CN209790967U

CN209790967U - Device for extracting mononuclear cells in blood

Info

Publication number: CN209790967U
Application number: CN201920356817.0U
Authority: CN
Inventors: 林侯汎
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-03-20
Filing date: 2019-03-20
Publication date: 2019-12-17
Anticipated expiration: 2029-03-20

Abstract

The embodiment of the utility model discloses a device for extracting mononuclear cells in blood, which comprises a tube body, a piston and a push rod, wherein the push rod is in threaded connection with the tube body, and the piston is fixedly connected with the push rod; the pipe body is divided into an upper pipe area, an extraction area and a lower pipe area, and the extraction area is positioned between the upper pipe area and the lower pipe area; the piston is positioned on the inner side of the lower pipe area and is in sealed sliding connection with the inner wall of the lower pipe area; one end of the push rod, which is far away from the piston, is positioned outside the lower pipe area, and the push rod drives the piston to move along the axial direction of the lower pipe area when rotating relative to the pipe body; one end of the upper pipe area, which is far away from the lower pipe area, is provided with a first jack, and the inner space of the upper pipe area is communicated with the outer space through the first jack; the extraction area is provided with an air hole, and the internal space of the extraction area is communicated with the external space through the air hole; its advantages are high sucking efficiency, isolating external air, no need of external solution, and no pollution to blood.

Description

device for extracting mononuclear cells in blood

Technical Field

the embodiment of the utility model provides a relate to medical equipment technical field, concretely relates to a device for extracting mononuclear cell in blood.

Background

In some medical detection and experiment processes, blood mononuclear cells are taken as materials, blood, tissue fluid and the like are usually separated firstly, plasma rich in mononuclear cells is obtained by centrifuging fresh blood (umbilical cord blood) in a centrifugal mode, the plasma is rich in various growth factors and serves as nutrients of autologous cells, mononuclear cells in peripheral blood or umbilical cord blood are developed to treat tumor immunity clinical adjuvant therapy at present, and the mononuclear cells in the umbilical cord blood can also serve as a source of a large amount of blood/red blood cells later. It is prepared by separating, freezing, culturing, and returning to original body after adding therapeutic properties.

However, the current separation method generally adopts a needle cylinder or a blood collection tube to collect blood, and then sends the blood to a sterile laboratory for centrifugal acquisition, the blood is separated or mixed with other additional solutions, the centrifugation is carried out, an operation device is not closed, the pollution is easy to occur in the process, the safety of the additional solutions and the environmental requirements are strict, the blood collection centrifugation operation must be completed in different devices, and the quality of the plasma rich in mononuclear cells is difficult to ensure by integrating the processes.

SUMMERY OF THE UTILITY MODEL

Therefore, the embodiment of the utility model provides a device for drawing mononuclear cell in blood, the in-process need not to mix with other plus solutions, reduces the chance with outside air or solution contact, and the pollution abatement to improve efficiency and the security of isolating mononuclear cell from blood.

In order to achieve the above object, the embodiment of the present invention provides the following technical solutions:

A device for extracting mononuclear cells in blood comprises a tube body, a piston and a push rod, wherein the push rod is in threaded connection with the tube body, and the piston is fixedly connected with the push rod;

The pipe body is divided into an upper pipe area, an extraction area and a lower pipe area, and the extraction area is positioned between the upper pipe area and the lower pipe area;

The piston is positioned on the inner side of the lower pipe area and is in sealed sliding connection with the inner wall of the lower pipe area;

One end of the push rod, which is far away from the piston, is positioned outside the lower pipe area, and the push rod drives the piston to move along the axial direction of the lower pipe area when rotating relative to the pipe body;

one end of the upper pipe area, which is far away from the lower pipe area, is provided with a first jack, and the inner space of the upper pipe area is communicated with the outer space through the first jack;

The extraction area is provided with an air hole, and the inner space of the extraction area is communicated with the outer space through the air hole.

The embodiment of the utility model provides a further setting up to: the one end in the area of being in the area of keeping away from in the upper tube is rotated and is connected with the upper cover, covers on and has seted up the second jack, first jack and second jack are relative deviating from the upper cover respectively the rotatory the central axis of body, the relative body rotation back of upper cover, the axial alignment in the area of being in the upper tube or staggering can be followed to first jack and second jack.

The embodiment of the utility model provides a further setting up to: the upper cover is in threaded connection with the pipe body.

The embodiment of the utility model provides a further setting up to: and one end of the lower pipe area, which is far away from the upper pipe area, is fixedly covered with a bottom cover, and the push rod is in threaded connection with the bottom cover.

the embodiment of the utility model provides a further setting up to: the inner diameter of the extraction zone is smaller than the diameter of the upper and lower tube zones.

the embodiment of the utility model provides a further setting up to: the volume of the upper tube area is equal to the volume of the lower tube area.

The embodiment of the utility model provides a further setting up to: the tube body is transparent.

the embodiment of the utility model provides a further setting up to: the air hole is positioned at one end of the extraction area close to the lower pipe area;

the embodiment of the utility model provides a further setting up to: and a rubber plug is connected to one end of the upper pipe area, which is far away from the lower pipe area, of the sealing plug.

The embodiment of the utility model provides a further setting up to: the rubber stopper is fixedly connected with the first jack in a sealing mode.

The embodiment of the utility model provides an advantage is: during operation, open the gas pocket card and insert the body and with the blood injection body from first jack with the syringe needle that will be equipped with blood, treat that blood is full of the lower tube district after, stop up the gas pocket, continue to pour into the body with blood into the body until blood is full of extraction district and top tube district, extract the syringe needle of syringe from the body in, rotatory upper cover makes first jack and second jack stagger each other, blocks the intercommunication of body inner space and exterior space. Loading the tube body into a centrifuge for centrifugation, wherein the distance between an upper tube area and a rotation central shaft during centrifugal motion is smaller than the distance between a lower tube area and the rotation central shaft, the density of plasma is smaller than that of blood cells, the density of mononuclear cells is between the plasma and red blood cells, the blood is divided into three layers after centrifugation, the plasma is positioned in the upper tube area, the red blood cells are positioned in the lower tube area, the mononuclear cells are positioned between the plasma and the red blood cells, and a part of the mononuclear cells are also positioned in the lower tube area. Taking the tube out of the centrifuge, rotating the push rod in the precession direction, pushing the mononuclear cells by the piston to gradually converge to the extraction area, sequentially penetrating the needle head of the injector into the extraction area through the first jack and the second jack, and sucking the mononuclear cells into the injector to complete collection. During the separation and collection process, the exposure of blood to air can be reduced, and the pollution of mononuclear cells to the air can be reduced. In the process of extracting the mononuclear cells by using the injector, the needle head extends to the bottom of the extraction area, negative pressure is generated inside the extraction area in the extraction process, and the plasma gradually moves to the extraction area in the direction of entering the extraction area under the action of self gravity and the negative pressure of the extraction area, so that the mononuclear cells are collected more thoroughly, and the extraction efficiency is improved.

drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.

The structure, ratio, size and the like shown in the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by people familiar with the technology, and are not used for limiting the limit conditions which can be implemented by the present invention, so that the present invention has no technical essential significance, and any structure modification, ratio relationship change or size adjustment should still fall within the scope which can be covered by the technical content disclosed by the present invention without affecting the efficacy and the achievable purpose of the present invention.

Fig. 1 is a cross-sectional view of embodiment 1 of the present invention, cut along the central axis of the pipe body;

Fig. 2 is a schematic view showing a connection structure between a rubber stopper and a pipe body in embodiment 2 of the present invention;

fig. 3 is a schematic structural diagram of embodiment 3 of the present invention.

in the figure:

1. A pipe body; 11. an upper tube zone; 12. an extraction zone; 13. a lower tube zone; 14. a first jack; 15. air holes; 2. a piston; 3. a push rod; 4. an upper cover; 41. a second jack; 5. a bottom cover; 6. and a rubber plug.

Detailed Description

the present invention is described in terms of specific embodiments, and other advantages and benefits of the present invention will become apparent to those skilled in the art from the following disclosure. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example 1

A device for extracting mononuclear cells in blood is shown in figure 1 and comprises a tube body 1, a piston 2 and a push rod 3, wherein the push rod 3 is in threaded connection with the tube body 1, and the piston 2 is fixedly connected with the push rod 3; the pipe body 1 is divided into an upper pipe area 11, an extraction area 12 and a lower pipe area 13, and the extraction area 12 is positioned between the upper pipe area 11 and the lower pipe area 13; the piston 2 is positioned on the inner side of the lower pipe area 13, and the piston 2 is in sealed sliding connection with the inner wall of the lower pipe area 13; one end of the push rod 3, which is far away from the piston 2, is positioned outside the lower pipe area 13, and the push rod 3 drives the piston 2 to move along the axial direction of the lower pipe area 13 when rotating relative to the pipe body 1; one end of the upper tube area 11, which is far away from the lower tube area 13, is provided with a first jack 14, and the inner space of the upper tube area 11 is communicated with the outer space through the first jack 14; the extraction area 12 is provided with an air hole 15, and the inner space of the extraction area 12 is communicated with the outer space through the air hole 15.

When the blood injection syringe works, the air hole 15 is clamped (the air hole 15 is not needed to be plugged by a plug) to insert the syringe needle filled with blood into the tube body 1 from the first insertion hole 14, the blood is injected into the tube body 1, after the lower tube area 13 is filled with the blood, the air hole 15 is plugged, the blood is continuously injected into the tube body 1 until the blood is filled into the extraction area 12 and the upper tube area 11, the needle of the syringe is pulled out from the tube body 1, the upper cover 4 is rotated, the first insertion hole 14 and the second insertion hole 41 are staggered, and the communication between the inner space and the outer space of the tube body 1 is blocked. The tube body 1 is loaded into a centrifuge for centrifugation, the distance between an upper tube area 11 and a rotation central shaft is smaller than the distance between a lower tube area 13 and the rotation central shaft during centrifugation, the density of plasma is smaller than that of blood cells, the density of mononuclear cells is between the plasma and the red blood cells, the blood is divided into three layers after centrifugation, the plasma is positioned in the upper tube area 11, the red blood cells are positioned in the lower tube area 13, the mononuclear cells are positioned between the plasma and the red blood cells, and a part of the mononuclear cells are also positioned in the lower tube area 13. Taking the tube body 1 out of the centrifuge, rotating the push rod 3 towards the screwing direction, pushing the mononuclear cells to gradually converge to the extraction area 12 by the piston, sequentially passing the needle of the injector through the first jack 14 and the second jack 41 to extend into the extraction area, and absorbing the mononuclear cells into the injector to finish collection. During the separation and collection process, the exposure of blood to air can be reduced, and the pollution of mononuclear cells to the air can be reduced. In the process of extracting the mononuclear cells by using the injector, the needle head extends to the bottom of the extraction area 12, negative pressure is generated inside the extraction area 2 in the extraction process, and the plasma gradually moves to the extraction area in the direction of entering the extraction area 2 under the action of the self gravity and the negative pressure of the extraction area 2, so that the mononuclear cells are collected more thoroughly, and the extraction efficiency is improved.

The one end rotation that the area 13 was controlled in the lower tube in 11 was kept away from in the upper tube in area is connected with upper cover 4, has seted up second jack 41 on the upper cover 4, and first jack 14 and second jack 41 deviate the central axis of the relative body 1 rotation of upper cover 4 respectively, and the relative body 1 rotation back of upper cover 4, first jack 14 and second jack 41 can follow the axial alignment or stagger of last pipe area 11.

The upper cover 4 is in threaded connection with the pipe body 1. One end of the lower tube area 13 far away from the upper tube area 11 is fixedly covered with a bottom cover 5, and the push rod 3 is in threaded connection with the bottom cover 5.

The inner diameter of the extraction zone 12 is smaller than the diameter of the upper and lower tube zones 11, 13. The upper tube section 11 has the same volume as the lower tube section 13. The tube 1 is transparent. The air hole 15 is located at an end of the extraction region 12 near the lower tube region 13.

Example 2

The utility model provides a device for extracting interior mononuclear cell of blood, as shown in figure 2, the difference with embodiment 1 lies in, upper cover 4 is rubber buffer 6, rubber buffer 6 sealing plug connects 11 one end of keeping away from 13 in the area of managing under the last area of managing, in operation, insert the syringe needle of syringe and pass rubber buffer 6 in order to stretch into body 1, the syringe needle inserts body 1 or extracts the in-process from body 1, rubber buffer 6 can avoid inside the outside air admission body, avoid the blood in the body 1 to receive the pollution.

example 3

A device for extracting mononuclear cells in blood, as shown in FIG. 3, is different from the device of example 1 in that a rubber stopper 6 is fixedly and hermetically connected in a first insertion hole 14.

Although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Therefore, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. An apparatus for extracting mononuclear cells from blood, comprising: the piston type air-breathing device comprises a pipe body (1), a piston (2) and a push rod (3), wherein the push rod (3) is in threaded connection with the pipe body (1), and the piston (2) is fixedly connected with the push rod (3);

the pipe body (1) is divided into an upper pipe area (11), an extraction area (12) and a lower pipe area (13), and the extraction area (12) is positioned between the upper pipe area (11) and the lower pipe area (13);

The piston (2) is positioned on the inner side of the lower pipe area (13), and the piston (2) is in sealed sliding connection with the inner wall of the lower pipe area (13);

One end of the push rod (3) far away from the piston (2) is positioned outside the lower pipe area (13), and the push rod (3) drives the piston (2) to move along the axial direction of the lower pipe area (13) when rotating relative to the pipe body (1);

one end of the upper pipe area (11) far away from the lower pipe area (13) is provided with a first jack (14), and the inner space of the upper pipe area (11) is communicated with the outer space through the first jack (14);

The extraction area (12) is provided with an air hole (15), and the inner space of the extraction area (12) is communicated with the outer space through the air hole (15).

2. The apparatus according to claim 1, wherein: the one end rotation that upper tube area (11) was kept away from lower tube area (13) is connected with upper cover (4), has seted up second jack (41) on upper cover (4), first jack (14) and second jack (41) are relative deviating from upper cover (4) respectively the rotatory central axis of body (1), upper cover (4) relative body (1) rotatory back, the axial alignment or the stagger in upper tube area (11) can be followed in first jack (14) and second jack (41).

3. A device for extracting mononuclear cells from blood as claimed in claim 2, characterized in that: the upper cover (4) is in threaded connection with the pipe body (1).

4. The apparatus according to claim 1, wherein: one end, far away from the upper tube area (11), of the lower tube area (13) is fixedly covered with a bottom cover (5), and the push rod (3) is in threaded connection with the bottom cover (5).

5. The apparatus according to claim 1, wherein: the inner diameter of the extraction zone (12) is smaller than the diameter of the upper (11) and lower (13) zones.

6. The apparatus according to claim 1, wherein: the volume of the upper tube area (11) is equal to the volume of the lower tube area (13).

7. The apparatus according to claim 1, wherein: the tube body (1) is transparent.

8. The apparatus according to claim 1, wherein: the air hole (15) is positioned at one end of the extraction area (12) close to the lower pipe area (13).

9. The apparatus according to claim 1, wherein: one end of the upper pipe area (11) far away from the lower pipe area (13) is sealed and connected with a rubber plug (6).

10. A device for extracting mononuclear cells from blood as claimed in claim 2, characterized in that: a rubber plug (6) is fixedly connected in the first insertion hole (14) in a sealing manner.