CN210261612U

CN210261612U - Ultrafiltration tube for RXR- α phosphorylated antibody

Info

Publication number: CN210261612U
Application number: CN201920574922.1U
Authority: CN
Inventors: 林世康
Original assignee: Nanjing Chuanbo Biotechnology Co ltd
Current assignee: Nanjing Chuanbo Biotechnology Co ltd
Priority date: 2019-04-25
Filing date: 2019-04-25
Publication date: 2020-04-07
Anticipated expiration: 2029-04-25

Abstract

The utility model relates to a biological research technical field just discloses a RXR- α ultra-filtration tube for phosphorylation antibody, including the centrifuging tube, the filter tube has been cup jointed to the intraductal activity of centrifuging tube, the lower extreme fixedly connected with conical duct of filter tube, two mounting grooves have been seted up to the outer wall symmetry of conical duct, fixedly connected with filtration membrane in the mounting groove, the screw thread cover is equipped with first closing cap on the centrifuging tube, the filter tube is close to the one end fixedly connected with collar plate of first closing cap, the collar plate is located between first closing cap and the centrifuging tube, the external diameter of collar plate is greater than the internal diameter of centrifuging tube.

Description

Ultrafiltration tube for RXR- α phosphorylated antibody

Technical Field

The utility model relates to a biological research technical field especially relates to an RXR- α phosphorylation antibody is with ultrafiltration pipe.

Background

The main function of the phosphorylated RXR- α antibody is to bind to the antigen (including foreign and self) to effectively remove foreign substances such as microbes and parasites that invade the body, and the research and test on the phosphorylated RXR- α antibody requires high speed centrifugation using an ultrafiltration tube having a rapid ultrafiltration function, which can achieve a high concentration factor, and can easily recover a concentrated solution from a diluted solution or a complex sample combination, and the vertical design and the available surface area of the filter membrane can provide a rapid sample processing speed and a high sample recovery rate, and can perform 80-fold concentration.

Present ultrafiltration tube is after high-speed centrifugation is accomplished, the concentrate is finally preserved in the bottom of ultrafiltration tube, because the degree of depth of ultrafiltration tube is great, it is inconvenient to lead to the concentrate to take out, need open the lid and take out the filter, then use dedicated pipette to come to take out the concentrate, it is comparatively loaded down with trivial details and time to operate, lead to the concentrate can't take out immediately, can not reach the ideal and retrieve, influence the concentrate and detect the accuracy, and the filter of present ultrafiltration tube lacks stable stop gear, rock easily at high-speed centrifugation, lead to the life reduction of filter.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the problem that the filter of getting liquid operation process among the prior art is loaded down with trivial details and ultrafiltration pipe rocks easily when centrifuging, and the ultrafiltration pipe for RXR- α phosphorylation antibody that provides.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a RXR- α ultra-filtration tube for phosphorylation antibody, includes the centrifuging tube, the filtering tube has been cup jointed to the intraductal activity of centrifuging tube, the lower extreme fixedly connected with conical tube of filtering tube, two mounting grooves have been seted up to the outer wall symmetry of conical tube, fixedly connected with filtration membrane in the mounting groove, the screw thread cover is equipped with first closing cap on the centrifuging tube, the filtering tube is close to the one end fixedly connected with collar plate of first closing cap, the collar plate is located between first closing cap and the centrifuging tube, the external diameter of collar plate is greater than the internal diameter of centrifuging tube, the through-hole has been seted up to the lower extreme of centrifuging tube, and the pore wall fixedly connected with drain pipe of through-hole, the through-hole is stretched out to the lower extreme of drain pipe, the lower extreme screw thread cover of drain pipe is equipped with the second closing cap, the first sealed piece of inner wall fixedly connected.

Preferably, two gag lever posts of the lateral wall symmetry fixedly connected with of centrifuging tube upside, the lateral wall symmetry that the collar plate is close to centrifuging tube one side is seted up two spacing grooves corresponding with the gag lever post, the one end that the centrifuging tube was kept away from to the gag lever post is located the spacing inslot, a plurality of stoppers of inner wall fixedly connected with of centrifuging tube.

Preferably, the lateral wall fixedly connected with that first closing cap is close to centrifuging tube one side is sealed to be filled up, sealed pad and the apron in close contact with, sealed pad is close to the sealed piece of lateral wall fixedly connected with second of filter tube one side, the sealed piece of second and the inner wall in close contact with of filter tube.

Preferably, the lateral wall fixedly connected with dead lever of centrifuging tube one side is kept away from to first closing cap, the dead lever is kept away from the first spacing ball of one end fixedly connected with of first closing cap, the pole wall movable sleeve of dead lever is equipped with changes the board, the rotation hole has been seted up to the lateral wall of changeing the board, the dead lever runs through the rotation hole and changes the board, the lateral wall fixedly connected with connecting band of changeing the board, the connecting band is kept away from the one end of changeing the board and the outer wall fixed connection of centrifuging tube.

Preferably, the taper of the conical pipe is 30-50 degrees.

Preferably, the outer wall of centrifuging tube is carved with anti-skidding line, anti-skidding line is close to the upper end of centrifuging tube.

Preferably, the lateral wall fixedly connected with bull stick of drain pipe one side is kept away from to the second closing cap, the spacing ball of one end fixedly connected with second of second closing cap is kept away from to the bull stick, the pole wall movable sleeve of bull stick is equipped with the connecting plate, the trepanning has been seted up to the lateral wall of connecting plate, the bull stick passes through the trepanning and runs through the connecting plate, the lateral wall fixedly connected with fixed band of connecting plate, the fixed band is kept away from the one end of connecting plate and the outer wall fixed connection of centrifuging tube.

Preferably, the specific material of the first limiting ball is stainless steel.

Preferably, the specific materials of the first sealing block and the limiting block are rubber.

Compared with the prior art, the utility model provides a RXR- α phosphorylation antibody is with ultrafiltration pipe possesses following beneficial effect:

1. this RXR- α ultra-filtration tube for phosphorylation antibody, through the centrifuging tube that sets up, the filter tube, the conical tube, the mounting groove, filtration membrane, first closing cap, the apron, the drain pipe, the first sealing block of second closing cap HE, in use, the sample in the filter tube filters through filtration membrane after centrifuge is quick centrifugation, the sample concentrate after the filtration gets into the lower extreme of centrifuging tube, when needing to take out the concentrate, rotate the second closing cap, make the second closing cap break away from the drain pipe, relieve the second closing cap and to the sealing of drain pipe, pour the concentrate from the mouth of pipe of drain pipe, accomplish the operation of taking out of concentrate, and easy operation is convenient, operation steps are simplified, the time that takes out the concentrate has been reduced, the concentrate can be more idealized recovery, the accuracy nature of testing result has been effectively improved.

2. This RXR- α phosphorylation antibody uses ultra filter tube, through the gag lever post that sets up, spacing groove and stopper, during the use, insert the filter tube in the centrifuging tube, rotate the filter tube, the filter tube drives the crown plate and rotates, make the gag lever post of spacing groove alignment, promote the crown plate, make the gag lever post insert the spacing groove, mutually support through gag lever post and spacing groove, can carry out effectual spacing fixed to the crown plate, thereby fix the filter tube with crown plate fixed connection, the stopper of a plurality of rubber materials of setting can carry out effectual spacing to the filter tube, prevent that the filter tube from rocking when rotating at a high speed, this device can carry out spacing fixed to the filter tube, the effectual problem of rocking easily of filter tube at high-speed centrifugation in-process of having avoided, the effectual life who improves the filter tube.

The device does not relate to the part and all is the same with prior art or can adopt prior art to realize, the utility model discloses simplified operating procedure, reduced the required time of taking out the concentrate, the recovery concentrate that can more idealization, the effectual accuracy nature that improves the testing result can carry out spacing fixed to the filter tube, and the effectual problem of having avoided the filter tube to rock easily at high-speed centrifugal in-process has effectually improved the life of filter tube.

Drawings

Fig. 1 is a schematic structural diagram of an ultrafiltration tube for RXR- α phosphorylated antibody according to the present invention;

FIG. 2 is a schematic structural view of portion A of FIG. 1;

FIG. 3 is a schematic structural diagram of portion B of FIG. 2;

FIG. 4 is a schematic structural view of portion C of FIG. 1;

fig. 5 is a schematic structural view of a portion D in fig. 1.

In the figure: 1 centrifuging tube, 2 filter tubes, 3 taper tubes, 4 mounting grooves, 5 filtration membranes, 6 first closing caps, 7 rings of boards, 8 drain pipes, 9 second closing caps, 10 first sealing blocks, 11 limiting rods, 12 limiting grooves, 13 limiting blocks, 14 sealing gaskets, 15 second sealing blocks, 16 fixing rods, 17 first limiting balls, 18 rotating plates, 19 rotating holes, 20 connecting belts, 21 anti-slip patterns, 22 rotating rods, 23 second limiting balls, 24 connecting plates, 25 trepanning holes and 26 fixing belts.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Referring to fig. 1-5, an RXR- α ultra-filtration tube for phosphorylation antibody, including centrifuging tube 1, filter tube 2 has been cup jointed to the activity in centrifuging tube 1, filter tube 2's lower extreme fixedly connected with conical tube 3, two mounting grooves 4 have been seted up to the outer wall symmetry of conical tube 3, fixedly connected with filtration membrane 5 in mounting groove 4, centrifuging tube 1 upper end thread bush is equipped with first closing cap 6, filter tube 2 is close to one end fixedly connected with collar plate 7 of first closing cap 6, collar plate 7 is located between first closing cap 6 and centrifuging tube 1, the external diameter of collar plate 7 is greater than centrifuging tube 1's internal diameter, the through-hole has been seted up to centrifuging tube 1's lower extreme, and the pore wall fixedly connected with drain pipe 8 of through-hole, the through-hole stretches out to the lower extreme of drain pipe 8, the lower extreme thread bush of drain pipe 8 is equipped with second closing cap 9, the first seal block 10 of inner wall fixedly connected with drain pipe 8, first seal block 10 and the inner wall in close contact with drain pipe 8, in use, after the sample in the filter tube 2 filters through-filtering membrane 5 after-centrifugation fast, the concentrated concentrate liquid that the sample liquid after-filtering, need rotate, when the concentrated liquid that the operation of second closing cap is taken out, the concentrate can be taken out, the concentrate operation of the concentrate can be taken out, the concentrate is taken out, the concentrate can be taken out of centrifuge tube 8, the concentrate, the.

The side wall of the upper side of the centrifugal tube 1 is symmetrically and fixedly connected with two limiting rods 11, the side wall of the ring plate 7 close to one side of the centrifugal tube 1 is symmetrically provided with two limiting grooves 12 corresponding to the limiting rods 11, one end of the limiting rod 11 far away from the centrifugal tube 1 is positioned in the limiting grooves 12, the inner wall of the centrifugal tube 1 is fixedly connected with a plurality of limiting blocks 13, when in use, the filter tube 2 is inserted into the centrifugal tube 1, the filter tube 2 is rotated, the filter tube 2 drives the ring plate 7 to rotate, the limiting grooves 12 are aligned with the limiting rods 11, the ring plate 7 is pushed, the limiting rods 11 are inserted into the limiting grooves 12, the ring plate 7 can be effectively limited and fixed through the mutual matching of the limiting rods 11 and the limiting grooves 12, so that the filter tube 2 fixedly connected with the ring plate 7 is fixed, the limiting blocks 13 made of a plurality of rubber materials can effectively limit the filter tube 2, and the filter, this device can carry out spacing fixed to filter tube 2, the effectual problem of having avoided filter tube 2 to rock easily at high-speed centrifugal in-process, the effectual life who improves filter tube 2.

First closing cap 6 is close to sealed 14 of lateral wall fixedly connected with of centrifuge tube 1 one side, sealed 14 and the 7 in close contact with of circle board, sealed 14 lateral wall fixedly connected with second of being close to 2 one sides of filter tube seals up piece 15, the inner wall in close contact with of second sealed piece 15 and filter tube 2, sealed 14 that sets up can seal up the junction of circle board 7 and first closing cap 6, sealed piece 15 of second can seal up the upper end of filter tube 2, sealed piece 15 of second has better elastic property, when sealed piece 15 of second inserts filter tube 2, can be with the inner wall in close contact with of filter tube 2 under the effect of self resilience power, can effectually prevent that the sample from leaking from the upper end of filter tube 2 when high-speed centrifugation.

Lateral wall fixedly connected with dead lever 16 that first closing cap 6 kept away from centrifuging tube 1 one side, dead lever 16 keeps away from the first spacing ball 17 of one end fixedly connected with of first closing cap 6, the pole wall movable sleeve of dead lever 16 is equipped with changes board 18, change the lateral wall of board 18 and seted up rotation hole 19, dead lever 16 runs through commentaries on classics board 18 through rotating hole 19, change the lateral wall fixedly connected with connecting band 20 of board 18, connecting band 20 keeps away from the one end of changeing board 18 and the outer wall fixed connection of centrifuging tube 1, during the use, rotate first closing cap 6, first closing cap 6 drives dead lever 16 and rotates, because change board 18 movable sleeve and establish outside dead lever 16, so can not influence the normal rotation of first closing cap 6 with the connecting band 20 of changeing board 18 fixed connection, when first closing cap 6 breaks away from centrifuging tube 1, connecting band 20 can prevent that first closing cap 6 from dropping and losing.

The taper of the conical tube 3 is 30-50 degrees, so that a sample can be conveniently contacted with the filtering membrane 5, and the filtering effect is improved.

The outer wall of centrifuging tube 1 is carved with anti-skidding line 21, and anti-skidding line 21 is close to the upper end of centrifuging tube 1, and anti-skidding line 21 can avoid the problem that the hand slided when taking centrifuging tube 1 leads to centrifuging tube 1 to damage.

Lateral wall fixedly connected with bull stick 22 that second closing cap 9 kept away from drain pipe 8 one side, bull stick 22 keeps away from the spacing ball 23 of one end fixedly connected with second of second closing cap 9, the pole wall movable sleeve of bull stick 22 is equipped with connecting plate 24, trepanning 25 has been seted up to the lateral wall of connecting plate 24, bull stick 22 runs through connecting plate 24 through trepanning 25, the lateral wall fixedly connected with fixed band 26 of connecting plate 24, the one end that connecting plate 24 was kept away from to fixed band 26 and centrifuge tube 1's outer wall fixed connection, during the use, rotate second closing cap 9, second closing cap 9 drives bull stick 22 and rotates, because the connecting plate 24 movable sleeve is established outside bull stick 22, so fixed band 26 with connecting plate 24 fixed connection can not influence the normal rotation of second closing cap 9, when second closing cap 9 breaks away from drain pipe 8, fixed band 26 can prevent that second closing cap 9 from dropping and losing.

The specific material of first spacing ball 17 is stainless steel, improves first spacing ball 17's corrosion resisting property, avoids first spacing ball 17 to rust, has improved first spacing ball 17's life.

The first sealing block 10 and the limiting block 13 are made of rubber, the first sealing block 10 is made of rubber, the rubber has good elasticity, when the first sealing block 10 is inserted into the filter tube, the first sealing block can be tightly attached to the inner wall of the liquid outlet tube, the liquid outlet tube can be effectively sealed, and concentrated liquid is prevented from leaking from the joint of the second sealing cover 9 and the liquid outlet tube 8; the limiting block 13 made of rubber is soft and cannot scrape the filter pipe 2.

In the utility model, when in use, the sample in the filter tube 2 is filtered by the filter membrane 5 after being rapidly centrifuged by the centrifuge, the filtered sample concentrated solution enters the lower end of the centrifuge tube 1, when the concentrated solution needs to be taken out, the second sealing cover 9 is rotated to separate the second sealing cover 9 from the liquid outlet tube 8, the sealing of the second sealing cover 9 to the liquid outlet tube 8 is removed, the concentrated solution is poured out from the tube orifice of the liquid outlet tube 8, the concentrated solution taking-out operation is completed, the operation is simple and convenient, the operation steps are simplified, the time for taking out the concentrated solution is reduced, the concentrated solution can be more ideally recovered, and the accuracy of the detection result is effectively improved; during the use, insert filter tube 2 in centrifuging tube 1, rotate filter tube 2, filter tube 2 drives collar plate 7 and rotates, make spacing groove 12 aim at gag lever post 11, promote collar plate 7, make gag lever post 11 insert spacing groove 12, mutually support through gag lever post 11 and gag lever post 12, can carry out effectual spacing fixed to collar plate 7, thereby fix filter tube 2 with collar plate 7 fixed connection, the stopper 13 of a plurality of rubber materials of setting can carry out effectual spacing to filter tube 2, prevent that filter tube 2 from rocking when rotating at a high speed, this device can carry out spacing fixed to filter tube 2, the effectual problem of filter tube 2 rocking easily at high-speed centrifugal in-process of having avoided, the effectual life who improves filter tube 2.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The utility model provides a RXR- α ultra-filtration tube for phosphorylation antibody, includes centrifuging tube (1), its characterized in that, centrifuging tube (1) internalization has cup jointed filter tube (2), lower extreme fixedly connected with conical tube (3) of filter tube (2), two mounting groove (4) have been seted up to the outer wall symmetry of conical tube (3), fixedly connected with filtration membrane (5) in mounting groove (4), the threaded sleeve is equipped with first closing cap (6) on centrifuging tube (1), one end fixedly connected with collar plate (7) that filter tube (2) are close to first closing cap (6), collar plate (7) are located between first closing cap (6) and centrifuging tube (1), the external diameter of collar plate (7) is greater than the internal diameter of centrifuging tube (1), the through-hole has been seted up to the lower extreme of centrifuging tube (1), and the pore wall fixedly connected with drain pipe (8) of through-hole, the lower extreme of drain pipe (8) stretches out the through-hole, the lower extreme threaded sleeve of drain pipe (8) is equipped with second closing cap (9), the inner wall fixedly connected with first sealing block (10), inner wall and first sealing block (10) closely contact with drain pipe (8).

2. The ultrafiltration tube for RXR- α phosphorylated antibody according to claim 1, wherein the lateral wall of the upper side of the centrifuge tube (1) is symmetrically and fixedly connected with two limiting rods (11), the lateral wall of the collar plate (7) close to one side of the centrifuge tube (1) is symmetrically provided with two limiting grooves (12) corresponding to the limiting rods (11), one end of the limiting rod (11) far away from the centrifuge tube (1) is located in the limiting groove (12), and the inner wall of the centrifuge tube (1) is fixedly connected with a plurality of limiting blocks (13).

3. The ultrafiltration tube for RXR- α phosphorylated antibody according to claim 1, wherein the first sealing cover (6) is close to the side wall fixedly connected with sealing gasket (14) on one side of the centrifuge tube (1), the sealing gasket (14) is in close contact with the ring plate (7), the sealing gasket (14) is close to the side wall fixedly connected with second sealing block (15) on one side of the filter tube (2), and the second sealing block (15) is in close contact with the inner wall of the filter tube (2).

4. The ultrafiltration tube for RXR- α phosphorylated antibody according to claim 1, wherein the first cover (6) is kept away from the lateral wall fixedly connected with dead lever (16) on one side of the centrifuge tube (1), the dead lever (16) is kept away from the first limit ball (17) of one end fixedly connected with of the first cover (6), the movable cover of the lever wall of the dead lever (16) is provided with a rotating plate (18), the lateral wall of the rotating plate (18) is provided with a rotating hole (19), the dead lever (16) runs through the rotating plate (18) through the rotating hole (19), the lateral wall fixedly connected with connecting band (20) of the rotating plate (18), the connecting band (20) is kept away from one end of the rotating plate (18) and the outer wall fixed connection of the centrifuge tube (1).

5. The ultrafiltration tube for RXR- α phosphorylated antibody according to claim 1, wherein the taper of the tapered tube (3) is 30 ° to 50 °.

6. The ultrafiltration tube for RXR- α phosphorylated antibody according to claim 1, wherein the outer wall of the centrifuge tube (1) is engraved with anti-skid veins (21), and the anti-skid veins (21) are near the upper end of the centrifuge tube (1).

7. The ultrafiltration tube for RXR- α phosphorylated antibody according to claim 1, wherein the second cover (9) is far away from the lateral wall fixedly connected with rotating rod (22) on one side of the liquid outlet tube (8), the rotating rod (22) is far away from the one end fixedly connected with second limiting ball (23) of the second cover (9), the rod wall movable sleeve of the rotating rod (22) is provided with the connecting plate (24), the side wall of the connecting plate (24) is provided with the trepanning (25), the rotating rod (22) penetrates through the connecting plate (24) through the trepanning (25), the lateral wall fixedly connected with fixing strap (26) of the connecting plate (24), the fixing strap (26) is far away from one end of the connecting plate (24) and the outer wall fixed connection of the centrifuge tube (1).

8. The ultrafiltration tube for RXR- α phosphorylated antibody according to claim 4, wherein the specific material of the first limiting ball (17) is stainless steel.

9. The ultrafiltration tube for RXR- α phosphorylated antibody according to claim 2, wherein the specific materials of the first sealing block (10) and the limiting block (13) are both rubber.