CN207263476U - A kind of push type syringe needle for being used to draw extract - Google Patents
A kind of push type syringe needle for being used to draw extract Download PDFInfo
- Publication number
- CN207263476U CN207263476U CN201720860018.8U CN201720860018U CN207263476U CN 207263476 U CN207263476 U CN 207263476U CN 201720860018 U CN201720860018 U CN 201720860018U CN 207263476 U CN207263476 U CN 207263476U
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- China
- Prior art keywords
- chassis
- pintle
- syringe needle
- curved support
- push type
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Abstract
A kind of push type syringe needle for being used to draw extract, including pintle, pin stalk, needle point, chassis, curved support, one end connection syringe of the pintle are the utility model is related to, the other end and the pin stalk of pintle connect, and pintle and pin stalk are hollow structure；The pintle is equipped with arc recessed area, outer end of the arc recessed area towards chassis by curved support connecting base plate, chassis middle part；Pin stalk end is equipped with needle point, the bottom of the edge of needle point close to arc recessed area；The chassis is equipped with some apertures.The utility model coordinates syringe to realize in the case of without other material dissolutions, and more extracts are drawn out in pressing, and to meet the requirement of analysis, the structure of this syringe needle can effectively prevent filter membrane chip from blocking syringe needle, and easy to clean, can be used for multiple times.
Description
Technical field
The technical field for the syringe needle for obtaining membrane filtration extracting substances liquid is the utility model is related to, particularly one kind is used for
Draw the technical field of the push type syringe needle of marine phytoplankton pigment extraction liquid.
Background technology
Phytoplankton refers to the microphyte to live in water in a manner of swimming, and usual phytoplankton just refers to swim alga
Class, including diatom, dinoflagellate, hidden algae, green alga, cyanobacteria etc..Phytoplankton be distributed on earth it is very wide, be in aquatic ecosystem most
Important primary producer, plays a crucial role in energy flow in environment, material circulation and ecological community's structure.
Pigment is the chemical markers thing of phytoplankton, they are primarily involved in photosynthesis and protection pigment is taken on so that plant in part
From light injury.The measure of pigment is used not only for representing the primary productivity situation of environment, can be also used for phytoplankton
The classification of monoid.
No matter which kind of mensuration mode, be required for carry out pigment extraction, pigment extraction be measure pigment pre-treatment key
Step.Usual phytoplankton is retained on GF/F filter membranes by drainage, and when extraction needs to add organic extraction after filter membrane is shredded
Take in liquid and extracted.To ensure that pigment concentration value is higher than instrument detection limit, it is necessary to the volume of stringent control extract.Addition extraction
Take the volume of liquid according to filter membrane size by 1.5~5ml etc..To improve extraction efficiency, it usually needs carry out physical agitation and surpass
Sound crushes, and since the volume for adding extract is less, after physical agitation and ultrasound, extract is mixed with filter membrane chip,
Separation is difficult.Separation of solid and liquid link is there are three kinds of modes at present, no matter which kind of mode there are problem：
1st, solid-liquid does not separate, and directly by filter membrane fragment and the stainless steel spatula of liquid, scrapes into glass syringe, passes through
0.2 μm of aperture pin type organic phase filter filtering.This method easily blocks filter membrane, and in the case where pressing force is big, there are filter membrane to break
Damage, does not reach the purpose for removing cell and filter membrane chip, in an experiment potassium hydroxide.
2nd, glass syringe Aspirate supernatant is used after centrifuging, is filtered by 0.2 μm of aperture pin type organic phase filter.Extract
For organic phase, to avoid other absorption of material dissolution and tube wall to pigment, centrifuge tube material uses glass or polytetrafluoroethyl-ne
Alkene.Glass centrifuge tube material is fragile, and the centrifugal force born is limited, can not carry out high speed centrifugation, it is usually more 4000 revs/min it
Under, solid-liquid separation effect is bad, frangible, and sample breakage rate is high.Polytetrafluoroethylene (PTFE) centrifuge tube, can carry out high speed centrifugation, solid-liquid
Good separating effect, but it is undesirable in the case where extraction liquid measure is few, and supernatant liquid measure is few, can not draw enough amounts to carry out
Filter, and it is expensive, it is disposable test consumable, it is impossible to reuse.
3rd, extract is directly drawn using stainless steel syringe needle pressing glass syringe, passes through 0.2 μm of aperture pin type organic phase
Filter filters.This method uses syringe needle 16G for animals more, top side opening, and needle point is pressed against chip in 50mm or so by length
On, while extract syringe.This method easily blocks pin hole in a large amount of extracts, and when extracting liquid measure less, easily draw
Too many air, needs repeated multiple times operation to obtain enough extracts and is analyzed.Even with flat mouth 12G syringe needles, still
There are problems that this.
The sample handling processes of existing pigment：After GF/F films after filtering sea are thawed, it is deployed into culture dish, with clear
Filter membrane is cut into 1mm slices by clean stainless steel scissors, and is transferred in container.Add quantitatively organic extraction in a reservoir with liquid-transfering gun
Liquid (1.5~5ml etc.) is taken, after adding quantitative internal standard mother liquor (100~200 μ L are differed) with micro-sampling pin, with stainless
The glass syringe of draw point head gently extrudes filter membrane, is allowed to immerse extract, and container is placed on to the burning for filling frozen water vertically
In cup, it is advisable so that frozen water liquid level is not consistent with height of liquid level inside container, is ultrasonically treated.With 0.2 μm of aperture pin after absorption extract
Formula organic phase filter filters, and filtrate (0.8~2mL or so) is moved in clean sample bottle.Accurately drawn with micro-sampling pin
It is 5 according to filtrate and ultrapure water volume ratio in quantitative filtrate (600~1500 μ L are differed) to 2.0ml liquid-phase inlet bottles:1 ratio
Add quantitative ultra-pure water, after being mixed on turbula shaker immediately on machine testing.It is all these operation in low light environment into
OK.
Utility model content
The purpose of this utility model is exactly to solve the problems of the prior art, proposes a kind of pressing for being used to draw extract
Formula syringe needle, coordinates glass syringe to realize in the case of without other material dissolutions, and more extracts are drawn out in pressing,
To meet the requirement of analysis, the structure of this syringe needle can effectively prevent filter membrane chip from blocking syringe needle, and easy to clean, Ke Yiduo
Secondary use.
To achieve the above object, the utility model proposes a kind of push type syringe needle for being used to draw extract, including pin
Bolt, pin stalk, needle point, chassis, curved support, one end connection syringe of the pintle, the other end and the pin stalk of pintle connect, pin
Bolt and pin stalk are hollow structure；The pintle is equipped with arc recessed area, arc recessed area by curved support connecting base plate, chassis middle part
Towards the outer end on chassis；Pin stalk end is equipped with needle point, the bottom of the edge of needle point close to arc recessed area；Set on the chassis
There are some apertures.
Preferably, the aperture uniform circular is distributed in around arc recessed area, the diameter dimension of aperture is 0.3mm.
Preferably, the chassis is the disk of diameter 4mm, the thickness on chassis is 1mm.
Preferably, the diameter dimension of the arc recessed area is 1.62mm.
Preferably, the pin stalk is exterior to be equipped with curved support, curved support one end is connected on pintle, and curved support is another
One end is connected on chassis.
Preferably, the curved support and the connection mode on pintle, chassis connect for casting connection or jack.
Preferably, the quantity of the curved support is 2, curved support is located at the outer symmetrical distribution of pin stalk.
Preferably, the curved support material is stainless steel.
Preferably, the needle point internal diameter is 1.25mm, needle point is open for contracting point, hatch bore diameter 1mm.
Preferably, the pintle, pin stalk, needle point, chassis material are stainless steel.
The beneficial effects of the utility model：
1st, the respective outer side edges glass syringe of the utility model syringe needle can realize directly draw extract, without early period from
The heart, saves sample treatment cost.
2nd, the respective outer side edges glass syringe of the utility model syringe needle can be realized in the case of without other material dissolutions,
More extracts are drawn out in pressing, to meet the requirement of analysis, can effectively be prevented filter membrane chip from blocking syringe needle, be reduced and obtain
To the number of operations of enough extracts, convenient experimental operation can be made in use, improve work efficiency.
3rd, the resistance to organic reagent of the material of the utility model syringe needle, will not dissolution other materials, the later stage is easy to clean, Ke Yiduo
Secondary use.
The feature and advantage of the utility model will be described in detail by embodiment combination attached drawing.
Brief description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, drawings in the following description are only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, other attached drawings can also be obtained according to these attached drawings.
Fig. 1 is a kind of overall structure diagram for the push type syringe needle for being used to draw extract；
Fig. 2 is a kind of right view for the push type syringe needle for being used to draw extract.
1- pintles in figure；11- curved supports；2- pins obstruct；3- needle points；4- chassis；41- arcs recessed area；42- apertures.
Embodiment
To make the purpose of this utility model, technical solution and advantage clearer, below by the technology of the utility model
Scheme is described in detail.Obviously, the described embodiments are only a part of the embodiments of the utility model, rather than all
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not before creative work is made
Obtained all other embodiment is put, belongs to the scope that the utility model is protected.
As shown in Figure 1, Figure 2, the utility model, including pintle 1, pin stalk 2, needle point 3, chassis 4, curved support 11, the pintle 1
One end connection syringe, the other end of pintle 1 is connected with pin stalk 2, and it is hollow structure that pintle 1 and pin, which obstruct 2,；The pintle 1 is logical
11 connecting base plate 4 of curved support is crossed, the middle part of chassis 4 is equipped with arc recessed area 41, outer end of the arc recessed area 41 towards chassis 4；It is described
Pin obstructs 2 ends and is equipped with needle point 3, the bottom of the edge of needle point 3 close to arc recessed area 41；The chassis 4 is equipped with some apertures 42.
It is preferably dimensioned to be：42 uniform circular of aperture is distributed in around arc recessed area 41, and the diameter dimension of aperture 42 is
0.3mm.The chassis 4 is the disk of diameter 4mm, and the thickness on chassis 4 is 1mm.The diameter dimension of the arc recessed area 41 is
1.62mm。
Chassis size and pore size can be adjusted correspondingly as needed.
2 outside of pin stalk is equipped with curved support 11, and 11 one end of curved support is connected on pintle 1, and curved support 11 is another
One end is connected on chassis 4.
The curved support 11 and the connection mode on pintle 1, chassis 4 connect for casting connection or jack.
Curved support 11 can also use other usual manner to connect.
The quantity of the curved support 11 is 2, and curved support 11 is located at the outside of pin stalk 2.
3 internal diameter of needle point is 1.25mm, and needle point 3 is open for contracting point, hatch bore diameter 1mm.
Material is preferred：11 material of curved support is stainless steel.The pintle 1, pin stalk 2, needle point 3,4 material of chassis are equal
For stainless steel.
Can also use other materials, or part uses other materials, as long as can holding structure hardness, it is and resistance to
The material of organic reagent corrosion.
First embodiment：
Pintle 1, pin stalk 2, needle point 3 and the chassis 4 of hollow stainless steel, 1 part of pintle increase, two curved supports 11, one end
It is cast on pintle 1, one end is cast on chassis 4, the purpose is to the load in pressing, protects pin stalk 2 not bend and deform.
3 internal diameter of needle point is 1.25mm, its needle point 3 is contracting point opening, hatch bore diameter 1mm, and edge is close to arc recessed area 41
Bottom, to be drawn to the extract of convergence as far as possible.
Chassis 4 is the circle of 4mm diameters, stainless steel, thickness 1mm, and there is the arc recessed area 41 of a diameter of 1.62mm in centre,
Other regions are covered with the aperture 42 of a diameter of 0.3mm.Arc recessed area 41 be in order to collect the extract from 42 discharge by pressing of aperture,
The 42 block film chip of aperture of 0.3mm enters collecting region and blocks syringe needle opening, and chassis 4 is met extrudes extract by press mold chip
Purpose.
Utility model works process：
During the work time, it is a kind of push type syringe needle for drawing extract of the utility model using process：Will
The upper syringe of this syringe needle connection, whole syringe needle is slowly pressed into sample bottle, while pull syringe stem to draw extract, chassis
4 are extruded to needle wall by press mold chip extrusion extract, extract from aperture 42, and flowing is pooled to the arc inside chassis 4
Shape recessed area 41.As extract quantitative change is few, pressing strength is constantly aggravated, more extracts is flowed out from filter membrane chip, pin
Point 3, easy to draw extract, pulls syringe stem to draw the extract for flowing into arc recessed area 41 close to arc recessed area 41.0.3mm
42 block film chip of aperture enter collecting region block syringe needle opening；Curved support 11 can ensure during pressing that pin obstructs 2
Do not bend and deform, drawing extract can be normally carried out.
The utility model, the respective outer side edges syringe of this syringe needle can be realized in the case of without other material dissolutions, pressed
Pressure draws out more extracts, to meet the requirement of analysis, can effectively prevent filter membrane chip from blocking syringe needle, reduction is got
The number of operations of enough extracts, can make convenient experimental operation in use, improve work efficiency.The material of syringe needle
Resistance to organic reagent, will not dissolution other materials, the later stage is easy to clean, can be used for multiple times.
The above, is only specific embodiment of the present utility model, but the scope of protection of the utility model is not limited to
In this, any one skilled in the art can readily occur in change in the technical scope that the utility model discloses
Or replace, it should be covered within the scope of the utility model.Therefore, the scope of protection of the utility model should be with the power
Subject to the protection domain that profit requires.
Claims (10)
- A kind of 1. push type syringe needle for being used to draw extract, it is characterised in that：Including pintle (1), pin stalk (2), needle point (3), Chassis (4), curved support (11), one end connection syringe of the pintle (1), the other end and the pin stalk (2) of pintle (1) connect Connect, pintle (1) and pin stalk (2) are hollow structure；The pintle (1) passes through curved support (11) connecting base plate (4), chassis (4) Middle part is equipped with arc recessed area (41), the outer end of arc recessed area (41) towards chassis (4)；Described pin stalk (2) end is equipped with needle point (3), bottom of the edge of needle point (3) close to arc recessed area (41)；The chassis (4) is equipped with some apertures (42).
- A kind of 2. push type syringe needle for being used to draw extract as claimed in claim 1, it is characterised in that：The aperture (42) Uniform circular is distributed in around arc recessed area (41), and the diameter dimension of aperture (42) is 0.3mm.
- A kind of 3. push type syringe needle for being used to draw extract as claimed in claim 1, it is characterised in that：The chassis (4) For the disk of diameter 4mm, the thickness of chassis (4) is 1mm.
- A kind of 4. push type syringe needle for being used to draw extract as claimed in claim 1, it is characterised in that：The arc recessed area (41) diameter dimension is 1.62mm.
- A kind of 5. push type syringe needle for being used to draw extract as claimed in claim 1, it is characterised in that：The pin obstructs (2) Outside is equipped with curved support (11), and curved support (11) one end is connected on pintle (1), and curved support (11) other end is connected to On chassis (4).
- A kind of 6. push type syringe needle for being used to draw extract as claimed in claim 5, it is characterised in that：The curved support (11) connection mode with pintle (1), chassis (4) connects for casting connection or jack.
- A kind of 7. push type syringe needle for being used to draw extract as claimed in claim 5, it is characterised in that：The curved support (11) quantity is 2, and curved support (11) is located at the outer symmetrical distribution of pin stalk (2).
- A kind of 8. push type syringe needle for being used to draw extract as claimed in claim 5, it is characterised in that：The curved support (11) material is stainless steel.
- A kind of 9. push type syringe needle for being used to draw extract as claimed in claim 1, it is characterised in that：The needle point (3) Internal diameter is 1.25mm, and needle point (3) is open for contracting point, hatch bore diameter 1mm.
- A kind of 10. push type syringe needle for being used to draw extract as claimed in claim 1, it is characterised in that：The pintle (1), pin stalk (2), needle point (3), chassis (4) material are stainless steel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720860018.8U CN207263476U (en) | 2017-07-17 | 2017-07-17 | A kind of push type syringe needle for being used to draw extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720860018.8U CN207263476U (en) | 2017-07-17 | 2017-07-17 | A kind of push type syringe needle for being used to draw extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207263476U true CN207263476U (en) | 2018-04-20 |
Family
ID=61926946
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720860018.8U Expired - Fee Related CN207263476U (en) | 2017-07-17 | 2017-07-17 | A kind of push type syringe needle for being used to draw extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207263476U (en) |
-
2017
- 2017-07-17 CN CN201720860018.8U patent/CN207263476U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180420 Termination date: 20200717 |