KR101035427B1 - Filter container for extracting cells - Google Patents

Filter container for extracting cells Download PDF

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KR101035427B1
KR101035427B1 KR1020100131763A KR20100131763A KR101035427B1 KR 101035427 B1 KR101035427 B1 KR 101035427B1 KR 1020100131763 A KR1020100131763 A KR 1020100131763A KR 20100131763 A KR20100131763 A KR 20100131763A KR 101035427 B1 KR101035427 B1 KR 101035427B1
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liquid chamber
solution
fluid passage
container
container body
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KR1020100131763A
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문상호
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문상호
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/05—Containers specially adapted for medical or pharmaceutical purposes for collecting, storing or administering blood, plasma or medical fluids ; Infusion or perfusion containers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Pharmacology & Pharmacy (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PURPOSE: A solution container is provided to facilitate the collection of separated cells since keeping solution and collected cells are easily separated. CONSTITUTION: A solution container comprises a container body(100), a containing member(200), a filter member(300), and an opening/closing unit. The container main body comprises an upper liquid chamber(110), a lower liquid chamber(120), and a collection liquid unit(130). The upper liquid chamber comprises an inner space for receiving solution. The lower liquid chamber is formed on the lower part of the upper liquid chamber. The liquid collecting unit is divided into the upper liquid chamber and the lower liquid chamber and comprises a fluid pipe(135). The containing member is coupled with the outer surface of the lower liquid chamber of the container body. The containing member contains the solution flowing in the lower liquid chamber. The filter member is formed inside the container body and is positioned on the upper part of the liquid collecting unit.

Description

솔루션 용기{FILTER CONTAINER FOR EXTRACTING CELLS}Solution container {FILTER CONTAINER FOR EXTRACTING CELLS}

본 발명은 솔루션 용기에 관한 것으로, 특히 상,하부 용기가 서로 착탈 가능하도록 결합되고, 서로 다른 메쉬크기를 갖는 막부재와 필터부재를 구비하여 보존 용액내에 함유된 세포의 분리 작업이 용이하게 이루어지도록 그 구조가 개선된 솔루션 용기에 관한 것이다.The present invention relates to a solution container, and in particular, the upper and lower containers are detachably coupled to each other, and provided with a membrane member and a filter member having different mesh sizes to facilitate separation of cells contained in the storage solution. The structure relates to an improved solution container.

일반적으로, 신체 이상 유무를 검사하기 위해 신체 검사 부위에 해당하는 세포를 채취하고, 채취된 세포를 세포 보존액이 담겨진 솔루션통에 보존한 후에, 세포를 슬라이드에 부착시킨 후에 현미경으로 검사하는 방식을 채택하고 있다.In general, to check for abnormalities in the body, cells corresponding to the physical examination site are collected, and the collected cells are preserved in a solution container containing a cell preservation solution, and the cells are attached to a slide and then examined under a microscope. Doing.

종래에는 솔에 묻은 세포를 액에 분산시키기 위한 솔루션통이 별도로 존재하고, 솔루션통을 흔들어서 세포들이 충분히 액중에 퍼진 상태에서 액을 다른 용기에 부어넣고 필터의 아래에 흡입기구를 밀착하여 액을 흡입하고, 필터에 걸러진 세포들을 슬라이드에 문질러 채취하는 방식을 채택하고 있다.Conventionally, there is a separate solution container for dispersing the cells attached to the brush in the liquid, shake the solution container and pour the liquid into another container while the cells are sufficiently spread in the liquid and inhale the liquid by closely attaching the suction device under the filter. In addition, the filter is filtered by rubbing the cells filtered on the slide.

채취막을 슬라이드에 접근시키기 위해 용기와 본체를 분리하면 용기에 남겨진 혼합액들이 중력에 의해 아래로 쏟아져서 소실된다.When the container and the body are separated to bring the sampling membrane to the slide, the mixed liquid left in the container is poured down by gravity and disappeared.

그런데, 솔루션통으로부터 슬라이드로 세포를 옮기는 과정이 일일이 사용자에 의해 수동으로 이루어져야 하기 때문에 시간이 많이 소요되고, 사용자의 손으로부터 슬라이드로 오염물질이나 불순물이 옮겨질 수 있어서 세포검사의 정확성을 떨어뜨릴 수 있었다.However, the process of moving the cells from the solution container to the slides is time-consuming because it must be done manually by the user, and contaminants or impurities can be transferred from the user's hand to the slides, thereby reducing the accuracy of the cell test. there was.

그리고 솔루션통 내에 채취된 세포 외에 이물질이 함유될 수 있으며, 이 경우 이물질과 세포를 분리하기 쉽지 않으므로, 분리를 위한 별도의 도구가 요구된다.In addition, foreign matter may be contained in addition to the cells collected in the solution container, in which case it is not easy to separate the foreign matter and cells, a separate tool for separation is required.

본 발명의 목적은 보존 용액과 채취된 세포를 용이하게 분리시킬 수 있도록 함과 아울러, 분리된 세포를 용이하게 채취할 수 있도록 그 구조가 개선된 솔루션 용기를 제공하는 데 있다.SUMMARY OF THE INVENTION An object of the present invention is to provide a solution container having an improved structure so that the preservation solution and the collected cells can be easily separated, and the separated cells can be easily collected.

상기한 목적을 달성하기 위한 본 발명은 세포가 담겨진 솔루션 용액이 수용되는 내부 공간을 갖는 상부액실과, 상기 상부액실의 하측에 마련되는 하부액실과, 상기 상부액실과 하부액실을 구분하며 상기 솔루션 용액을 하부로 유도하기 위한 유체통로를 갖는 집액부로 구성되는 용기 본체와;The present invention for achieving the above object is divided into the upper liquid chamber having an inner space for accommodating the solution solution containing the cell, the lower liquid chamber provided below the upper liquid chamber, the upper liquid chamber and the lower liquid chamber and the solution solution A container body composed of a collecting part having a fluid passage for guiding the lower part thereof;

상기 용기 본체의 하부액실 외주면에 착탈 가능하게 결합되며, 상기 하부액실로 유입되는 솔루션 용액이 수용되는 수용부재와;A receiving member detachably coupled to an outer circumferential surface of the lower liquid chamber of the container body and accommodating a solution solution introduced into the lower liquid chamber;

상기 용기 본체의 내부에 수용되고, 상기 집액부의 상부에 배치되는 메쉬 형태의 필터부재와;A filter member having a mesh shape accommodated inside the container body and disposed above the liquid collection part;

일단부가 상기 집액부의 유체통로 내에 배치되고 타단부가 상기 수용부재의 내부에 배치되며 상기 유체통로를 개폐시키는 개폐수단을 구비한 것을 특징으로 한다.One end is disposed in the fluid passage of the liquid collection portion, the other end is disposed in the receiving member, characterized in that it has an opening and closing means for opening and closing the fluid passage.

상기 집액부는 유체통로가 하측으로 갈수록 좁아지는 형태로 형성된 것이다.The collection portion is formed in a shape in which the fluid passage is narrowed toward the lower side.

상기 집액부의 하단부는 상기 하부액실의 중간 위치보다 낮은 위치에 배치되도록 된 것이다.The lower end of the liquid collection part is to be disposed at a position lower than the intermediate position of the lower liquid chamber.

상기 개폐수단은 상기 집액부의 유체통로가 하측으로 갈수록 좁아지게 형성되고,The opening and closing means is formed such that the fluid passage of the liquid collection portion toward the lower side,

상부에 상기 유체통로의 하단부 직경보다 큰 직경을 갖는 마개부와, 하부에 상기 마개부로부터 하측으로 연장되도록 형성되어 상기 유체통로를 관통하도록 봉 형태로 형성되며 외주면에 수직으로 솔루션 용액이 흐르는 통로가 되는 유입홈이 형성된 끼움부로 구성된 마개부재를 구비하여서,A plug portion having a diameter larger than a diameter of the lower end portion of the fluid passage in the upper portion, and formed to extend downward from the plug portion in the lower portion is formed in the shape of a rod to penetrate the fluid passage, and a passage through which the solution solution flows perpendicularly to the outer circumferential surface thereof. By having a stopper member consisting of a fitting portion in which the inlet groove is formed,

상기 수용부재와 하부액실의 결합상태가 해제될 경우 상기 마개부가 상기 유체통로를 밀폐시키는 것이다.When the coupling state of the accommodation member and the lower liquid chamber is released, the stopper part seals the fluid passage.

상기 마개부재의 끼움부 길이는 상기 집액부의 하단부와 하부액실의 단부 사이의 간격보다 더 길게 형성된 것이다.The length of the fitting portion of the closure member is longer than the interval between the lower end of the liquid collection portion and the end of the lower liquid chamber.

본 발명은 외부 공기의 유입으로 인한 시료 오염을 예방하면서 검체 분리 시간을 대폭 단축시킬 수 있도록 한 것인 바, 이에 따르면 본 발명은 검체 세포가 담겨진 솔루션 용액을 외부 오염없이 장시간 보관할 수 있음과 아울러, 간편하게 용액에 함유된 이물질과 검체 세포를 분리시켜 검체 추출에 소요되는 시간을 대폭 단축시킬 수 있는 유용한 효과를 갖는다.The present invention is intended to significantly reduce the sample separation time while preventing sample contamination due to the inflow of external air, according to the present invention can be stored for a long time without the external contamination of the solution solution containing the sample cells, By simply separating the foreign matter and sample cells contained in the solution has a useful effect that can significantly reduce the time required for sample extraction.

도 1은 본 발명에 따른 솔루션 용기의 분해 사시도.
도 2는 본 발명의 결합상태도.
도 3은 본 발명 용기 본체를 나타낸 단면도.
도 4는 본 발명의 마개부재를 나타낸 정면도.
도 5는 본 발명의 세포 흡착 과정을 나타낸 사용상태도.1 is an exploded perspective view of a solution container according to the present invention.
2 is a combined state of the present invention.
3 is a cross-sectional view showing the container body of the present invention.
Figure 4 is a front view showing the closure member of the present invention.
5 is a state diagram showing the cell adsorption process of the present invention.

이하, 본 발명의 바람직한 실시 예를 첨부된 도면을 참조하여 상세히 설명하기로 한다.Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

본 발명에 따른 솔루션 용기는, 도 1 내지 도 5를 참조하여 설명하면, 그 구성은 크게 상,하부가 개구되도록 형성된 용기 본체(100)와, 용기 본체(100)의 하부에 착탈 가능하게 결합되는 수용부재(200)와, 용기 본체의 내부에 배치되어 솔루션 용액에 함유된 이물질을 필터링하기 위한 필터부재(300)와, 용기 본체의 내부에 배치되어 솔루션 용액의 유입을 선택적으로 차단하기 위한 개폐수단으로 대별된다.When the solution container according to the present invention is described with reference to FIGS. 1 to 5, the configuration of the container body 100 is formed so that the upper and lower portions are largely opened, and is detachably coupled to the lower portion of the container body 100. Receiving member 200, the filter member 300 is disposed inside the container body for filtering the foreign matter contained in the solution solution, and the opening and closing means for selectively blocking the inflow of the solution solution is disposed inside the container body It is roughly divided into.

더 상세하게 설명하면, 용기 본체(100)는 세포가 담겨진 솔루션 용액이 수용되는 내부 공간을 갖는 상부액실(110)과, 상부액실(110)의 하측에 마련되는 하부액실(120)과, 상기 상부액실(110)과 하부액실(120)을 구분하며 솔루션 용액을 하부로 유도하기 위한 유체통로(135)를 갖는 집액부(130)로 구성된다.In more detail, the container body 100 includes an upper liquid chamber 110 having an inner space in which a solution solution containing cells is accommodated, a lower liquid chamber 120 provided below the upper liquid chamber 110, and the upper portion. The liquid chamber 110 and the lower liquid chamber 120 is divided into a liquid collecting part 130 having a fluid passage 135 for guiding the solution solution downward.

즉, 용기 본체(100)는 원통형 몸체로 구성되고, 집액부(130)의 상부에 안착되도록 배치된 필터부재(300)를 기준으로 상,하부액실(120)로 구분되는 구조를 갖는다.That is, the container body 100 has a cylindrical body and has a structure divided into upper and lower liquid chambers 120 based on the filter member 300 disposed to be seated on the upper portion of the liquid collecting part 130.

또한, 하부액실(120)은 상부액실(110)의 용적보다 작은 크기를 갖도록 형성되어 있다.In addition, the lower liquid chamber 120 is formed to have a size smaller than the volume of the upper liquid chamber 110.

그리고 하부액실(120)은 외주면에 수나사산이 형성되어 수용부재(200)의 내주면에 형성된 암나사산과 체결 방식으로 착탈 가능하게 결합되어 있다.The lower liquid chamber 120 has a male thread formed on an outer circumferential surface thereof and is detachably coupled to the female thread formed on the inner circumferential surface of the receiving member 200 in a fastening manner.

상부액실(110)의 외주면에는 수나사산이 형성된 나사부(105)가 형성되고, 나사부에 용기 본체(100)의 상부를 밀폐시키기 위한 캡부재(150)가 체결되어 있다.On the outer circumferential surface of the upper liquid chamber 110, a threaded portion 105 having a male thread is formed, and a cap member 150 for sealing the upper portion of the container body 100 is fastened to the threaded portion.

상기 집액부(130)는 하측으로 갈수록 유체통로(135)가 좁아지는 형태로 형성되고, 집액부(130)의 하단부는 하부액실(120)의 중간 위치보다 낮은 위치에 배치되는 구조를 갖는다.The liquid collection part 130 has a structure in which the fluid passage 135 is narrowed toward the lower side, and the lower end of the liquid collection part 130 is disposed at a position lower than an intermediate position of the lower liquid chamber 120.

이는, 수용부재(200)에 수용되는 솔루션 용액의 수위를 조절하기 위함이다.This is to adjust the level of the solution solution accommodated in the receiving member 200.

필터부재(300)는 메쉬형태로 형성되고, 집액부(130)의 상부면에 안착되도록 배치된다.The filter member 300 is formed in a mesh shape and is disposed to be seated on the upper surface of the liquid collecting part 130.

또, 필터부재(300)는 검체 세포는 통과시키고, 솔루션 용액 내에 함유된 이물질은 걸러주는 필터 기능을 수행하도록 5~7㎛의 구멍 크기를 갖는 메쉬 구조를 갖는다.In addition, the filter member 300 has a mesh structure having a pore size of 5 ~ 7㎛ to perform the filter function to pass the sample cells, the foreign matter contained in the solution solution to filter.

개폐수단은 상단부에 유체통로(135)의 하단부 직경보다 큰 직경을 갖도록 형성된 마개부(410)와, 하부에 마개부(410)로부터 하측으로 연장되도록 형성되어 상기 유체통로(135)를 관통하여 수용부재(200)의 내부로 배치되도록 봉 형태로 형성되며 외주면에 수직으로 솔루션 용액이 흐르는 통로가 되는 유입홈(425)이 형성된 끼움부(420)로 구성된 마개부재(400)로 구성된다.The opening and closing means is formed in the upper end of the plug portion 410 formed to have a diameter larger than the diameter of the lower end of the fluid passage 135, and formed to extend downward from the stopper portion 410 in the lower portion is received through the fluid passage 135 It is formed in the shape of a rod to be disposed inside the member 200 and consists of a closure member 400 consisting of a fitting portion 420 is formed with an inlet groove 425 is a passage in which the solution solution flows perpendicular to the outer peripheral surface.

이로 인해, 상기 마개부재(400)는 수용부재(200)와 하부액실(120)의 결합상태가 해제될 경우 상기 마개부(410)가 자중에 의해 하강 동작되어 유체통로(135)를 밀폐시키는 구조를 갖는다.For this reason, the closure member 400 has a structure in which the closure portion 410 is moved downward by its own weight when the coupling state of the accommodation member 200 and the lower liquid chamber 120 is released to seal the fluid passage 135. Has

즉, 수용부재(200)가 하부액실(120)에 결합된 상태에서는 마개부재(400)의 하단부가 수용부재(200)의 바닥면에 접촉되면서 상승되어 유체통로(135)를 개방시키게 되고, 이로 인해 상부액실(110)에 담겨진 솔루션 용액이 하측의 하부액실(120)로 유입 가능한 상태가 된다.That is, in the state in which the receiving member 200 is coupled to the lower liquid chamber 120, the lower end of the plug member 400 comes into contact with the bottom surface of the receiving member 200 to open the fluid passage 135. Due to this, the solution solution contained in the upper liquid chamber 110 is allowed to flow into the lower liquid chamber 120 at the lower side.

이를 위해 마개부재(400)의 끼움부(420) 길이는 상기 집액부(130)의 하단부와 하부액실(120)의 단부 사이의 간격보다 더 길게 형성되어 있다.To this end, the length of the fitting portion 420 of the plug member 400 is longer than the interval between the lower end of the liquid collecting part 130 and the end of the lower liquid chamber 120.

이러한 구성을 갖는 본 발명의 작용을 설명하면 다음과 같다.The operation of the present invention having such a configuration will now be described.

본 발명에 의한 솔루션 용기는, 캡부재(150)를 회전시켜 용기 본체(100)의 상부를 개방시킨 후에, 개방된 상부를 통해 검체 세포가 담겨진 솔루션 용액을 용기 본체(100)의 상부액실(110) 내부로 공급한다.In the solution container according to the present invention, after rotating the cap member 150 to open the upper part of the container body 100, the solution solution containing the sample cells through the opened upper part is the upper liquid chamber 110 of the container body 100. Supply inside.

이후에, 상부액실(110)에 공급된 솔루션 용액은 마개부재(400)가 상승되어 개방되어 있으므로, 필터부재(300)를 통과하면서 마개부재(400)의 유입홈(425)을 따라 하측의 하부액실(120)로 유입된다.Subsequently, the solution solution supplied to the upper liquid chamber 110 is opened and closed by the plug member 400, and thus passes through the filter member 300 and is lower along the inlet groove 425 of the plug member 400. It flows into the liquid chamber 120.

이때, 솔루션 용액이 필터부재(300)를 통과하는 도중에 솔루션 용액에 함유된 불순물 등의 이물질은 필터부재(300)를 통과하지 못하고 상부액실(110)에 남아 있게 되고, 검체 세포 및 솔루션 용액만 필터부재(300)를 통과하게 된다.At this time, foreign matters such as impurities contained in the solution solution while the solution solution passes through the filter member 300 do not pass through the filter member 300 and remain in the upper liquid chamber 110, and filter only the sample cells and the solution solution. It passes through the member 300.

하부액실(120)로 유입된 솔루션 용액 및 검체 세포는 하부에 결합된 수용부재(200)의 내부에 담겨지게 된다.The solution solution and the sample cells introduced into the lower liquid chamber 120 are contained in the receiving member 200 coupled to the lower portion.

이후에, 수용부재(200)를 회전 동작시켜 수용부재(200)와 하부액실(120)의 결합상태를 해제하여 분리한다.Thereafter, the receiving member 200 is rotated to release the combined state of the receiving member 200 and the lower liquid chamber 120 to separate them.

이때, 수용부재(200)와 하부액실(120)의 결합상태가 해제됨에 따라, 마개부재(400)가 자중에 의해 하강 동작되고, 이로 인해 마개부재(400)의 마개부(410)가 유체통로(135)의 하부면에 접촉되면서 유체통로(135)를 밀폐시키게 되고, 상부액실(110)의 솔루션 용액이 하부액실(120)로 유입되는 것을 차단하게 된다.At this time, as the coupling state of the receiving member 200 and the lower liquid chamber 120 is released, the plug member 400 is lowered by its own weight, and thus the plug portion 410 of the plug member 400 is fluid passage. The fluid passage 135 is closed while being in contact with the lower surface of the 135, and the solution solution of the upper liquid chamber 110 is blocked from entering the lower liquid chamber 120.

한편, 분리된 수용부재(200)의 상부에서 내부에 담겨진 솔루션 용액측으로 진공 흡착기(500)를 집어 넣은 후에, 솔루션 용액에 함유된 검체 세포를 흡입하여 별도의 장소로 이송시킨다.On the other hand, after the vacuum adsorber 500 is inserted from the upper portion of the separated receiving member 200 into the solution solution contained therein, the sample cells contained in the solution solution are sucked and transferred to a separate place.

이어서, 진공 흡착기(500)에 붙어 있는 검체 세포를 슬라이드의 표면에 묻혀서 확진하는 절차를 갖는다.Subsequently, a procedure is performed in which the sample cells attached to the vacuum adsorber 500 are buried and confirmed on the surface of the slide.

이후에, 수용부재(200)를 다시 하부액실(120)의 외주면과 결합시키면, 마개부재(400)의 하단부가 수용부재(200)의 바닥면에 접촉되면서 상승되어 유체통로(135)를 개방시키게 되므로, 상부액실(110)의 솔루션 용액이 집액부(130)와 마개부재(400)의 유입홈(425)을 통해 하부액실(120)과 수용부재(200)의 내부로 유입된다.Thereafter, when the receiving member 200 is coupled to the outer circumferential surface of the lower liquid chamber 120 again, the lower end of the cap member 400 is raised while contacting the bottom surface of the receiving member 200 to open the fluid passage 135. Therefore, the solution solution of the upper liquid chamber 110 is introduced into the lower liquid chamber 120 and the receiving member 200 through the inflow groove 425 of the collecting part 130 and the closure member 400.

따라서, 본 발명은 세포 보존을 위한 솔루션 용액 내에 함유된 세포와 이물질을 간편하게 분리시킬 수 있으며, 분리된 세포를 용이하게 슬라이드에 채취할 수 있는 이점을 갖는다.Therefore, the present invention can easily separate the cells and foreign matter contained in the solution solution for cell preservation, and has the advantage that the separated cells can be easily collected on the slide.

* 도면의 주요부분에 대한 부호의 설명 *
100 : 용기 본체 110 : 상부액실
120 : 하부액실 130 : 집액부
135 : 유체통로 150 : 캡부재
200 : 수용부재 300 : 필터부재
400 : 마개부재 410 : 마개부
420 : 끼움부 425 : 유입홈Explanation of symbols on the main parts of the drawings
100: container body 110: upper liquid chamber
120: lower liquid chamber 130: liquid collection portion
135: fluid passage 150: cap member
200: receiving member 300: filter member
400: stopper member 410: stopper
420: fitting part 425: inlet groove

Claims (5)

세포가 담겨진 솔루션 용액이 수용되는 내부 공간을 갖는 상부액실과, 상기 상부액실의 하측에 마련되는 하부액실과, 상기 상부액실과 하부액실을 구분하며 상기 솔루션 용액을 하부로 유도하기 위한 유체통로를 갖는 집액부로 구성되는 용기 본체와;
상기 용기 본체의 하부액실 외주면에 착탈 가능하게 결합되며, 상기 하부액실로 유입되는 솔루션 용액이 수용되는 수용부재와;
상기 용기 본체의 내부에 수용되고, 상기 집액부의 상부에 배치되는 메쉬 형태의 필터부재와;
일단부가 상기 집액부의 유체통로 내에 배치되고 타단부가 상기 수용부재의 내부에 배치되며 상기 유체통로를 개폐시키는 개폐수단을 구비한 것을 특징으로 하는 솔루션 용기.An upper liquid chamber having an inner space for accommodating a solution solution containing cells, a lower liquid chamber provided below the upper liquid chamber, and a fluid passage for separating the upper liquid chamber and the lower liquid chamber to guide the solution solution downward. A container body composed of a collecting part;
A receiving member detachably coupled to an outer circumferential surface of the lower liquid chamber of the container body and accommodating a solution solution introduced into the lower liquid chamber;
A filter member having a mesh shape accommodated inside the container body and disposed above the liquid collection part;
And an opening and closing means, one end of which is disposed in the fluid passage of the liquid collecting part and the other end of which is disposed inside the receiving member, and has opening and closing means for opening and closing the fluid passage. 청구항 1에 있어서,
상기 집액부는 유체통로가 하측으로 갈수록 좁아지는 형태로 형성된 것을 특징으로 하는 솔루션 용기.The method according to claim 1,
The liquid container is a solution container, characterized in that the fluid passage is formed in a narrow shape toward the lower side. 청구항 1에 있어서,
상기 집액부의 하단부는 상기 하부액실의 중간 위치보다 낮은 위치에 배치되도록 된 것을 특징으로 하는 솔루션 용기.The method according to claim 1,
And a lower end portion of the liquid collection part is arranged at a position lower than an intermediate position of the lower liquid chamber. 청구항 1 또는 청구항 3에 있어서,
상기 개폐수단은 상기 집액부의 유체통로가 하측으로 갈수록 좁아지게 형성되고,
상부에 상기 유체통로의 하단부 직경보다 큰 직경을 갖는 마개부와, 하부에 상기 마개부로부터 하측으로 연장되도록 형성되어 상기 유체통로를 관통하도록 봉 형태로 형성되며 외주면에 수직으로 솔루션 용액이 흐르는 통로가 되는 유입홈이 형성된 끼움부로 구성된 마개부재를 구비하여서,
상기 수용부재와 하부액실의 결합상태가 해제될 경우 상기 마개부가 상기 유체통로를 밀폐시키는 것을 특징으로 하는 솔루션 용기.The method according to claim 1 or 3,
The opening and closing means is formed such that the fluid passage of the liquid collection portion toward the lower side,
A plug portion having a diameter larger than a diameter of the lower end portion of the fluid passage in the upper portion, and formed to extend downward from the plug portion in the lower portion is formed in the shape of a rod to penetrate the fluid passage, and a passage through which the solution solution flows perpendicularly to the outer circumferential surface thereof. By having a stopper member consisting of a fitting portion in which the inlet groove is formed,
And the plug portion closes the fluid passage when the coupling state between the accommodation member and the lower liquid chamber is released. 청구항 4에 있어서,
상기 마개부재의 끼움부 길이는 상기 집액부의 하단부와 하부액실의 단부 사이의 간격보다 더 길게 형성된 것을 특징으로 하는 솔루션 용기.The method according to claim 4,
The length of the fitting portion of the closure member is formed longer than the interval between the lower end of the liquid collection portion and the end of the lower liquid chamber.
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