CN213113315U - Animal tissue broken liquid component separating tube - Google Patents

Abstract

The utility model provides an animal tissue broken liquid component separation tube solves the animal tissue broken liquid and draws the loaded down with trivial details multistep operation flow of specific component in-process, improves work efficiency, reduces the contamination probability, improves cell activity. An animal tissue crushing liquid component separation tube comprises a cover, a tube body and a separation structure; the separation structure comprises a grid and a mesh screen, and the pore diameter of the grid is larger than that of the mesh screen; the body is including dismantling the application of sample section of connection and collection section, application of sample section upper end uncovered and with the lid cooperation, the lower extreme is equipped with the mesh screen, the application of sample section divide into through the net first hold the chamber and the chamber is held to the second, the collection section has the third for the uncovered bottom confined inside in upper end and holds the chamber, the third holds and sets up multistage density gradient separating medium in the chamber, keep apart through the inertia separation glue between the density gradient separating medium at different levels, first hold the chamber, the second holds chamber and the third and holds the chamber intercommunication.

Description

Animal tissue broken liquid component separating tube

Technical Field

The utility model relates to an animal tissue broken fluid component separation pipe belongs to animal tissue separation and retrieves experimental apparatus technical field.

Background

Cells are the fundamental units that make up biological structures and functions. In an animal, cells are differentiated to form a plurality of cell populations with different morphologies, structures and functions, and the cell populations with similar morphologies, structures and functions are called tissues. Organs are composed of several different tissues, which are combined in a certain order to form a structure with certain functions.

Currently, with the continuous development of technologies in the field of life science, the exploration of the structure, life mechanism and disease origin of organisms by using cells and molecules as research objects has become the mainstream means for the research and development of innovative processes.

In a general animal cell isolation and culture experiment, in addition to blood cells, in the isolation and culture experiment of cells at other parts, a small amount of tissue at the part is collected firstly, and then single cells are formed by mechanical shearing, enzymolysis or combination of the two means. Then, pretreating through multi-step operations such as centrifugation, sieving, cleaning and the like to obtain a single cell suspension, further enriching target cells through processes such as red blood cell lysis, flow sorting, magnetic bead screening and the like, and finally, carrying out pure culture on the target cells by using a specific culture medium.

The technical defects are as follows: the process is tedious and long, the cells are exposed to the environment by opening the container for many times, the activity of the cells is greatly reduced, and the probability of contamination is increased.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an animal tissue broken fluid component separation tube solves the animal tissue broken fluid and draws the loaded down with trivial details multistep operation flow of specific component in-process, improves work efficiency, reduces the contamination probability, improves cell activity.

The utility model discloses a realize above-mentioned purpose, realize through following technical scheme:

an animal tissue crushing liquid component separation tube comprises a cover, a tube body and a separation structure; the separation structure comprises a grid and a mesh screen, and the pore diameter of the grid is larger than that of the mesh screen; the body is including dismantling the application of sample section of connection and collection section, application of sample section upper end uncovered and with the lid cooperation, the lower extreme is equipped with the mesh screen, the application of sample section divide into through the net first hold the chamber and the chamber is held to the second, the collection section has the third for the uncovered bottom confined inside in upper end and holds the chamber, the third holds and sets up multistage density gradient separating medium in the chamber, keep apart through the inertia separation glue between the density gradient separating medium at different levels, first hold the chamber, the second holds chamber and the third and holds the chamber intercommunication.

On the basis of the animal tissue crushing liquid component separation tube, the grid is made of polypropylene.

On the basis of the animal tissue crushing liquid component separation tube, the mesh screen is made of stainless steel.

On the basis of the animal tissue crushing liquid component separation tube, the outer surface of the cover is of an anti-skid texture structure.

On the basis of the animal tissue disruption liquid component separation tube, the total length of the tube body is 115-125mm, and the outer diameter is 15-30 mm.

On the basis of the animal tissue crushing liquid component separation tube, the tail end of the collecting section is of a cone-shaped structure.

The utility model discloses a theory of operation: the density gradient separation liquid and the inert separation glue are adopted to realize the clear separation of components with different specific gravities, and the specific gravities are as follows: high specific gravity cells, inert separation gel, density gradient separation liquid, low specific gravity cells and tissue fluid; and the density gradient separation liquid and the inert separation gel do not influence the cell activity or the molecular activity of each component, the low specific gravity single cells float on the surface of the density gradient separation liquid, the enrichment is effective, the suction is easy, the volume is small, the cell density is high, the cell recovery rate is high, and the cleaning of the cells is convenient.

The utility model has the advantages that: the separation tube is designed in a split mode, after centrifugation is finished, the upper section of waste tissue residues are screwed out, and available components such as single cells, tissue fluid and the like are sucked from the lower section; the separation of each component of the tissue disruption solution is realized by one-time centrifugation, and the problems of bacteria contamination and cell activity reduction caused by multiple uncapping and long operation time are avoided. Meanwhile, the single cell recovery rate is high, the collection volume is small, and the subsequent cleaning and culture are convenient.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention.

Fig. 1 is an external view of an embodiment of the present invention.

Fig. 2 is a schematic cross-sectional view of an embodiment of the present invention.

Fig. 3 is a schematic diagram after sample loading according to an embodiment of the present invention.

Fig. 4 is a schematic diagram of the end of centrifugation according to the embodiment of the present invention.

1. The device comprises a tube cover, 2 parts of a sample adding section, 3 parts of a collecting section, 21 parts of a gasket, 22 parts of threads, 23 parts of a grid, 24 parts of a mesh screen, 31 parts of an inert separation gel, 32 parts of a density gradient separation liquid, A parts of a tissue crushing liquid, A1 parts of a large tissue block, blood vessels and connective tissues, A2 parts of a small tissue block, A3 parts of a tissue liquid, A4 parts of low specific gravity cells and A5 parts of high specific gravity cells.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate directions

The positional or positional relationship is based on that shown in the drawings and is for convenience of description and simplicity of illustration only

Rather than indicating or implying that the device or element so referred to must have a particular orientation, be constructed and arranged in a particular orientation

Operation, and therefore should not be construed as limiting the invention. Furthermore, "first," "second," "third," and "fourth" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Referring to fig. 1 and 2, an animal tissue breaking solution component separating tube comprises a cover 1, a tube body and a separating structure; the separation structure comprises a mesh 23 and a mesh 24, wherein the mesh 23 has larger pore size than the mesh 24; the pipe body comprises a sample adding section 2 and a collecting section 3 which are detachably connected, and the sample adding section 2 and the collecting section 3 can be actually connected by threads 22 and sealed by a sealing ring 21 or connected in an inserting way; open and with 1 screw-thread fit of lid in 2 upper ends in application of sample section, the lower extreme is equipped with mesh screen 24, application of sample section 2 divide into through net 23 and firstly holds the chamber and the chamber is held to the second, it has the third for the open bottom confined inside in upper end to collect section 3 and holds the chamber, firstly hold the chamber, the second holds chamber and the third and holds the chamber intercommunication, the third holds and sets up inertia separation glue 31 and density gradient parting fluid 32 in the chamber, the demonstration in the embodiment picture is the condition of only putting a density gradient parting fluid 32, can set up multistage density gradient parting fluid 32 in the chamber is held to the third in reality, keep apart through inertia separation glue 31 between the density gradient parting fluid 32 at different levels, can realize the further separation of different proportion cells.

The embodiment of the utility model provides a, 23 materials of net are polypropylene, and net 23 blocks bold tissue, connective tissue and blood vessel, prevents that it from pasting in mesh screen 24.

The embodiment of the utility model provides a, 24 materials of mesh screen are stainless steel.

The embodiment of the utility model provides a, 1 surface of lid is anti-skidding line structure.

The embodiment of the utility model provides a, body total length 115- & ltwbr & gt 125mm, external diameter 15-30 mm.

The embodiment of the utility model provides a, 3 ends of collection section are the taper structure.

The utility model discloses the working process is as follows: referring to fig. 3 and 4, adding a tissue fluid a of a broken animal into a first containing cavity at the upper part of a centrifuge tube, preparing an inert separation gel 31 at the bottom end inside a collecting section 3, screwing and connecting density gradient separation fluids 32, 2 and 3 through a thread 22, sealing the separation tube through a sealing ring 21, placing the separation tube in a centrifuge, centrifuging at a proper rotating speed (the rotating speed depends on the broken degree of the broken fluid and the viscosity of the fluid, and if the viscosity is too high, the separation tube is properly diluted by buffer solution such as physiological saline and the like), separating components of the tissue broken fluid after centrifugation, leaving a tissue block a1 (large tissue block, connective tissue and blood vessel) at the bottom of the first containing cavity, leaving a small tissue block a2 at the bottom of the second containing cavity, tissue fluid A3, low specific gravity cells a4 (lymphocytes, monocytes, stem cells, progenitor cells and the like), density gradient separation fluids 32, inert separation gel 31, high specific gravity cells a5 (erythrocytes, Neutrophils, etc.) are layered from top to bottom in the third containing cavity, the sample adding section 2 is taken down by rotating the screw thread between the sample adding section 2 and the collecting section 3, and a pipettor or a suction pipe is inserted into the position of the required component to absorb the component, thereby facilitating the operations of culturing, detecting, etc. of the following components.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (6)

1. An animal tissue crushing liquid component separating tube is characterized in that: comprises a cover (1), a pipe body and a separation structure; the separation structure comprises a mesh (23) and a mesh screen (24), wherein the mesh (23) has larger pore size than the mesh screen (24); the body is including sample loading section (2) and the collection section (3) of dismantling the connection, open and the cooperation with lid (1) in sample loading section (2) upper end, the lower extreme is equipped with mesh screen (24), sample loading section (2) divide into through net (23) that first hold the chamber and the second holds the chamber, collection section (3) have the third for the open bottom confined inside in upper end and hold the chamber, the third holds and sets up multistage density gradient parting fluid in the chamber, keep apart through the inertia separation gel between the density gradient parting fluid at different levels, first hold the chamber, the second holds chamber and the third and holds the chamber intercommunication.

2. The animal tissue disruption solution component-separating tube according to claim 1, wherein: the material of the grid (23) is polypropylene.

3. The animal tissue disruption solution component-separating tube according to claim 1, wherein: the mesh screen material is stainless steel.

4. The animal tissue disruption solution component-separating tube according to claim 1, wherein: the outer surface of the cover (1) is of an anti-skid texture structure.

5. The animal tissue disruption solution component-separating tube according to claim 1, wherein: the total length of the tube body is 115 mm and 125mm, and the outer diameter is 15-30 mm.

6. The animal tissue disruption liquid component-separating tube according to any one of claims 1 to 5, wherein: the end of the collecting section (3) is of a cone-shaped structure.

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