CN210103942U

CN210103942U - Device for conveniently collecting exfoliated cells in large amount of body fluid

Info

Publication number: CN210103942U
Application number: CN201920545553.3U
Authority: CN
Inventors: 任玉波; 贺青; 田凤兰; 刘宇
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-04-22
Filing date: 2019-04-22
Publication date: 2020-02-21
Anticipated expiration: 2029-04-22

Abstract

The utility model relates to a device of cell that drops in convenient collection a large amount of body fluids, including the sharp bottom cell receiving flask that is equipped with cell elution preservative fluid, its characterized in that: the device for conveniently collecting the cast-off cells in a large amount of body fluid consists of a specimen preservation container and a liquid inlet cavity on the upper part, a liquid discharge cavity with a conical cell collection filter membrane and a sealing cover on the lower end opening on the lower part, and a sharp-bottomed cell collection bottle filled with cell elution preservation liquid. The utility model discloses a method and device easy operation, its lower part flowing back chamber can be fast convenient mutual equipment and dismantlement through the intercommunication interface with upper portion feed liquor chamber and sharp bottom cell receiving flask, easily collects the cell that drops in a large amount of body fluids, simplifies the acquisition method to the cell in the body fluid by a wide margin.

Description

Device for conveniently collecting exfoliated cells in large amount of body fluid

Technical Field

The utility model relates to a how conveniently collect the cell that breaks away from in a large amount of body fluids to can be quick will acquire cell elution and save, carry out subsequent centrifugation and acquire cell aggregation granule or carry out smear dyeing processing.

Background

How to obtain the exfoliated cells contained in the body fluid is that many methods such as sedimentation method, centrifugation method, filtration method and the like are available at present, and these methods are generally only applicable to body fluid samples with small sample amount and high cell content in the samples, but for body fluids with large body fluid amount and low cell content, no effective and convenient method is available at present for rapid treatment, the exfoliated cells in the body fluids are obtained, and are recovered and stored for the subsequent medical inspection of the pathological examination department.

Disclosure of Invention

In order to overcome the defects in the prior art, the utility model provides a device for conveniently collecting the cast-off cells in a large amount of body fluid

The utility model discloses a device of cell that drops in convenient collection a large amount of body fluids, including the sharp bottom cell receiving flask that is equipped with cell elution preservative fluid, its characterized in that: the device for conveniently collecting the cast-off cells in a large amount of body fluid consists of a specimen preservation container and a liquid inlet cavity on the upper part, a liquid discharge cavity with a conical cell collection filter membrane and a sealing cover on the lower end opening on the lower part, and a sharp-bottomed cell collection bottle filled with cell elution preservation liquid.

Preferably, the lower liquid discharge cavity of the device for conveniently collecting the exfoliated cells in a large amount of body fluid can be rapidly and conveniently assembled and disassembled with the upper liquid inlet cavity and the sharp-bottomed cell collecting bottle through intercommunicating interfaces, and the device is in sealing connection through a clamping buckle, a thread, an insertion connection or an adhesion connection and the like.

Preferably, the pore size of the conical cell collection filter is 0.01 μm to 1 mm.

Preferably, the internal angle of the vertex angle of the conical cell collection filter is 20-270 degrees.

Preferably, the conical cell collection filter has an effective surface area of 1 cm to 100 cm.

Preferably, the lower drainage port of the drainage cavity is provided with a sealing cover, and after the sealing cover is hermetically connected with the lower drainage port, the whole body consisting of the drainage cavity and the sharp-bottomed cell collecting bottle can be opened only in a destructive manner.

Preferably, the device for conveniently collecting the exfoliated cells in the large amount of body fluid can be made of metal, plastic, glass, sintering materials and the like.

Adopt the technical scheme of the utility model, can gain following beneficial effect:

1. the utility model discloses technical scheme can fully handle the body fluid sample that volume is big, can once only rely on the sample self gravity and the body fluid of liquid in the short time to wash away by the top downwards at the in-process that flows downwards to conical cell collection filter membrane, carry out the separation processing of body fluid and cell, and do not need the help of other auxiliary assembly, until collecting the cell of enough demand, has saved a large amount of manpower and materials for the collection of sample draws.

2. The utility model discloses technical scheme's lower part flowing back chamber can pass through the interface quick exchange assembly and disassembly with upper portion feed liquor chamber and sharp bottom cell receiving flask, like this, when sufficient cell is collected on the surface of cell collection filter membrane, can quick separation upper portion feed liquor chamber and lower part flowing back chamber, assemble sharp bottom cell receiving flask upper portion with lower part flowing back chamber, and make the whole cell elution preservation liquid that soak in sharp bottom cell receiving flask of conical cell filter membrane, protect and preserve the cell of collecting, avoid the disintegration and the corruption of cell.

3. The collected cells are vibrated and eluted in a sharp-bottomed cell collecting bottle, are fused in an elution preservation solution, and are gradually converged to the sharp bottom when standing, so that the bottom cell aggregate is easy to take out, and the realization condition that as much information is obtained in centrifugation or picture detection at the later stage is ensured.

4. The utility model discloses technical scheme simple structure, convenient operation, convenient to use person uses, has solved the difficult problem that a large amount of body fluid specimens are difficult for whole processing and in a large amount of body fluid specimens trace cell can all collect and provide later stage pathological examination's possibility, improves the treatment effeciency.

Drawings

Fig. 1 is a schematic cross-sectional view of an apparatus for conveniently collecting exfoliated cells from a large amount of body fluid according to the present invention.

1-upper port of liquid inlet cavity; 2-liquid inlet cavity; 3-top angle; 4-cell collection filter membrane; 5-a liquid discharge cavity; 6-lower port of the liquid discharge cavity; 7-sealing cover; 8-a sharp-bottomed cell collection bottle; 9-cell elution preservation solution; 10-an interchangeable interface; 11-specimen storage container

Detailed Description

The present invention will be further explained with reference to the following embodiments. Of course, the following detailed description is merely illustrative of various aspects of the invention and should not be taken as limiting the scope of the invention.

Examples

As shown in figure 1, the device for conveniently collecting the cast-off cells in a large amount of body fluid comprises an upper port 1 of a liquid inlet cavity, a liquid inlet cavity 2, a vertex angle 3, a cell collecting filter membrane 4, a liquid discharging cavity 5, a lower port 6 of the liquid discharging cavity, a sealing cover 7, a sharp-bottomed cell collecting bottle 8, a cell eluting and preserving solution 9 and an interchangeable interface 10; a specimen storage container 11; when the cell collection filter membrane is used, the proper area of the effective surface area of the cell collection filter membrane in 1-100 square centimeters and the proper pore size of the cell collection filter membrane in 0.01 micrometer-1 millimeter are selected according to the properties of the sample to process the sample.

Example 1

The method for collecting and processing the cells of the large-volume body fluid specimen by using the device for conveniently collecting the cast-off cells in the large-volume body fluid comprises the following operations;

step 1, adding a body fluid sample needing to be collected by exfoliated cells through an external liquid inlet of a body fluid sample preservation container 11.

And 2, connecting the liquid discharge cavity 5 and the liquid inlet cavity 2 into a whole through the interchange interface 10.

And 3, lifting the body fluid specimen preservation container 11, enabling the body fluid specimen to flow to the conical cell filter membrane 4 under the self gravity, washing the conical cell filter membrane 4 from top to bottom, enabling cells in the specimen to be reserved on the filter membrane, and discharging waste liquid out of the liquid discharge cavity 5.

And 4, separating the liquid inlet cavity 2 from the liquid outlet cavity 5 after the body fluid specimen is completely discharged out of the liquid outlet cavity 5 or when a sufficient amount of cells are collected.

And 5, quickly installing the liquid drainage cavity 5 on the upper part of the cell collection bottle 8 with the pointed bottom through the interchange interface 10 to form a whole, and hermetically connecting the lower port 6 of the liquid drainage cavity with the sealing cover 7 to prevent the cell elution preservative solution 9 from leaking.

And 6, after the drainage cavity 5 is connected with the cell collecting bottle 8 with the pointed bottom, the surface of the conical cell collecting filter membrane 4 with the cells is completely soaked in the cell eluting and preserving solution 9, and the cells collected on the conical cell collecting filter membrane 4 can be eluted into the cell eluting and preserving solution 9 by means of shaking or ultrasound for subsequent detection and analysis.

The utility model discloses a method of cell that drops in convenient collection a large amount of body fluids can fully handle the body fluid sample that the volume is big and cell content is few, and does not need other auxiliary assembly's help, has saved a large amount of manpower and materials for the collection extraction of sample.

The utility model discloses a method of cell that drops in convenient collection a large amount of body fluids, owing to the design has sharp bottom cell receiving flask, can utilize the method of natural settlement to make it gather in the bottom of bottle to the cell that breaks away from in the body fluid of collecting, acquisition that can be convenient provides probably for later stage detection, analysis obtain the improvement of accuracy result.

The utility model discloses a device of cell drops in convenient collection a large amount of body fluids, simple structure, convenient operation, convenient to use person uses, saves the time of handling a large amount of body fluid specimens, improves the treatment effeciency.

Although the present invention has been described and illustrated in detail with reference to specific embodiments thereof, it should be understood that various equivalent changes and modifications can be made therein in accordance with the present invention, and the functional effects thereof are not beyond the spirit of the present invention.

Claims

1. The utility model provides a device of cell that drops in convenient collection bulk body fluid, includes the sharp bottom cell collecting flask that is equipped with cell elution preservative fluid, its characterized in that: the device for conveniently collecting the cast-off cells in a large amount of body fluid consists of a specimen preservation container and a liquid inlet cavity on the upper part, a liquid discharge cavity with a conical cell collection filter membrane and a sealing cover on the lower end opening on the lower part, and a sharp-bottomed cell collection bottle filled with cell elution preservation liquid.

2. The device of claim 1, wherein the device is adapted to facilitate collection of exfoliated cells from a plurality of bodily fluids, and wherein: the liquid discharge cavity at the lower part can be quickly and conveniently detached and connected with the bottom cell collecting bottle filled with cell eluting and preserving liquid and the liquid inlet cavity at the upper part through the intercommunicating interfaces.

3. The device of claim 1, wherein the device is adapted to facilitate collection of exfoliated cells from a plurality of bodily fluids, and wherein: the effective volume of the sharp-bottomed cell collecting bottle filled with the cell eluting and preserving solution is 10 ml-200 ml.

4. The device of claim 1, wherein the device is adapted to facilitate collection of exfoliated cells from a plurality of bodily fluids, and wherein: the aperture of the conical cell collecting filter membrane arranged in the lower liquid discharge cavity of the device capable of collecting the cast-off cells in a large amount of body fluid is 0.01 micron to 1 millimeter.

5. The device of claim 1, wherein the device is adapted to facilitate collection of exfoliated cells from a plurality of bodily fluids, and wherein: the top angle of the conical cell collection filter membrane arranged in the drainage cavity at the lower part of the device capable of collecting the cast-off cells in a large amount of body fluid is 20-270 degrees.

6. The device of claim 1, wherein the device is adapted to facilitate collection of exfoliated cells from a plurality of bodily fluids, and wherein: after the cell collection bottle with the cell elution preservation solution is connected with the liquid drainage cavity with the conical cell collection filter membrane at the lower part, the cell collection filter membrane is soaked in the cell elution preservation solution in the cell collection bottle with the sharp bottom.