CN207488048U - Flow cytometer sample preparation cell filter - Google Patents

Abstract

The utility model provides a cell filter for flow cytometer sample preparation which can improve sample preparation efficiency. A cell filter for flow cytometer sample preparation, comprising a cylinder body and a filter screen connected to one end of the cylinder body and a filter screen fixing ring, the filter screen fixing ring is sleeved on the outer wall of the cylinder body, and the inner diameter of the filter screen fixing ring is larger than the cylinder The outer diameter of the outer body and the outer wall of the filter fixing ring are provided with a number of grooves parallel to the axial direction of the cylinder. The filter is fixed to the end of the cylinder through the filter fixing ring. The aperture of the filter is not more than 80 mesh. The cell filter for flow cytometer sample preparation of the utility model can be conveniently used in conjunction with the centrifuge tube, and can directly filter the preliminary prepared flow cytometer analysis sample into the centrifuge tube, which improves the efficiency of sample preparation and reduces loss of samples and reagents.

Description

Cell Strainers for Sample Preparation for Flow Cytometry

technical field

The utility model relates to a filtering device, in particular to a cell filter.

Background technique

Flow cytometry is an important analytical instrument, widely used in medicine, biology and agronomy and other fields. When isolating cells, nuclei, or other organelles from multicellular organisms for flow cytometric analysis, it is often necessary to filter the sample to remove large particles of impurities. The filtered solution containing the desired cells or organelles generally needs to be centrifuged to collect the desired cells or organelles.

The current cell filter cannot directly filter the filtrate into a small centrifuge tube, which makes the operation process of filtration and centrifugation inconvenient, affects the efficiency of sample preparation, and also causes sample loss. The cells or nuclei of higher animals and plants vary in size and shape, and the diameter is generally 5-20 μm. Ordinary disposable filters (generally 0.22 μm or 0.45 μm) will retain experimental target cells due to their small pore size; while traditional cotton yarn filters require a large amount of experimental samples due to the adsorption of samples, which is not suitable for some small sample sizes in scientific research. Analysis of precious samples.

Utility model content

The purpose of this utility model is to provide a flow cytometer sample preparation cell filter that can improve the sample preparation efficiency, which can be directly placed on the centrifuge tube for operation and directly filter the biological sample into the centrifuge tube for the next step The centrifugal operation solves the problems raised in the above-mentioned background technology.

The technical scheme that the utility model solves its technical problem adopts is:

A cell filter for flow cytometer sample preparation, comprising a cylinder body and a filter screen connected to one end of the cylinder body and a filter screen fixing ring, the filter screen fixing ring is sleeved on the outer wall of the cylinder body, and the inner diameter of the filter screen fixing ring is larger than the cylinder The outer diameter of the outer body and the outer wall of the filter fixing ring are provided with a number of grooves parallel to the axial direction of the cylinder. The filter is fixed to the end of the cylinder through the filter fixing ring. The aperture of the filter is not more than 80 mesh. Ventilation holes are formed between the groove on the outer wall of the filter fixing ring and the centrifuge tube containing the filtrate, so that the filtrate in the cell filter can flow into the centrifuge tube smoothly; the pore size of the filter can be selected according to the size of the target object to be separated , because the samples analyzed by flow cytometer generally need to be fluorescently stained, and the influence of impurities in the samples can be eliminated by software, so in order to improve the filtration efficiency and ensure the filtration quality, the pore size of the filter should not exceed 80 mesh.

Preferably, the cylinder is made of plastic or metal. This allows for adaptation to different sterilization methods.

Preferably, the inner diameter of the opening of the end of the cylinder connected with the filter screen is smaller than the inner diameter of the opening of the other end of the cylinder. This can facilitate the liquid addition operation.

Preferably, the filter screen is a metal filter screen. The metal filter screen can increase the sample filtration speed and reduce the adsorption of samples, and facilitates autoclaving.

Preferably, the filter screen fixing ring is a torus made of plastic or metal, the inner diameter of one end of the torus matches the outer diameter of the cylinder at the connecting part, and the inner diameter of the other end of the torus is smaller than that of the filter screen outside diameter. The outer diameter of the filter fixing ring is slightly smaller than the inner diameter of the centrifuge tube mouth used to hold the filtrate, so that the cell filter for flow cytometry sample preparation can be inserted and stably placed on the centrifuge tube.

The beneficial effects of the utility model are:

The cell filter for flow cytometer sample preparation of the utility model can be conveniently used in conjunction with the centrifuge tube, and can directly filter the preliminary prepared flow cytometer analysis sample into the centrifuge tube, which improves the efficiency of sample preparation and reduces loss of samples and reagents.

Description of drawings

Fig. 1 is the structural representation of the utility model;

Fig. 2 is the use schematic diagram of the utility model.

In the figure: 1. cylinder body, 2. filter screen, 3. filter screen fixing ring, 4. groove, 5. centrifuge tube, 6. centrifuge tube holder.

Detailed ways

The technical solutions of the present utility model will be further specifically described below through specific embodiments in conjunction with the accompanying drawings. It should be understood that the implementation of the utility model is not limited to the following embodiments, and any modifications and/or changes made to the utility model will fall within the protection scope of the utility model.

Example:

A cell filter for flow cytometer sample preparation as shown in Figure 1, including a cylinder 1, a filter 2 connected to one end of the cylinder and a filter fixing ring 3, the cylinder is a cylinder made of metal , The inner diameter of the opening of the end of the cylinder connected with the filter screen is smaller than the inner diameter of the opening of the other end of the cylinder, which can facilitate the operation of adding liquid.

The filter screen is fixed at the end of the cylinder through the filter screen fixing ring, and the aperture of the filter screen is not more than 80 mesh. The filter screen is a metal filter screen, which can increase the sample filtration speed and reduce the adsorption of the sample, and is conducive to high-pressure sterilization. The pore size of the filter can be selected according to the size of the target object to be separated. Since the samples analyzed by the flow cytometer generally need to be fluorescently stained, the influence of impurities in the samples can be eliminated by software. Therefore, in order to improve the filtration efficiency and ensure the filtration quality, the filter The aperture of the mesh should not exceed 80 mesh.

The filter fixing ring is sleeved on the outer wall of the cylinder. The inner diameter of the filter fixing ring is larger than the outer diameter of the cylinder. The outer wall of the filter fixing ring is provided with a number of grooves 4 parallel to the axial direction of the cylinder. Ventilation holes are formed between the cells so that the filtrate in the cell strainer can flow smoothly into the centrifuge tube. The filter screen fixing ring is a torus made of metal, the inner diameter of one end of the torus matches the outer diameter of the cylinder at the connecting part, and the inner diameter of the other end of the torus is smaller than the outer diameter of the filter screen. The outer diameter of the filter fixing ring is slightly smaller than the inner diameter of the centrifuge tube mouth used to hold the filtrate, so that the cell filter for flow cytometer sample preparation can be inserted and stably placed on the centrifuge tube.

This embodiment is applied to the preparation of flow cytometry nuclei samples from plant materials for filtering plant materials cut by blades. The rationale for isolating intact nuclei from plant tissues for genome size determination, ploidy analysis, and cell cycle studies is that, according to published literature, plant material such as leaves can be soaked in a buffered solution with surfactants, Cut the plant tissue with a sharp blade, and the cells at the incision can release their complete nuclei under the action of this buffer solution. The released nuclei are purified by filtration, centrifugation, etc., and then stained with a fluorescent dye (such as PI) that can specifically bind to DNA molecules for flow cytometry analysis. Flow cytometry measures the fluorescence intensity of each particle. Since the fluorescence intensity is directly proportional to the content of DNA, the relative content of DNA in each particle can be calculated according to the measured fluorescence intensity. Through statistical analysis and comparison with standard samples, results such as genome size, chromosomal ploidy of unknown samples, and the proportion of cells with different cell cycles were finally obtained.

As shown in Figure 2, when in use, several 1.5ml centrifuge tubes 5 are placed on the centrifuge tube holder 6, the cell filter for flow cytometer sample preparation of the present embodiment is placed on the centrifuge tube, and the pipette Aspirate the liquid to be filtered and pour it into the cell strainer, and the liquid containing the nucleus flows directly into the centrifuge tube below under the action of gravity. This embodiment is especially suitable for processing samples in batches, which can greatly improve the efficiency of sample preparation and reduce the loss of samples and reagents.

The above-described embodiment is only a preferred solution of the utility model, and does not limit the utility model in any form. There are other variations and improvements without exceeding the technical solution described in the claims. type.

Claims (9)

1. A cell filter for flow cytometer sample preparation, characterized in that: the cell filter for flow cytometer sample preparation includes a cylinder body and a filter screen and a filter screen fixed ring connected to one end of the cylinder body, the filter screen The mesh fixing ring is sleeved on the outer wall of the cylinder. The inner diameter of the filter fixing ring is larger than the outer diameter of the cylinder. The outer wall of the filter fixing ring is provided with a number of grooves parallel to the axial direction of the cylinder. The filter is fixed on the end of the cylinder through the filter fixing ring. The pore size of the filter screen is not greater than 80 mesh. 2 . The cell filter for flow cytometer sample preparation according to claim 1 , wherein the cylinder is made of plastic or metal. 3 . 3. A cell filter for flow cytometer sample preparation according to claim 1 or 2, characterized in that: the inner diameter of the opening of the end of the cylinder connected to the filter screen is smaller than that of the other end of the cylinder. inner diameter of the opening. 4. A cell filter for flow cytometer sample preparation according to claim 1 or 2, characterized in that: said filter is a metal filter. 5 . A cell filter for flow cytometer sample preparation according to claim 3 , wherein the filter screen is made of metal. 6 . 6. A cell filter for flow cytometer sample preparation according to claim 1 or 2, characterized in that: the filter screen fixing ring is a ring made of plastic or metal, and the ring at one end of the ring is The inner diameter of the end matches the outer diameter of the cylinder at the joint, and the inner diameter of the other end of the torus is smaller than the outer diameter of the filter screen. 7. A cell filter for flow cytometer sample preparation according to claim 3, characterized in that: the filter fixing ring is a plastic or metal torus, and the end of one end of the torus is The inner diameter matches the outer diameter of the cylinder at the joint, and the inner diameter of the other end of the torus is smaller than the outer diameter of the filter screen. 8. A cell filter for flow cytometer sample preparation according to claim 4, characterized in that: the filter fixing ring is a plastic or metal torus, and the end of one end of the torus is The inner diameter matches the outer diameter of the cylinder at the joint, and the inner diameter of the other end of the torus is smaller than the outer diameter of the filter screen. 9. A cell filter for flow cytometer sample preparation according to claim 5, characterized in that: said filter screen fixing ring is a ring made of plastic or metal, and the end of one end of the ring is The inner diameter matches the outer diameter of the cylinder at the joint, and the inner diameter of the other end of the torus is smaller than the outer diameter of the filter screen.