in face, the port side wall of the permeable screens and the internal perisporium of impermeable inner tube are fixed as one.
Preferably, the permeable screens enclose shaping by composite fibre miillpore filter or polyethersulfone membranes.
Preferably, the impermeable inner tube include press on centrifugation outer tube top ports end face on fixation ring portion and by The bottom surface of fixed ring portion and the interior pipe portion that aftershaping is extended downwardly around the inner ring opening's edge axial direction for fixing ring portion, the fixation The first external screw thread area for being threadedly coupled with sealing gag is provided with the periphery wall of ring portion, the upper end in said inner tube portion The second external screw thread area for being connected with centrifugation external pipe thread is provided with periphery wall, is opened up on the bottom in said inner tube portion Bottom tube mouth of the trapezoidal relief port of an axial cross section as impermeable inner tube, the port side wall of the permeable screens is fixed on Determine on the collar extension perisporium of ring portion, be provided with the periphery wall of the upper end of the centrifugation outer tube for being threadedly coupled with sealing gag The 3rd external screw thread area.
Preferably, it is described centrifugation outer tube upper end internal perisporium on be provided with one be used for block be formed at interior pipe portion outside The closure ring in the gap between perisporium and the internal perisporium for centrifuging outer tube.
Preferably, it is additionally provided with support arm on the periphery wall of the centrifugation outer tube.
Preferably, the sealing gag includes one simultaneously with centrifuging the upper end of outer tube and the upper end screw thread of impermeable inner tube The cannula portion of socket, a clamped through hole is offered on the top wall surface in described sleeve pipe portion, a rubber is equiped with the clamped through hole Sealing-plug, the end face center region of the rubber seal plug in axial direction to after lower recess formed with a ball groove, bottom center Formed with a conical plug being placed in the upper end of impermeable inner tube after region is in axial direction downwardly convex.
Preferably, offer an annular groove on the perisporium of the rubber seal plug, on the hole wall of the clamped through hole formed with One contraposition is embedded in the ring convex in annular groove.
As a result of such scheme, the utility model can be realized to biology by the impermeable inner tube and permeable screens set up Filtration treatment early stage of sample solution, the material composition for making particle diameter larger is retained in impermeable inner tube, and other material compositions It can then leak by permeable screens to centrifugation outer tube, while can have using inclined-plane form possessed by the waist edge side wall of permeable screens Effect increase, so as to effectively improve the centrifugal separating effect to biological sample solution, is contracted to the filter area of biological sample solution The time of short centrifugation and the run time of rotary substrate, reach the effect for reducing energy consumption and improving operating efficiency;Its structure Simple and compact, dismount and easy to maintenance quick, there is very strong practical value and market popularization value.
Brief description of the drawings
Fig. 1 is the cross section structure schematic diagram of the utility model embodiment in the assembled condition;
Fig. 2 is the cross section structure schematic diagram of the utility model embodiment in a disassembled state
Embodiment
Embodiment of the present utility model is described in detail below in conjunction with accompanying drawing, but the utility model can be by right It is required that the multitude of different ways for limiting and covering is implemented.
As depicted in figs. 1 and 2, a kind of infiltration type centrifuge tube that the present embodiment provides, it includes top end opening and bottom is sealed Close centrifugation outer tube 10, by centrifugation outer tube 10 top ports sleeve in centrifugation outer tube 10 upper end in impermeable inner tube 20 and The upper end socket sealing gag 30 of outer tube 10 and impermeable inner tube 20 will be centrifuged;Wherein, impermeable inner tube 20 is that top and bottom are equal Offer the tubular body structure of the mouth of pipe, be equiped with the upper end of impermeable inner tube 20 one be used for the push pipe mouth of impermeable inner tube 20 and The permeable screens 40 that bottom tube mouth is separated, permeable screens 40 are the trapezoidal bag shaped structure body of axial cross section, permeable screens 40 Port side wall and the internal perisporium of impermeable inner tube 10 be fixed as one.In this way, pass through the impermeable inner tube 20 and permeable screens set up 40 can change conventional centrifugal pipe by body+sealing cover combined structure form, when will centrifugation outer tube 10, impermeable inner tube 20 and sealing-plug After the assembling of lid 30 is integrated, puncture processing can be carried out to sealing gag 30 by instruments such as syringes, so as to impermeable inner tube 20 Interior injection biological sample solution, because filtration treatment early stage to biological sample solution can be achieved in the effect of permeable screens 40, make The larger material composition of the particle diameters such as organelle, large biological molecule is retained in impermeable inner tube 20 (i.e.:Permeable screens 40 In superjacent air space), and the less material composition of other particle diameters or liquid can then pass through permeable screens 40 and impermeable inner tube 30 Bottom tube mouth is leaked in centrifugation outer tube 10, because permeable screens 40 are using trapezoidal bag shaped structure is similar to, utilizes permeable screens Inclined-plane form possessed by 40 waist edge side wall can be effectively increased the filter area to biological sample solution;Based on this, due to oozing Filter net 10 can carry out filtration treatment early stage to biological sample solution, so as to effectively improve the centrifugation point to biological sample solution From effect, shorten the run time of the rotary substrates such as time and the centrifuge of centrifugation, reaching reduces energy consumption and raising work Make the purpose of efficiency.
Preferably, to improve the filter effect to biological sample solution, the permeable screens 40 of the present embodiment can root Shaping is enclosed by filter membranes such as composite fibre miillpore filters or polyethersulfone membranes according to concrete condition.
To optimize the structure of whole centrifuge tube to greatest extent, the facility for being dismounted and being safeguarded to building block is improved Property, the impermeable inner tube 20 of the present embodiment include pressing on fixation ring portion 21 on the end face of the top ports of centrifugation outer tube 10 and by The bottom surface of fixed ring portion 21 and the interior pipe portion 22 that aftershaping is extended downwardly around the inner ring opening's edge axial direction for fixing ring portion 21, The first external screw thread area a for being threadedly coupled with sealing gag 30 is provided with the periphery wall of fixed ring portion 21, in interior pipe portion 22 Upper end periphery wall on be provided with for centrifugation the second external screw thread area b that is threadedly coupled of outer tube 10, and interior pipe portion 22 The trapezoidal relief port c of the axial cross section that is opened up on bottom then bottom tube mouths as impermeable inner tube 20, permeable screens 40 Port side wall be fixed on the collar extension perisporium of fixed ring portion 21, and centrifugation outer tube 10 upper end periphery wall on set There is the 3rd external screw thread area d for being threadedly coupled with sealing gag 30.Thus, centrifuge outer tube 10 by the second external screw thread area b with After the thread bush of impermeable inner tube 20 is connected in one, relation can be socketed by centrifuge tube by sealing gag 30 and screw thread between the two again Each building block rapidly assemble and be integrated, and be advantageous to that all parts are quickly disassembled and safeguarded;Meanwhile by In impermeable inner tube 20 bottom tube mouth (i.e.:Relief port c) can be beneficial to warp using bell shape or trapezoidal structural form is similar to The material composition that permeable screens 40 filter is crossed equably to drop onto in centrifugation outer tube 10.
To avoid by the material composition that impermeable inner tube 20 leaks because between intrusion centrifugation outer tube 10 and impermeable inner tube 20 Gap in and influence centrifugal separating effect or be not easy to later stage cleaning and maintenance to part, in the upper end of centrifugation outer tube 10 Internal perisporium on be provided with one be used for block be formed at interior pipe portion 22 periphery wall and centrifugation outer tube 10 internal perisporium between seam The closure ring 11 of gap.
As a preferred scheme, for ease of by whole centrifuge tube be installed in the rotary substrates such as centrifuge or from On vascular stents, support arm 12 is additionally provided with the periphery wall of centrifugation outer tube 10.
To realize effective closure to the push pipe mouth of impermeable inner tube 20, so as to ensure the sealing property of whole centrifuge tube, this The sealing gag 30 of embodiment includes one and is socketed simultaneously with centrifuging the upper end of outer tube 10 and the upper end screw thread of impermeable inner tube 20 Cannula portion 31, a clamped through hole (not marked in figure) is offered on the top wall surface of cannula portion 31, is installed in clamped through hole Have a rubber seal plug 32, the end face center region of rubber seal plug 32 in axial direction to after lower recess formed with a ball groove e, The conical plug f being placed in after bottom center region is in axial direction downwardly convex formed with one in the upper end of impermeable inner tube 20.Such as This, provides accurate positioning action, simultaneously when being punctured using ball groove e for instruments such as syringes to sealing gag 30 Because conical plug f and ball groove e setting causes whole sealing gag 30 integrally appearing similar to the structure type of " V " shape, It can be that syringe provides enough pull-up strokes during extraction, avoid sealing gag 30 (especially in puncture withdrawal process It is rubber seal plug 32) it is separated with centrifugation outer tube 10 or impermeable inner tube 20.
As a preferred scheme, to further enhance the structural integrity between rubber seal plug 32 and cannula portion 31, An annular groove (not marked in figure) is offered on the perisporium of rubber seal plug 31, correspondingly, is formed on the hole wall of clamped through hole There are the ring convex 33 that a contraposition is embedded in annular groove.
Preferred embodiment of the present utility model is the foregoing is only, not thereby limits the scope of the claims of the present utility model, Every equivalent structure made using the utility model specification and accompanying drawing content or equivalent flow conversion, or directly or indirectly fortune Used in other related technical areas, similarly it is included in scope of patent protection of the present utility model.

Claims (7)

  1. A kind of 1. infiltration type centrifuge tube, it is characterised in that:It includes top end opening and the centrifugation outer tube of bottom end closure, outer by centrifuging The top ports sleeve of pipe is in the impermeable inner tube in the upper end of centrifugation outer tube and will centrifuge the upper end of outer tube and impermeable inner tube Socket sealing gag;The impermeable inner tube is the tubular body structure that top and bottom offer the mouth of pipe, the impermeable inner tube The permeable screens for being used to the push pipe mouth of impermeable inner tube and bottom tube mouth being separated, the permeable screens are equiped with upper end For the trapezoidal bag shaped structure body of axial cross section, the port side wall of the permeable screens and the internal perisporium of impermeable inner tube are fixed as one Body.
  2. A kind of 2. infiltration type centrifuge tube as claimed in claim 1, it is characterised in that:The permeable screens are by composite fibre micropore Filter membrane or polyethersulfone membranes enclose shaping.
  3. A kind of 3. infiltration type centrifuge tube as claimed in claim 1, it is characterised in that:The impermeable inner tube includes pressing on centrifugation Fixation ring portion on the end face of the top ports of outer tube and the bottom surface by fixation ring portion and the circular inner ring opening's edge axle for fixing ring portion The interior pipe portion of aftershaping is extended downwardly to direction, is provided with the periphery wall of the fixed ring portion for connecting with sealing gag screw thread The the first external screw thread area connect, be provided with the periphery wall of the upper end in said inner tube portion for be connected with centrifugation external pipe thread the Two external screw thread areas, the trapezoidal relief port of the axial cross section that is opened up on the bottom in said inner tube portion is as impermeable inner tube Bottom tube mouth, the port side wall of the permeable screens are fixed on the collar extension perisporium of fixed ring portion, the upper end of the centrifugation outer tube Periphery wall on be provided with for the 3rd external screw thread area that is threadedly coupled of sealing gag.
  4. A kind of 4. infiltration type centrifuge tube as claimed in claim 3, it is characterised in that:The inner circumferential of the upper end of the centrifugation outer tube Be provided with wall one be used for block be formed at interior pipe portion periphery wall and centrifugation outer tube internal perisporium between gap closure ring.
  5. A kind of 5. infiltration type centrifuge tube as claimed in claim 3, it is characterised in that:Also set on the periphery wall of the centrifugation outer tube It is equipped with support arm.
  6. A kind of 6. infiltration type centrifuge tube as any one of claim 1-5, it is characterised in that:The sealing gag includes One cannula portion being socketed simultaneously with the upper end of centrifugation outer tube and the upper end screw thread of impermeable inner tube, the top wall surface in described sleeve pipe portion On offer a clamped through hole, a rubber seal plug, the end face center of the rubber seal plug are equiped with the clamped through hole Region in axial direction to after lower recess formed with a ball groove, bottom center region it is in axial direction downwardly convex after formed with one The conical plug being placed in the upper end of impermeable inner tube.
  7. A kind of 7. infiltration type centrifuge tube as claimed in claim 6, it is characterised in that:Opened up on the perisporium of the rubber seal plug There are an annular groove, the ring convex being embedded on the hole wall of the clamped through hole formed with a contraposition in annular groove.
CN201720471143.XU 2017-04-27 2017-04-27 A kind of infiltration type centrifuge tube Active CN206746829U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720471143.XU CN206746829U (en) 2017-04-27 2017-04-27 A kind of infiltration type centrifuge tube

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720471143.XU CN206746829U (en) 2017-04-27 2017-04-27 A kind of infiltration type centrifuge tube

Publications (1)

Publication Number Publication Date
CN206746829U true CN206746829U (en) 2017-12-15

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Country Status (1)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109932212A (en) * 2019-04-18 2019-06-25 首都医科大学附属北京安贞医院 It is centrifuged extraction element
CN117192048A (en) * 2023-09-06 2023-12-08 武汉轻工大学 Test tube device convenient for removing milk fat and rapidly measuring milk fat content

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109932212A (en) * 2019-04-18 2019-06-25 首都医科大学附属北京安贞医院 It is centrifuged extraction element
CN117192048A (en) * 2023-09-06 2023-12-08 武汉轻工大学 Test tube device convenient for removing milk fat and rapidly measuring milk fat content

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GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20220121

Address after: 518000 west side of floor 7, building a, No. 2, Luozu Industrial Avenue, Luozu community, Shiyan street, Bao'an District, Shenzhen, Guangdong Province

Patentee after: Shenzhen Kekang Clinical Medicine Research Co.,Ltd.

Address before: 518000 Room 201, building A, No. 1, Qian Wan Road, Qianhai Shenzhen Hong Kong cooperation zone, Shenzhen, Guangdong (Shenzhen Qianhai business secretary Co., Ltd.)

Patentee before: SHENZHEN QIANHAI PRECISION BIO-TECH CO.,LTD.

TR01 Transfer of patent right